##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935225.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395324
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.586483492021735	31.0	31.0	34.0	30.0	34.0
2	31.69472129190234	31.0	31.0	34.0	30.0	34.0
3	31.697840252552336	31.0	31.0	34.0	30.0	34.0
4	35.556047697584766	37.0	35.0	37.0	33.0	37.0
5	33.130758567655896	37.0	35.0	37.0	28.0	37.0
6	34.06100059697868	37.0	35.0	37.0	28.0	37.0
7	35.269146320486485	37.0	35.0	37.0	32.0	37.0
8	35.01997096052858	37.0	35.0	37.0	32.0	37.0
9	37.04853993180277	39.0	37.0	39.0	33.0	39.0
10	36.7554815796663	39.0	35.0	39.0	32.0	39.0
11	36.63557487023302	39.0	35.0	39.0	32.0	39.0
12	36.93557942346025	39.0	37.0	39.0	33.0	39.0
13	36.86341330149447	39.0	37.0	39.0	33.0	39.0
14	37.74899829001022	40.0	37.0	41.0	33.0	41.0
15	37.85643168641418	40.0	37.0	41.0	33.0	41.0
16	37.92886594287218	40.0	37.0	41.0	33.0	41.0
17	37.80303750847406	39.0	37.0	41.0	33.0	41.0
18	37.726545820643324	39.0	37.0	40.0	33.0	41.0
19	37.494950976920194	39.0	36.0	40.0	33.0	41.0
20	37.10424360777489	39.0	35.0	40.0	32.0	41.0
21	37.145278303366354	39.0	35.0	40.0	32.0	41.0
22	37.14916878307414	39.0	35.0	40.0	32.0	41.0
23	36.77956562212261	39.0	35.0	40.0	31.0	41.0
24	36.53241897785108	38.0	35.0	40.0	31.0	41.0
25	36.313269621879776	38.0	35.0	40.0	31.0	41.0
26	36.65938824862644	38.0	35.0	40.0	32.0	41.0
27	36.51201293116532	38.0	35.0	40.0	31.0	41.0
28	36.64067448472645	38.0	35.0	40.0	31.0	41.0
29	36.72087199360524	39.0	35.0	40.0	32.0	41.0
30	36.47062409567848	38.0	35.0	40.0	31.0	41.0
31	36.33723730408475	38.0	35.0	40.0	31.0	41.0
32	35.91918780544566	38.0	35.0	40.0	30.0	41.0
33	35.834631846283045	38.0	35.0	40.0	29.0	41.0
34	35.63625532474629	39.0	35.0	40.0	27.0	41.0
35	35.453759447946496	38.0	35.0	40.0	25.0	41.0
36	35.23217411540913	38.0	35.0	40.0	24.0	41.0
37	35.18685179751293	38.0	35.0	40.0	24.0	41.0
38	35.0169430644231	38.0	35.0	40.0	23.0	41.0
39	34.874889963675365	38.0	35.0	40.0	23.0	41.0
40	34.84969796926066	38.0	35.0	40.0	23.0	41.0
41	34.60277392720907	38.0	34.0	40.0	21.0	41.0
42	34.7142900506926	38.0	35.0	40.0	22.0	41.0
43	34.570354949358	38.0	34.0	40.0	21.0	41.0
44	34.64539213404701	38.0	35.0	40.0	21.0	41.0
45	34.67923020105028	38.0	35.0	40.0	21.0	41.0
46	34.503285912314965	38.0	34.0	40.0	20.0	41.0
47	34.307509789438534	38.0	34.0	40.0	20.0	41.0
48	34.32604901296152	38.0	34.0	40.0	20.0	41.0
49	34.38276198763546	38.0	34.0	40.0	20.0	41.0
50	34.27135969483259	38.0	34.0	40.0	20.0	41.0
51	33.29154819843976	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	5.0
11	10.0
12	20.0
13	16.0
14	29.0
15	37.0
16	54.0
17	80.0
18	216.0
19	431.0
20	870.0
21	1553.0
22	2426.0
23	3145.0
24	4172.0
25	5359.0
26	6647.0
27	7120.0
28	7114.0
29	7292.0
30	8133.0
31	9761.0
32	12512.0
33	16651.0
34	24524.0
35	35328.0
36	42907.0
37	54172.0
38	82007.0
39	62719.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73867511205998	20.62485454968583	20.899565925671094	16.736904412583094
2	34.41910938875454	32.73492122916898	19.552316580829903	13.293652801246573
3	22.765377260171405	33.54488976130971	31.32595035970495	12.363782618813934
4	21.83601299187502	22.358116380487903	41.22365452135463	14.582216106282441
5	24.166759417591646	28.851018405156275	29.89927249547207	17.082949681780008
6	19.062085782800946	36.163501330554176	33.26461332982566	11.509799556819218
7	56.41954447491172	9.256710951017395	28.59806133702988	5.725683237041009
8	51.98444819945159	15.263176533678704	24.25276482075462	8.499610446115085
9	46.79908125992856	10.726138559763637	24.90109378636258	17.57368639394522
10	29.058949115156175	23.56598638079145	32.679270674181176	14.695793829871196
11	21.761896570913986	22.02067165160729	35.5571632382552	20.660268539223523
12	17.790976515465793	19.237890945148788	41.8183565885198	21.152775950865617
13	20.01295140188807	21.992846374113387	44.64110451174227	13.353097712256275
14	15.145804454068054	29.293693274377475	37.321538788436825	18.238963483117647
15	10.583470773340348	24.241128795620806	46.316945088079656	18.858455342959193
16	11.431635822768161	27.472149426799287	35.97656605720877	25.119648693223784
17	11.275308354666045	27.75040220173832	37.88411530794993	23.090174135645697
18	11.853062298266739	26.330301221276724	40.447835193410974	21.368801287045564
19	11.685351762098936	27.78859871902541	37.51758051623478	23.008469002640872
20	13.50386012485961	30.640942619218663	40.166293976586296	15.688903279335431
21	12.980744907974218	32.06534386983841	39.08945573757222	15.864455484615151
22	10.839463326284262	26.36495633961004	37.36707106069958	25.42850927340612
23	11.89429430037134	29.86335259179812	37.14750432556587	21.094848782264673
24	15.911758456354788	26.6424502433447	33.38375610891319	24.062035191387317
25	10.862482419483765	34.605285790895564	31.74459430745414	22.787637482166527
26	11.431129908631906	26.92045006121561	38.343485343667474	23.30493468648501
27	16.493306755977375	27.75697908550961	34.3027491374164	21.44696502109662
28	9.413797290323886	26.531149133369087	39.16939017110016	24.885663405206866
29	13.937934453764509	24.497627262700973	38.63261527253595	22.931823010998574
30	16.504436866974935	28.494601896166184	32.90844977790369	22.092511458955187
31	16.410336837631917	28.56720057471846	32.64512146998411	22.37734111766551
32	18.479778611973977	33.16899555807388	28.406066922321944	19.945158907630198
33	21.389037852495672	26.24657243172689	27.15721787698192	25.20717183879552
34	21.884074834819035	24.73793647742105	32.63550910139531	20.742479586364603
35	20.86491080733778	25.573200716374416	29.126235695277796	24.435652781010006
36	18.637117908348593	29.24183707541156	32.427831348463535	19.693213667776305
37	17.781111189808865	27.655796258259045	34.1906891562364	20.37240339569568
38	20.945857069138228	29.431301919438233	28.405813965253817	21.217027046169722
39	26.913873177444327	28.294512855278203	27.58471532211553	17.206898645161946
40	29.19478706074005	21.778085823274075	30.70013457316024	18.326992542825632
41	24.66280822818751	28.283129787212513	25.79428519391689	21.259776790683084
42	21.656413473505275	25.20742479586365	34.282512571966286	18.853649158664794
43	20.963311106838947	26.75906345175097	31.289524541894746	20.988100899515334
44	20.578816363286823	22.47902985905232	31.089941415143024	25.852212362517836
45	22.597666724003602	19.918345458408798	30.2675779866641	27.216409830923492
46	28.082281875120152	24.072153474112373	29.77152917606824	18.074035474699237
47	18.235675041232003	24.40023879147231	38.55495745262114	18.809128714674543
48	20.52847790672967	24.621829183151036	31.811627930507637	23.03806497961166
49	21.855237729052625	20.40579372868837	37.78571500844877	19.95325353381024
50	21.67159089759286	19.425585089698576	33.62102983881576	25.28179417389281
51	20.22012324068359	18.894122289565015	28.913498800983493	31.972255668767897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5372.0
1	5792.5
2	6213.0
3	10907.0
4	15601.0
5	9927.0
6	4253.0
7	4615.0
8	4977.0
9	5013.5
10	5050.0
11	5055.5
12	5061.0
13	4816.0
14	4571.0
15	4464.0
16	4357.0
17	4065.5
18	3774.0
19	3557.0
20	3340.0
21	3168.0
22	2996.0
23	3044.5
24	3093.0
25	3196.0
26	3402.0
27	3505.0
28	4003.5
29	4502.0
30	4739.0
31	4976.0
32	5260.5
33	5545.0
34	5960.0
35	6375.0
36	6496.0
37	6617.0
38	7326.5
39	8036.0
40	9649.5
41	11263.0
42	14220.5
43	17178.0
44	20907.0
45	24636.0
46	43456.5
47	62277.0
48	56608.0
49	50939.0
50	48753.5
51	46568.0
52	36775.5
53	26983.0
54	20747.0
55	14511.0
56	11414.0
57	8317.0
58	7109.5
59	5902.0
60	5292.5
61	4683.0
62	4033.0
63	3383.0
64	2831.0
65	2279.0
66	2068.0
67	1857.0
68	1567.5
69	1278.0
70	976.0
71	674.0
72	546.0
73	418.0
74	344.5
75	245.0
76	219.0
77	156.5
78	94.0
79	66.5
80	39.0
81	30.5
82	22.0
83	17.0
84	12.0
85	9.0
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	395324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.8133393599687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73241779630642	27.502555966027614
2	11.015897390983366	8.330957327996954
3	4.727585631861021	5.362973995526184
4	2.6797113617145962	4.053153404291114
5	1.8130646172864546	3.427901382750224
6	1.3020147637779227	2.954011566865444
7	1.036503059678437	2.743554936028664
8	0.8369415003860042	2.531804238282988
9	0.6844464832055096	2.3293086428868346
>10	2.999004449956654	17.652375122256796
>50	0.08960042802893552	2.2944052236216708
>100	0.0665215299002703	4.8588310642307
>500	0.006109120092881967	1.6948157166991713
>1k	0.007466702335744626	6.045433072151384
>5k	0.0027151644857253184	8.217918340384275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9452	2.39095020793071	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	8091	2.046675638210683	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	7277	1.840768584755795	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	7197	1.8205320193056835	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4588	1.1605670285639123	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2949	0.7459703939047465	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC	2882	0.7290222703402779	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	2139	0.5410751687223645	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2133	0.539557426313606	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGGGAT	2093	0.5294391435885502	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1701	0.4302799728830023	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTC	1559	0.39436006920905387	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	1319	0.3336503728587184	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	1176	0.2974775121166436	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTA	1014	0.25649846708016716	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	919	0.23246754560815938	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	910	0.23019093199502183	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.21349576549867955	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.2094484524086572	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	708	0.1790936042334895	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	622	0.1573392963746193	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	614	0.1553156398296081	No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	601	0.15202719794396496	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.14089708694640346	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTT	462	0.11686616547439568	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGT	458	0.11585433720189009	No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	451	0.11408363772500531	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATG	427	0.10801266808997177	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	410	0.103712397931823	No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	404	0.10219465552306463	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	400	0.10118282725055903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31948477704364014	0.0
2	0.0	0.0	0.0	2.090690168064676	0.0
3	0.0	0.0	0.0	2.8844694478453117	0.0
4	0.0	0.0	0.0	4.309882526737562	0.0
5	0.0	0.0	0.0	9.449970151065962	0.0
6	0.0	0.0	0.0	11.164007244690431	0.0
7	0.0	0.0	0.0	13.409507138448463	0.0
8	0.0	0.0	0.0	16.130313363215993	0.0
9	0.0	0.0	0.0	17.10369216136637	0.0
10	0.0	0.0	0.0	21.549412633687812	0.0
11	0.0	0.0	0.0	26.16081998563204	0.0
12	0.0	0.0	0.0	31.23665651465633	0.0
13	0.0	0.0	0.0	32.53989132964353	0.0
14	0.0	0.0	0.0	33.10322672036102	0.0
15	0.0	0.0	0.0	34.313373334277706	0.0
16	0.0	0.0	0.0	36.3170462709069	0.0
17	0.0	0.0	0.0	38.47224049134381	0.0
18	0.0	0.0	0.0	40.65525998927462	0.0
19	0.0	0.0	0.0	42.69409395837339	0.0
20	0.0	0.0	0.0	44.110400582813085	0.0
21	0.0	0.0	0.0	45.60588276957635	0.0
22	0.0	0.0	0.0	47.10743592597464	0.0
23	0.0	0.0	0.0	48.32820673675264	0.0
24	0.0	0.0	0.0	49.20040270765246	0.0
25	0.0	0.0	0.0	49.94257874553531	0.0
26	0.0	0.0	0.0	50.55321710799243	0.0
27	0.0	0.0	0.0	51.16410842751768	0.0
28	0.0	0.0	0.0	51.64801529884348	0.0
29	0.0	0.0	0.0	52.11016786231041	0.0
30	0.0	0.0	0.0	52.655543301190924	0.0
31	0.0	0.0	0.0	53.0261254059961	0.0
32	0.0	0.0	0.0	53.42276208881829	0.0
33	0.0	0.0	0.0	53.777913812467744	0.0
34	0.0	0.0	0.0	54.09385719055762	0.0
35	0.0	0.0	0.0	54.45356214143336	0.0
36	0.0	0.0	0.0	54.7606520221388	0.0
37	0.0	0.0	0.0	55.047505337394135	0.0
38	0.0	0.0	0.0	55.37255516993656	0.0
39	0.0	0.0	0.0	55.69229290404832	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAAG	30	2.1618744E-6	45.000004	1
AACGTAG	25	3.8861457E-5	45.000004	1
AGGTACA	30	2.1618744E-6	45.000004	1
CGGCGAT	25	3.8861457E-5	45.000004	31
GGGCTAT	30	2.1618744E-6	45.000004	7
GTAGGTT	25	3.8861457E-5	45.000004	4
CGCGAGG	25	3.8861457E-5	45.000004	2
TAACGGG	30	2.1618744E-6	45.000004	3
GTAATAG	25	3.8861457E-5	45.000004	1
ACGATAG	25	3.8861457E-5	45.000004	1
ACGGCCA	30	2.1618744E-6	45.000004	30
CGAATAT	30	2.1618744E-6	45.000004	14
GCGATGT	25	3.8861457E-5	45.000004	9
GCTACGA	30	2.1618744E-6	45.000004	10
CGATCGA	50	2.1827873E-11	45.000004	41
CTAGACG	20	7.027665E-4	45.0	1
ATGGGCG	35	1.2092278E-7	45.0	5
CCCTCCA	20	7.027665E-4	45.0	14
ATTAGCG	20	7.027665E-4	45.0	1
TTGTGCG	20	7.027665E-4	45.0	1
>>END_MODULE