##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935223.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252790
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68436647019265	31.0	31.0	34.0	30.0	34.0
2	31.78454052771075	31.0	31.0	34.0	30.0	34.0
3	31.802836346374463	33.0	31.0	34.0	30.0	34.0
4	35.634166699632104	37.0	35.0	37.0	33.0	37.0
5	33.004675817872545	37.0	35.0	37.0	28.0	37.0
6	34.033660350488546	37.0	35.0	37.0	27.0	37.0
7	35.296206337276	37.0	35.0	37.0	32.0	37.0
8	35.090031251236205	37.0	35.0	37.0	32.0	37.0
9	37.17764547648245	39.0	37.0	39.0	34.0	39.0
10	36.899303769927606	39.0	37.0	39.0	33.0	39.0
11	36.7302504054749	39.0	37.0	39.0	32.0	39.0
12	37.047141896435775	39.0	37.0	39.0	33.0	39.0
13	36.9198860714427	39.0	37.0	39.0	33.0	39.0
14	37.94531824834843	40.0	37.0	41.0	33.0	41.0
15	38.03053127101547	40.0	37.0	41.0	33.0	41.0
16	38.0977688990862	40.0	37.0	41.0	33.0	41.0
17	37.90218363068159	40.0	37.0	41.0	33.0	41.0
18	37.8313659559318	39.0	37.0	40.0	33.0	41.0
19	37.57723406780332	39.0	36.0	40.0	33.0	41.0
20	37.15322599786384	39.0	35.0	40.0	32.0	41.0
21	37.217279164523916	39.0	35.0	40.0	33.0	41.0
22	37.20839036354286	39.0	35.0	40.0	33.0	41.0
23	36.820918548993234	39.0	35.0	40.0	32.0	41.0
24	36.59683136200008	38.0	35.0	40.0	31.0	41.0
25	36.37920408243997	38.0	35.0	40.0	31.0	41.0
26	36.67323865659243	38.0	35.0	40.0	32.0	41.0
27	36.45871276553661	38.0	35.0	40.0	31.0	41.0
28	36.59968748763796	38.0	35.0	40.0	31.0	41.0
29	36.66003797618577	39.0	35.0	40.0	32.0	41.0
30	36.241821274575734	38.0	35.0	40.0	31.0	41.0
31	36.14869259068792	38.0	35.0	40.0	30.0	41.0
32	35.777950077139124	38.0	35.0	40.0	29.0	41.0
33	35.502721626646625	38.0	35.0	40.0	27.0	41.0
34	35.20421298310851	38.0	35.0	40.0	25.0	41.0
35	35.02184026266862	38.0	35.0	40.0	24.0	41.0
36	34.78927568337355	38.0	35.0	40.0	22.0	41.0
37	34.575548874559914	38.0	35.0	40.0	21.0	41.0
38	34.5792950670517	38.0	35.0	40.0	21.0	41.0
39	34.383618814035366	38.0	34.0	40.0	20.0	41.0
40	34.253111278136004	38.0	34.0	40.0	20.0	41.0
41	34.14435302029352	38.0	34.0	40.0	18.0	41.0
42	34.21042367182246	38.0	34.0	40.0	18.0	41.0
43	34.059535582894895	38.0	34.0	40.0	18.0	41.0
44	33.97822303097433	38.0	34.0	40.0	18.0	41.0
45	34.09157798963567	38.0	34.0	40.0	18.0	41.0
46	34.019257090865935	38.0	34.0	40.0	18.0	41.0
47	33.901519047430675	38.0	33.0	40.0	18.0	41.0
48	33.897677914474464	38.0	33.0	40.0	18.0	41.0
49	33.88952094623996	37.0	33.0	40.0	18.0	41.0
50	33.760528501918586	37.0	33.0	40.0	17.0	41.0
51	32.85329324735947	36.0	32.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	1.0
10	4.0
11	8.0
12	5.0
13	10.0
14	8.0
15	23.0
16	46.0
17	86.0
18	147.0
19	302.0
20	674.0
21	1162.0
22	1614.0
23	2151.0
24	2923.0
25	3620.0
26	4595.0
27	5097.0
28	5154.0
29	5117.0
30	5335.0
31	6119.0
32	7821.0
33	10574.0
34	15311.0
35	24008.0
36	28519.0
37	34361.0
38	48737.0
39	39240.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.303453459393175	20.96760156651766	19.8366232841489	17.892321689940268
2	34.408402231100915	30.632540844178962	20.859606788243205	14.099450136476918
3	22.772657146247873	31.63297598797421	33.801178844099844	11.793188021678072
4	20.64875984018355	23.749752759207247	41.63376715851101	13.967720242098183
5	22.68562838719886	30.108390363542863	31.674908026425097	15.53107322283318
6	17.79540329918114	35.91399976264884	34.334427785909256	11.95616915226077
7	54.523913129475055	9.700541951817716	30.331896040191463	5.443648878515764
8	51.34182523042842	15.765655286997113	24.71379405830927	8.1787254242652
9	46.479686696467425	11.522212112820919	26.162822896475333	15.835278294236321
10	28.403417856719017	24.22010364334032	33.19435104236718	14.182127457573479
11	20.98302939198544	24.364492266308	35.88947347600776	18.7630048656988
12	17.325448000316467	21.720400332291625	40.97551327188575	19.978638395506152
13	18.38324300803038	24.579690652320107	44.11290003560268	12.924166304046839
14	13.930930812136555	29.822382214486332	37.7922386170339	18.45444835634321
15	10.439495233197515	26.347561216820285	45.257723802365604	17.9552197476166
16	10.954547252660312	28.302939198544248	36.516871711697455	24.225641837097985
17	11.457336128802563	29.843348233711776	38.477392301910676	20.221923335574985
18	11.131769452905575	27.221409074726054	41.44586415601883	20.200957316349538
19	11.950235373234701	27.659321966849955	38.541477115392226	21.84896554452312
20	13.75687329403853	29.42798370188694	41.420942284109344	15.39420071996519
21	13.736698445349896	32.29795482416235	38.47778788717908	15.487558843308674
22	11.225523161517465	27.078207207563594	39.229795482416236	22.46647414850271
23	12.583567387950472	29.259859962814982	38.11780529293089	20.03876735630365
24	15.640254756912853	26.334506902962936	36.127615807587325	21.89762253253689
25	11.028126112583568	34.019146326990786	34.249772538470665	20.702955021954985
26	11.75165156849559	27.80529293089125	40.25515249812097	20.187903002492188
27	15.42268285929032	30.618299774516395	35.22686815143004	18.73214921476324
28	10.110368289884883	28.435855848728192	38.59567229716365	22.858103564223267
29	15.746271608845285	24.808338937457968	39.11942719253135	20.325962261165394
30	13.981565726492345	35.90806598362277	33.181692313778235	16.92867597610665
31	14.748605561928873	31.60132916650184	30.128565212231496	23.52150005933779
32	21.308596067882434	31.595395387475772	29.49641995332094	17.59958859132086
33	17.940978677954035	31.607262945527907	27.808062027770085	22.643696348747973
34	16.637129633292457	29.40978677954033	32.440365520788006	21.512718066379207
35	19.62696309189446	30.2836346374461	30.130147553305115	19.959254717354323
36	18.848451283674194	31.592230705328532	30.353653229953714	19.205664781043556
37	18.982950274931763	31.596582143280983	31.26903754104197	18.151430040745282
38	19.792713319355986	30.27730527315163	26.77954032991811	23.15044107757427
39	21.853316982475572	30.995292535305985	29.76067091261522	17.390719569603228
40	20.411804264409195	31.006764508089717	31.497685826179833	17.083745401321256
41	20.414573361288028	31.358835396969813	27.04576921555441	21.180822026187744
42	22.63855374025871	25.450373828078643	32.5175046481269	19.393567783535744
43	24.319395545709877	25.624431346176667	29.480596542584752	20.5755765655287
44	19.00510304996242	27.075438110684757	30.630167332568536	23.289291506784288
45	18.62731911863602	24.92028956841647	29.984176589263818	26.46821472368369
46	24.261640096522804	28.14945211440326	30.287986075398553	17.300921713675383
47	16.302068910953757	27.72182443925788	37.754658016535465	18.221448633252898
48	19.387238419241267	27.01451797935045	31.21721587088097	22.381027730527315
49	20.42208948138771	23.025831718026822	36.98010206099925	19.571976739586216
50	20.992523438427153	22.887772459353613	32.94354998219866	23.17615412002057
51	18.57193718105938	22.31180030855651	30.145575378772893	28.970687131611218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4051.0
1	4350.5
2	4650.0
3	8294.5
4	11939.0
5	7720.5
6	3502.0
7	3584.0
8	3666.0
9	3911.5
10	4157.0
11	4213.5
12	4270.0
13	4030.0
14	3790.0
15	3790.5
16	3791.0
17	3650.0
18	3509.0
19	3223.5
20	2938.0
21	2938.0
22	2938.0
23	2780.0
24	2622.0
25	2891.0
26	3067.5
27	2975.0
28	3170.0
29	3365.0
30	3654.5
31	3944.0
32	4134.0
33	4324.0
34	4490.0
35	4656.0
36	5042.5
37	5429.0
38	5685.0
39	5941.0
40	6493.5
41	7046.0
42	8860.0
43	10674.0
44	12334.5
45	13995.0
46	23916.0
47	33837.0
48	31407.5
49	28978.0
50	27632.0
51	26286.0
52	20268.0
53	14250.0
54	11087.0
55	7924.0
56	6584.0
57	5244.0
58	4644.0
59	4044.0
60	3612.5
61	3181.0
62	2838.5
63	2496.0
64	2230.5
65	1965.0
66	1511.5
67	1058.0
68	941.0
69	824.0
70	721.5
71	619.0
72	498.0
73	377.0
74	280.5
75	147.5
76	111.0
77	70.0
78	29.0
79	29.5
80	30.0
81	20.5
82	11.0
83	6.0
84	1.0
85	4.0
86	7.0
87	4.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	252790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.59721710758168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.132	29.75018115726827
2	10.208	8.084167844683876
3	4.133	4.909658949169052
4	2.3539999999999996	3.728473962849891
5	1.4829999999999999	2.9361336485271816
6	1.121	2.6633088226559436
7	0.849	2.3532626127035794
8	0.745	2.359994139611868
9	0.601	2.1418134733490928
>10	3.211	19.976004086432805
>50	0.086	2.414638299220331
>100	0.058	4.383411933809292
>500	0.008	2.0519277905148825
>1k	0.01	9.323560740151182
>5k	0.001	2.9234625390527555
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7383	2.920606036631196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	4044	1.599746825428221	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	3703	1.4648522489022509	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	3503	1.38573519522133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3325	1.3153210174453103	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	2647	1.0471142054669884	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC	1420	0.5617310811345385	No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	1401	0.5542149610348511	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGAAGGT	1286	0.5087226551683215	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1203	0.47588907789073936	No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	1014	0.4011234621622691	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT	958	0.3789706871316112	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	695	0.2749317615412002	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC	638	0.2523834012421377	No Hit
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	637	0.25198781597373315	Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.23616440523754895	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTA	577	0.22825269986945684	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	569	0.22508801772222004	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.20214407215475294	No Hit
ACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	468	0.18513390561335497	Illumina Single End Adapter 1 (95% over 21bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	434	0.1716840064875984	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	418	0.1653546421931247	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.16495905692472013	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	334	0.13212547964713794	No Hit
TTCTCTGGGATATTTTTATTACTTGCCATATTTTTAATTACAATAAGGTAT	327	0.12935638276830572	No Hit
TGATTTTTTTCTTTATATCTTTTTACATTCATGAACTTTTAATATAGAATC	323	0.12777404169468728	No Hit
ACTTTTTTTCTTTTAATTTTTGTTTTATTTTTTGTAACCACTGTCTTAATG	308	0.12184026266861822	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	285	0.11274180149531232	No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	267	0.10562126666402943	No Hit
GCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	260	0.1028521697851972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43277028363463743	0.0
2	0.0	0.0	0.0	2.60136872502868	0.0
3	0.0	0.0	0.0	3.320542742988251	0.0
4	0.0	0.0	0.0	4.5144190830333475	0.0
5	0.0	0.0	0.0	8.282764349855611	0.0
6	0.0	0.0	0.0	9.642390917362237	0.0
7	0.0	0.0	0.0	11.348945765259701	0.0
8	0.0	0.0	0.0	13.409153843110882	0.0
9	0.0	0.0	0.0	14.085209066814352	0.0
10	0.0	0.0	0.0	17.77997547371336	0.0
11	0.0	0.0	0.0	21.001226314332055	0.0
12	0.0	0.0	0.0	24.8625341192294	0.0
13	0.0	0.0	0.0	25.816290201352903	0.0
14	0.0	0.0	0.0	26.241148779619447	0.0
15	0.0	0.0	0.0	27.261363186834924	0.0
16	0.0	0.0	0.0	28.805332489418095	0.0
17	0.0	0.0	0.0	30.547094426203568	0.0
18	3.955852684046046E-4	0.0	0.0	32.23466118121761	0.0
19	3.955852684046046E-4	0.0	0.0	34.0373432493374	0.0
20	3.955852684046046E-4	0.0	0.0	35.19324340361565	0.0
21	7.911705368092092E-4	0.0	0.0	36.42549151469599	0.0
22	7.911705368092092E-4	0.0	0.0	37.72222002452629	0.0
23	7.911705368092092E-4	0.0	0.0	38.73966533486293	0.0
24	7.911705368092092E-4	0.0	0.0	39.51975948415681	0.0
25	7.911705368092092E-4	0.0	0.0	40.12619170062107	0.0
26	7.911705368092092E-4	0.0	0.0	40.61750860397959	0.0
27	7.911705368092092E-4	0.0	0.0	41.11436370109577	0.0
28	7.911705368092092E-4	0.0	0.0	41.59539538747577	0.0
29	7.911705368092092E-4	0.0	0.0	42.07998734127141	0.0
30	7.911705368092092E-4	0.0	0.0	42.56695280667748	0.0
31	7.911705368092092E-4	0.0	0.0	42.9463190790775	0.0
32	7.911705368092092E-4	0.0	0.0	43.287313580442266	0.0
33	7.911705368092092E-4	0.0	0.0	43.659559318011	0.0
34	7.911705368092092E-4	0.0	0.0	43.978401044345105	0.0
35	7.911705368092092E-4	0.0	0.0	44.34985561137703	0.0
36	7.911705368092092E-4	0.0	0.0	44.62597412872345	0.0
37	7.911705368092092E-4	0.0	0.0	44.9226630800269	0.0
38	7.911705368092092E-4	0.0	0.0	45.24308714743463	0.0
39	7.911705368092092E-4	0.0	0.0	45.552039242058626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGATC	85	0.0	45.000004	2
TAGGGTA	35	1.2068995E-7	45.000004	5
CAATAAG	35	1.2068995E-7	45.000004	41
AGCACCC	20	7.0228364E-4	45.0	38
TGATAAG	20	7.0228364E-4	45.0	26
CGGCGAA	50	2.1827873E-11	45.0	31
CGAACAC	20	7.0228364E-4	45.0	34
ACGCCGG	20	7.0228364E-4	45.0	37
CCGATTA	25	3.882144E-5	45.0	18
GCGAAGG	25	3.882144E-5	45.0	2
GGGTCAT	20	7.0228364E-4	45.0	7
AGCTTAG	20	7.0228364E-4	45.0	1
TCTTACG	25	3.882144E-5	45.0	24
ACCGATT	25	3.882144E-5	45.0	17
GCCGGTC	25	3.882144E-5	45.0	39
AGGACGG	20	7.0228364E-4	45.0	2
AGGACGA	20	7.0228364E-4	45.0	44
CCTACCC	20	7.0228364E-4	45.0	14
GGATATC	20	7.0228364E-4	45.0	8
AGGCGAC	20	7.0228364E-4	45.0	31
>>END_MODULE