FastQCFastQC Report
Sat 14 Jan 2017
SRR2935218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562099
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156422.7827838156623654No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC45880.816226323121016No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC31000.551504272379065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30980.5511484631710784No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC30940.5504368447551055No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG29180.519125634452294No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC24790.44102551329925865No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC21800.3878320367052779No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT17820.3170260043159657No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15090.26845804742580937No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG14830.2638325277219849No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC12530.22291446880353818No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.19178116310472No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC9910.17630346255730753No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTATAGT9300.16545128171371945No Hit
ACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC8260.1469492028984218No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.1416120647786244No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.1373423542827865No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT7610.13538540363886076No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT7230.1286250286871174No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGT7140.12702388725117816No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC6710.11937398927946857No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTG5680.10104981506816416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT302.163515E-645.0000044
CGATGTG302.163515E-645.00000410
GACGAAG302.163515E-645.0000041
TACGTAG302.163515E-645.0000041
CGGACCA302.163515E-645.00000419
ACAACGG253.888254E-545.02
TAATACG253.888254E-545.04
TCGTTAT253.888254E-545.017
GGTACGT253.888254E-545.08
GATCGAA253.888254E-545.09
GCGAAAG351.2104465E-745.01
CGTTGTT351.2104465E-745.01
CGACCCA207.030207E-445.034
GCACGAG253.888254E-545.01
GCGTTAG253.888254E-545.01
CGTTATT4850.045.01
CGTTAGG502.1827873E-1145.02
CGCTAGG253.888254E-545.02
ACAATCG207.030207E-445.01
CGAATGG207.030207E-445.02
Produced by FastQC (version 0.11.5)