##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5334869
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13313972657998	33.0	31.0	34.0	30.0	34.0
2	32.30885294465525	34.0	31.0	34.0	30.0	34.0
3	32.15364388516382	33.0	31.0	34.0	30.0	34.0
4	35.87160003366531	37.0	35.0	37.0	35.0	37.0
5	33.365390977735345	37.0	35.0	37.0	30.0	37.0
6	34.40427290716979	37.0	35.0	37.0	28.0	37.0
7	35.662490869035395	37.0	35.0	37.0	32.0	37.0
8	35.611328413125044	37.0	35.0	37.0	33.0	37.0
9	37.74638758702416	39.0	38.0	39.0	35.0	39.0
10	37.39994102947983	39.0	37.0	39.0	34.0	39.0
11	37.2413020825816	39.0	37.0	39.0	34.0	39.0
12	37.464048133140665	39.0	37.0	39.0	35.0	39.0
13	37.45442202985678	39.0	37.0	39.0	35.0	39.0
14	38.71944222060561	40.0	38.0	41.0	35.0	41.0
15	38.778769450571325	40.0	38.0	41.0	34.0	41.0
16	38.75933073520643	40.0	38.0	41.0	34.0	41.0
17	38.60298350343748	40.0	38.0	41.0	34.0	41.0
18	38.038240114237105	39.0	38.0	40.0	34.0	41.0
19	37.33479378781372	37.0	37.0	40.0	34.0	41.0
20	36.495017778318456	36.0	35.0	40.0	33.0	41.0
21	36.43677492362043	36.0	35.0	40.0	33.0	41.0
22	36.37070038645747	35.0	35.0	40.0	33.0	41.0
23	36.16405876133041	35.0	35.0	40.0	33.0	41.0
24	35.83545781536529	35.0	35.0	40.0	32.0	41.0
25	35.687560837951224	35.0	35.0	40.0	31.0	41.0
26	35.84877773006235	35.0	35.0	40.0	32.0	41.0
27	35.75605642800226	35.0	35.0	40.0	32.0	41.0
28	35.68040208672415	35.0	35.0	40.0	32.0	41.0
29	35.610534391753575	35.0	35.0	40.0	31.0	41.0
30	35.1763068596436	35.0	35.0	40.0	30.0	41.0
31	34.67666572506279	35.0	35.0	40.0	25.0	41.0
32	33.71917511001676	35.0	33.0	40.0	20.0	41.0
33	32.62043379134521	35.0	33.0	40.0	15.0	41.0
34	31.49620637357731	35.0	30.0	40.0	8.0	41.0
35	30.707027857666233	35.0	24.0	40.0	7.0	41.0
36	30.238558060188545	35.0	22.0	40.0	7.0	41.0
37	30.02154448403513	35.0	21.0	40.0	7.0	41.0
38	29.85141734501822	35.0	21.0	40.0	7.0	41.0
39	29.612820108609977	35.0	20.0	40.0	7.0	41.0
40	29.446596720556773	35.0	20.0	40.0	7.0	41.0
41	29.231512338915913	35.0	18.0	40.0	7.0	41.0
42	29.13022644042431	35.0	18.0	40.0	7.0	41.0
43	29.01122782958682	35.0	18.0	40.0	7.0	41.0
44	28.915546567310276	35.0	18.0	40.0	7.0	41.0
45	28.881543483073344	35.0	18.0	40.0	7.0	41.0
46	28.773311959487664	35.0	18.0	40.0	7.0	41.0
47	28.638206298973788	35.0	18.0	39.0	7.0	41.0
48	28.57309710885122	35.0	18.0	39.0	7.0	41.0
49	28.489298987472793	35.0	18.0	39.0	7.0	41.0
50	28.391241846800735	35.0	17.0	39.0	7.0	41.0
51	27.75985483429865	34.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	41.0
9	59.0
10	73.0
11	102.0
12	164.0
13	229.0
14	428.0
15	685.0
16	1320.0
17	2584.0
18	5189.0
19	10332.0
20	18761.0
21	31584.0
22	47436.0
23	69673.0
24	112161.0
25	196954.0
26	293467.0
27	312598.0
28	266774.0
29	210675.0
30	175787.0
31	162695.0
32	167766.0
33	192761.0
34	269837.0
35	401673.0
36	488501.0
37	445977.0
38	660277.0
39	788059.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.43938042339934	11.83622315749459	13.345369867563758	47.37902655154231
2	59.44354022563628	17.664313781650495	13.950445643557508	8.941700349155715
3	17.611472746566037	17.705814332085758	55.81861522747794	8.86409769387027
4	15.14668870032235	15.22481620448412	59.33176615958142	10.296728935612103
5	15.884963623286719	22.97747142432176	51.29850423693628	9.839060715455243
6	14.94814586824906	22.180544639427886	54.565294855412574	8.306014636910485
7	45.949750593688435	3.9881579097818523	47.675678634283244	2.386412862246477
8	46.69213058464978	5.875252044614404	45.00041144402984	2.4322059267059792
9	42.897548187218845	5.425175388561556	45.59435292600437	6.082923498215233
10	22.649872002480286	19.080805920445282	49.999447034219585	8.269875042854848
11	17.124731647581225	16.555870444054015	55.28827418255256	11.031123725812199
12	14.043700042119124	15.333966026157345	57.39679830938679	13.225535622336743
13	15.252408259696724	15.56883589831353	58.43240012079022	10.746355721199526
14	12.285887432287465	19.16645001030016	56.9901716424527	11.557490914959674
15	11.71196518602425	17.973974618683233	58.012483530523426	12.301576664769089
16	13.795858904876576	18.361669236864113	55.51392171016758	12.328550148091734
17	13.74504228688652	19.394253167228662	55.740881359973415	11.119823185911407
18	13.35453597829675	18.393253892457338	55.77878294668529	12.473427182560622
19	13.025774391086268	19.196535097675312	54.827006998672324	12.9506835125661
20	14.488621932422333	19.94751886128788	55.492121737197294	10.071737469092493
21	13.628600814752904	20.443632261635667	55.906846072509	10.020920851102435
22	13.063301085743623	17.517149905649042	57.24841603420815	12.171132974399184
23	13.635873720610572	18.834295649996278	56.14934499797465	11.380485631418503
24	12.81291068253035	20.35393933759198	55.344058120265	11.489091859612673
25	12.702411249460857	21.906142400122665	54.21705762596982	11.174388724446654
26	12.729497200399859	19.906993030194368	55.29474107049302	12.068768698912757
27	14.271465709842172	19.368066957220506	54.572136635407546	11.78833069752978
28	12.100540800533247	20.636345522261184	54.924104040792756	12.339009636412815
29	14.092960858082925	19.017749076875177	55.4121010281602	11.477189036881692
30	14.26569237220258	20.550851389228114	54.08333363012288	11.10012260844643
31	14.13296184030011	19.70880634557287	53.34537736540484	12.812854448722172
32	15.648406736885198	21.72994313449871	51.17100344919435	11.450646679421745
33	16.40746942427265	21.795418031820464	49.02212219269114	12.774990351215749
34	16.60363169179974	23.09226337141549	46.90072052378419	13.403384413000582
35	16.71028848131041	25.423210954195874	44.46868329850274	13.397817265990975
36	16.869467647659203	26.999369619010327	42.92251599804981	13.20864673528066
37	16.772445583949672	26.372212701005402	43.20717153504613	13.648170179998797
38	16.936985706678083	26.084558027572935	41.91915490333502	15.05930136241396
39	17.730088592615864	26.480031655885085	41.11519889241892	14.674680859080139
40	17.824973771614637	26.0149780622542	41.56267004869286	14.597378117438309
41	16.691000285105407	27.161191774343475	40.625477401600676	15.52233053895044
42	16.625731578413642	26.800020769019824	41.43503804873184	15.139209603834697
43	17.358701778806566	26.230878396451722	40.71378322504264	15.696636599699074
44	17.297032035838182	25.563683007024164	40.58778200551878	16.55150295161887
45	16.68382110226137	25.39128889575358	40.477170104832936	17.447719897152115
46	17.396265962669375	25.939737226912225	40.467310443799086	16.19668636661931
47	15.986446902445028	26.185422734841286	42.142196931171135	15.685933431542557
48	16.206208624804095	25.789742915899154	41.158217755674976	16.845830703621775
49	16.30553627464892	25.434701395666888	41.94374782211147	16.316014507572728
50	15.958086318520662	25.276984308330718	41.754108676333004	17.01082069681561
51	16.00588505547184	25.033998023194197	40.737851294942764	18.222265626391202
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13338.0
1	14763.0
2	16188.0
3	394511.0
4	772834.0
5	481748.5
6	190663.0
7	182682.5
8	174702.0
9	176854.0
10	179006.0
11	177579.0
12	176152.0
13	171266.0
14	166380.0
15	158748.5
16	151117.0
17	140533.5
18	129950.0
19	120194.5
20	110439.0
21	102330.0
22	94221.0
23	89026.0
24	83831.0
25	81758.0
26	78501.5
27	77318.0
28	80209.0
29	83100.0
30	87516.5
31	91933.0
32	97161.5
33	102390.0
34	112226.0
35	122062.0
36	125763.5
37	129465.0
38	134510.5
39	139556.0
40	148544.5
41	157533.0
42	163714.5
43	169896.0
44	185809.0
45	201722.0
46	265838.5
47	329955.0
48	281101.5
49	232248.0
50	226738.5
51	221229.0
52	202048.0
53	182867.0
54	172410.0
55	161953.0
56	147959.5
57	133966.0
58	125511.0
59	117056.0
60	109181.5
61	101307.0
62	88314.5
63	75322.0
64	65382.0
65	55442.0
66	46683.0
67	37924.0
68	31458.5
69	24993.0
70	22076.5
71	19160.0
72	15002.5
73	10845.0
74	9237.5
75	5831.5
76	4033.0
77	3519.0
78	3005.0
79	2133.5
80	1262.0
81	933.0
82	604.0
83	454.0
84	304.0
85	221.5
86	139.0
87	112.5
88	86.0
89	53.5
90	21.0
91	18.0
92	15.0
93	15.0
94	15.0
95	9.0
96	3.0
97	2.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5334869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.479110392650476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53982524544367	22.937025741697674
2	7.196663936255843	4.0990917339887325
3	2.79771735702861	2.390295043747563
4	1.5704217844797914	1.7889686145289254
5	1.0583846912723962	1.5070927230318931
6	0.8124282122802261	1.388233964617934
7	0.6180099009678993	1.2320260535391054
8	0.506803516329504	1.1546650631145106
9	0.43473062925668876	1.1142667423500883
>10	3.5989040732973563	21.6287503664199
>50	0.664423374832067	13.124071283344707
>100	0.1871699170965318	8.053244220580645
>500	0.008559765023678183	1.7005513601715163
>1k	0.00438259967688822	2.2412431793003793
>5k	8.902155593679196E-4	1.6578030606556275
>10k+	6.847811995137843E-4	13.982670848910761
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	549563	10.301340107882687	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	40072	0.7511337204343724	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37052	0.6945250202019956	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	17981	0.3370467016153536	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	13931	0.2611310605752456	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	13744	0.2576258198654925	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	12193	0.22855294103754	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT	11010	0.20637807601273805	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10935	0.20497223080829163	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	10507	0.19694954084158392	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT	8891	0.16665826283644453	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT	8453	0.15844812684247728	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	7635	0.14311504181264806	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCT	7289	0.13662940926946848	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	6886	0.1290753343709096	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTC	6873	0.12883165453547218	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	6296	0.11801601876259755	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	5838	0.10943099071411126	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	5633	0.10558834715529096	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	5500	0.10309531499273927	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGGAAT	5338	0.10005868935113495	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.497841090381038E-5	0.0	0.0	0.03235318430499418	0.0
2	7.497841090381038E-5	0.0	0.0	0.22686592679220427	0.0
3	7.497841090381038E-5	0.0	0.0	0.3422014673649906	0.0
4	7.497841090381038E-5	0.0	0.0	0.7187243023212004	0.0
5	7.497841090381038E-5	0.0	0.0	1.7896409452603241	0.0
6	7.497841090381038E-5	0.0	0.0	2.3833574920021467	0.0
7	9.372301362976298E-5	0.0	0.0	2.82812942548355	0.0
8	1.1246761635571558E-4	0.0	0.0	3.287222235447581	0.0
9	1.1246761635571558E-4	0.0	0.0	3.6727612243149736	0.0
10	1.1246761635571558E-4	0.0	0.0	4.487682827825763	0.0
11	1.1246761635571558E-4	0.0	0.0	4.984264843241699	0.0
12	1.3121221908166816E-4	0.0	0.0	5.588909493372752	0.0
13	1.4995682180762076E-4	0.0	0.0	5.775455779701432	0.0
14	1.4995682180762076E-4	0.0	0.0	5.903162758073347	0.0
15	1.6870142453357336E-4	0.0	0.0	6.037149178358456	0.0
16	1.6870142453357336E-4	0.0	0.0	6.179008331788466	0.0
17	1.6870142453357336E-4	0.0	0.0	6.321486057108431	0.0
18	1.6870142453357336E-4	0.0	0.0	6.453785463148205	0.0
19	1.6870142453357336E-4	0.0	0.0	6.68509386078646	0.0
20	1.8744602725952596E-4	0.0	0.0	6.805284253465268	0.0
21	2.2493523271143115E-4	0.0	0.0	6.917152042533753	0.0
22	2.2493523271143115E-4	0.0	0.0	7.054718681939519	0.0
23	2.2493523271143115E-4	0.0	0.0	7.168948290951474	0.0
24	2.2493523271143115E-4	0.0	0.0	7.268482131426283	0.0
25	2.2493523271143115E-4	0.0	0.0	7.3538825414457225	0.0
26	2.4367983543738375E-4	0.0	0.0	7.441644771408632	0.0
27	2.624244381633363E-4	0.0	0.0	7.545658571934943	0.0
28	2.624244381633363E-4	0.0	0.0	7.640225092687374	0.0
29	2.624244381633363E-4	0.0	0.0	7.740227548230331	0.0
30	2.624244381633363E-4	0.0	0.0	7.863004696085321	0.0
31	2.624244381633363E-4	0.0	0.0	7.968161917377915	0.0
32	2.624244381633363E-4	0.0	0.0	8.079729792802786	0.0
33	2.624244381633363E-4	0.0	0.0	8.185880478039854	0.0
34	2.624244381633363E-4	0.0	0.0	8.298048180751955	0.0
35	2.624244381633363E-4	0.0	0.0	8.41070324313493	0.0
36	2.624244381633363E-4	0.0	0.0	8.527069736857644	0.0
37	2.811690408892889E-4	0.0	0.0	8.645160734031144	0.0
38	2.999136436152415E-4	0.0	0.0	8.777216460235481	0.0
39	2.999136436152415E-4	0.0	0.0	8.959676423169903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	202995	0.0	44.52782	1
CGTTATT	14910	0.0	44.38129	1
CCGATGA	6095	0.0	42.23134	18
CGATGAA	6300	0.0	41.964287	19
GTTATTT	16160	0.0	41.310337	2
ACCACCG	7330	0.0	41.070942	14
GATGAAT	6905	0.0	40.177406	20
GTTTTTT	226625	0.0	39.931606	2
CCACCGA	7315	0.0	39.832535	15
CGTAAGG	445	0.0	39.4382	2
CGACCAC	9150	0.0	39.41803	12
TAATACG	620	0.0	38.830643	4
TGAATGA	6915	0.0	38.752712	22
CACCGAT	6850	0.0	38.627735	16
ATCGAAT	780	0.0	38.365383	43
ATGAATG	7165	0.0	38.185623	21
GCGACCA	9405	0.0	38.08612	11
ACCGATG	6755	0.0	38.03849	17
GACCACC	9270	0.0	37.694176	13
CCAATCG	395	0.0	37.594936	40
>>END_MODULE