FastQCFastQC Report
Sat 14 Jan 2017
SRR2935216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439093
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112922.5716647726108137No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG39710.9043642235244015No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC39150.8916106610672454No Hit
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC32350.736745974087494No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC32110.7312801616058557No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC22930.5222128341831913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21700.49420054521479506No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC19430.4425030688259663No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT13960.3179280926819603No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.30312485054418997No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCCTAAT10000.2277421867349286No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC9460.21544410865124244No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCT9340.2127112024104233No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.2038292571277611No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT8200.18674859312264144No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.17399503066548544No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG7410.16875696037058208No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.15987501508791988No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTC6650.15144855417872752No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6650.15144855417872752No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC6570.14962661668484808No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.13414014798687296No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTA5750.13095175737258394No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC5260.11979239022257243No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG253.886853E-545.0000042
CGAAAGG253.886853E-545.0000042
TCCGAGT253.886853E-545.00000412
TTGAGTA253.886853E-545.0000047
CGTTAGG453.8380676E-1045.0000042
CTCGGCG253.886853E-545.00000416
GCGTAAG253.886853E-545.0000041
GGCTACG453.8380676E-1045.0000041
GCGACAA207.028518E-445.011
ATTAGCG207.028518E-445.01
GGCACTA207.028518E-445.08
TTACTAC207.028518E-445.01
TCGATAG207.028518E-445.01
CGTTATT2350.045.01
CTGCCTA207.028518E-445.023
GCCCTAC207.028518E-445.026
CGAATGC351.209628E-745.045
GCCTACG207.028518E-445.025
CGTAAGG351.209628E-745.02
TACTACC207.028518E-445.02
Produced by FastQC (version 0.11.5)