##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935222.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2329697
Sequences flagged as poor quality	0
Sequence length	51
%GC	29
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06983697880025	33.0	31.0	34.0	30.0	34.0
2	32.27005271500972	34.0	31.0	34.0	30.0	34.0
3	32.09330097433271	33.0	31.0	34.0	30.0	34.0
4	35.831762242042636	37.0	35.0	37.0	35.0	37.0
5	33.27381586532498	37.0	35.0	37.0	30.0	37.0
6	34.358619168072075	37.0	35.0	37.0	28.0	37.0
7	35.66813838881193	37.0	35.0	37.0	32.0	37.0
8	35.63594965353864	37.0	35.0	37.0	33.0	37.0
9	37.81483729429192	39.0	38.0	39.0	35.0	39.0
10	37.48222408321769	39.0	37.0	39.0	35.0	39.0
11	37.3058642389976	39.0	37.0	39.0	34.0	39.0
12	37.56383770078255	39.0	37.0	39.0	35.0	39.0
13	37.57582380884725	39.0	37.0	39.0	35.0	39.0
14	38.811840338035374	40.0	38.0	41.0	35.0	41.0
15	38.896474949317444	40.0	38.0	41.0	35.0	41.0
16	38.865374338379624	40.0	38.0	41.0	35.0	41.0
17	38.73627557575084	40.0	38.0	41.0	34.0	41.0
18	38.07858747296322	39.0	38.0	40.0	35.0	41.0
19	37.24505547287909	37.0	37.0	40.0	34.0	41.0
20	36.321559842331425	35.0	35.0	40.0	33.0	41.0
21	36.18916837683184	35.0	35.0	40.0	33.0	41.0
22	36.11744316964824	35.0	35.0	40.0	33.0	41.0
23	35.907051432010256	35.0	35.0	40.0	33.0	41.0
24	35.523572378725646	35.0	35.0	39.0	32.0	41.0
25	35.34484269842817	35.0	35.0	39.0	31.0	41.0
26	35.5417537130365	35.0	35.0	39.0	32.0	41.0
27	35.438056536965966	35.0	35.0	39.0	32.0	41.0
28	35.34987682947611	35.0	35.0	40.0	31.0	41.0
29	35.199932866806286	35.0	35.0	40.0	31.0	41.0
30	34.6109026195252	35.0	35.0	40.0	27.0	41.0
31	33.888406088860485	35.0	33.0	40.0	21.0	41.0
32	32.58469062715022	35.0	33.0	40.0	15.0	41.0
33	31.012914554982903	35.0	26.0	40.0	10.0	41.0
34	29.61089832712151	35.0	21.0	40.0	7.0	41.0
35	28.65312699462634	35.0	17.0	40.0	7.0	41.0
36	28.101952743210813	35.0	15.0	40.0	7.0	41.0
37	27.878659327800996	35.0	15.0	40.0	7.0	41.0
38	27.708488700461906	35.0	12.0	40.0	7.0	41.0
39	27.48920911174286	35.0	12.0	39.0	7.0	41.0
40	27.32995793015143	35.0	11.0	39.0	7.0	41.0
41	27.119858505204753	34.0	10.0	39.0	7.0	41.0
42	27.042625714846178	34.0	10.0	39.0	7.0	41.0
43	26.889476185100467	34.0	10.0	39.0	7.0	41.0
44	26.738769891535252	34.0	10.0	39.0	7.0	41.0
45	26.729795763140014	34.0	10.0	39.0	7.0	41.0
46	26.58799577799173	33.0	10.0	39.0	7.0	41.0
47	26.482782095697424	33.0	10.0	39.0	7.0	41.0
48	26.34999873374091	33.0	10.0	39.0	7.0	41.0
49	26.262651752566963	33.0	10.0	39.0	7.0	41.0
50	26.123500609735945	33.0	10.0	39.0	7.0	41.0
51	25.508429207746758	32.0	10.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	9.0
9	24.0
10	38.0
11	64.0
12	74.0
13	127.0
14	163.0
15	340.0
16	652.0
17	1388.0
18	2861.0
19	5586.0
20	9574.0
21	15830.0
22	24494.0
23	38913.0
24	67975.0
25	121715.0
26	173961.0
27	171096.0
28	137496.0
29	102743.0
30	81013.0
31	71953.0
32	70048.0
33	77278.0
34	101248.0
35	145345.0
36	175710.0
37	181788.0
38	272867.0
39	277240.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.3275387314316	11.456167905096672	13.41852610017526	52.79776726329647
2	63.980165661028025	14.464713651603619	13.499051593404635	8.056069093963721
3	15.843047400584712	14.709595282133256	61.7309890513659	7.716368265916125
4	13.757110903263387	13.997313813770631	63.50396639562999	8.741608887335993
5	13.008859091976339	21.95641750837126	56.90465326606851	8.130070133583896
6	13.022938176080409	19.499660256247918	60.425454468971715	7.0519470986999595
7	40.25866883118277	3.7614762778163855	53.67479118529148	2.305063705709369
8	40.596223457385236	4.9531763143447405	52.018481373328804	2.4321188549412223
9	37.65833067562005	5.164019183610573	52.70432163495939	4.473328505809983
10	19.242201882905803	17.88421412741657	56.2056353251088	6.667948664568827
11	13.33293557059137	14.584171246303704	62.10532957719395	9.977563605910984
12	11.627906976744185	13.679718864727903	63.6351422524045	11.057231906123414
13	12.667956390895469	13.795055751885332	64.57899889985693	8.957988957362266
14	10.753630193110949	16.325384803259823	63.11661988662045	9.804365117008778
15	9.848319330797095	15.856826016430464	63.81117372774228	10.483680925030164
16	11.365941579527295	16.58889546580521	61.607196128938654	10.43796682572884
17	11.61889292899463	16.563398587885032	61.60899893848857	10.20870954463177
18	11.195619001097567	15.983795317588513	62.59590839495437	10.224677286359556
19	10.675723066132635	16.787977148959715	61.844651901084134	10.691647883823519
20	11.234636950642079	17.75883301562392	61.98754601993306	9.018984013800937
21	10.866262865943511	17.608341342243218	62.5754765533887	8.949919238424568
22	10.81398138899608	15.389597874745084	62.7695361242256	11.026884612033239
23	10.366026139879994	16.738314038263344	62.09691646596103	10.798743355895638
24	10.365124735105038	16.93027033129201	61.67218312080928	11.032421812793682
25	10.425561779063973	18.360499240888405	60.54688656936932	10.667052410678298
26	10.3936262956084	17.466219856058533	61.28934363567451	10.850810212658555
27	11.191584141628718	17.1711600263897	61.1452905678292	10.491965264152377
28	9.888109913005854	17.5902703227072	61.29337849514336	11.228241269143584
29	11.195404380913054	16.74616913701653	61.13614774796894	10.922278734101473
30	11.291468375501191	18.93525209501493	59.38557675096805	10.387702778515832
31	11.352978520382694	18.556404545312116	58.3235502299226	11.767066704382586
32	12.26704588622469	19.469055417936325	56.54224562249941	11.72165307333958
33	12.626620543358214	20.74471487064627	53.4193931657207	13.209271420274826
34	13.028260756656337	21.599933381894726	50.43175142518533	14.9400544362636
35	14.183089045485314	23.30603507666448	46.930394810999026	15.580481066851181
36	15.640703490625604	24.50001008714867	45.19561985957831	14.663666562647418
37	14.852661097129799	24.531473406198316	45.73856600235997	14.877299494311922
38	15.27383174721863	24.78326580666928	44.62679052254435	15.316111923567743
39	16.584903530373264	24.00844401653949	43.38092035144484	16.025732101642404
40	16.990106438734305	23.647839182520304	42.75856474039328	16.603489638352112
41	17.04406195312094	23.668056403901453	41.36379108527847	17.924090557699135
42	17.543526046520213	23.41411780158536	42.344605328504095	16.697750823390336
43	17.672856169707906	23.13910349715006	42.74620261776532	16.44183771537672
44	16.7246641945283	23.377417750033587	42.08178145054915	17.816136604888964
45	16.248121536835047	22.841682845451576	42.30490059436914	18.605295023344237
46	16.563741980180254	23.26740344345209	42.12869742288375	18.040157153483907
47	14.816476134020862	23.705357391969855	43.633914624949085	17.8442518490602
48	15.422305990864906	23.191256201986782	42.929531179376546	18.456906627771765
49	16.065222215592843	22.129744769384175	43.48179183816608	18.323241176856904
50	15.311948291988188	22.19876662072364	42.91463653857133	19.57464854871685
51	15.109690230102885	21.92242167114436	41.640865743485094	21.32702235526766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6853.0
1	8452.0
2	10051.0
3	159879.5
4	309708.0
5	203922.0
6	98136.0
7	95022.0
8	91908.0
9	92522.5
10	93137.0
11	92761.0
12	92385.0
13	90278.5
14	88172.0
15	83739.0
16	79306.0
17	74840.0
18	70374.0
19	64922.0
20	59470.0
21	55322.0
22	51174.0
23	47704.0
24	44234.0
25	42306.0
26	38735.0
27	37092.0
28	37035.0
29	36978.0
30	37803.5
31	38629.0
32	39918.0
33	41207.0
34	42889.5
35	44572.0
36	46128.5
37	47685.0
38	51462.5
39	55240.0
40	59561.5
41	63883.0
42	69928.0
43	75973.0
44	82837.0
45	89701.0
46	112436.5
47	135172.0
48	129198.5
49	123225.0
50	116305.0
51	109385.0
52	94134.0
53	78883.0
54	68165.0
55	57447.0
56	51305.5
57	45164.0
58	40256.5
59	35349.0
60	30060.0
61	24771.0
62	21429.5
63	18088.0
64	15457.0
65	12826.0
66	10828.0
67	8830.0
68	7224.0
69	5618.0
70	4475.5
71	3333.0
72	2744.5
73	2156.0
74	1858.5
75	1148.0
76	735.0
77	589.5
78	444.0
79	338.0
80	232.0
81	192.0
82	152.0
83	97.5
84	43.0
85	29.5
86	16.0
87	12.5
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2329697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.62806507977512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.55341286661762	33.1335736542271
2	7.598846455995798	6.174528566908316
3	2.924943679924713	3.565044065479745
4	1.582555651935285	2.5718469607677084
5	1.0777743707329563	2.189394363772611
6	0.7639637707704083	1.8623021878470332
7	0.6010112883863599	1.7092548016768367
8	0.46548727023023423	1.5129477686976658
9	0.38367758800709173	1.402927021368294
>10	2.8333839381611954	21.721501146797543
>50	0.14273663644371895	3.940340783113197
>100	0.06192382727689411	4.735145623973285
>500	0.005578887568157151	1.4835524846021009
>1k	0.0041568181880386615	3.614328684289441
>5k	4.375598092672275E-4	1.197383872267152
>10k+	1.0938995231680688E-4	9.185928014211923
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206690	8.87196918740935	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCC	7749	0.3326183619586582	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC	6572	0.28209677052423554	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	6364	0.2731685708484837	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	6257	0.26857569889989985	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4936	0.21187304615149521	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	4177	0.1792937021423816	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGT	3968	0.17032257842972712	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3528	0.15143600219255982	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	3517	0.1509638377866306	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3252	0.13958896800742757	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	3125	0.13413761532079066	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTC	3025	0.12984521163052534	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTT	2928	0.125681580050968	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	2756	0.1182986457037117	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	2741	0.1176547851501719	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	2657	0.11404916605034904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1462018451326502E-4	0.0	0.0	0.09889698102371253	0.0
2	2.1462018451326502E-4	0.0	0.0	0.4144315762951148	0.0
3	2.1462018451326502E-4	0.0	0.0	0.645963831348025	0.0
4	2.1462018451326502E-4	0.0	0.0	0.9654903620513741	0.0
5	2.1462018451326502E-4	0.0	0.0	1.852558508681601	0.0
6	2.1462018451326502E-4	0.0	0.0	2.454138885872283	0.0
7	2.1462018451326502E-4	0.0	0.0	3.048722645047832	0.0
8	2.1462018451326502E-4	0.0	0.0	3.7550376722809875	0.0
9	2.1462018451326502E-4	0.0	0.0	4.159510872014687	0.0
10	2.5754422141591806E-4	0.0	0.0	5.156936717521635	0.0
11	2.5754422141591806E-4	0.0	0.0	6.299660427944063	0.0
12	2.5754422141591806E-4	0.0	0.0	7.2981593743735775	0.0
13	2.5754422141591806E-4	0.0	0.0	7.620948131881528	0.0
14	2.5754422141591806E-4	0.0	0.0	7.785433041292494	0.0
15	2.5754422141591806E-4	0.0	0.0	8.04001550416213	0.0
16	2.5754422141591806E-4	0.0	0.0	8.455691877527421	0.0
17	2.5754422141591806E-4	0.0	0.0	8.994989477172354	0.0
18	2.5754422141591806E-4	0.0	0.0	9.523942383923746	0.0
19	2.5754422141591806E-4	0.0	0.0	10.011301898916468	0.0
20	3.0046825831857104E-4	0.0	0.0	10.374052934780789	0.0
21	3.43392295221224E-4	0.0	0.0	10.776852097075285	0.0
22	3.43392295221224E-4	0.0	0.0	11.21751025991792	0.0
23	3.43392295221224E-4	0.0	0.0	11.617948600182771	0.0
24	3.43392295221224E-4	0.0	0.0	11.920219668051253	0.0
25	3.43392295221224E-4	0.0	0.0	12.165101298580888	0.0
26	3.43392295221224E-4	0.0	0.0	12.397320338224242	0.0
27	3.43392295221224E-4	0.0	0.0	12.627779492354586	0.0
28	3.8631633212387706E-4	0.0	0.0	12.85180004094953	0.0
29	4.2924036902653004E-4	0.0	0.0	13.082817207559609	0.0
30	4.721644059291831E-4	0.0	0.0	13.333064342702077	0.0
31	5.150884428318361E-4	0.0	0.0	13.54944441272835	0.0
32	5.150884428318361E-4	0.0	0.0	13.759471725293032	0.0
33	5.150884428318361E-4	0.0	0.0	13.9563642825655	0.0
34	5.150884428318361E-4	0.0	0.0	14.153771928280802	0.0
35	5.580124797344891E-4	0.0	0.0	14.362253975516987	0.0
36	5.580124797344891E-4	0.0	0.0	14.553265939733794	0.0
37	5.580124797344891E-4	0.0	0.0	14.743676967433963	0.0
38	6.009365166371421E-4	0.0	0.0	14.951429306042803	0.0
39	6.009365166371421E-4	0.0	0.0	15.20897352745872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	20	7.034779E-4	45.000004	2
TACTACG	25	3.892045E-5	44.999996	1
CGTTATT	7605	0.0	44.64497	1
CGTTTTT	101975	0.0	44.582985	1
GTTATTT	8000	0.0	42.946877	2
CCGATGA	1165	0.0	40.55794	18
CGTAAGG	140	0.0	40.17857	2
TACGGCT	690	0.0	39.782608	7
ACGGCTG	690	0.0	39.782608	8
CACGCGA	40	3.4602635E-7	39.375004	41
TACGCTT	40	3.4602635E-7	39.375004	15
GTTTTTT	116565	0.0	39.16677	2
CCGTCGA	100	0.0	38.249996	41
CGTCGAA	100	0.0	38.249996	42
CGATGAA	1270	0.0	38.09055	19
CGACCAC	2095	0.0	37.696896	12
TGCGTAG	90	0.0	37.5	1
CGCGACC	30	1.14028204E-4	37.499996	10
CGCTATC	30	1.14028204E-4	37.499996	1
TCGCTTC	30	1.14028204E-4	37.499996	14
>>END_MODULE