FastQCFastQC Report
Sat 14 Jan 2017
SRR2935209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4009061
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3634439.065539287129829No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT92460.23062757089503003No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC76420.19061820211765299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72350.18046619894284474No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGT71880.17929385459587668No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCT69970.17452964671777257No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG63690.15886513076254016No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG61380.15310318301467601No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTC58530.14599428644263582No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA58010.14469722461194778No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC56810.14170400500266772No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC46500.11598725985960304No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA45450.11336819270148296No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA40520.10107104880669066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1350300.044.5900881
CGTTATT100400.044.2828671
GTTATTT112250.040.0690422
GTTTTTT1531400.039.4359742
CGTTGTT4750.037.421051
CTATGCG1150.037.1739161
TGCGTAG1850.036.486491
TAGCCGT2100.036.4285744
CGTTTCT13050.036.034481
CGCGTAT250.00210773336.036
TACGGGA14700.035.9693874
CGACGGT1450.035.68965528
ACGTAGG2900.034.913792
CGTTTTC11150.034.9103161
TATGGGA27700.034.7653434
CGGTCTA1750.034.71428731
ACGGGAT21850.034.702525
CGAACGG2550.034.4117662
TCGACGG1650.034.0909082
CGTTCTG8800.033.4943161
Produced by FastQC (version 0.11.5)