FastQCFastQC Report
Sat 14 Jan 2017
SRR2935211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5334869
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54956310.301340107882687No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC400720.7511337204343724No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370520.6945250202019956No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG179810.3370467016153536No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC139310.2611310605752456No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT137440.2576258198654925No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC121930.22855294103754No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT110100.20637807601273805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109350.20497223080829163No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG105070.19694954084158392No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT88910.16665826283644453No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT84530.15844812684247728No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA76350.14311504181264806No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCT72890.13662940926946848No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC68860.1290753343709096No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTC68730.12883165453547218No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC62960.11801601876259755No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA58380.10943099071411126No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC56330.10558834715529096No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG55000.10309531499273927No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGGAAT53380.10005868935113495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT2029950.044.527821
CGTTATT149100.044.381291
CCGATGA60950.042.2313418
CGATGAA63000.041.96428719
GTTATTT161600.041.3103372
ACCACCG73300.041.07094214
GATGAAT69050.040.17740620
GTTTTTT2266250.039.9316062
CCACCGA73150.039.83253515
CGTAAGG4450.039.43822
CGACCAC91500.039.4180312
TAATACG6200.038.8306434
TGAATGA69150.038.75271222
CACCGAT68500.038.62773516
ATCGAAT7800.038.36538343
ATGAATG71650.038.18562321
GCGACCA94050.038.0861211
ACCGATG67550.038.0384917
GACCACC92700.037.69417613
CCAATCG3950.037.59493640
Produced by FastQC (version 0.11.5)