SRR2935225.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935225.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395324
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9452	2.39095020793071	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	8091	2.046675638210683	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	7277	1.840768584755795	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	7197	1.8205320193056835	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4588	1.1605670285639123	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2949	0.7459703939047465	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC	2882	0.7290222703402779	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	2139	0.5410751687223645	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2133	0.539557426313606	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGGGAT	2093	0.5294391435885502	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1701	0.4302799728830023	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTC	1559	0.39436006920905387	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	1319	0.3336503728587184	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	1176	0.2974775121166436	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTA	1014	0.25649846708016716	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	919	0.23246754560815938	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	910	0.23019093199502183	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.21349576549867955	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.2094484524086572	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	708	0.1790936042334895	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	622	0.1573392963746193	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	614	0.1553156398296081	No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	601	0.15202719794396496	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.14089708694640346	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTT	462	0.11686616547439568	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGT	458	0.11585433720189009	No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	451	0.11408363772500531	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATG	427	0.10801266808997177	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	410	0.103712397931823	No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	404	0.10219465552306463	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	400	0.10118282725055903	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAAG	30	2.1618744E-6	45.000004	1
AACGTAG	25	3.8861457E-5	45.000004	1
AGGTACA	30	2.1618744E-6	45.000004	1
CGGCGAT	25	3.8861457E-5	45.000004	31
GGGCTAT	30	2.1618744E-6	45.000004	7
GTAGGTT	25	3.8861457E-5	45.000004	4
CGCGAGG	25	3.8861457E-5	45.000004	2
TAACGGG	30	2.1618744E-6	45.000004	3
GTAATAG	25	3.8861457E-5	45.000004	1
ACGATAG	25	3.8861457E-5	45.000004	1
ACGGCCA	30	2.1618744E-6	45.000004	30
CGAATAT	30	2.1618744E-6	45.000004	14
GCGATGT	25	3.8861457E-5	45.000004	9
GCTACGA	30	2.1618744E-6	45.000004	10
CGATCGA	50	2.1827873E-11	45.000004	41
CTAGACG	20	7.027665E-4	45.0	1
ATGGGCG	35	1.2092278E-7	45.0	5
CCCTCCA	20	7.027665E-4	45.0	14
ATTAGCG	20	7.027665E-4	45.0	1
TTGTGCG	20	7.027665E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content