SRR2935224.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426585
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11043	2.58869861809487	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3073	0.7203722587526519	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	2849	0.6678622080007501	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	2812	0.6591886728319093	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	2773	0.6500462979242121	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	2769	0.6491086184464996	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	2382	0.5583881289778121	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	2085	0.48876542775765675	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	1535	0.3598344995721837	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1129	0.2646600325843618	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1033	0.2421557251192611	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1020	0.23910826681669536	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	944	0.22129235674015732	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	886	0.2076960043133256	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	858	0.20113224796933787	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT	845	0.19808478966677215	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	773	0.18120655906794658	No Hit
ACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	707	0.16573484768568986	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	623	0.1460435786537267	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	602	0.1411207613957359	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	591	0.13854214283202645	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	540	0.12658672949119168	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	510	0.11955413340834768	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	500	0.11720993471406636	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	477	0.1118182777172193	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	477	0.1118182777172193	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	431	0.1010349637235252	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA	429	0.10056612398466894	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	25	3.886666E-5	45.000004	4
TCCTAAC	25	3.886666E-5	45.000004	26
ATCCGGC	25	3.886666E-5	45.000004	6
TACGAGG	60	0.0	45.000004	2
TGCGAAG	30	2.16228E-6	45.000004	1
CGTTTGG	25	3.886666E-5	45.000004	2
CTTACGC	25	3.886666E-5	45.000004	25
CGGGTGT	20	7.0282933E-4	45.0	6
TAATACG	40	6.7975634E-9	45.0	4
CCGATAC	20	7.0282933E-4	45.0	11
GTCCTAC	20	7.0282933E-4	45.0	22
GCGCCCA	20	7.0282933E-4	45.0	33
TGCGCGG	20	7.0282933E-4	45.0	2
GTGCTAG	20	7.0282933E-4	45.0	1
TACGAAG	45	3.8380676E-10	45.0	1
TGTTCGC	20	7.0282933E-4	45.0	35
AGCGATC	20	7.0282933E-4	45.0	40
GCAATAG	20	7.0282933E-4	45.0	1
CGCCGAT	20	7.0282933E-4	45.0	38
TGAGCGG	20	7.0282933E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content