SRR2935203.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	342416
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11813	3.449897201065371	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	7230	2.111466753890005	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	7149	2.0878113172281667	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	6209	1.813291434979674	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4514	1.3182795196486146	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3918	1.144222232605953	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACACTAT	2113	0.6170856502032614	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	2085	0.6089084622213916	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	1820	0.5315172188215503	No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1774	0.5180832671370497	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	1590	0.4643474603990468	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1540	0.44974533900285035	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	1385	0.4044787626746414	Illumina PCR Primer Index 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	1373	0.40097425353955424	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACACTATCGTA	1269	0.3706018410354656	No Hit
TCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	771	0.22516471192934912	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.2242885846455773	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.2108546329610766	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	693	0.20238540255128265	No Hit
ACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	654	0.1909957478622494	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	650	0.18982757815055373	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	0.16734031120041118	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	506	0.14777346852950796	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	473	0.13813606840801831	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	472	0.13784402598009438	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.13696789869632262	No Hit
GAATATGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	402	0.11740105602541938	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCACACTATCGT	366	0.10688752862015793	No Hit
ACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	359	0.10484323162469043	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT	352	0.10279893462922293	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	348	0.10163076491752723	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATG	347	0.1013387224896033	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTAT	30	2.1610213E-6	45.000004	10
GCGAACC	30	2.1610213E-6	45.000004	33
AGATTGG	30	2.1610213E-6	45.000004	2
CGGGAAC	30	2.1610213E-6	45.000004	6
CGGCGCA	20	7.026342E-4	45.0	22
CGGCGAA	40	6.7902874E-9	45.0	31
ACCGGCG	20	7.026342E-4	45.0	20
CGAACCC	35	1.2085911E-7	45.0	34
TTGCTAG	20	7.026342E-4	45.0	1
GGCGTCA	20	7.026342E-4	45.0	36
CGTCTGG	20	7.026342E-4	45.0	2
AGGCGTC	20	7.026342E-4	45.0	35
TCGATTG	20	7.026342E-4	45.0	1
CACTGGT	20	7.026342E-4	45.0	3
TATGCGG	35	1.2085911E-7	45.0	2
CTTGCTC	20	7.026342E-4	45.0	34
AGGTTAG	20	7.026342E-4	45.0	1
CGGGACA	20	7.026342E-4	45.0	6
GCTAGCA	20	7.026342E-4	45.0	1
TTAGTGG	55	1.8189894E-12	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content