##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439093
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599055325409424	31.0	31.0	34.0	30.0	34.0
2	31.706340114736513	31.0	31.0	34.0	30.0	34.0
3	31.71168522385918	31.0	31.0	34.0	30.0	34.0
4	35.543636541689345	37.0	35.0	37.0	33.0	37.0
5	33.00895026793868	37.0	35.0	37.0	28.0	37.0
6	33.99505116228225	37.0	35.0	37.0	27.0	37.0
7	35.28940566121528	37.0	35.0	37.0	32.0	37.0
8	35.14191071139827	37.0	35.0	37.0	32.0	37.0
9	37.19134670787282	39.0	37.0	39.0	34.0	39.0
10	36.76270630595341	39.0	37.0	39.0	32.0	39.0
11	36.569405570118406	39.0	35.0	39.0	32.0	39.0
12	36.842529942404	39.0	37.0	39.0	33.0	39.0
13	36.74059937188705	39.0	35.0	39.0	33.0	39.0
14	37.54745805558276	39.0	37.0	41.0	32.0	41.0
15	37.6963877811762	40.0	37.0	41.0	32.0	41.0
16	37.76220527314259	40.0	37.0	41.0	33.0	41.0
17	37.68807519136037	39.0	36.0	41.0	33.0	41.0
18	37.587479190057685	39.0	36.0	40.0	33.0	41.0
19	37.37482720061581	39.0	36.0	40.0	33.0	41.0
20	37.0499415841291	39.0	35.0	40.0	32.0	41.0
21	37.03410894730729	39.0	35.0	40.0	32.0	41.0
22	37.07610916138495	39.0	35.0	40.0	32.0	41.0
23	36.74178363125807	38.0	35.0	40.0	32.0	41.0
24	36.45687360080894	38.0	35.0	40.0	31.0	41.0
25	36.28720794911329	38.0	35.0	40.0	31.0	41.0
26	36.60146028290134	38.0	35.0	40.0	32.0	41.0
27	36.44811008146338	38.0	35.0	40.0	31.0	41.0
28	36.497776097546534	38.0	35.0	40.0	31.0	41.0
29	36.59203858863612	38.0	35.0	40.0	31.0	41.0
30	36.33570564777849	38.0	35.0	40.0	31.0	41.0
31	36.14850157028238	38.0	35.0	40.0	30.0	41.0
32	35.81831867053221	38.0	35.0	40.0	29.0	41.0
33	35.62273140314239	38.0	35.0	40.0	27.0	41.0
34	35.442815075621795	38.0	35.0	40.0	25.0	41.0
35	35.18829040772684	38.0	35.0	40.0	24.0	41.0
36	34.99593024712305	38.0	35.0	40.0	23.0	41.0
37	34.954672472574146	38.0	35.0	40.0	23.0	41.0
38	34.85828742430419	38.0	35.0	40.0	23.0	41.0
39	34.78113748112587	38.0	35.0	40.0	22.0	41.0
40	34.687865668548575	38.0	34.0	40.0	21.0	41.0
41	34.41472307688804	38.0	34.0	40.0	20.0	41.0
42	34.47541864707477	38.0	34.0	40.0	21.0	41.0
43	34.43637452658093	38.0	34.0	40.0	20.0	41.0
44	34.41266428751996	38.0	34.0	40.0	20.0	41.0
45	34.410329930105924	38.0	34.0	40.0	20.0	41.0
46	34.28015705101197	38.0	34.0	40.0	20.0	41.0
47	34.118455543586435	38.0	33.0	40.0	20.0	41.0
48	34.077054291459895	38.0	33.0	40.0	20.0	41.0
49	34.09625979006725	38.0	34.0	40.0	20.0	41.0
50	34.0399254827565	38.0	34.0	40.0	19.0	41.0
51	33.111773587827635	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	7.0
11	9.0
12	24.0
13	13.0
14	19.0
15	50.0
16	85.0
17	133.0
18	251.0
19	514.0
20	907.0
21	1627.0
22	2445.0
23	3582.0
24	4950.0
25	6505.0
26	7995.0
27	8583.0
28	8568.0
29	8700.0
30	9647.0
31	11679.0
32	14407.0
33	19905.0
34	28944.0
35	36782.0
36	46366.0
37	59767.0
38	87857.0
39	68749.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44678462193658	21.28911187379439	20.24240878356066	18.02169472070837
2	36.170014097241356	26.353870364592467	22.756682525114268	14.719433013051905
3	25.35499313357307	27.597798188538647	33.97161876868909	13.07558990919919
4	23.196224945512682	22.739374118922413	38.13930078593828	15.925100149626617
5	21.370415834458758	30.465755546091604	32.21299360272197	15.950835016727662
6	20.285452056853558	33.25400313828734	34.53095357931007	11.92959122554903
7	61.7308861676228	7.563318021466979	25.461120992591546	5.24467481831867
8	58.21090292944775	10.775621565363146	23.646471248687636	7.36700425650147
9	53.035917220269965	9.671527444072213	25.353626680452663	11.93892865520516
10	30.45436843675486	23.976697419453284	31.67711623733469	13.891817906457174
11	23.103078391138094	21.92861193414607	36.17320248785565	18.795107186860186
12	19.980277526628758	19.51704991880991	40.97081939361365	19.531853160947684
13	20.827478461282688	21.190044022564695	43.987720141291256	13.994757374861361
14	15.918495626211303	26.11041396697283	39.99858799844224	17.972502408373625
15	13.288984338169818	23.487507202346656	44.8460804430952	18.37742801638833
16	15.018003019861395	25.61826310143865	37.5134652567907	21.850268621909255
17	14.96881070752665	25.971946717437994	37.38684060096608	21.672401974069274
18	15.594418494487499	23.505954319472185	41.29603523627113	19.603591949769182
19	14.978603621556253	25.614391484264154	38.34244681650584	21.06455807767375
20	15.547048119646636	28.180590444393328	40.23452890389963	16.037832532060406
21	14.821689254895887	28.39922294365886	40.57636992618876	16.202717875256496
22	13.316313400578009	23.61253766286413	39.137267048210745	23.933881888347116
23	13.577305946576237	26.207887622895377	39.24339490722922	20.971411523299164
24	16.393110343366896	24.758991830887762	36.26133871412207	22.586559111623277
25	13.447720642324063	29.613544283329503	34.19230094763524	22.746434126711197
26	13.281013361634095	25.21948653246579	37.846652075983904	23.652848029916214
27	18.07430316584414	24.751931823098978	36.58359390835199	20.590171102704893
28	11.757645874564158	24.902469408530767	38.67996073724701	24.659923979658068
29	15.687565048862087	22.606828166242686	34.79331257842871	26.912294206466512
30	19.839305113039835	24.877873252363393	33.75526369129091	21.52755794330586
31	16.044664797662453	25.98834415488291	34.258573923975106	23.708417123479535
32	16.748160412486648	27.496680657628335	30.01687569603706	25.73828323384795
33	17.585568433110982	24.285971309039315	30.667307381352018	27.461152876497692
34	14.445003678036317	23.12129776607689	35.54918889620195	26.884509659684852
35	16.121413914592125	26.53834153584776	29.124126324036137	28.216118225523978
36	18.207987829457544	28.085394210338126	30.554802741104957	23.15181521909937
37	17.356459793255645	25.119279970302415	35.83090598119305	21.693354255248888
38	15.672306322350845	27.014094963937023	29.893667173013462	27.419931540698666
39	17.52772191768031	23.221504328240258	31.905086166256353	27.345687587823083
40	18.556661117348717	23.665829334560105	33.4487227079457	24.32878684014548
41	16.44366910882205	29.025513957179918	29.93374979787881	24.59706713611923
42	18.258091110539226	26.772005019437795	32.72450255412863	22.245401315894355
43	20.144935127638107	23.848023083948046	31.779144736991938	24.22789705142191
44	19.273821263377005	20.331455978574013	32.04674180640548	28.347980951643503
45	19.05906038128597	19.089350092121716	31.68235430762959	30.169235218962726
46	21.743685278517308	22.188010284837155	32.05494052512793	24.013363911517605
47	14.807796981505057	24.274356457515832	38.29575966822518	22.622086892753927
48	16.36373160127809	24.378890121227165	34.071369846478994	25.186008431015754
49	19.075230076544152	20.606796282336543	36.981687250764644	23.336286390354662
50	17.676437565618215	19.96205815168996	34.97436761688299	27.387136665808836
51	16.979090989835864	19.993714315646116	31.220037668557687	31.80715702596033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3544.0
1	4991.5
2	6439.0
3	12331.5
4	18224.0
5	11612.0
6	5000.0
7	4914.0
8	4828.0
9	5076.0
10	5324.0
11	5370.5
12	5417.0
13	5160.5
14	4904.0
15	4834.0
16	4764.0
17	4540.0
18	4316.0
19	4063.5
20	3811.0
21	3752.0
22	3693.0
23	3857.5
24	4022.0
25	4381.5
26	4689.5
27	4638.0
28	4962.0
29	5286.0
30	5331.5
31	5377.0
32	5907.0
33	6437.0
34	6848.0
35	7259.0
36	7691.5
37	8124.0
38	8930.0
39	9736.0
40	10718.0
41	11700.0
42	14494.0
43	17288.0
44	21255.5
45	25223.0
46	37974.0
47	50725.0
48	52054.0
49	53383.0
50	52396.5
51	51410.0
52	41856.5
53	32303.0
54	26135.5
55	19968.0
56	16804.5
57	13641.0
58	11963.5
59	10286.0
60	9036.5
61	7787.0
62	6827.5
63	5868.0
64	5186.5
65	4505.0
66	3752.0
67	2999.0
68	2569.5
69	2140.0
70	1831.0
71	1522.0
72	1278.0
73	1034.0
74	826.5
75	480.5
76	342.0
77	288.0
78	234.0
79	178.5
80	123.0
81	92.0
82	61.0
83	47.5
84	34.0
85	19.0
86	4.0
87	4.0
88	4.0
89	3.0
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	439093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.073899352306896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95807227485025	31.117044891826882
2	10.710371099867725	9.012541513633824
3	4.512182749677616	5.695353686074541
4	2.4656575974311217	4.149593183662715
5	1.6716116414175277	3.516560997857279
6	1.2107839154585867	3.0565440357837983
7	0.9966717247044676	2.9353706082754125
8	0.7634597679086892	2.5697383547620616
9	0.6423035237849687	2.4321792432024743
>10	2.9269240649687567	18.83823760531676
>50	0.07978464259649325	2.3171532333688614
>100	0.04897126338681287	3.8872334840140286
>500	0.007703344802420001	2.4259615078901198
>1k	0.004952150230127144	5.432310982216019
>5k	0.0	0.0
>10k+	5.502389144585716E-4	2.6141766721152053
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11292	2.5716647726108137	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	3971	0.9043642235244015	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	3915	0.8916106610672454	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	3235	0.736745974087494	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	3211	0.7312801616058557	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC	2293	0.5222128341831913	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2170	0.49420054521479506	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1943	0.4425030688259663	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	1396	0.3179280926819603	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1331	0.30312485054418997	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCCTAAT	1000	0.2277421867349286	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	946	0.21544410865124244	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCT	934	0.2127112024104233	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.2038292571277611	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	820	0.18674859312264144	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.17399503066548544	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	741	0.16875696037058208	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	702	0.15987501508791988	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTC	665	0.15144855417872752	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	665	0.15144855417872752	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	657	0.14962661668484808	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	589	0.13414014798687296	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTA	575	0.13095175737258394	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	526	0.11979239022257243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.277421867349286E-4	0.0	0.0	0.3393358582350436	0.0
2	2.277421867349286E-4	0.0	0.0	2.002992532333697	0.0
3	2.277421867349286E-4	0.0	0.0	2.6486416317272194	0.0
4	2.277421867349286E-4	0.0	0.0	3.727911854664046	0.0
5	2.277421867349286E-4	0.0	0.0	7.205079561732936	0.0
6	2.277421867349286E-4	0.0	0.0	8.703623150448767	0.0
7	2.277421867349286E-4	0.0	0.0	10.39916373069031	0.0
8	2.277421867349286E-4	0.0	0.0	12.766316019613157	0.0
9	2.277421867349286E-4	0.0	0.0	13.603040813677286	0.0
10	2.277421867349286E-4	0.0	0.0	16.864992154281666	0.0
11	4.554843734698572E-4	0.0	0.0	20.704269938259092	0.0
12	4.554843734698572E-4	0.0	0.0	24.21127187179026	0.0
13	4.554843734698572E-4	0.0	0.0	25.304434368117917	0.0
14	4.554843734698572E-4	0.0	0.0	25.787247803995964	0.0
15	4.554843734698572E-4	0.0	0.0	26.84055541764501	0.0
16	4.554843734698572E-4	0.0	0.0	28.585971536781503	0.0
17	4.554843734698572E-4	0.0	0.0	30.56391242857436	0.0
18	4.554843734698572E-4	0.0	0.0	32.66346764808366	0.0
19	4.554843734698572E-4	0.0	0.0	34.31550947065884	0.0
20	4.554843734698572E-4	0.0	0.0	35.73616523151132	0.0
21	4.554843734698572E-4	0.0	0.0	37.33696506207113	0.0
22	4.554843734698572E-4	0.0	0.0	38.88675064280232	0.0
23	4.554843734698572E-4	0.0	0.0	40.19148563060673	0.0
24	4.554843734698572E-4	0.0	0.0	41.07398660420458	0.0
25	4.554843734698572E-4	0.0	0.0	41.82439710949617	0.0
26	4.554843734698572E-4	0.0	0.0	42.474828794811124	0.0
27	4.554843734698572E-4	0.0	0.0	43.152361800347535	0.0
28	4.554843734698572E-4	0.0	0.0	43.71397403283587	0.0
29	4.554843734698572E-4	0.0	0.0	44.21477910146598	0.0
30	4.554843734698572E-4	0.0	0.0	44.7841345683033	0.0
31	4.554843734698572E-4	0.0	0.0	45.20614084032312	0.0
32	4.554843734698572E-4	0.0	0.0	45.58578706561025	0.0
33	4.554843734698572E-4	0.0	0.0	45.980919759595345	0.0
34	4.554843734698572E-4	0.0	0.0	46.37013115672534	0.0
35	4.554843734698572E-4	0.0	0.0	46.757292874174716	0.0
36	4.554843734698572E-4	0.0	0.0	47.10915455268018	0.0
37	4.554843734698572E-4	0.0	0.0	47.452134285902986	0.0
38	4.554843734698572E-4	0.0	0.0	47.84066245647278	0.0
39	4.554843734698572E-4	0.0	0.0	48.32711976733858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	25	3.886853E-5	45.000004	2
CGAAAGG	25	3.886853E-5	45.000004	2
TCCGAGT	25	3.886853E-5	45.000004	12
TTGAGTA	25	3.886853E-5	45.000004	7
CGTTAGG	45	3.8380676E-10	45.000004	2
CTCGGCG	25	3.886853E-5	45.000004	16
GCGTAAG	25	3.886853E-5	45.000004	1
GGCTACG	45	3.8380676E-10	45.000004	1
GCGACAA	20	7.028518E-4	45.0	11
ATTAGCG	20	7.028518E-4	45.0	1
GGCACTA	20	7.028518E-4	45.0	8
TTACTAC	20	7.028518E-4	45.0	1
TCGATAG	20	7.028518E-4	45.0	1
CGTTATT	235	0.0	45.0	1
CTGCCTA	20	7.028518E-4	45.0	23
GCCCTAC	20	7.028518E-4	45.0	26
CGAATGC	35	1.209628E-7	45.0	45
GCCTACG	20	7.028518E-4	45.0	25
CGTAAGG	35	1.209628E-7	45.0	2
TACTACC	20	7.028518E-4	45.0	2
>>END_MODULE