SRR2935208.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	971098
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36806	3.7901427044438356	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	22311	2.2975024147923278	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	21996	2.265064905910629	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	21308	2.1942172674642517	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	8321	0.8568651155702103	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	7936	0.8172192713814672	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	6686	0.6884989980413923	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	6126	0.6308323155850387	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	4965	0.5112769257067773	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	4554	0.46895369983256063	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4104	0.42261440143013373	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	3704	0.38142391396130976	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3505	0.36093164644556985	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	2675	0.2754613849477601	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	2434	0.2506441162477937	No Hit
GTGAAGGGAATGTAAAGTTTTTAAAAATTATTTTTACAAATTTCTTAATTT	1884	0.1940071959781608	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1793	0.18463636007900336	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	1695	0.1745446906491415	No Hit
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1533	0.1578625432242678	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTACAGC	1410	0.14519646832760444	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGT	1391	0.14323992017283527	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1368	0.14087146714337792	Illumina PCR Primer Index 11 (95% over 23bp)
GAATAATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	1335	0.13747325192719995	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.13438396536703814	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	1283	0.13211848855625283	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1270	0.13077979771351605	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGT	1117	0.11502443625669087	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1074	0.11059645885379231	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATG	1074	0.11059645885379231	No Hit
GAATATGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	1062	0.1093607442297276	Illumina PCR Primer Index 11 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCGA	35	1.2116652E-7	45.000004	33
ATCCGGC	150	0.0	45.000004	6
GGCGCCG	35	1.2116652E-7	45.000004	32
TCGAATA	35	1.2116652E-7	45.000004	44
TGATCGT	25	3.8903567E-5	45.0	28
TATTTCG	20	7.032745E-4	45.0	15
ATTTACG	25	3.8903567E-5	45.0	1
AATCCGG	20	7.032745E-4	45.0	2
GTCGACC	40	6.8139343E-9	45.0	10
GCGCGAT	40	6.8139343E-9	45.0	42
GCGCGAC	40	6.8139343E-9	45.0	9
ATTACCG	20	7.032745E-4	45.0	36
TCGATCA	25	3.8903567E-5	45.0	17
AGTATCG	20	7.032745E-4	45.0	1
CGCTCGA	365	0.0	45.0	41
CTACGAC	20	7.032745E-4	45.0	12
GCTTACC	25	3.8903567E-5	45.0	21
GGACGTA	20	7.032745E-4	45.0	8
ACGTCGA	40	6.8139343E-9	45.0	8
CTCGATC	25	3.8903567E-5	45.0	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content