##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4009061
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20469207128552	33.0	31.0	34.0	30.0	34.0
2	32.36626681409936	34.0	31.0	34.0	31.0	34.0
3	32.228318551401436	34.0	31.0	34.0	30.0	34.0
4	35.911390722166615	37.0	35.0	37.0	35.0	37.0
5	33.44810692578636	37.0	35.0	37.0	30.0	37.0
6	34.4891232635273	37.0	35.0	37.0	29.0	37.0
7	35.71619588726637	37.0	35.0	37.0	32.0	37.0
8	35.740637770290846	37.0	35.0	37.0	35.0	37.0
9	37.86559994971391	39.0	38.0	39.0	35.0	39.0
10	37.407109295667986	39.0	37.0	39.0	34.0	39.0
11	37.281839562930074	39.0	37.0	39.0	34.0	39.0
12	37.53845950460719	39.0	37.0	39.0	35.0	39.0
13	37.57557193567272	39.0	37.0	39.0	35.0	39.0
14	38.83999096047678	40.0	38.0	41.0	35.0	41.0
15	38.935520562046825	40.0	38.0	41.0	35.0	41.0
16	38.87130228250456	40.0	38.0	41.0	35.0	41.0
17	38.7684881821454	40.0	38.0	41.0	35.0	41.0
18	38.239419405192386	39.0	38.0	40.0	35.0	41.0
19	37.603976093154984	38.0	37.0	40.0	34.0	41.0
20	36.95734512395796	38.0	35.0	40.0	34.0	41.0
21	36.8081411083543	37.0	35.0	40.0	33.0	41.0
22	36.75822967023949	37.0	35.0	40.0	33.0	41.0
23	36.60466553140498	36.0	35.0	40.0	33.0	41.0
24	36.301247349441674	36.0	35.0	40.0	33.0	41.0
25	36.08990833514382	36.0	35.0	40.0	32.0	41.0
26	36.31235718289145	36.0	35.0	40.0	33.0	41.0
27	36.2338545110688	36.0	35.0	40.0	33.0	41.0
28	36.17628492058365	36.0	35.0	40.0	33.0	41.0
29	36.09726791385813	36.0	35.0	40.0	33.0	41.0
30	35.69943710010898	36.0	35.0	40.0	31.0	41.0
31	35.30584418645663	36.0	35.0	40.0	30.0	41.0
32	34.546194981817436	36.0	35.0	40.0	22.0	41.0
33	33.566248555459744	36.0	33.0	40.0	16.0	41.0
34	32.65937011185412	36.0	33.0	40.0	10.0	41.0
35	31.998181120217428	35.0	31.0	40.0	8.0	41.0
36	31.586004553185894	35.0	30.0	40.0	7.0	41.0
37	31.400203938029378	35.0	29.0	40.0	7.0	41.0
38	31.223367267297753	35.0	27.0	40.0	7.0	41.0
39	31.00800337036528	35.0	25.0	40.0	7.0	41.0
40	30.84697214634549	35.0	24.0	40.0	7.0	41.0
41	30.638562995175178	35.0	23.0	40.0	7.0	41.0
42	30.55221559362654	35.0	23.0	40.0	7.0	41.0
43	30.3942107640667	35.0	22.0	40.0	7.0	41.0
44	30.256707992220623	35.0	22.0	40.0	7.0	41.0
45	30.231633791553683	35.0	23.0	40.0	7.0	41.0
46	30.149145398386306	35.0	22.0	40.0	7.0	41.0
47	30.09887377617851	35.0	22.0	40.0	7.0	41.0
48	30.01462985971029	35.0	22.0	40.0	7.0	41.0
49	29.941621242480473	35.0	22.0	40.0	7.0	41.0
50	29.844295210274925	35.0	20.0	40.0	7.0	41.0
51	29.189140798805507	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	28.0
9	65.0
10	88.0
11	94.0
12	125.0
13	169.0
14	249.0
15	523.0
16	854.0
17	1698.0
18	3066.0
19	5796.0
20	9688.0
21	14963.0
22	23265.0
23	37593.0
24	64897.0
25	120370.0
26	186037.0
27	208920.0
28	186809.0
29	152028.0
30	125385.0
31	116461.0
32	118702.0
33	136292.0
34	191809.0
35	282082.0
36	348853.0
37	354242.0
38	577102.0
39	740556.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.686398635490953	13.320051752767045	14.39928202638972	45.594267585352284
2	56.577612563141344	15.964710938546459	17.29222877875892	10.165447719553281
3	19.865674281334208	16.30578831302392	54.038813577543465	9.7897238280984
4	17.53410586668549	17.64191165961306	53.69090168495815	11.1330807887433
5	15.510365145354484	25.385894602252247	48.88803138690082	10.215708865492443
6	17.17010541870029	22.312307046463	51.382281287313916	9.135306247522799
7	51.70854222472544	4.2619206841701835	41.347911643150354	2.6816254479540222
8	53.18207929487728	3.850402874887661	40.72457365951777	2.2429441707172826
9	48.55131912435356	6.1484222864156965	41.792080489670774	3.508178099559972
10	21.56400214414298	23.16405262978039	47.26166551219849	8.010279713878138
11	15.92051106231609	17.249525512333186	55.17730959942989	11.652653825920833
12	14.42178106045281	16.255227845123834	56.42550711999643	12.89748397442693
13	14.777400493532026	15.880202371577784	56.8517914793514	12.49060565553879
14	12.767229034429759	18.18221274258486	56.15564342872309	12.894914794262297
15	12.602028255494242	18.970701618159463	56.17290432846993	12.25436579787636
16	14.709454408401367	19.393069848525627	54.1243198843819	11.773155858691101
17	14.80536215338205	18.60645173520682	53.820233715575796	12.767952395835334
18	14.373390676769448	18.560879966655534	54.588268923820316	12.477460432754702
19	14.372816976344335	19.378677450904338	53.714548119871466	12.533957452879863
20	14.71955652458269	19.97415354867387	53.462494085273335	11.84379584147011
21	15.004086991941504	18.82882300867959	54.801909973432686	11.365180025946225
22	14.440563513501042	17.98725437203375	54.50892366067765	13.063258453787558
23	13.092991101906406	18.64551325110793	54.96012657327988	13.301369073705787
24	13.122848467508973	19.082822636023746	54.537908003894174	13.256420892573098
25	13.616405437582516	20.321491740834073	53.282102716820724	12.780000104762687
26	13.318330651491708	20.533586293648312	52.82753243215805	13.320550622701925
27	13.39814984107251	19.71154841495303	53.572395131927394	13.317906612047059
28	13.085582883373439	20.171456607918913	53.55910024816285	13.1838602605448
29	13.281264615330123	20.077718947154956	53.48851015237732	13.15250628513759
30	13.85858184747002	20.606745569598466	52.612594320715	12.922078262216512
31	14.274614429663204	21.203493785701937	51.6947983580195	12.82709342661536
32	14.646546909612997	21.734366226904505	50.2975883879043	13.321498475578197
33	15.545660193247246	22.45104277535313	48.27412204503748	13.729174986362144
34	16.01754126465025	23.585373233283306	46.30730238327628	14.08978311879016
35	16.559363900923433	24.842325921206985	44.21693758214205	14.381372595727528
36	17.629414967744317	25.156963189135812	42.858739240934476	14.3548826021854
37	18.005039085212225	25.412534256774844	42.2351767658312	14.347249892181734
38	18.09293996773808	25.558004729785853	41.57215367887892	14.776901623597146
39	18.831267471360498	24.713118608073064	40.74522687482181	15.710387045744628
40	19.316742748488984	24.829654624861032	40.94811727733751	14.90548534931247
41	18.517428395327485	25.498689094528622	40.348725050579176	15.635157459564722
42	18.189471300137363	25.780051737800946	40.40025831485228	15.630218647209409
43	18.05432743477837	25.59372381712326	40.44026768362966	15.911681064468713
44	17.99615920037136	25.327127723923383	40.04334181994238	16.63337125576288
45	17.306571289386717	25.03646115636554	40.209814717211835	17.447152837035905
46	17.336877637930677	25.211938655959585	40.28571777780383	17.165465928305906
47	17.018848054444668	25.58681446852517	40.973958739964296	16.42037873706586
48	16.924611523745835	24.816484458580202	41.272831717950915	16.98607229972305
49	17.04890496802119	24.56001542505839	41.31508600143525	17.075993605485174
50	16.37727637469223	24.331084011941947	41.91632404695264	17.37531556641318
51	16.92683149495605	24.053562667168197	40.85183537990567	18.167770457970082
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9316.0
1	10544.0
2	11772.0
3	274895.5
4	538019.0
5	337968.0
6	137917.0
7	130304.5
8	122692.0
9	121767.0
10	120842.0
11	117700.0
12	114558.0
13	109864.0
14	105170.0
15	98384.0
16	91598.0
17	85299.5
18	79001.0
19	73646.0
20	68291.0
21	63444.0
22	58597.0
23	57830.5
24	57064.0
25	56689.0
26	58624.5
27	60935.0
28	65423.0
29	69911.0
30	74162.0
31	78413.0
32	85336.5
33	92260.0
34	97512.0
35	102764.0
36	108728.5
37	114693.0
38	120738.0
39	126783.0
40	132135.0
41	137487.0
42	144953.5
43	152420.0
44	160136.5
45	167853.0
46	190228.0
47	212603.0
48	207644.5
49	202686.0
50	195783.0
51	188880.0
52	167449.0
53	146018.0
54	133322.5
55	120627.0
56	113809.5
57	106992.0
58	99203.0
59	91414.0
60	83706.0
61	75998.0
62	68159.5
63	60321.0
64	52554.5
65	44788.0
66	36694.5
67	28601.0
68	24603.0
69	20605.0
70	17281.5
71	13958.0
72	11632.0
73	9306.0
74	7261.5
75	4170.0
76	3123.0
77	2533.0
78	1943.0
79	1372.0
80	801.0
81	559.5
82	318.0
83	195.5
84	73.0
85	70.0
86	67.0
87	46.5
88	26.0
89	17.0
90	8.0
91	8.0
92	8.0
93	6.0
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4009061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.415585799070648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91548501916006	22.708453212362876
2	7.1340973403839865	4.054391101492057
3	2.6743107730769244	2.279763216772389
4	1.5138346903055262	1.7206599811194494
5	1.0753255603716034	1.527800286133651
6	0.7888049099347969	1.344861215818825
7	0.6302364289917362	1.25359761252002
8	0.5199712795365214	1.1820230805378058
9	0.43704335622009793	1.1176958687927392
>10	4.450868488773081	28.536078899334523
>50	0.7167092308654919	13.677995988706282
>100	0.13105205175427595	5.922781065482783
>500	0.007777417934379809	1.447367257381729
>1k	0.0034769633117878833	2.0051189711852966
>5k	9.149903452073378E-4	1.7718990908490397
>10k+	9.149903452073378E-5	9.449513151510603
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363443	9.065539287129829	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT	9246	0.23062757089503003	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC	7642	0.19061820211765299	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7235	0.18046619894284474	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGT	7188	0.17929385459587668	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCT	6997	0.17452964671777257	No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	6369	0.15886513076254016	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG	6138	0.15310318301467601	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTC	5853	0.14599428644263582	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	5801	0.14469722461194778	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	5681	0.14170400500266772	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	4650	0.11598725985960304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	4545	0.11336819270148296	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	4052	0.10107104880669066	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2471748372000325E-4	0.0	0.0	0.056671624602369484	0.0
2	1.2471748372000325E-4	0.0	0.0	0.2105480560161095	0.0
3	1.2471748372000325E-4	0.0	0.0	0.44696251815574767	0.0
4	1.2471748372000325E-4	0.0	0.0	0.6464855486110089	0.0
5	1.2471748372000325E-4	0.0	0.0	1.1945440590701912	0.0
6	1.2471748372000325E-4	0.0	0.0	1.8390341279416802	0.0
7	1.2471748372000325E-4	0.0	0.0	2.301835766529868	0.0
8	1.2471748372000325E-4	0.0	0.0	2.790603585228561	0.0
9	1.2471748372000325E-4	0.0	0.0	3.172563350869443	0.0
10	1.2471748372000325E-4	0.0	0.0	3.6964017259902007	0.0
11	1.2471748372000325E-4	0.0	0.0	4.277684974112392	0.0
12	1.2471748372000325E-4	0.0	0.0	4.691198263134435	0.0
13	1.496609804640039E-4	0.0	0.0	4.9093041986639765	0.0
14	1.496609804640039E-4	0.0	0.0	5.050135181280604	0.0
15	1.496609804640039E-4	0.0	0.0	5.188347096739112	0.0
16	1.496609804640039E-4	0.0	0.0	5.391636595202717	0.0
17	1.496609804640039E-4	0.0	0.0	5.637629360092052	0.0
18	1.496609804640039E-4	0.0	0.0	5.895794551392458	0.0
19	1.496609804640039E-4	0.0	0.0	6.069725554188375	0.0
20	1.496609804640039E-4	0.0	0.0	6.239166727570371	0.0
21	1.496609804640039E-4	0.0	0.0	6.417437898799744	0.0
22	1.496609804640039E-4	0.0	0.0	6.610949546539701	0.0
23	1.496609804640039E-4	0.0	0.0	6.793560886202529	0.0
24	1.7460447720800455E-4	0.0	0.0	6.9375347494089015	0.0
25	2.2449147069600588E-4	0.0	0.0	7.061853137181001	0.0
26	2.494349674400065E-4	0.0	0.0	7.18467491514846	0.0
27	2.494349674400065E-4	0.0	0.0	7.309093076907535	0.0
28	2.494349674400065E-4	0.0	0.0	7.430917115005235	0.0
29	2.743784641840072E-4	0.0	0.0	7.560523524087062	0.0
30	2.743784641840072E-4	0.0	0.0	7.707989476837594	0.0
31	3.242654576720085E-4	0.0	0.0	7.831484729217141	0.0
32	3.242654576720085E-4	0.0	0.0	7.960567324867344	0.0
33	3.242654576720085E-4	0.0	0.0	8.094139749931468	0.0
34	3.242654576720085E-4	0.0	0.0	8.232675930847646	0.0
35	3.242654576720085E-4	0.0	0.0	8.362157622445755	0.0
36	3.242654576720085E-4	0.0	0.0	8.477396577403038	0.0
37	3.242654576720085E-4	0.0	0.0	8.607277364949049	0.0
38	3.242654576720085E-4	0.0	0.0	8.759307977603733	0.0
39	3.242654576720085E-4	0.0	0.0	8.947980586975355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	135030	0.0	44.590088	1
CGTTATT	10040	0.0	44.282867	1
GTTATTT	11225	0.0	40.069042	2
GTTTTTT	153140	0.0	39.435974	2
CGTTGTT	475	0.0	37.42105	1
CTATGCG	115	0.0	37.173916	1
TGCGTAG	185	0.0	36.48649	1
TAGCCGT	210	0.0	36.42857	44
CGTTTCT	1305	0.0	36.03448	1
CGCGTAT	25	0.002107733	36.0	36
TACGGGA	1470	0.0	35.969387	4
CGACGGT	145	0.0	35.689655	28
ACGTAGG	290	0.0	34.91379	2
CGTTTTC	1115	0.0	34.910316	1
TATGGGA	2770	0.0	34.765343	4
CGGTCTA	175	0.0	34.714287	31
ACGGGAT	2185	0.0	34.70252	5
CGAACGG	255	0.0	34.411766	2
TCGACGG	165	0.0	34.090908	2
CGTTCTG	880	0.0	33.494316	1
>>END_MODULE