##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3416412
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95573806672029	33.0	31.0	34.0	30.0	34.0
2	32.100195175523325	33.0	31.0	34.0	30.0	34.0
3	32.01541910050661	33.0	31.0	34.0	30.0	34.0
4	35.761441243035094	37.0	35.0	37.0	35.0	37.0
5	33.401596762919695	37.0	35.0	37.0	30.0	37.0
6	34.34330139339166	37.0	35.0	37.0	29.0	37.0
7	35.55764790663421	37.0	35.0	37.0	32.0	37.0
8	35.46289147795992	37.0	35.0	37.0	33.0	37.0
9	37.541106576138944	39.0	37.0	39.0	35.0	39.0
10	37.16311791434991	39.0	37.0	39.0	34.0	39.0
11	37.02941389972872	39.0	37.0	39.0	33.0	39.0
12	37.243181442987556	39.0	37.0	39.0	34.0	39.0
13	37.22064054335367	39.0	37.0	39.0	34.0	39.0
14	38.31406545814732	40.0	38.0	41.0	33.0	41.0
15	38.39442198423375	40.0	38.0	41.0	33.0	41.0
16	38.39238036864406	40.0	38.0	41.0	33.0	41.0
17	38.28880533144129	40.0	38.0	41.0	33.0	41.0
18	37.92751810964251	39.0	37.0	40.0	33.0	41.0
19	37.46872098564225	38.0	37.0	40.0	33.0	41.0
20	36.91179108374517	38.0	35.0	40.0	33.0	41.0
21	36.8250281289259	38.0	35.0	40.0	33.0	41.0
22	36.81282204839463	38.0	35.0	40.0	33.0	41.0
23	36.58072269972123	38.0	35.0	40.0	32.0	41.0
24	36.28519716006149	37.0	35.0	40.0	32.0	41.0
25	36.116283691779564	37.0	35.0	40.0	31.0	41.0
26	36.33327245074657	37.0	35.0	40.0	32.0	41.0
27	36.24417049231767	37.0	35.0	40.0	32.0	41.0
28	36.24366323499625	37.0	35.0	40.0	32.0	41.0
29	36.22478202277711	37.0	35.0	40.0	32.0	41.0
30	35.8203785140668	37.0	35.0	40.0	31.0	41.0
31	35.53699670882786	37.0	35.0	40.0	30.0	41.0
32	34.94411534674389	37.0	35.0	40.0	24.0	41.0
33	34.26224793730967	37.0	34.0	40.0	20.0	41.0
34	33.680362907049854	37.0	33.0	40.0	15.0	41.0
35	33.17558655103659	37.0	33.0	40.0	12.0	41.0
36	32.873688536394326	37.0	33.0	40.0	10.0	41.0
37	32.78787833551691	37.0	33.0	40.0	10.0	41.0
38	32.655045410213994	37.0	33.0	40.0	10.0	41.0
39	32.49628791843607	37.0	32.0	40.0	10.0	41.0
40	32.343400620299896	37.0	31.0	40.0	10.0	41.0
41	32.19004382375428	36.0	31.0	40.0	9.0	41.0
42	32.21573364102456	37.0	31.0	40.0	8.0	41.0
43	32.10994224349991	36.0	31.0	40.0	8.0	41.0
44	32.01824487210559	36.0	31.0	40.0	8.0	41.0
45	31.990295959620795	36.0	31.0	40.0	8.0	41.0
46	31.89434441747658	36.0	31.0	40.0	8.0	41.0
47	31.786825769257337	35.0	30.0	40.0	8.0	41.0
48	31.748216257289812	35.0	30.0	40.0	8.0	41.0
49	31.72949749620362	35.0	30.0	40.0	8.0	41.0
50	31.63422298013237	35.0	30.0	40.0	8.0	41.0
51	30.853746854887525	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	25.0
9	60.0
10	81.0
11	86.0
12	113.0
13	158.0
14	213.0
15	381.0
16	700.0
17	1272.0
18	2451.0
19	4623.0
20	8321.0
21	13769.0
22	21058.0
23	31152.0
24	48727.0
25	80309.0
26	115786.0
27	128006.0
28	118979.0
29	103774.0
30	94039.0
31	95925.0
32	108103.0
33	135605.0
34	197250.0
35	267780.0
36	334238.0
37	368906.0
38	564502.0
39	569857.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3537606120105	14.06700948246289	16.61544333645942	34.963786569067196
2	50.01677198183357	20.37271851287257	18.034563746995385	11.575945758298472
3	22.18722449165967	20.881410087542136	45.5093530873911	11.422012333407094
4	19.570502620878276	18.581716725031995	48.550028509442065	13.29775214464766
5	19.046941645211408	25.774116236566314	41.8824778744484	13.296464243773878
6	18.426641751638854	26.74955479608431	44.18743992235129	10.636363529925548
7	54.79444516644948	5.266841352857911	36.02018726078705	3.9185262199055617
8	54.51049229425491	7.1289996639749535	33.90501496892061	4.455493072849528
9	49.572680344173946	6.608746251915752	35.26029647478114	8.558276929129157
10	27.165195532623116	20.261783414880874	40.537967903168585	12.035053149327423
11	20.766728368826712	17.717652320621752	45.9695142154986	15.546105095052939
12	17.883996426660485	16.098760922277524	49.089541893659195	16.927700757402793
13	18.400122701828703	16.153496709413268	51.35305694980582	14.09332363895221
14	14.728961261112536	19.773347008498977	49.82104031949308	15.676651410895406
15	13.079189512271938	18.85963987949931	51.96396102109464	16.097209587134103
16	15.550202961469516	19.73400749089981	47.813027234420204	16.902762313210467
17	15.320107762178568	20.375791912685003	48.07417840705395	16.22992191808248
18	15.242365382161168	19.390811178511257	48.98498775908761	16.38183568023997
19	15.10376968585756	20.970245977358704	47.17212092686714	16.753863409916605
20	16.07209552009535	22.29174935575686	47.73314810977131	13.903007014376486
21	15.301169765239086	22.766311557271194	47.999831402067436	13.932687275422284
22	14.52316055557702	19.477276159901088	48.59612950662859	17.403433777893298
23	14.807933001054908	21.01956672672968	47.7140637604598	16.458436511755608
24	15.040838165888657	20.892562138290113	46.51060820533355	17.55599149048768
25	14.964383686745041	23.286682051228013	44.860836456492954	16.888097805533995
26	14.865303130887025	20.95587417442627	46.88070408369951	17.2981186109872
27	16.319021242168684	20.34587748784397	46.5901068138152	16.74499445617215
28	14.581730774859706	20.726744900790653	46.64674518178721	18.04477914256243
29	17.4600136049165	19.539710081805122	46.08677173596159	16.913504577316786
30	17.55669398187338	20.75903023405842	46.54854274016132	15.135733043906882
31	18.756900514340778	20.541638420658867	43.87269451108356	16.8287665539168
32	20.482892578529754	20.656144516527867	42.72810187998403	16.13286102495835
33	19.066991920178246	20.042459750170647	43.46138580475657	17.42916252489454
34	19.239073039200193	20.584548936135338	42.73056059983398	17.445817424830494
35	19.01491974621328	21.59839035807157	41.09864384037991	18.288046055335247
36	20.349302133349255	22.49447666147994	41.15756530535544	15.998655899815361
37	19.875471693695022	22.676012143734418	40.80713918578907	16.641376976781487
38	20.289824529360043	22.72542070452861	39.01929860918414	17.96545615692721
39	20.35498060538366	21.782238207803978	40.20287951218998	17.65990167462238
40	19.141280384215957	21.652365112872804	40.94225169563858	18.264102807272657
41	17.82311383989987	22.852893620558646	39.88696328194609	19.437029257595395
42	18.742265277138706	22.39803044831829	40.373321484645295	18.486382789897707
43	19.90190293208196	21.69378283415466	39.90569638556474	18.498617848198638
44	19.858670441387048	21.86431261803319	38.68072703175144	19.596289908828325
45	19.5169376527187	21.201980323216286	38.155409827620325	21.125672196444693
46	20.285843744841078	22.26206909471106	38.637377459158905	18.814709701288955
47	16.918978156030363	22.905053605946822	41.936950227314505	18.23901801070831
48	17.932614684645763	22.420305279339843	40.09329085602088	19.553789179993515
49	18.72435174680337	20.763918403283913	41.19781220766114	19.313917642251578
50	17.85788716349199	20.668174681507967	40.57637076558682	20.897567389413222
51	17.371148444625533	20.458773707620743	39.10997853888817	23.06009930886556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10008.0
1	13209.5
2	16411.0
3	191861.5
4	367312.0
5	225225.5
6	83139.0
7	77795.0
8	72451.0
9	71769.0
10	71087.0
11	68824.5
12	66562.0
13	63739.5
14	60917.0
15	56724.0
16	52531.0
17	49052.5
18	45574.0
19	42185.5
20	38797.0
21	38286.5
22	37776.0
23	36426.5
24	35077.0
25	34716.0
26	37863.0
27	41371.0
28	44448.5
29	47526.0
30	50288.0
31	53050.0
32	56499.5
33	59949.0
34	66501.5
35	73054.0
36	76877.0
37	80700.0
38	83574.5
39	86449.0
40	94675.0
41	102901.0
42	112285.0
43	121669.0
44	134702.0
45	147735.0
46	216174.5
47	284614.0
48	267478.0
49	250342.0
50	255136.0
51	259930.0
52	223435.0
53	186940.0
54	162668.0
55	138396.0
56	124377.0
57	110358.0
58	102963.0
59	95568.0
60	87090.0
61	78612.0
62	72175.0
63	65738.0
64	56677.0
65	47616.0
66	39576.5
67	31537.0
68	27092.0
69	22647.0
70	19015.0
71	15383.0
72	12448.5
73	9514.0
74	7549.0
75	4705.0
76	3826.0
77	2904.5
78	1983.0
79	1368.0
80	753.0
81	549.0
82	345.0
83	265.5
84	186.0
85	121.5
86	57.0
87	51.0
88	45.0
89	30.5
90	16.0
91	10.0
92	4.0
93	7.0
94	10.0
95	6.0
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3416412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.644067259789296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41722121683247	21.88900024341147
2	8.762881233462824	5.020091188817091
3	3.608764353460478	3.101090665957559
4	2.0121195263541574	2.305411481904955
5	1.2630332871492238	1.8089205214227566
6	0.9497795139902282	1.6323328968423652
7	0.7027924320321152	1.409158358495916
8	0.5563495574318799	1.2748891314426218
9	0.4579100490323547	1.1804765619074566
>10	4.586103128957637	29.038269580958843
>50	0.5593434215651067	10.64708509626456
>100	0.11389042831849959	5.573649458388417
>500	0.004958677595884676	0.9928667156604115
>1k	0.0035871284736179	2.5903866621882807
>5k	7.385264504507442E-4	1.573162164436898
>10k+	5.27518893179103E-4	9.96320927190045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	250708	7.33834209691337	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC	26834	0.7854439101607184	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	18000	0.5268685392745371	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC	17427	0.5100965574409644	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16714	0.4892267091908119	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	9807	0.2870555424814103	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	9464	0.27701576976079	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC	8254	0.24159849573177944	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7357	0.21534288019126496	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT	6295	0.1842576363740673	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	5713	0.16722222027085726	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC	5166	0.15121127077179214	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	4966	0.14535717589096397	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	4730	0.1384493439315867	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGT	4609	0.13490761652868566	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	4367	0.12782416172288355	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGTTCTT	4366	0.1277948912484794	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	4178	0.12229204206050089	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT	4063	0.11892593750402469	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	3429	0.10036845673179932	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0489332082898666E-4	0.0	0.0	0.14904525566588572	0.0
2	2.0489332082898666E-4	0.0	0.0	0.6540780210349337	0.0
3	2.0489332082898666E-4	0.0	0.0	0.9055113961665046	0.0
4	2.0489332082898666E-4	0.0	0.0	1.4907452614028986	0.0
5	2.0489332082898666E-4	0.0	0.0	3.202570415980274	0.0
6	2.0489332082898666E-4	0.0	0.0	4.354041608564775	0.0
7	2.0489332082898666E-4	0.0	0.0	5.38919193586722	0.0
8	2.3416379523312763E-4	0.0	0.0	6.615946788619171	0.0
9	2.3416379523312763E-4	0.0	0.0	7.37156408536207	0.0
10	2.3416379523312763E-4	0.0	0.0	9.289980248283872	0.0
11	2.6343426963726857E-4	0.0	0.0	11.153221566953869	0.0
12	2.927047440414095E-4	0.0	0.0	13.008911103227597	0.0
13	2.927047440414095E-4	0.0	0.0	13.613053694929066	0.0
14	2.927047440414095E-4	0.0	0.0	13.965177502010881	0.0
15	2.927047440414095E-4	0.0	0.0	14.43684192655921	0.0
16	3.219752184455505E-4	0.0	0.0	15.118873250650097	0.0
17	3.219752184455505E-4	0.0	0.0	15.841210017995488	0.0
18	3.219752184455505E-4	0.0	0.0	16.53811074308368	0.0
19	3.219752184455505E-4	0.0	0.0	17.169416335032192	0.0
20	3.219752184455505E-4	0.0	0.0	17.6628579925372	0.0
21	3.5124569284969144E-4	0.0	0.0	18.180740496169665	0.0
22	3.5124569284969144E-4	0.0	0.0	18.74744615110824	0.0
23	3.5124569284969144E-4	0.0	0.0	19.210153810488897	0.0
24	3.5124569284969144E-4	0.0	0.0	19.561077528120144	0.0
25	3.5124569284969144E-4	0.0	0.0	19.852026043697308	0.0
26	3.5124569284969144E-4	0.0	0.0	20.11827027887737	0.0
27	3.5124569284969144E-4	0.0	0.0	20.405120928037952	0.0
28	3.805161672538324E-4	0.0	0.0	20.649412307414913	0.0
29	3.805161672538324E-4	0.0	0.0	20.90160671488099	0.0
30	3.805161672538324E-4	0.0	0.0	21.196096957861055	0.0
31	4.097866416579733E-4	0.0	0.0	21.429558261708483	0.0
32	4.097866416579733E-4	0.0	0.0	21.672503199262852	0.0
33	4.097866416579733E-4	0.0	0.0	21.88808024324935	0.0
34	4.390571160621143E-4	0.0	0.0	22.101725435925175	0.0
35	4.390571160621143E-4	0.0	0.0	22.334484248386904	0.0
36	4.6832759046625525E-4	0.0	0.0	22.528752387007188	0.0
37	4.6832759046625525E-4	0.0	0.0	22.74684669179244	0.0
38	4.6832759046625525E-4	0.0	0.0	22.99743122316629	0.0
39	4.975980648703962E-4	0.0	0.0	23.37472763823567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	5785	0.0	44.45549	1
CGTTTTT	83705	0.0	44.26886	1
GTTAGCG	80	0.0	42.187504	1
CGATGAA	2605	0.0	40.59501	19
CCGATGA	2540	0.0	40.482285	18
AATAGCG	45	1.9301297E-8	40.0	1
GTTTTTT	95550	0.0	39.188385	2
TACGGCT	2240	0.0	38.77232	7
GTTATTT	6860	0.0	38.374634	2
ACCACCG	3470	0.0	37.867435	14
GATGAAT	2950	0.0	37.830505	20
CGACCAC	4705	0.0	37.10946	12
TCACGAC	250	0.0	36.899998	25
GCGTAAG	135	0.0	36.666664	1
AACGGGA	1145	0.0	36.55022	4
CCACCGA	3520	0.0	36.434658	15
TAGCGTA	25	0.0021076894	36.0	1
CGCGATC	25	0.0021076894	36.0	41
TACGTAG	75	1.8189894E-12	36.0	1
GCGACCA	4900	0.0	35.678574	11
>>END_MODULE