FastQCFastQC Report
Sat 14 Jan 2017
SRR2935219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402297
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103812.580431869986602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65051.616964580894215No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG62661.557555736184958No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC56771.4111464912738598No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC49171.2222313365498625No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC41171.0233732789456547No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC31500.7830036018165683No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC30560.7596377800480739No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29890.7429834177237216No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT23520.584642689356371No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAACAAT16390.4074104455166208No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT16150.4014447037884946No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC15720.39075608319226846No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT11490.2856098852340435No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC10650.2647297891856017No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG10350.2572726120254439No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC10270.2552840314494018No Hit
AGGAGAAGGTTGTACCTATTTGTTATTTTTTATTATTTTGCATATATATAT10040.24956686229328082No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.221975306800697No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTA8490.21103811363246558No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.19388660616410264No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.1841922758558975No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.17275793754365557No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC6940.17250936497165031No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG5790.14392351919104543No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG5220.1297548825867456No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG5180.12876059229872458No Hit
AGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG4890.12155198771057205No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT4800.1193148345625247No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.11136051225835639No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC4390.10912335911030906No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.10663763339025646No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG4170.10365476252619335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG351.2093005E-745.0000042
TAGAAGG453.8380676E-1045.0000042
TTGTCGG351.2093005E-745.0000042
TTCGTAG700.045.0000041
AGTATAG700.045.0000041
CAAGGCA650.045.00000436
GGTTGTA1550.045.0000048
CATAAAG351.2093005E-745.0000044
GGATTAC650.045.0000048
AGGTCAG207.0278137E-445.01
CGGGTTA207.0278137E-445.06
CGGCGAT207.0278137E-445.031
GGTACAC207.0278137E-445.08
ACTGCAG502.1827873E-1145.01
GTACACC207.0278137E-445.09
CCTAGGT207.0278137E-445.03
CGGCCGC207.0278137E-445.015
TTGTCAA253.8862694E-545.021
GGATCGA207.0278137E-445.010
GTTAGAC950.045.019
Produced by FastQC (version 0.11.5)