SRR2935212.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935212.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647533
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21651	3.343613375688961	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	10244	1.582004314838008	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	9440	1.4578407586949238	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	9401	1.4518178996282816	No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	4519	0.6978794903116907	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	4058	0.6266862074983051	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAATCGGT	3575	0.5520954144421983	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2690	0.4154228433145492	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT	2293	0.3541132266618072	No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	2155	0.3328015715029195	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT	2092	0.32307233762603604	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	1890	0.2918770163065048	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTA	1801	0.2781325430518599	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTC	1576	0.2433852792058474	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	1302	0.20107083345559223	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.16833118929846047	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	995	0.1536601223412552	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.1338927900199681	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	845	0.13049527977724687	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	778	0.12014831676532316	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	744	0.11489761911748127	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGGTCGAATGC	719	0.1110368120234799	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTT	715	0.11041908288843966	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.10285190098419693	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGTA	45	3.8380676E-10	45.000004	45
CGAATAC	45	3.8380676E-10	45.000004	45
CGGGTGC	25	3.8889117E-5	45.0	6
CGGAAGC	20	7.031003E-4	45.0	43
GTCTGCG	20	7.031003E-4	45.0	1
CGACGTA	20	7.031003E-4	45.0	45
TGCCACG	20	7.031003E-4	45.0	1
CGACGCG	40	6.8066583E-9	45.0	15
CCGTCGA	50	2.1827873E-11	45.0	41
TTGAGCG	20	7.031003E-4	45.0	1
TAGCACG	25	3.8889117E-5	45.0	1
ACGGACG	50	2.1827873E-11	45.0	43
CCGACGT	25	3.8889117E-5	45.0	44
CGTAAGC	20	7.031003E-4	45.0	43
CGTCGAC	20	7.031003E-4	45.0	9
CGCAATC	40	6.8066583E-9	45.0	20
GACGAAT	40	6.8066583E-9	45.0	20
ACATACG	20	7.031003E-4	45.0	17
CCGCCGT	20	7.031003E-4	45.0	40
TACGTCG	20	7.031003E-4	45.0	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content