##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562099
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.582335140251093	31.0	31.0	34.0	30.0	34.0
2	31.694020092545976	31.0	31.0	34.0	30.0	34.0
3	31.698709657907237	31.0	31.0	34.0	30.0	34.0
4	35.50741951150954	37.0	35.0	37.0	33.0	37.0
5	32.93498476247067	37.0	35.0	37.0	28.0	37.0
6	33.95629239689094	37.0	35.0	37.0	26.0	37.0
7	35.31002012101071	37.0	35.0	37.0	32.0	37.0
8	35.250694272717084	37.0	35.0	37.0	33.0	37.0
9	37.33242364779158	39.0	37.0	39.0	34.0	39.0
10	36.60616368290995	39.0	35.0	39.0	32.0	39.0
11	36.47622038110724	39.0	35.0	39.0	32.0	39.0
12	36.91647378842517	39.0	37.0	39.0	33.0	39.0
13	36.95018493183585	39.0	37.0	39.0	33.0	39.0
14	37.94205824952544	40.0	37.0	41.0	33.0	41.0
15	38.04785633847418	40.0	37.0	41.0	33.0	41.0
16	38.06280210425565	40.0	37.0	41.0	33.0	41.0
17	37.953716338225114	40.0	37.0	41.0	33.0	41.0
18	37.79183560191354	39.0	37.0	40.0	33.0	41.0
19	37.49080144245053	39.0	36.0	40.0	33.0	41.0
20	37.151551595003724	39.0	35.0	40.0	33.0	41.0
21	37.10942022668605	39.0	35.0	40.0	33.0	41.0
22	37.107103908742054	39.0	35.0	40.0	33.0	41.0
23	36.80110087368951	38.0	35.0	40.0	32.0	41.0
24	36.50752981236401	38.0	35.0	40.0	31.0	41.0
25	36.34408173649126	38.0	35.0	40.0	31.0	41.0
26	36.62294008706651	38.0	35.0	40.0	32.0	41.0
27	36.50789985394032	38.0	35.0	40.0	31.0	41.0
28	36.573423898637074	38.0	35.0	40.0	31.0	41.0
29	36.62598581388688	39.0	35.0	40.0	32.0	41.0
30	36.26340377762636	38.0	35.0	40.0	31.0	41.0
31	36.06998055502678	38.0	35.0	40.0	30.0	41.0
32	35.63099916562741	38.0	35.0	40.0	27.0	41.0
33	35.24863769549492	38.0	35.0	40.0	24.0	41.0
34	34.9442749408912	38.0	35.0	40.0	21.0	41.0
35	34.59324069247588	38.0	35.0	40.0	18.0	41.0
36	34.39224051279223	38.0	34.0	40.0	18.0	41.0
37	34.35359607471282	38.0	34.0	40.0	17.0	41.0
38	34.25799191957289	38.0	34.0	40.0	16.0	41.0
39	34.13044499278597	38.0	34.0	40.0	15.0	41.0
40	34.01951969315014	38.0	34.0	40.0	15.0	41.0
41	33.90084486896436	38.0	33.0	40.0	15.0	41.0
42	33.94819951645529	38.0	34.0	40.0	15.0	41.0
43	33.842810608095725	38.0	33.0	40.0	15.0	41.0
44	33.75971136757048	38.0	33.0	40.0	14.0	41.0
45	33.774909757889624	38.0	33.0	40.0	14.0	41.0
46	33.614124913938646	38.0	33.0	40.0	12.0	41.0
47	33.49237589819587	38.0	33.0	40.0	12.0	41.0
48	33.43561721333786	38.0	33.0	40.0	12.0	41.0
49	33.3924753468695	38.0	33.0	40.0	10.0	41.0
50	33.27002538698699	38.0	33.0	40.0	10.0	41.0
51	32.30528252140637	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	9.0
11	8.0
12	21.0
13	34.0
14	29.0
15	57.0
16	104.0
17	192.0
18	371.0
19	750.0
20	1336.0
21	2258.0
22	3560.0
23	5384.0
24	7376.0
25	10559.0
26	13583.0
27	13985.0
28	13045.0
29	11992.0
30	12110.0
31	13917.0
32	16751.0
33	21521.0
34	31518.0
35	44556.0
36	58726.0
37	77781.0
38	115239.0
39	85287.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.70919001812848	21.23077963134608	22.064796414866418	20.995233935659023
2	38.77057244364427	24.699741504610397	23.009647766674554	13.520038285070779
3	24.811643500522152	24.626266903161188	38.229386638296816	12.332702958019851
4	21.223485542582356	23.01213843113046	41.514928864844094	14.24944716144309
5	18.97655039414765	31.229552089578526	35.467951375113635	14.325946141160188
6	19.47023567022891	32.42916283430499	37.05059073223756	11.050010763228542
7	61.54004899492794	6.960695535839773	26.970871679188186	4.528383790044103
8	59.80209891851791	8.167600369329959	25.610790981659814	6.419509730492315
9	55.645891560027685	8.050361235298409	27.23915182201	9.064595382663908
10	28.076726697610205	21.742789081638644	36.436286134648874	13.74419808610227
11	18.455289904447437	22.814664320697954	39.69656590742912	19.03347986742549
12	15.917302823879778	19.98811597245325	45.292021512224714	18.802559691442255
13	18.38964310557393	21.77338867352548	46.54251297369324	13.294455247207344
14	15.31900964065049	25.453523311729786	42.45640002917636	16.771067018443368
15	13.072608206027764	24.456723815555623	45.815950570984825	16.65471740743179
16	13.471292423576632	24.784957809923164	41.267819369897474	20.475930396602735
17	12.971914200167586	26.29661323005378	40.32261220888135	20.40886036089728
18	13.636387896082363	24.102159939797083	44.465298817468096	17.796153346652456
19	13.60667782721549	27.105011750599097	41.185271633644604	18.10303878854081
20	14.129717362955635	27.510456343099705	42.7791189808201	15.580707313124556
21	13.73975047100244	28.359950827167456	42.192033787642394	15.708264914187714
22	12.266166636126377	24.89116685850713	40.18082223949874	22.661844265867757
23	12.328433247524012	26.04879211669119	40.449102382320554	21.173672253464247
24	14.4757418177225	25.021037219422205	39.8129155184407	20.690305444414598
25	12.435353914523954	28.487152619022627	37.86699495996257	21.21049850649085
26	13.352807957317129	26.526465978413054	41.202172571023965	18.918553493245852
27	15.34907551872535	25.613993264531693	39.21319909837946	19.82373211836349
28	11.682461630424534	24.939556910793296	40.12229162478496	23.255689833997213
29	15.901825123332367	22.87888788273952	39.190783118276315	22.028503875651797
30	15.881899807685123	27.237728585178054	35.82536172453607	21.055009882600753
31	13.82977020062302	27.835665959199357	34.60155595366653	23.733007886511096
32	15.190918325775352	28.662744463163964	34.565975032867875	21.58036217819281
33	15.512747754399136	27.217625364926818	33.18383416444434	24.085792716229705
34	16.093606286437087	24.935109295693465	35.56811166716184	23.403172750707615
35	17.366513728008766	26.580193168819015	32.530212649373155	23.523080453799064
36	19.08080249208769	27.36902218292507	30.41937452299328	23.130800801993956
37	14.662363747311415	26.0247749951521	36.13171345261244	23.181147804924045
38	14.997536021234694	29.585713548680925	32.58927697789891	22.82747345218547
39	18.870519250167675	26.487504870138533	35.56526519349794	19.076710686195845
40	18.09556679517309	27.397842728771977	35.25660070556966	19.24998977048527
41	19.030099679949615	28.00681018824086	30.68729885660711	22.275791275202412
42	21.469349705301024	24.677681333715235	32.1866788590622	21.666290101921547
43	20.916600100694005	23.013561667962403	32.817350680218254	23.252487551125338
44	17.660234229201617	23.31190768885908	32.357645183499706	26.670212898439598
45	18.96623192711604	22.161576519438746	33.39376159715638	25.47842995628884
46	20.632664352720784	25.025484834522032	33.877840024621996	20.464010788135187
47	15.716626430575397	25.9141183314683	37.59426720204092	20.77498803591538
48	17.28503341937986	24.209614320609003	34.984228756856	23.521123503155138
49	19.463297390673173	21.558124102693654	37.16782986626911	21.810748640364064
50	18.03579084823136	20.417221877284963	35.96839702614664	25.578590248337036
51	17.178468561587906	21.15303531940103	32.2233272074848	29.44516891152626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3946.0
1	5418.0
2	6890.0
3	16537.5
4	26185.0
5	17427.0
6	8669.0
7	8467.5
8	8266.0
9	8621.5
10	8977.0
11	8990.5
12	9004.0
13	8973.5
14	8943.0
15	8537.5
16	8132.0
17	7712.5
18	7293.0
19	6927.5
20	6562.0
21	6424.0
22	6286.0
23	6133.5
24	5981.0
25	5959.5
26	6231.0
27	6524.0
28	7133.5
29	7743.0
30	8156.5
31	8570.0
32	9171.5
33	9773.0
34	10363.0
35	10953.0
36	11583.5
37	12214.0
38	13031.5
39	13849.0
40	16208.5
41	18568.0
42	23310.0
43	28052.0
44	32109.0
45	36166.0
46	49851.5
47	63537.0
48	66874.5
49	70212.0
50	67211.5
51	64211.0
52	50991.5
53	37772.0
54	29017.0
55	20262.0
56	15862.0
57	11462.0
58	9410.0
59	7358.0
60	6014.5
61	4671.0
62	4099.5
63	3528.0
64	2779.5
65	2031.0
66	1714.5
67	1398.0
68	1088.5
69	779.0
70	661.5
71	544.0
72	439.0
73	334.0
74	263.0
75	158.5
76	125.0
77	113.0
78	101.0
79	83.0
80	65.0
81	45.0
82	25.0
83	13.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.68385628193659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45635150711368	35.6927732559932
2	10.533766940276012	9.835137238945205
3	4.270606837262627	5.981051874822726
4	2.2689262565960635	4.23688909108972
5	1.4142343367130596	3.30109562620462
6	0.995006017394829	2.787043074943338
7	0.7497180528815597	2.449981088288725
8	0.567831851644433	2.1206864443579714
9	0.46939208540306326	1.9721729389351599
>10	2.1493310110829413	16.098355880515985
>50	0.07105394266926239	2.2611069014104945
>100	0.04435964371362597	3.6463968208864177
>500	0.0043181954057512	1.5163234304676192
>1k	0.004710758624455856	5.234376338048844
>5k	0.0	0.0
>10k+	3.925632187046546E-4	2.8666099950899806
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15642	2.7827838156623654	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC	4588	0.816226323121016	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	3100	0.551504272379065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3098	0.5511484631710784	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	3094	0.5504368447551055	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	2918	0.519125634452294	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	2479	0.44102551329925865	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2180	0.3878320367052779	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	1782	0.3170260043159657	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1509	0.26845804742580937	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	1483	0.2638325277219849	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	1253	0.22291446880353818	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1078	0.19178116310472	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	991	0.17630346255730753	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTATAGT	930	0.16545128171371945	No Hit
ACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	826	0.1469492028984218	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	796	0.1416120647786244	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	772	0.1373423542827865	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT	761	0.13538540363886076	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT	723	0.1286250286871174	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGT	714	0.12702388725117816	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC	671	0.11937398927946857	No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTG	568	0.10104981506816416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7790460399324674E-4	0.0	0.0	0.3381966521911621	0.0
2	1.7790460399324674E-4	0.0	0.0	1.7329687474982165	0.0
3	1.7790460399324674E-4	0.0	0.0	2.3536779108306543	0.0
4	3.558092079864935E-4	0.0	0.0	3.3673783443841745	0.0
5	3.558092079864935E-4	0.0	0.0	6.2592176822944	0.0
6	3.558092079864935E-4	0.0	0.0	7.71839124424701	0.0
7	3.558092079864935E-4	0.0	0.0	9.313839732858447	0.0
8	3.558092079864935E-4	0.0	0.0	11.67676868309675	0.0
9	3.558092079864935E-4	0.0	0.0	12.480007970126259	0.0
10	3.558092079864935E-4	0.0	0.0	15.472719218500655	0.0
11	3.558092079864935E-4	0.0	0.0	19.48606917998431	0.0
12	3.558092079864935E-4	0.0	0.0	22.54300399040027	0.0
13	3.558092079864935E-4	0.0	0.0	23.53606748989057	0.0
14	3.558092079864935E-4	0.0	0.0	23.9304819969436	0.0
15	3.558092079864935E-4	0.0	0.0	24.780866004031317	0.0
16	3.558092079864935E-4	0.0	0.0	26.57450022149123	0.0
17	3.558092079864935E-4	0.0	0.0	28.925331658657996	0.0
18	3.558092079864935E-4	0.0	0.0	31.286481562856366	0.0
19	3.558092079864935E-4	0.0	0.0	32.93494562345779	0.0
20	3.558092079864935E-4	0.0	0.0	34.266917393555225	0.0
21	3.558092079864935E-4	0.0	0.0	35.90666412856098	0.0
22	3.558092079864935E-4	0.0	0.0	37.539472584011	0.0
23	3.558092079864935E-4	0.0	0.0	39.20056787149595	0.0
24	3.558092079864935E-4	0.0	0.0	40.3213668766534	0.0
25	3.558092079864935E-4	0.0	0.0	41.196479623696185	0.0
26	3.558092079864935E-4	0.0	0.0	41.93389420724819	0.0
27	3.558092079864935E-4	0.0	0.0	42.6106433208385	0.0
28	5.337138119797402E-4	0.0	0.0	43.28134367789304	0.0
29	5.337138119797402E-4	0.0	0.0	43.93122919628037	0.0
30	5.337138119797402E-4	0.0	0.0	44.571507866052066	0.0
31	5.337138119797402E-4	0.0	0.0	45.12923879957089	0.0
32	5.337138119797402E-4	0.0	0.0	45.70120210140918	0.0
33	5.337138119797402E-4	0.0	0.0	46.19257461763853	0.0
34	5.337138119797402E-4	0.0	0.0	46.643206979553426	0.0
35	5.337138119797402E-4	0.0	0.0	47.10415780849993	0.0
36	5.337138119797402E-4	0.0	0.0	47.54607284481915	0.0
37	5.337138119797402E-4	0.0	0.0	47.968240470095125	0.0
38	5.337138119797402E-4	0.0	0.0	48.39485571047093	0.0
39	5.337138119797402E-4	0.0	0.0	48.780908701136276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAT	30	2.163515E-6	45.000004	4
CGATGTG	30	2.163515E-6	45.000004	10
GACGAAG	30	2.163515E-6	45.000004	1
TACGTAG	30	2.163515E-6	45.000004	1
CGGACCA	30	2.163515E-6	45.000004	19
ACAACGG	25	3.888254E-5	45.0	2
TAATACG	25	3.888254E-5	45.0	4
TCGTTAT	25	3.888254E-5	45.0	17
GGTACGT	25	3.888254E-5	45.0	8
GATCGAA	25	3.888254E-5	45.0	9
GCGAAAG	35	1.2104465E-7	45.0	1
CGTTGTT	35	1.2104465E-7	45.0	1
CGACCCA	20	7.030207E-4	45.0	34
GCACGAG	25	3.888254E-5	45.0	1
GCGTTAG	25	3.888254E-5	45.0	1
CGTTATT	485	0.0	45.0	1
CGTTAGG	50	2.1827873E-11	45.0	2
CGCTAGG	25	3.888254E-5	45.0	2
ACAATCG	20	7.030207E-4	45.0	1
CGAATGG	20	7.030207E-4	45.0	2
>>END_MODULE