FastQCFastQC Report
Sat 14 Jan 2017
SRR2935204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350278
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104682.9884834331588053No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC67651.9313231204928658No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG66211.890212916597674No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC63771.8205539599974878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32940.9403959141025129No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC30820.8798725583679249RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCGAACT23360.6668988631886673No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC22400.6394920605918728No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC18390.5250115622448456Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT16680.4761931951193053No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT13910.39711315012647097RNA PCR Primer, Index 33 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12960.36999183505672634No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT12860.36713695978622696TruSeq Adapter, Index 1 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTA12250.3497222206361804No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTC11540.32945260621563444No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.2980489782401407No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.20954784485465833No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG7130.2035526067866095No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT6870.19612993108331098No Hit
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC6720.19184761817756182RNA PCR Primer, Index 33 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.18128457967671394No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC5570.15901655256681835No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.15073741428237No Hit
ACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC5210.14873900159302042RNA PCR Primer, Index 33 (95% over 24bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG4550.12989682480772413No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTAGCGAAC4160.11876281125277637No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG4040.11533696092817705TruSeq Adapter, Index 1 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.11076916049537797No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGT3820.1090562353330783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG453.8380676E-1045.0000042
CGGGTAC253.8852333E-545.06
AGGTAAT207.026564E-445.035
CCCTCGA406.7921064E-945.041
ATTAGCG207.026564E-445.01
CTATCTC207.026564E-445.03
CGCATGG253.8852333E-545.02
CGACCCG253.8852333E-545.032
CGACAGG253.8852333E-545.02
CGTTCTG253.8852333E-545.01
ACCTCGC207.026564E-445.021
CGTTATT2100.045.01
CGTAGTT253.8852333E-545.016
ACTACGG207.026564E-445.02
TTGAACG207.026564E-445.01
TAGGGTG351.2087003E-745.05
CGGGATC253.8852333E-545.06
TACCTAT700.045.011
CGAATGC950.045.045
GCGATGT207.026564E-445.09
Produced by FastQC (version 0.11.5)