FastQCFastQC Report
Sat 14 Jan 2017
SRR2935220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3416412
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2507087.33834209691337No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC268340.7854439101607184No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG180000.5268685392745371No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC174270.5100965574409644No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167140.4892267091908119No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC98070.2870555424814103No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT94640.27701576976079No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC82540.24159849573177944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73570.21534288019126496No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT62950.1842576363740673No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT57130.16722222027085726No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC51660.15121127077179214No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT49660.14535717589096397No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT47300.1384493439315867No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGT46090.13490761652868566No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG43670.12782416172288355No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGTTCTT43660.1277948912484794No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC41780.12229204206050089No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT40630.11892593750402469No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG34290.10036845673179932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT57850.044.455491
CGTTTTT837050.044.268861
GTTAGCG800.042.1875041
CGATGAA26050.040.5950119
CCGATGA25400.040.48228518
AATAGCG451.9301297E-840.01
GTTTTTT955500.039.1883852
TACGGCT22400.038.772327
GTTATTT68600.038.3746342
ACCACCG34700.037.86743514
GATGAAT29500.037.83050520
CGACCAC47050.037.1094612
TCACGAC2500.036.89999825
GCGTAAG1350.036.6666641
AACGGGA11450.036.550224
CCACCGA35200.036.43465815
TAGCGTA250.002107689436.01
CGCGATC250.002107689436.041
TACGTAG751.8189894E-1236.01
GCGACCA49000.035.67857411
Produced by FastQC (version 0.11.5)