FastQCFastQC Report
Sat 14 Jan 2017
SRR2935207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783396
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279803.5716291632839585No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC90801.159056211673279TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG88871.1344198847070959No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC78871.006770522188012No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC43400.5539982333328227No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC41890.5347231795924412TruSeq Adapter, Index 23 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30920.3946918289090064No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC26110.3332924855373272TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT22650.2891258061057243No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT21530.2748290775035869No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT19860.2535116339629TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT19310.2464909190243504No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.2178974618200757No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.1950482259291597No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG15120.19300583612885436No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.18981460206587727No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTC14550.18572982246526662No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTA13910.17756026326404525No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13450.1716883925881674No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG11910.15203039076022853TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC11130.14207374048374002TruSeq Adapter, Index 23 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10650.135946571082824No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC10600.1353083242702286No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.13250003829480875No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTC9660.12330928419343475No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGT9420.12024569949297673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG207.0319086E-445.0000041
CTATCGA207.0319086E-445.00000441
AATTGCG207.0319086E-445.0000041
CTCGAAT351.211265E-745.00000443
CGGTCTA207.0319086E-445.00000431
CGATCTC207.0319086E-445.00000434
GCGAGAC502.1827873E-1145.021
TCGCCAA253.8896644E-545.042
CGTTGTT453.8380676E-1045.01
TATAGCG453.8380676E-1045.01
CGTTATT5900.044.9999961
CGAGCCC302.1646138E-644.99999634
CCGACTA302.1646138E-644.99999613
TGTAACG302.1646138E-644.9999961
AACACGT600.044.99999641
CGTTTTT88300.043.292751
CGATGAA9600.042.8906219
CCGATGA9450.042.85714318
GCTACGG603.6379788E-1241.2499962
GCGTAAG556.002665E-1140.9090921
Produced by FastQC (version 0.11.5)