##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935215.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441208
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64435821653279	31.0	31.0	34.0	30.0	34.0
2	31.760251400699897	31.0	31.0	34.0	30.0	34.0
3	31.765790738155246	31.0	31.0	34.0	30.0	34.0
4	35.580873420246235	37.0	35.0	37.0	33.0	37.0
5	33.03951877572483	37.0	35.0	37.0	28.0	37.0
6	34.025992275752024	37.0	35.0	37.0	28.0	37.0
7	35.27691927616906	37.0	35.0	37.0	32.0	37.0
8	35.03310456746024	37.0	35.0	37.0	32.0	37.0
9	37.07308571014125	39.0	37.0	39.0	33.0	39.0
10	36.75314364200105	39.0	35.0	39.0	32.0	39.0
11	36.58328724773803	39.0	35.0	39.0	32.0	39.0
12	36.894457942739024	39.0	37.0	39.0	33.0	39.0
13	36.81146760711501	39.0	37.0	39.0	32.0	39.0
14	37.837672934307626	40.0	37.0	41.0	33.0	41.0
15	37.88689461659807	40.0	37.0	41.0	33.0	41.0
16	38.01242497869485	40.0	37.0	41.0	33.0	41.0
17	37.716648383528856	40.0	37.0	41.0	32.0	41.0
18	37.670762996137874	39.0	37.0	40.0	33.0	41.0
19	37.45278190785299	39.0	36.0	40.0	33.0	41.0
20	36.821233975811865	39.0	35.0	40.0	31.0	41.0
21	37.01106280937789	39.0	35.0	40.0	32.0	41.0
22	37.02311608130405	39.0	35.0	40.0	32.0	41.0
23	36.557369766640676	38.0	35.0	40.0	31.0	41.0
24	36.39577478196225	38.0	35.0	40.0	30.0	41.0
25	36.30526191728165	38.0	35.0	40.0	30.0	41.0
26	36.464490671066706	38.0	35.0	40.0	31.0	41.0
27	36.251117386810755	38.0	35.0	40.0	30.0	41.0
28	36.288129408351615	38.0	35.0	40.0	30.0	41.0
29	36.379940980217945	38.0	35.0	40.0	30.0	41.0
30	36.119845968341465	38.0	35.0	40.0	30.0	41.0
31	35.922730322206306	38.0	35.0	40.0	29.0	41.0
32	35.50203985421842	38.0	35.0	40.0	26.0	41.0
33	35.28379358488513	38.0	35.0	40.0	24.0	41.0
34	34.85702888433573	38.0	35.0	40.0	21.0	41.0
35	34.65790284854309	38.0	35.0	40.0	20.0	41.0
36	34.47299006364345	38.0	34.0	40.0	18.0	41.0
37	34.427023082083736	38.0	34.0	40.0	18.0	41.0
38	34.24810746858625	38.0	34.0	40.0	18.0	41.0
39	34.13166125727548	38.0	34.0	40.0	16.0	41.0
40	34.13976627803666	38.0	34.0	40.0	18.0	41.0
41	33.863948523145545	38.0	33.0	40.0	16.0	41.0
42	34.002289169734006	38.0	34.0	40.0	15.0	41.0
43	33.89327256078766	38.0	33.0	40.0	15.0	41.0
44	33.968640641148845	38.0	33.0	40.0	15.0	41.0
45	33.91120287936756	38.0	33.0	40.0	16.0	41.0
46	33.762021540860545	38.0	33.0	40.0	15.0	41.0
47	33.56203196678211	38.0	33.0	40.0	15.0	41.0
48	33.547093434389225	37.0	33.0	40.0	15.0	41.0
49	33.58525457380646	37.0	33.0	40.0	15.0	41.0
50	33.48518159235553	37.0	33.0	40.0	15.0	41.0
51	32.53372558974452	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	5.0
11	13.0
12	7.0
13	16.0
14	30.0
15	37.0
16	56.0
17	115.0
18	279.0
19	617.0
20	1435.0
21	2864.0
22	4446.0
23	5439.0
24	6136.0
25	7441.0
26	8717.0
27	9180.0
28	9284.0
29	9205.0
30	9464.0
31	11510.0
32	14358.0
33	17917.0
34	25602.0
35	36119.0
36	44369.0
37	56033.0
38	82714.0
39	77767.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06289097205853	17.650178600569348	18.955911950825914	19.33101847654621
2	34.609753222969665	32.859331653097854	20.11885550579319	12.41205961813929
3	23.473509093216805	32.73195408968106	32.90602164965277	10.888515167449366
4	20.92641112581821	21.333248717158344	44.77185363819333	12.968486518830122
5	26.623723957861145	29.142717267139307	30.360283585066455	13.873275189933093
6	17.984714692389982	39.029437362876465	32.374072999582964	10.611774945150588
7	56.887907744193214	7.447961052383456	31.51665427644104	4.147476926982286
8	54.38206016210042	16.773494587586807	22.970118402204857	5.874326848107922
9	49.66546390817936	9.517506482203405	24.033562401407046	16.783467208210187
10	31.368198219433918	25.826594259396927	30.850981849830468	11.954225671338687
11	24.379431016663343	21.348660949030844	37.900491378216174	16.371416656089643
12	18.237429965005166	19.004868452067956	40.42265779405631	22.335043788870557
13	21.9055411506591	20.183677539845153	44.46360900074342	13.447172308752334
14	15.519664194665555	29.741074504542077	37.38576816376856	17.353493137023808
15	12.4363112182916	23.49549418868198	44.67552718899023	19.39266740403619
16	13.065266268970642	25.113098583887872	37.206487643016445	24.615147504125037
17	13.267665137531504	28.13389603089699	40.04233830755562	18.556100524015882
18	12.644376348570288	24.59157585537887	40.88887780819931	21.875169987851535
19	13.881434606806767	25.406610940871428	38.16499247520444	22.54696197711737
20	17.492883175282405	26.328171746659173	42.04456854816776	14.134376529890663
21	15.581993073561677	30.77210748671828	38.80074703994488	14.845152399775163
22	12.398913890953926	23.46172326884372	42.408342550452396	21.731020289749956
23	16.254011713296222	26.566154738808002	38.76289641166978	18.416937136225997
24	15.665400446048123	26.02332686623996	37.041939402730684	21.269333284981233
25	12.965766713205563	33.22333230585121	34.5156932784537	19.295207702489527
26	14.089953038022882	24.95648311000707	40.903610088665666	20.04995376330438
27	19.444343710902796	25.75655926456456	36.05646316476582	18.742633859766823
28	12.356076952367138	29.151103334481697	38.64775797356349	19.845061739587678
29	20.09687041032801	21.81329440989284	38.05620025022212	20.033634929557035
30	16.683967652445105	29.672852713459413	34.86292179652227	18.78025783757321
31	17.641339232289532	28.523961487552356	30.97518630668528	22.859512973472828
32	23.429765552755164	28.012411379666734	31.500335442693693	17.057487624884406
33	21.634467190078148	25.962357890156117	30.19142898587514	22.21174593389059
34	20.91371869957027	27.70280683940454	30.99218509183877	20.391289369186417
35	20.53611901869413	24.258626316839223	32.63268118438469	22.572573480081957
36	19.872939747239396	32.71903501296441	28.896121557179384	18.511903682616815
37	18.387019274355858	27.8569291581295	33.188428133669376	20.567623433845263
38	20.111149389856937	29.14725027651357	29.102373483708366	21.639226849921126
39	24.651411579119145	28.241781654004463	29.50173160958097	17.605075157295424
40	21.166660622654167	24.34475349495023	34.27272397599318	20.21586190640242
41	16.93826947834128	32.05064278072927	30.117767583543365	20.893320157386086
42	17.567451179489037	26.353103298217622	36.415930808144914	19.663514714148427
43	21.805588293956593	28.0015321571685	29.852133234211532	20.34074631466338
44	20.646724447426156	24.584323040380045	31.96723540824282	22.80171710395097
45	20.1827709379703	23.16775761092274	31.34054686225091	25.30892458885605
46	25.237076390273977	26.74724846330982	30.414679697557617	17.60099544885859
47	17.02031694801545	26.148891225907057	38.684701999963735	18.14608982611376
48	18.192326521731246	27.98883973092056	32.37543290239524	21.443400844952947
49	18.822414824753857	22.573026781019383	39.006545665536436	19.59801272869032
50	20.059473082990337	22.789251328171748	34.21900781490816	22.932267773929755
51	18.346222189987486	21.777936936773585	31.972901669960653	27.902939203278272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2512.0
1	4038.0
2	5564.0
3	14736.5
4	23909.0
5	14986.5
6	6064.0
7	5712.5
8	5361.0
9	5412.5
10	5464.0
11	5409.0
12	5354.0
13	5118.5
14	4883.0
15	4665.0
16	4447.0
17	4335.0
18	4223.0
19	4357.5
20	4492.0
21	4454.0
22	4416.0
23	4854.5
24	5293.0
25	6330.5
26	7200.5
27	7033.0
28	7478.0
29	7923.0
30	8318.5
31	8714.0
32	9163.5
33	9613.0
34	9816.5
35	10020.0
36	10226.5
37	10433.0
38	11051.0
39	11669.0
40	12741.5
41	13814.0
42	16321.5
43	18829.0
44	21688.0
45	24547.0
46	42140.0
47	59733.0
48	50871.5
49	42010.0
50	40773.0
51	39536.0
52	31693.5
53	23851.0
54	20279.0
55	16707.0
56	14569.5
57	12432.0
58	11071.0
59	9710.0
60	8880.0
61	8050.0
62	7091.0
63	6132.0
64	5183.0
65	4234.0
66	3360.0
67	2486.0
68	2020.5
69	1555.0
70	1345.0
71	1135.0
72	916.5
73	698.0
74	565.0
75	357.0
76	282.0
77	212.0
78	142.0
79	111.0
80	80.0
81	54.5
82	29.0
83	22.0
84	15.0
85	10.5
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.86883657802325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10084721307244	31.443850988352224
2	9.896136995893364	8.286794852695783
3	4.198659275127999	5.273789371114071
4	2.5365501372005452	4.248096126656483
5	1.7843862728378044	3.735508862475702
6	1.3432143785896433	3.374329398385249
7	1.0715514395697818	3.1405228475804923
8	0.8646360889051541	2.896104568466486
9	0.6690035093870523	2.5209358744185484
>10	2.409934314491776	15.124905224983962
>50	0.07163193277613844	2.0551069062560714
>100	0.040775100195647875	3.252919553009713
>500	0.003857104072561285	1.2739448065049386
>1k	0.006612178410105061	4.520635364625909
>5k	0.0016530446025262652	5.3142554541545195
>10k+	5.510148675087551E-4	3.538299800319856
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15337	3.4761382386538773	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	7992	1.811390545955649	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	7522	1.7048648256604595	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	7521	1.704638175191746	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	3085	0.6992166959801273	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	2591	0.5872513644358216	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	2064	0.46780656742398147	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	1821	0.41273050352668134	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	1417	0.32116371416656087	RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1377	0.3120976954180341	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1328	0.30099182245108885	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA	1293	0.2930590560461279	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	1276	0.289205998078004	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1218	0.2760602708926402	RNA PCR Primer, Index 1 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1122	0.25430182589617595	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1003	0.22733042011930882	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.216904498558503	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	916	0.20761182934126307	Illumina Paired End PCR Primer 2 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	844	0.19129299559391488	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.16998785153487697	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	721	0.16341498794219508	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	689	0.15616217294337364	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	645	0.1461895523199942	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	488	0.11060542873202661	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	477	0.10811227357618175	RNA PCR Primer, Index 23 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	475	0.10765897263875543	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	461	0.10448586607677103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22098420699534008	0.0
2	0.0	0.0	0.0	1.4682417363239106	0.0
3	0.0	0.0	0.0	1.881198890319305	0.0
4	0.0	0.0	0.0	2.70394009174811	0.0
5	0.0	0.0	0.0	5.847355442331055	0.0
6	0.0	0.0	0.0	6.864562745915759	0.0
7	0.0	0.0	0.0	8.200440608511178	0.0
8	0.0	0.0	0.0	9.784954035284946	0.0
9	0.0	0.0	0.0	10.288571376765606	0.0
10	0.0	0.0	0.0	13.572963318888144	0.0
11	0.0	0.0	0.0	15.989283965839242	0.0
12	0.0	0.0	0.0	19.757574658664396	0.0
13	0.0	0.0	0.0	20.500534895106163	0.0
14	0.0	0.0	0.0	20.86498884879694	0.0
15	0.0	0.0	0.0	21.771364073180905	0.0
16	0.0	0.0	0.0	23.00048956501242	0.0
17	0.0	0.0	0.0	24.502502221174595	0.0
18	0.0	0.0	0.0	25.90342876829069	0.0
19	0.0	0.0	0.0	27.642971115664267	0.0
20	0.0	0.0	0.0	28.63434026581567	0.0
21	0.0	0.0	0.0	29.573126507225616	0.0
22	0.0	0.0	0.0	30.754882051096082	0.0
23	0.0	0.0	0.0	31.740584939529654	0.0
24	0.0	0.0	0.0	32.45702707113198	0.0
25	0.0	0.0	0.0	33.01617377744737	0.0
26	0.0	0.0	0.0	33.52681728345814	0.0
27	0.0	0.0	0.0	34.01570234447245	0.0
28	0.0	0.0	0.0	34.475802795960185	0.0
29	0.0	0.0	0.0	34.9236641221374	0.0
30	0.0	0.0	0.0	35.43521423002303	0.0
31	0.0	0.0	0.0	35.84137186995703	0.0
32	0.0	0.0	0.0	36.25432902395242	0.0
33	2.266504687131693E-4	0.0	0.0	36.657766858261866	0.0
34	2.266504687131693E-4	0.0	0.0	37.038992946637414	0.0
35	2.266504687131693E-4	0.0	0.0	37.421125636887815	0.0
36	2.266504687131693E-4	0.0	0.0	37.7513553698029	0.0
37	2.266504687131693E-4	0.0	0.0	38.08203840365542	0.0
38	2.266504687131693E-4	0.0	0.0	38.40546862250911	0.0
39	2.266504687131693E-4	0.0	0.0	38.73025874417508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	60	0.0	45.000004	4
AGTCCGG	30	2.1624492E-6	45.000004	2
GAACTAT	30	2.1624492E-6	45.000004	9
GCGATCG	30	2.1624492E-6	45.000004	33
CGATCGC	30	2.1624492E-6	45.000004	34
TAGCGAG	20	7.0285564E-4	45.0	1
TCGGGCG	20	7.0285564E-4	45.0	5
CGTTCGG	20	7.0285564E-4	45.0	2
AGCGGGT	25	3.8868857E-5	45.0	4
AATGCGG	25	3.8868857E-5	45.0	2
TACGCAG	20	7.0285564E-4	45.0	1
CGCTCAC	25	3.8868857E-5	45.0	34
CTACGAA	20	7.0285564E-4	45.0	11
GCCCTAC	20	7.0285564E-4	45.0	26
CGAATAT	20	7.0285564E-4	45.0	14
CGAGCAC	55	1.8189894E-12	45.0	34
CTCGAGG	25	3.8868857E-5	45.0	2
TTACGAG	25	3.8868857E-5	45.0	1
ATACGTC	35	1.2096461E-7	45.0	6
ATTGCGG	25	3.8868857E-5	45.0	2
>>END_MODULE