##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	971098
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.550888787743357	31.0	31.0	34.0	30.0	34.0
2	31.67905710855135	31.0	31.0	34.0	30.0	34.0
3	31.665045134476642	31.0	31.0	34.0	30.0	34.0
4	35.52282467886866	37.0	35.0	37.0	33.0	37.0
5	32.99179897394496	35.0	35.0	37.0	28.0	37.0
6	33.9546513328212	36.0	35.0	37.0	28.0	37.0
7	35.260012892622576	37.0	35.0	37.0	32.0	37.0
8	34.99912161285473	37.0	35.0	37.0	32.0	37.0
9	37.01421380746331	39.0	37.0	39.0	33.0	39.0
10	36.62146662849681	39.0	35.0	39.0	32.0	39.0
11	36.473771957104226	39.0	35.0	39.0	32.0	39.0
12	36.838812354674815	39.0	37.0	39.0	32.0	39.0
13	36.74452732885867	39.0	37.0	39.0	32.0	39.0
14	37.75256359296384	40.0	37.0	41.0	32.0	41.0
15	37.78220220822203	40.0	37.0	41.0	32.0	41.0
16	37.920202698388835	40.0	37.0	41.0	33.0	41.0
17	37.609302047785086	39.0	36.0	41.0	32.0	41.0
18	37.509027925090976	39.0	37.0	40.0	33.0	41.0
19	37.21344086796595	38.0	36.0	40.0	32.0	41.0
20	36.36009239026339	38.0	35.0	40.0	30.0	41.0
21	36.63594199555555	38.0	35.0	40.0	31.0	41.0
22	36.66443242597555	38.0	35.0	40.0	31.0	41.0
23	36.0716446743789	38.0	35.0	40.0	30.0	41.0
24	35.9140478097988	38.0	35.0	40.0	30.0	41.0
25	35.89986386543892	37.0	35.0	40.0	30.0	41.0
26	36.0238915124941	38.0	35.0	40.0	30.0	41.0
27	35.765088590440925	38.0	35.0	40.0	30.0	41.0
28	35.895932233410015	38.0	35.0	40.0	30.0	41.0
29	35.91118610068191	38.0	35.0	40.0	30.0	41.0
30	35.56054177848168	38.0	35.0	40.0	29.0	41.0
31	35.33762915792227	38.0	35.0	40.0	27.0	41.0
32	34.5485512275795	38.0	34.0	40.0	22.0	41.0
33	34.1955549285448	38.0	34.0	40.0	19.0	41.0
34	33.66717880172753	38.0	33.0	40.0	15.0	41.0
35	33.30689075664866	38.0	33.0	40.0	12.0	41.0
36	33.07762450339719	38.0	33.0	40.0	10.0	41.0
37	32.87570667430064	38.0	33.0	40.0	10.0	41.0
38	32.95672321434088	38.0	33.0	40.0	10.0	41.0
39	32.74169033403426	38.0	32.0	40.0	10.0	41.0
40	32.82773108378351	37.0	32.0	40.0	10.0	41.0
41	32.438027881840966	37.0	31.0	40.0	10.0	41.0
42	32.54338799997529	37.0	31.0	40.0	9.0	41.0
43	32.47305730214664	37.0	31.0	40.0	10.0	41.0
44	32.44707022360256	37.0	31.0	40.0	10.0	41.0
45	32.542228487753036	37.0	31.0	40.0	10.0	41.0
46	32.22297749557717	37.0	31.0	40.0	9.0	41.0
47	31.885993998545977	37.0	31.0	40.0	8.0	41.0
48	31.957358577610087	37.0	30.0	40.0	8.0	41.0
49	32.00916797274837	37.0	31.0	40.0	8.0	41.0
50	31.81615655680477	37.0	30.0	40.0	8.0	41.0
51	30.904359807146136	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	12.0
11	16.0
12	13.0
13	32.0
14	61.0
15	82.0
16	163.0
17	351.0
18	838.0
19	1963.0
20	4149.0
21	8229.0
22	13108.0
23	16224.0
24	18891.0
25	24076.0
26	28591.0
27	28148.0
28	24575.0
29	22115.0
30	22806.0
31	26283.0
32	32071.0
33	40356.0
34	54418.0
35	77720.0
36	100858.0
37	120666.0
38	170878.0
39	133364.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.004409441683535	17.146879099740705	18.574438419191473	23.274273039384287
2	36.62977372005709	34.70442735954558	17.6566113821674	11.009187538229922
3	19.503283911613455	34.82624822623463	35.80400742252584	9.866460439626072
4	17.601828033833865	19.105486778883286	51.16795627217851	12.124728915104347
5	26.67495968481039	26.33359351991251	33.69907053665027	13.292376258626835
6	16.05811153972102	38.39591884650159	35.85940862817141	9.686560985605984
7	51.93821838784552	5.88498792088955	37.820281784124774	4.356511907140165
8	49.50190403028324	17.115883257920416	26.943109758232435	6.4391029535639035
9	46.15898704353217	7.237992458021744	27.061635385923978	19.541385112522114
10	31.571787811322853	21.17294032116223	34.47417253459486	12.781099332920057
11	22.470234723992842	20.03814239139613	40.236927683920676	17.254695200690353
12	15.088281512267557	17.221948763152636	44.09009183419181	23.599677890387994
13	20.351396048596538	19.511522009107214	48.56935139398907	11.567730548307175
14	14.234711635694852	30.10828979155554	39.38871257071892	16.268286002030692
15	10.365895100185563	22.59813118758354	47.691685082247105	19.34428862998379
16	10.896840483658703	25.043507452388948	37.869401440431346	26.190250623521006
17	11.290003686548628	29.342764581947446	41.39221788120251	17.975013850301412
18	10.668954111737436	24.462206697985167	42.561924749098445	22.306914441178954
19	10.679354709823313	26.087892262160977	39.7193692088749	23.513383819140806
20	15.065626744159704	27.165950295438773	44.39448953658642	13.373933423815105
21	12.865024951137785	33.1332162150473	40.42166702021835	13.580091813596567
22	10.154278970814481	23.587217767928674	44.263812715091575	21.99469054616527
23	14.271680098198122	27.739836762098165	40.0739163297628	17.91456680994091
24	14.409874183656028	27.119508020817673	37.44472751462777	21.02589028089853
25	10.43416833316514	35.520307940084315	35.13991378830973	18.90560993844082
26	12.527674858768115	24.446039431653656	43.683438746655845	19.342846962922383
27	18.72426881735932	25.737876094894645	38.582305802298016	16.95554928544802
28	10.652374940531233	27.868762987875577	41.910703142216335	19.56815892937685
29	17.727767949269797	22.009004240560685	41.3351690560582	18.928058754111326
30	13.684818627986054	30.48322620374051	40.05270322871636	15.77925193955708
31	14.770702853882925	26.673929922623667	36.07740928310016	22.477957940393246
32	20.25789364204231	30.88627512362295	32.26533264407917	16.590498590255567
33	16.399580680837566	23.954225011275895	32.668999421273654	26.97719488661289
34	18.62829498155696	28.01797552873139	31.876494442373478	21.477235047338166
35	21.271488562431394	24.483007894156923	32.53904343330951	21.706460110102174
36	18.321322873695546	26.4294643794962	33.5017681016746	21.747444645133655
37	13.906423450568326	28.22784106238505	39.8332609067262	18.03247458032042
38	15.533035800712183	26.40248461020412	31.670336052592013	26.394143536491683
39	17.372911899725878	32.611847619910655	29.302294928009324	20.71294555235414
40	19.315867193630304	24.790082978237006	31.39538954873761	24.498660279395075
41	18.455603862843915	28.341217879142988	28.071832091096883	25.131346166916213
42	20.934653351155085	22.325656112977267	35.333715031850545	21.405975504017103
43	19.994377498460505	22.481871036702785	31.784330726661985	25.73942073817473
44	17.30566842893302	22.992942009972218	35.38633587959197	24.315053681502793
45	17.913125142879505	21.47589635649543	33.399100811658556	27.211877688966513
46	23.947634533280883	26.323810779138668	30.915005488632456	18.813549198947996
47	13.890977017767517	24.844042516821165	40.41919559097022	20.845784874441097
48	16.93361535087087	26.724182317335632	32.69175716560018	23.650445166193318
49	18.61573188287897	20.44860559902296	39.85004603036975	21.085616487728323
50	18.746923585467172	19.889856636508366	34.42567073560032	26.93754904242414
51	16.896337959711584	20.196004934620397	31.68248724639532	31.2251698592727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5123.0
1	6353.0
2	7583.0
3	31093.5
4	54604.0
5	35037.0
6	15470.0
7	15453.0
8	15436.0
9	16518.0
10	17600.0
11	18019.5
12	18439.0
13	18682.5
14	18926.0
15	17624.0
16	16322.0
17	15844.0
18	15366.0
19	13396.0
20	11426.0
21	10812.5
22	10199.0
23	9805.0
24	9411.0
25	9216.5
26	9573.5
27	10125.0
28	10686.5
29	11248.0
30	12019.0
31	12790.0
32	14041.5
33	15293.0
34	16547.5
35	17802.0
36	18830.5
37	19859.0
38	21405.0
39	22951.0
40	26123.0
41	29295.0
42	35582.5
43	41870.0
44	50880.5
45	59891.0
46	111889.0
47	163887.0
48	135448.5
49	107010.0
50	98924.5
51	90839.0
52	70802.0
53	50765.0
54	42188.5
55	33612.0
56	28465.5
57	23319.0
58	19371.0
59	15423.0
60	12040.0
61	8657.0
62	6762.5
63	4868.0
64	3793.5
65	2719.0
66	2193.0
67	1667.0
68	1278.0
69	889.0
70	687.5
71	486.0
72	467.0
73	448.0
74	325.0
75	154.0
76	106.0
77	93.0
78	80.0
79	52.0
80	24.0
81	28.0
82	32.0
83	20.0
84	8.0
85	5.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	971098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.61499924792678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82859245184962	28.522924480476703
2	10.166784338411802	7.6484717048638755
3	4.156657361544145	4.690579905851171
4	2.3485626455587223	3.533647285856011
5	1.4822564393656776	2.78775374259873
6	1.0405941171549555	2.348516816050841
7	0.7879230343494673	2.074640704113558
8	0.6184504391809931	1.8610410243738433
9	0.5030320809150387	1.7029396210772003
>10	2.8911054550061337	18.173555932828865
>50	0.10670058059561412	2.759867782477138
>100	0.05660494372903686	3.849158568754817
>500	0.004245370779619059	1.0944061867043493
>1k	0.006226543810107953	4.954104270261371
>5k	0.0011320988745650822	3.094678350321861
>10k+	0.0011320988745650822	10.903713623389704
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36806	3.7901427044438356	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	22311	2.2975024147923278	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	21996	2.265064905910629	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	21308	2.1942172674642517	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	8321	0.8568651155702103	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	7936	0.8172192713814672	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	6686	0.6884989980413923	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	6126	0.6308323155850387	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	4965	0.5112769257067773	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	4554	0.46895369983256063	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4104	0.42261440143013373	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	3704	0.38142391396130976	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3505	0.36093164644556985	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	2675	0.2754613849477601	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	2434	0.2506441162477937	No Hit
GTGAAGGGAATGTAAAGTTTTTAAAAATTATTTTTACAAATTTCTTAATTT	1884	0.1940071959781608	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1793	0.18463636007900336	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	1695	0.1745446906491415	No Hit
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1533	0.1578625432242678	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTACAGC	1410	0.14519646832760444	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGT	1391	0.14323992017283527	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1368	0.14087146714337792	Illumina PCR Primer Index 11 (95% over 23bp)
GAATAATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	1335	0.13747325192719995	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.13438396536703814	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	1283	0.13211848855625283	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1270	0.13077979771351605	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGT	1117	0.11502443625669087	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1074	0.11059645885379231	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATG	1074	0.11059645885379231	No Hit
GAATATGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	1062	0.1093607442297276	Illumina PCR Primer Index 11 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0297621867205987E-4	0.0	0.0	0.2980131768369413	0.0
2	1.0297621867205987E-4	0.0	0.0	1.8427594331365114	0.0
3	1.0297621867205987E-4	0.0	0.0	2.4015083956511085	0.0
4	1.0297621867205987E-4	0.0	0.0	3.642989687961462	0.0
5	1.0297621867205987E-4	0.0	0.0	7.85317238836863	0.0
6	1.0297621867205987E-4	0.0	0.0	9.234701338072986	0.0
7	1.0297621867205987E-4	0.0	0.0	11.10207208747212	0.0
8	1.0297621867205987E-4	0.0	0.0	13.310397096894443	0.0
9	1.0297621867205987E-4	0.0	0.0	14.052958609738667	0.0
10	1.0297621867205987E-4	0.0	0.0	18.24038356581931	0.0
11	1.0297621867205987E-4	0.0	0.0	21.379407639599712	0.0
12	1.0297621867205987E-4	0.0	0.0	25.908713641671593	0.0
13	1.0297621867205987E-4	0.0	0.0	26.815625199516425	0.0
14	1.0297621867205987E-4	0.0	0.0	27.208685426187674	0.0
15	1.0297621867205987E-4	0.0	0.0	28.144533301479356	0.0
16	1.0297621867205987E-4	0.0	0.0	29.421232460575556	0.0
17	1.0297621867205987E-4	0.0	0.0	30.944559663391335	0.0
18	1.0297621867205987E-4	0.0	0.0	32.45244043340631	0.0
19	1.0297621867205987E-4	0.0	0.0	34.560466605842045	0.0
20	1.0297621867205987E-4	0.0	0.0	35.5785924798527	0.0
21	1.0297621867205987E-4	0.0	0.0	36.61144395313347	0.0
22	1.0297621867205987E-4	0.0	0.0	37.80112820745177	0.0
23	1.0297621867205987E-4	0.0	0.0	38.82687432164416	0.0
24	1.0297621867205987E-4	0.0	0.0	39.539160826198795	0.0
25	1.0297621867205987E-4	0.0	0.0	40.10058717039887	0.0
26	1.0297621867205987E-4	0.0	0.0	40.61248195341768	0.0
27	1.0297621867205987E-4	0.0	0.0	41.07463922281788	0.0
28	1.0297621867205987E-4	0.0	0.0	41.50827207964593	0.0
29	1.0297621867205987E-4	0.0	0.0	41.94118410294327	0.0
30	1.0297621867205987E-4	0.0	0.0	42.42887947457414	0.0
31	1.0297621867205987E-4	0.0	0.0	42.83615041942214	0.0
32	1.0297621867205987E-4	0.0	0.0	43.18668146778183	0.0
33	1.0297621867205987E-4	0.0	0.0	43.517750010812506	0.0
34	1.0297621867205987E-4	0.0	0.0	43.85201081662201	0.0
35	1.0297621867205987E-4	0.0	0.0	44.16989840366266	0.0
36	1.0297621867205987E-4	0.0	0.0	44.45421574341621	0.0
37	1.0297621867205987E-4	0.0	0.0	44.75665689765606	0.0
38	1.0297621867205987E-4	0.0	0.0	45.04952126355939	0.0
39	1.0297621867205987E-4	0.0	0.0	45.35494872814073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCGA	35	1.2116652E-7	45.000004	33
ATCCGGC	150	0.0	45.000004	6
GGCGCCG	35	1.2116652E-7	45.000004	32
TCGAATA	35	1.2116652E-7	45.000004	44
TGATCGT	25	3.8903567E-5	45.0	28
TATTTCG	20	7.032745E-4	45.0	15
ATTTACG	25	3.8903567E-5	45.0	1
AATCCGG	20	7.032745E-4	45.0	2
GTCGACC	40	6.8139343E-9	45.0	10
GCGCGAT	40	6.8139343E-9	45.0	42
GCGCGAC	40	6.8139343E-9	45.0	9
ATTACCG	20	7.032745E-4	45.0	36
TCGATCA	25	3.8903567E-5	45.0	17
AGTATCG	20	7.032745E-4	45.0	1
CGCTCGA	365	0.0	45.0	41
CTACGAC	20	7.032745E-4	45.0	12
GCTTACC	25	3.8903567E-5	45.0	21
GGACGTA	20	7.032745E-4	45.0	8
ACGTCGA	40	6.8139343E-9	45.0	8
CTCGATC	25	3.8903567E-5	45.0	16
>>END_MODULE