##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935215.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 441208 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64435821653279 31.0 31.0 34.0 30.0 34.0 2 31.760251400699897 31.0 31.0 34.0 30.0 34.0 3 31.765790738155246 31.0 31.0 34.0 30.0 34.0 4 35.580873420246235 37.0 35.0 37.0 33.0 37.0 5 33.03951877572483 37.0 35.0 37.0 28.0 37.0 6 34.025992275752024 37.0 35.0 37.0 28.0 37.0 7 35.27691927616906 37.0 35.0 37.0 32.0 37.0 8 35.03310456746024 37.0 35.0 37.0 32.0 37.0 9 37.07308571014125 39.0 37.0 39.0 33.0 39.0 10 36.75314364200105 39.0 35.0 39.0 32.0 39.0 11 36.58328724773803 39.0 35.0 39.0 32.0 39.0 12 36.894457942739024 39.0 37.0 39.0 33.0 39.0 13 36.81146760711501 39.0 37.0 39.0 32.0 39.0 14 37.837672934307626 40.0 37.0 41.0 33.0 41.0 15 37.88689461659807 40.0 37.0 41.0 33.0 41.0 16 38.01242497869485 40.0 37.0 41.0 33.0 41.0 17 37.716648383528856 40.0 37.0 41.0 32.0 41.0 18 37.670762996137874 39.0 37.0 40.0 33.0 41.0 19 37.45278190785299 39.0 36.0 40.0 33.0 41.0 20 36.821233975811865 39.0 35.0 40.0 31.0 41.0 21 37.01106280937789 39.0 35.0 40.0 32.0 41.0 22 37.02311608130405 39.0 35.0 40.0 32.0 41.0 23 36.557369766640676 38.0 35.0 40.0 31.0 41.0 24 36.39577478196225 38.0 35.0 40.0 30.0 41.0 25 36.30526191728165 38.0 35.0 40.0 30.0 41.0 26 36.464490671066706 38.0 35.0 40.0 31.0 41.0 27 36.251117386810755 38.0 35.0 40.0 30.0 41.0 28 36.288129408351615 38.0 35.0 40.0 30.0 41.0 29 36.379940980217945 38.0 35.0 40.0 30.0 41.0 30 36.119845968341465 38.0 35.0 40.0 30.0 41.0 31 35.922730322206306 38.0 35.0 40.0 29.0 41.0 32 35.50203985421842 38.0 35.0 40.0 26.0 41.0 33 35.28379358488513 38.0 35.0 40.0 24.0 41.0 34 34.85702888433573 38.0 35.0 40.0 21.0 41.0 35 34.65790284854309 38.0 35.0 40.0 20.0 41.0 36 34.47299006364345 38.0 34.0 40.0 18.0 41.0 37 34.427023082083736 38.0 34.0 40.0 18.0 41.0 38 34.24810746858625 38.0 34.0 40.0 18.0 41.0 39 34.13166125727548 38.0 34.0 40.0 16.0 41.0 40 34.13976627803666 38.0 34.0 40.0 18.0 41.0 41 33.863948523145545 38.0 33.0 40.0 16.0 41.0 42 34.002289169734006 38.0 34.0 40.0 15.0 41.0 43 33.89327256078766 38.0 33.0 40.0 15.0 41.0 44 33.968640641148845 38.0 33.0 40.0 15.0 41.0 45 33.91120287936756 38.0 33.0 40.0 16.0 41.0 46 33.762021540860545 38.0 33.0 40.0 15.0 41.0 47 33.56203196678211 38.0 33.0 40.0 15.0 41.0 48 33.547093434389225 37.0 33.0 40.0 15.0 41.0 49 33.58525457380646 37.0 33.0 40.0 15.0 41.0 50 33.48518159235553 37.0 33.0 40.0 15.0 41.0 51 32.53372558974452 36.0 31.0 40.0 12.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 8.0 10 5.0 11 13.0 12 7.0 13 16.0 14 30.0 15 37.0 16 56.0 17 115.0 18 279.0 19 617.0 20 1435.0 21 2864.0 22 4446.0 23 5439.0 24 6136.0 25 7441.0 26 8717.0 27 9180.0 28 9284.0 29 9205.0 30 9464.0 31 11510.0 32 14358.0 33 17917.0 34 25602.0 35 36119.0 36 44369.0 37 56033.0 38 82714.0 39 77767.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06289097205853 17.650178600569348 18.955911950825914 19.33101847654621 2 34.609753222969665 32.859331653097854 20.11885550579319 12.41205961813929 3 23.473509093216805 32.73195408968106 32.90602164965277 10.888515167449366 4 20.92641112581821 21.333248717158344 44.77185363819333 12.968486518830122 5 26.623723957861145 29.142717267139307 30.360283585066455 13.873275189933093 6 17.984714692389982 39.029437362876465 32.374072999582964 10.611774945150588 7 56.887907744193214 7.447961052383456 31.51665427644104 4.147476926982286 8 54.38206016210042 16.773494587586807 22.970118402204857 5.874326848107922 9 49.66546390817936 9.517506482203405 24.033562401407046 16.783467208210187 10 31.368198219433918 25.826594259396927 30.850981849830468 11.954225671338687 11 24.379431016663343 21.348660949030844 37.900491378216174 16.371416656089643 12 18.237429965005166 19.004868452067956 40.42265779405631 22.335043788870557 13 21.9055411506591 20.183677539845153 44.46360900074342 13.447172308752334 14 15.519664194665555 29.741074504542077 37.38576816376856 17.353493137023808 15 12.4363112182916 23.49549418868198 44.67552718899023 19.39266740403619 16 13.065266268970642 25.113098583887872 37.206487643016445 24.615147504125037 17 13.267665137531504 28.13389603089699 40.04233830755562 18.556100524015882 18 12.644376348570288 24.59157585537887 40.88887780819931 21.875169987851535 19 13.881434606806767 25.406610940871428 38.16499247520444 22.54696197711737 20 17.492883175282405 26.328171746659173 42.04456854816776 14.134376529890663 21 15.581993073561677 30.77210748671828 38.80074703994488 14.845152399775163 22 12.398913890953926 23.46172326884372 42.408342550452396 21.731020289749956 23 16.254011713296222 26.566154738808002 38.76289641166978 18.416937136225997 24 15.665400446048123 26.02332686623996 37.041939402730684 21.269333284981233 25 12.965766713205563 33.22333230585121 34.5156932784537 19.295207702489527 26 14.089953038022882 24.95648311000707 40.903610088665666 20.04995376330438 27 19.444343710902796 25.75655926456456 36.05646316476582 18.742633859766823 28 12.356076952367138 29.151103334481697 38.64775797356349 19.845061739587678 29 20.09687041032801 21.81329440989284 38.05620025022212 20.033634929557035 30 16.683967652445105 29.672852713459413 34.86292179652227 18.78025783757321 31 17.641339232289532 28.523961487552356 30.97518630668528 22.859512973472828 32 23.429765552755164 28.012411379666734 31.500335442693693 17.057487624884406 33 21.634467190078148 25.962357890156117 30.19142898587514 22.21174593389059 34 20.91371869957027 27.70280683940454 30.99218509183877 20.391289369186417 35 20.53611901869413 24.258626316839223 32.63268118438469 22.572573480081957 36 19.872939747239396 32.71903501296441 28.896121557179384 18.511903682616815 37 18.387019274355858 27.8569291581295 33.188428133669376 20.567623433845263 38 20.111149389856937 29.14725027651357 29.102373483708366 21.639226849921126 39 24.651411579119145 28.241781654004463 29.50173160958097 17.605075157295424 40 21.166660622654167 24.34475349495023 34.27272397599318 20.21586190640242 41 16.93826947834128 32.05064278072927 30.117767583543365 20.893320157386086 42 17.567451179489037 26.353103298217622 36.415930808144914 19.663514714148427 43 21.805588293956593 28.0015321571685 29.852133234211532 20.34074631466338 44 20.646724447426156 24.584323040380045 31.96723540824282 22.80171710395097 45 20.1827709379703 23.16775761092274 31.34054686225091 25.30892458885605 46 25.237076390273977 26.74724846330982 30.414679697557617 17.60099544885859 47 17.02031694801545 26.148891225907057 38.684701999963735 18.14608982611376 48 18.192326521731246 27.98883973092056 32.37543290239524 21.443400844952947 49 18.822414824753857 22.573026781019383 39.006545665536436 19.59801272869032 50 20.059473082990337 22.789251328171748 34.21900781490816 22.932267773929755 51 18.346222189987486 21.777936936773585 31.972901669960653 27.902939203278272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2512.0 1 4038.0 2 5564.0 3 14736.5 4 23909.0 5 14986.5 6 6064.0 7 5712.5 8 5361.0 9 5412.5 10 5464.0 11 5409.0 12 5354.0 13 5118.5 14 4883.0 15 4665.0 16 4447.0 17 4335.0 18 4223.0 19 4357.5 20 4492.0 21 4454.0 22 4416.0 23 4854.5 24 5293.0 25 6330.5 26 7200.5 27 7033.0 28 7478.0 29 7923.0 30 8318.5 31 8714.0 32 9163.5 33 9613.0 34 9816.5 35 10020.0 36 10226.5 37 10433.0 38 11051.0 39 11669.0 40 12741.5 41 13814.0 42 16321.5 43 18829.0 44 21688.0 45 24547.0 46 42140.0 47 59733.0 48 50871.5 49 42010.0 50 40773.0 51 39536.0 52 31693.5 53 23851.0 54 20279.0 55 16707.0 56 14569.5 57 12432.0 58 11071.0 59 9710.0 60 8880.0 61 8050.0 62 7091.0 63 6132.0 64 5183.0 65 4234.0 66 3360.0 67 2486.0 68 2020.5 69 1555.0 70 1345.0 71 1135.0 72 916.5 73 698.0 74 565.0 75 357.0 76 282.0 77 212.0 78 142.0 79 111.0 80 80.0 81 54.5 82 29.0 83 22.0 84 15.0 85 10.5 86 6.0 87 4.5 88 3.0 89 1.5 90 0.0 91 0.5 92 1.0 93 1.5 94 2.0 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 441208.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.86883657802325 #Duplication Level Percentage of deduplicated Percentage of total 1 75.10084721307244 31.443850988352224 2 9.896136995893364 8.286794852695783 3 4.198659275127999 5.273789371114071 4 2.5365501372005452 4.248096126656483 5 1.7843862728378044 3.735508862475702 6 1.3432143785896433 3.374329398385249 7 1.0715514395697818 3.1405228475804923 8 0.8646360889051541 2.896104568466486 9 0.6690035093870523 2.5209358744185484 >10 2.409934314491776 15.124905224983962 >50 0.07163193277613844 2.0551069062560714 >100 0.040775100195647875 3.252919553009713 >500 0.003857104072561285 1.2739448065049386 >1k 0.006612178410105061 4.520635364625909 >5k 0.0016530446025262652 5.3142554541545195 >10k+ 5.510148675087551E-4 3.538299800319856 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15337 3.4761382386538773 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC 7992 1.811390545955649 No Hit GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC 7522 1.7048648256604595 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG 7521 1.704638175191746 No Hit GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC 3085 0.6992166959801273 RNA PCR Primer, Index 23 (95% over 23bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT 2591 0.5872513644358216 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC 2064 0.46780656742398147 No Hit GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT 1821 0.41273050352668134 No Hit GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT 1417 0.32116371416656087 RNA PCR Primer, Index 23 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1377 0.3120976954180341 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 1328 0.30099182245108885 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA 1293 0.2930590560461279 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC 1276 0.289205998078004 No Hit CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC 1218 0.2760602708926402 RNA PCR Primer, Index 1 (95% over 22bp) CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.25430182589617595 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.22733042011930882 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 0.216904498558503 No Hit CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT 916 0.20761182934126307 Illumina Paired End PCR Primer 2 (95% over 23bp) GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 844 0.19129299559391488 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.16998785153487697 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 721 0.16341498794219508 No Hit GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT 689 0.15616217294337364 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG 645 0.1461895523199942 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 488 0.11060542873202661 No Hit TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC 477 0.10811227357618175 RNA PCR Primer, Index 23 (95% over 23bp) CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC 475 0.10765897263875543 No Hit GAATAATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG 461 0.10448586607677103 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.22098420699534008 0.0 2 0.0 0.0 0.0 1.4682417363239106 0.0 3 0.0 0.0 0.0 1.881198890319305 0.0 4 0.0 0.0 0.0 2.70394009174811 0.0 5 0.0 0.0 0.0 5.847355442331055 0.0 6 0.0 0.0 0.0 6.864562745915759 0.0 7 0.0 0.0 0.0 8.200440608511178 0.0 8 0.0 0.0 0.0 9.784954035284946 0.0 9 0.0 0.0 0.0 10.288571376765606 0.0 10 0.0 0.0 0.0 13.572963318888144 0.0 11 0.0 0.0 0.0 15.989283965839242 0.0 12 0.0 0.0 0.0 19.757574658664396 0.0 13 0.0 0.0 0.0 20.500534895106163 0.0 14 0.0 0.0 0.0 20.86498884879694 0.0 15 0.0 0.0 0.0 21.771364073180905 0.0 16 0.0 0.0 0.0 23.00048956501242 0.0 17 0.0 0.0 0.0 24.502502221174595 0.0 18 0.0 0.0 0.0 25.90342876829069 0.0 19 0.0 0.0 0.0 27.642971115664267 0.0 20 0.0 0.0 0.0 28.63434026581567 0.0 21 0.0 0.0 0.0 29.573126507225616 0.0 22 0.0 0.0 0.0 30.754882051096082 0.0 23 0.0 0.0 0.0 31.740584939529654 0.0 24 0.0 0.0 0.0 32.45702707113198 0.0 25 0.0 0.0 0.0 33.01617377744737 0.0 26 0.0 0.0 0.0 33.52681728345814 0.0 27 0.0 0.0 0.0 34.01570234447245 0.0 28 0.0 0.0 0.0 34.475802795960185 0.0 29 0.0 0.0 0.0 34.9236641221374 0.0 30 0.0 0.0 0.0 35.43521423002303 0.0 31 0.0 0.0 0.0 35.84137186995703 0.0 32 0.0 0.0 0.0 36.25432902395242 0.0 33 2.266504687131693E-4 0.0 0.0 36.657766858261866 0.0 34 2.266504687131693E-4 0.0 0.0 37.038992946637414 0.0 35 2.266504687131693E-4 0.0 0.0 37.421125636887815 0.0 36 2.266504687131693E-4 0.0 0.0 37.7513553698029 0.0 37 2.266504687131693E-4 0.0 0.0 38.08203840365542 0.0 38 2.266504687131693E-4 0.0 0.0 38.40546862250911 0.0 39 2.266504687131693E-4 0.0 0.0 38.73025874417508 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCCG 60 0.0 45.000004 4 AGTCCGG 30 2.1624492E-6 45.000004 2 GAACTAT 30 2.1624492E-6 45.000004 9 GCGATCG 30 2.1624492E-6 45.000004 33 CGATCGC 30 2.1624492E-6 45.000004 34 TAGCGAG 20 7.0285564E-4 45.0 1 TCGGGCG 20 7.0285564E-4 45.0 5 CGTTCGG 20 7.0285564E-4 45.0 2 AGCGGGT 25 3.8868857E-5 45.0 4 AATGCGG 25 3.8868857E-5 45.0 2 TACGCAG 20 7.0285564E-4 45.0 1 CGCTCAC 25 3.8868857E-5 45.0 34 CTACGAA 20 7.0285564E-4 45.0 11 GCCCTAC 20 7.0285564E-4 45.0 26 CGAATAT 20 7.0285564E-4 45.0 14 CGAGCAC 55 1.8189894E-12 45.0 34 CTCGAGG 25 3.8868857E-5 45.0 2 TTACGAG 25 3.8868857E-5 45.0 1 ATACGTC 35 1.2096461E-7 45.0 6 ATTGCGG 25 3.8868857E-5 45.0 2 >>END_MODULE