SRR2935214.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935214.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319035
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6037	1.8922688733211088	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4903	1.5368219787797577	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	3542	1.1102230162834799	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2907	0.9111852931496545	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2317	0.7262526055135017	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	1910	0.5986803955678844	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	1788	0.5604400771075274	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	1774	0.5560518438415848	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	1613	0.5055871612832448	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	1515	0.47486952842164654	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	1442	0.4519880263920886	No Hit
ACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	1028	0.3222216998135001	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	551	0.1727083235381698	No Hit
ACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	537	0.1683200902722272	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	500	0.15672261664080742	No Hit
AGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	479	0.1501402667418935	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	448	0.14042346451016346	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	420	0.13164699797827825	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.13007977181187017	No Hit
GCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	412	0.12913943611202533	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	404	0.1266318742457724	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	385	0.12067641481342173	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	384	0.12036296958014012	No Hit
GAATAAGATTCCATGGTTTTATTTATTTCTTTATTTCTTTCTTTATTTATT	381	0.11942263388029528	No Hit
GGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	381	0.11942263388029528	No Hit
TATAATGGGAAGATATTGTACATAAAGAGAATTTTTTTTGCCTTTAAATAG	370	0.11597473631419751	No Hit
TTTTTTGGGAAATTTATCTCTATATAAAAGTATATGTAATATATGCCTATT	363	0.11378061968122619	No Hit
GACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	359	0.11252683874809974	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	349	0.1093923864152836	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.10719826978231228	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.10594448884918584	No Hit
GGCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	334	0.10469070791605936	No Hit
AAAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	333	0.10437726268277775	No Hit
GGAGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	322	0.10092936511667998	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8844482E-5	45.000004	1
GTCATAC	25	3.8844482E-5	45.000004	39
CGGGTAG	20	7.025616E-4	45.000004	6
TGGAGCG	25	3.8844482E-5	45.000004	1
GTACCGT	20	7.025616E-4	45.000004	16
GGTACAT	20	7.025616E-4	45.000004	7
AATCATA	20	7.025616E-4	45.000004	29
TACCATA	25	3.8844482E-5	45.000004	45
ATCTACG	20	7.025616E-4	45.000004	1
CGTGCGG	20	7.025616E-4	45.000004	2
TTAGCAG	25	3.8844482E-5	45.000004	1
CTGAGTA	20	7.025616E-4	45.000004	21
TGGGTCA	20	7.025616E-4	45.000004	6
GGCGTGC	20	7.025616E-4	45.000004	37
GGCGTCA	25	3.8844482E-5	45.000004	36
AGAAGCG	20	7.025616E-4	45.000004	26
GATCCAA	20	7.025616E-4	45.000004	9
GTAGGTG	20	7.025616E-4	45.000004	4
AGGCGTC	25	3.8844482E-5	45.000004	35
CGCGACG	20	7.025616E-4	45.000004	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content