FastQCFastQC Report
Sat 14 Jan 2017
SRR2935215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441208
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153373.4761382386538773No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC79921.811390545955649No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC75221.7048648256604595No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG75211.704638175191746No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC30850.6992166959801273RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT25910.5872513644358216No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC20640.46780656742398147No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT18210.41273050352668134No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT14170.32116371416656087RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13770.3120976954180341No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13280.30099182245108885No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA12930.2930590560461279No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC12760.289205998078004No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC12180.2760602708926402RNA PCR Primer, Index 1 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.25430182589617595No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.22733042011930882No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.216904498558503No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT9160.20761182934126307Illumina Paired End PCR Primer 2 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8440.19129299559391488No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.16998785153487697No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.16341498794219508No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT6890.15616217294337364No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG6450.1461895523199942No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4880.11060542873202661No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC4770.10811227357618175RNA PCR Primer, Index 23 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC4750.10765897263875543No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG4610.10448586607677103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG600.045.0000044
AGTCCGG302.1624492E-645.0000042
GAACTAT302.1624492E-645.0000049
GCGATCG302.1624492E-645.00000433
CGATCGC302.1624492E-645.00000434
TAGCGAG207.0285564E-445.01
TCGGGCG207.0285564E-445.05
CGTTCGG207.0285564E-445.02
AGCGGGT253.8868857E-545.04
AATGCGG253.8868857E-545.02
TACGCAG207.0285564E-445.01
CGCTCAC253.8868857E-545.034
CTACGAA207.0285564E-445.011
GCCCTAC207.0285564E-445.026
CGAATAT207.0285564E-445.014
CGAGCAC551.8189894E-1245.034
CTCGAGG253.8868857E-545.02
TTACGAG253.8868857E-545.01
ATACGTC351.2096461E-745.06
ATTGCGG253.8868857E-545.02
Produced by FastQC (version 0.11.5)