##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513894
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.640455035474243	31.0	31.0	34.0	30.0	34.0
2	31.75912542275255	31.0	31.0	34.0	30.0	34.0
3	31.777697346145313	31.0	31.0	34.0	30.0	34.0
4	35.58460110450793	37.0	35.0	37.0	33.0	37.0
5	32.92343557231647	37.0	35.0	37.0	28.0	37.0
6	33.986368784223984	37.0	35.0	37.0	26.0	37.0
7	35.332385667083095	37.0	35.0	37.0	32.0	37.0
8	35.20217204326184	37.0	35.0	37.0	32.0	37.0
9	37.28060261454697	39.0	37.0	39.0	34.0	39.0
10	36.63627323922832	39.0	35.0	39.0	32.0	39.0
11	36.5143298034225	39.0	35.0	39.0	32.0	39.0
12	36.9285008192351	39.0	37.0	39.0	33.0	39.0
13	36.92759790929647	39.0	37.0	39.0	33.0	39.0
14	37.95047422231044	40.0	37.0	41.0	33.0	41.0
15	38.038716155471754	40.0	37.0	41.0	33.0	41.0
16	38.08521407138437	40.0	37.0	41.0	33.0	41.0
17	37.95953834837535	40.0	37.0	41.0	33.0	41.0
18	37.88570210977361	39.0	37.0	40.0	33.0	41.0
19	37.63808295095876	39.0	37.0	40.0	33.0	41.0
20	37.260182060891935	39.0	35.0	40.0	32.0	41.0
21	37.306399763375325	39.0	35.0	40.0	33.0	41.0
22	37.32085605202629	39.0	35.0	40.0	33.0	41.0
23	36.973977123686986	39.0	35.0	40.0	32.0	41.0
24	36.754682872343324	39.0	35.0	40.0	31.0	41.0
25	36.606496670519604	38.0	35.0	40.0	31.0	41.0
26	36.85307865046099	39.0	35.0	40.0	32.0	41.0
27	36.70032925077934	39.0	35.0	40.0	32.0	41.0
28	36.82221625471401	39.0	35.0	40.0	32.0	41.0
29	36.87425422363367	39.0	35.0	40.0	32.0	41.0
30	36.594780246509984	39.0	35.0	40.0	31.0	41.0
31	36.414118475794616	39.0	35.0	40.0	31.0	41.0
32	35.939740491229706	39.0	35.0	40.0	29.0	41.0
33	35.70126134961685	39.0	35.0	40.0	26.0	41.0
34	35.321648044149185	39.0	35.0	40.0	23.0	41.0
35	35.049802099265605	39.0	35.0	40.0	21.0	41.0
36	34.912100549918854	39.0	35.0	40.0	20.0	41.0
37	34.74319995952472	38.0	35.0	40.0	19.0	41.0
38	34.749092225244894	38.0	35.0	40.0	20.0	41.0
39	34.63532362705149	38.0	35.0	40.0	18.0	41.0
40	34.5590121698249	38.0	34.0	40.0	18.0	41.0
41	34.38535962669344	38.0	34.0	40.0	18.0	41.0
42	34.43001864197675	38.0	34.0	40.0	18.0	41.0
43	34.33356100674458	38.0	34.0	40.0	16.0	41.0
44	34.29839032952321	38.0	34.0	40.0	15.0	41.0
45	34.38757603708158	38.0	34.0	40.0	18.0	41.0
46	34.26908662097631	38.0	34.0	40.0	17.0	41.0
47	34.12015318334131	38.0	34.0	40.0	15.0	41.0
48	34.099043771672754	38.0	34.0	40.0	15.0	41.0
49	34.118150824878285	38.0	34.0	40.0	15.0	41.0
50	33.96544423558166	38.0	34.0	40.0	15.0	41.0
51	33.02793766807941	37.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	5.0
10	5.0
11	9.0
12	11.0
13	26.0
14	31.0
15	36.0
16	67.0
17	140.0
18	287.0
19	536.0
20	1139.0
21	2008.0
22	3342.0
23	4684.0
24	6355.0
25	8678.0
26	10764.0
27	11059.0
28	10061.0
29	9478.0
30	9867.0
31	11529.0
32	14837.0
33	19594.0
34	27902.0
35	39961.0
36	51871.0
37	72030.0
38	107968.0
39	89582.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.62237348558263	21.272285724293337	21.429127407597676	17.676213382526356
2	36.838336310601015	28.3589611865482	21.950051956240003	12.85265054661078
3	25.12055015236605	28.228194919574857	34.574639906284176	12.076615021774918
4	21.96347884972387	24.029663704966396	40.35384729146478	13.653010153844955
5	22.582672691255397	31.136382211117468	31.88128291048348	14.399662187143653
6	19.505189786220505	36.50324775148182	33.244988266062656	10.746574196235022
7	62.842531728333086	7.262392633500293	25.380136759720877	4.51493887844575
8	61.024063328235	11.211066873713255	21.307506995606097	6.45736280244564
9	56.69807392185937	8.143119008978505	23.28184411571258	11.876962953449544
10	31.289332041238076	20.86617084457106	33.177659205984114	14.66683790820675
11	22.673352870436318	23.859395128178186	33.970429699510014	19.496822301875483
12	17.765920598411345	20.58790334193433	40.612071750205295	21.03410430944903
13	20.59451949234667	23.0187548404924	42.70394283646044	13.682782830700493
14	17.665316193611915	28.06181819597037	37.07379342821671	17.199072182200997
15	14.979353718860311	24.569463741549814	42.34472478760211	18.106457751987765
16	14.79332313667799	25.37819083312901	36.626035719428515	23.202450310764476
17	15.123936064635899	27.19296197270254	37.19405169159399	20.489050271067573
18	14.84002537488276	25.409909436576417	40.006888580135204	19.743176608405623
19	14.557866019062295	27.7249783029185	37.599971978657074	20.117183699362126
20	16.216768438627422	28.006943066079774	39.65778934955458	16.118499145738227
21	15.067115008153431	30.16964588027881	38.557173269195594	16.206065842372162
22	12.880477296874455	25.78741919539827	37.605031387795925	23.727072119931346
23	14.380981291861747	27.53797475744025	36.76400191479176	21.317042035906237
24	16.138153004316067	25.78352734221454	36.277909452143824	21.800410201325565
25	12.902271674703345	31.144555102803302	34.20802733637676	21.745145886116592
26	13.42436377930079	26.574157316489394	40.056509708227765	19.94496919598205
27	18.280618181959703	26.292970923964866	36.26545552195589	19.160955372119542
28	12.127209113163415	26.539325230495002	38.19250662588004	23.140959030461534
29	15.492105375816804	23.88585972982755	38.564762382903865	22.057272511451778
30	18.346001315446376	28.507240792848332	33.471299528696576	19.675458363008712
31	14.97312675376634	30.053084877426084	30.139678610764086	24.83410975804349
32	16.703055493934546	32.518573869319354	30.074295477277413	20.704075159468687
33	18.577177394559968	26.598286806228522	28.18460616391707	26.63992963529444
34	15.130357622389054	29.004814222388276	31.46329787855083	24.401530276671842
35	18.054890697303335	29.45451785776833	30.022339237274615	22.46825220765372
36	21.095400997092785	27.44943509751038	29.120791447263443	22.334372458133387
37	18.31545026795409	30.823282622486346	30.000934044764094	20.860333064795462
38	16.538624696921932	31.025075210062774	25.55059992916827	26.88570016384702
39	19.11503150455152	30.511545182469536	27.93397081888483	22.439452494094112
40	17.398140472548814	29.26284408846961	30.459783535125922	22.87923190385566
41	19.705036447205064	30.867260563462505	26.827127773431876	22.600575215900555
42	21.32677166886556	27.07834689644168	31.33622887210203	20.25865256259073
43	23.408134751524635	24.32155269374618	30.152716318929585	22.1175962357996
44	19.433774280299048	24.838390796545593	29.335816335664553	26.392018587490806
45	18.966557305592204	24.246634519959372	28.814113416385478	27.97269475806295
46	22.60368869844754	27.66951939505034	29.022522154374247	20.70426975212787
47	16.246735708142147	27.936500523454256	34.673492977150936	21.14327079125267
48	18.67739261404103	27.170583816896094	30.292044662907138	23.859978906155742
49	20.43826158701989	23.193109863123524	34.169108804539455	22.19951974531713
50	20.116016143407006	22.114093567934244	31.433136016376917	26.33675427228183
51	18.33724464578298	22.268794731987533	28.694049745667392	30.699910876562093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3515.0
1	4163.0
2	4811.0
3	10907.5
4	17004.0
5	11690.0
6	6376.0
7	6273.5
8	6171.0
9	6436.5
10	6702.0
11	7106.0
12	7510.0
13	7376.0
14	7242.0
15	6845.0
16	6448.0
17	5889.0
18	5330.0
19	5258.5
20	5187.0
21	4676.5
22	4166.0
23	4177.0
24	4188.0
25	4390.0
26	4586.5
27	4581.0
28	4979.5
29	5378.0
30	5825.5
31	6273.0
32	7033.0
33	7793.0
34	8717.5
35	9642.0
36	10432.5
37	11223.0
38	12314.5
39	13406.0
40	15420.0
41	17434.0
42	21031.5
43	24629.0
44	29589.5
45	34550.0
46	51480.0
47	68410.0
48	67665.5
49	66921.0
50	64250.0
51	61579.0
52	49333.0
53	37087.0
54	28928.0
55	20769.0
56	16624.0
57	12479.0
58	10256.0
59	8033.0
60	6818.5
61	5604.0
62	4621.5
63	3639.0
64	2950.0
65	2261.0
66	1797.0
67	1333.0
68	990.0
69	647.0
70	541.5
71	436.0
72	327.0
73	218.0
74	189.0
75	108.0
76	56.0
77	54.0
78	52.0
79	47.5
80	43.0
81	25.5
82	8.0
83	6.0
84	4.0
85	3.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513894.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.431835022850784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13292472198832	34.58858475776491
2	10.38444205908277	9.435685168652027
3	4.222415755083269	5.754962879484869
4	2.2819426217649106	4.1469136289454
5	1.5088437496249447	3.427477015411003
6	1.0492318635565536	2.860111735549178
7	0.7803854806607109	2.481804108812387
8	0.6386994576798306	2.3213830712395462
9	0.4676929104850437	1.9123332435462086
>10	2.41528786372053	17.32169064389485
>50	0.06210695896717907	1.9252219782254605
>100	0.046905955020777663	3.883987846395846
>500	0.0030402007883837375	1.1083295942494142
>1k	0.005211772780086407	5.833479815629283
>5k	8.686287966810678E-4	2.998034512199679
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9537	1.8558301906618873	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	5657	1.100810673018171	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	4946	0.9624552923365519	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	4869	0.9474716575791895	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	3546	0.690025569475417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	3276	0.6374855514950554	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2785	0.5419405558344718	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1947	0.3788719074361639	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	1860	0.3619423460869362	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT	1841	0.35824508556239226	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	1234	0.24012734143617165	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	1140	0.22183563147263832	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.2161924443562291	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	1009	0.19634399311920359	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	982	0.1910899913211674	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTC	955	0.1858359895231312	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTA	938	0.1825279143169603	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	760	0.1478904209817589	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	710	0.13816078802243265	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.1276527844263603	No Hit
ACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	616	0.1198690780588993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39599606144457805	0.0
2	0.0	0.0	0.0	1.9245213993547308	0.0
3	0.0	0.0	0.0	2.564147470100838	0.0
4	0.0	0.0	0.0	3.6135856810937663	0.0
5	0.0	0.0	0.0	6.800040475273111	0.0
6	0.0	0.0	0.0	8.215507478195892	0.0
7	0.0	0.0	0.0	9.783924311239282	0.0
8	0.0	0.0	0.0	12.086344654734244	0.0
9	0.0	0.0	0.0	12.797580824060994	0.0
10	0.0	0.0	0.0	16.081137355174413	0.0
11	0.0	0.0	0.0	19.819262338147556	0.0
12	0.0	0.0	0.0	23.484609666584937	0.0
13	0.0	0.0	0.0	24.49240504851195	0.0
14	0.0	0.0	0.0	24.91681163819776	0.0
15	0.0	0.0	0.0	25.833732248284665	0.0
16	0.0	0.0	0.0	27.57981217916535	0.0
17	0.0	0.0	0.0	29.748547365799173	0.0
18	0.0	0.0	0.0	31.87252624082009	0.0
19	0.0	0.0	0.0	33.69702701335295	0.0
20	0.0	0.0	0.0	34.98990064098822	0.0
21	0.0	0.0	0.0	36.49001545065714	0.0
22	0.0	0.0	0.0	38.13840986662619	0.0
23	0.0	0.0	0.0	39.60953037007632	0.0
24	0.0	0.0	0.0	40.691076369835024	0.0
25	0.0	0.0	0.0	41.49785753482236	0.0
26	0.0	0.0	0.0	42.20714777755724	0.0
27	0.0	0.0	0.0	42.82030924665398	0.0
28	1.9459265918652486E-4	0.0	0.0	43.423741082791395	0.0
29	1.9459265918652486E-4	0.0	0.0	44.01724869331029	0.0
30	1.9459265918652486E-4	0.0	0.0	44.596356447049395	0.0
31	1.9459265918652486E-4	0.0	0.0	45.111248623256934	0.0
32	1.9459265918652486E-4	0.0	0.0	45.58761145294555	0.0
33	1.9459265918652486E-4	0.0	0.0	46.02719627004791	0.0
34	1.9459265918652486E-4	0.0	0.0	46.42747336999459	0.0
35	1.9459265918652486E-4	0.0	0.0	46.83242069376175	0.0
36	1.9459265918652486E-4	0.0	0.0	47.225108680000154	0.0
37	1.9459265918652486E-4	0.0	0.0	47.6100129598711	0.0
38	1.9459265918652486E-4	0.0	0.0	48.004257687383	0.0
39	1.9459265918652486E-4	0.0	0.0	48.34712995286966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	30	2.1631495E-6	45.000004	6
CGGCGAG	80	0.0	45.000004	31
CGCGGGA	20	7.02964E-4	45.000004	4
ATGGACG	20	7.02964E-4	45.000004	1
TTGTACG	20	7.02964E-4	45.000004	1
CCGTCGA	20	7.02964E-4	45.000004	41
TGACCGG	40	6.8030204E-9	45.000004	2
TACTGGC	20	7.02964E-4	45.000004	24
CGGGCCG	20	7.02964E-4	45.000004	6
GTGTAAT	20	7.02964E-4	45.000004	9
GCGATTC	20	7.02964E-4	45.000004	9
ACGTCAG	20	7.02964E-4	45.000004	1
CGTCGAA	20	7.02964E-4	45.000004	42
CAGTCGA	20	7.02964E-4	45.000004	41
AGCTCGG	20	7.02964E-4	45.000004	2
TCGACTG	20	7.02964E-4	45.000004	1
CGTCAGG	20	7.02964E-4	45.000004	2
CGGCGAT	25	3.887785E-5	45.0	31
TCGCAGG	25	3.887785E-5	45.0	2
ACTGCGG	35	1.2101737E-7	45.0	2
>>END_MODULE