FastQCFastQC Report
Sat 14 Jan 2017
SRR2935223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252790
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73832.920606036631196No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG40441.599746825428221No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC37031.4648522489022509No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC35031.38573519522133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33251.3153210174453103No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC26471.0471142054669884Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC14200.5617310811345385No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC14010.5542149610348511Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGAAGGT12860.5087226551683215No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.47588907789073936No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT10140.4011234621622691Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT9580.3789706871316112No Hit
GAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT6950.2749317615412002No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC6380.2523834012421377No Hit
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC6370.25198781597373315Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.23616440523754895No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTA5770.22825269986945684No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.22508801772222004No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.20214407215475294No Hit
ACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC4680.18513390561335497Illumina Single End Adapter 1 (95% over 21bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.1716840064875984No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG4180.1653546421931247No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.16495905692472013No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC3340.13212547964713794No Hit
TTCTCTGGGATATTTTTATTACTTGCCATATTTTTAATTACAATAAGGTAT3270.12935638276830572No Hit
TGATTTTTTTCTTTATATCTTTTTACATTCATGAACTTTTAATATAGAATC3230.12777404169468728No Hit
ACTTTTTTTCTTTTAATTTTTGTTTTATTTTTTGTAACCACTGTCTTAATG3080.12184026266861822No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG2850.11274180149531232No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG2670.10562126666402943No Hit
GCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG2600.1028521697851972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGATC850.045.0000042
TAGGGTA351.2068995E-745.0000045
CAATAAG351.2068995E-745.00000441
AGCACCC207.0228364E-445.038
TGATAAG207.0228364E-445.026
CGGCGAA502.1827873E-1145.031
CGAACAC207.0228364E-445.034
ACGCCGG207.0228364E-445.037
CCGATTA253.882144E-545.018
GCGAAGG253.882144E-545.02
GGGTCAT207.0228364E-445.07
AGCTTAG207.0228364E-445.01
TCTTACG253.882144E-545.024
ACCGATT253.882144E-545.017
GCCGGTC253.882144E-545.039
AGGACGG207.0228364E-445.02
AGGACGA207.0228364E-445.044
CCTACCC207.0228364E-445.014
GGATATC207.0228364E-445.08
AGGCGAC207.0228364E-445.031
Produced by FastQC (version 0.11.5)