##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935202.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785762
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838167282205045	33.0	31.0	34.0	30.0	34.0
2	31.979288894092612	33.0	31.0	34.0	30.0	34.0
3	31.916081459780443	33.0	31.0	34.0	30.0	34.0
4	35.684616970533064	37.0	35.0	37.0	33.0	37.0
5	33.21568490204413	37.0	35.0	37.0	30.0	37.0
6	34.22747982213444	37.0	35.0	37.0	28.0	37.0
7	35.52590988110904	37.0	35.0	37.0	32.0	37.0
8	35.504403368959046	37.0	35.0	37.0	33.0	37.0
9	37.634827085046105	39.0	37.0	39.0	35.0	39.0
10	37.089444386468166	39.0	37.0	39.0	33.0	39.0
11	36.95055118470987	39.0	37.0	39.0	33.0	39.0
12	37.29635182154393	39.0	37.0	39.0	34.0	39.0
13	37.28920843716036	39.0	37.0	39.0	34.0	39.0
14	38.37895062372576	40.0	38.0	41.0	34.0	41.0
15	38.50072668314324	40.0	38.0	41.0	34.0	41.0
16	38.46256245529817	40.0	38.0	41.0	34.0	41.0
17	38.3870968563	40.0	38.0	41.0	34.0	41.0
18	38.10204616665097	39.0	38.0	40.0	34.0	41.0
19	37.69129329237097	39.0	37.0	40.0	34.0	41.0
20	37.291408848989896	39.0	35.0	40.0	34.0	41.0
21	37.18482059453117	38.0	35.0	40.0	33.0	41.0
22	37.12777660411168	38.0	35.0	40.0	33.0	41.0
23	36.89921884743727	38.0	35.0	40.0	33.0	41.0
24	36.571329486536634	38.0	35.0	40.0	32.0	41.0
25	36.3797956633179	38.0	35.0	40.0	32.0	41.0
26	36.60802253099539	38.0	35.0	40.0	33.0	41.0
27	36.536631957259324	38.0	35.0	40.0	32.0	41.0
28	36.520076053563294	38.0	35.0	40.0	32.0	41.0
29	36.49335040381184	38.0	35.0	40.0	32.0	41.0
30	36.11042529417305	38.0	35.0	40.0	31.0	41.0
31	35.721586180039246	38.0	35.0	40.0	30.0	41.0
32	35.15017778920335	38.0	35.0	40.0	24.0	41.0
33	34.587503595236214	38.0	35.0	40.0	20.0	41.0
34	34.08786120988289	38.0	34.0	40.0	15.0	41.0
35	33.666770599749036	38.0	33.0	40.0	12.0	41.0
36	33.40510612628251	38.0	33.0	40.0	10.0	41.0
37	33.33749277771132	38.0	33.0	40.0	10.0	41.0
38	33.261337402419564	38.0	33.0	40.0	10.0	41.0
39	33.147974832073835	38.0	33.0	40.0	10.0	41.0
40	33.02984364222245	38.0	33.0	40.0	10.0	41.0
41	32.881614280150984	38.0	33.0	40.0	10.0	41.0
42	32.873983470821955	38.0	33.0	40.0	10.0	41.0
43	32.77234582481719	38.0	33.0	40.0	10.0	41.0
44	32.64189792838035	38.0	32.0	40.0	9.0	41.0
45	32.59324451933283	38.0	32.0	40.0	8.0	41.0
46	32.45663954225325	38.0	31.0	40.0	8.0	41.0
47	32.35644762663504	37.0	31.0	40.0	8.0	41.0
48	32.2856348359936	37.0	31.0	40.0	8.0	41.0
49	32.24660520615657	37.0	31.0	40.0	8.0	41.0
50	32.155480921704026	37.0	31.0	40.0	8.0	41.0
51	31.365841056197677	36.0	28.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	15.0
11	21.0
12	17.0
13	38.0
14	68.0
15	78.0
16	166.0
17	323.0
18	533.0
19	1032.0
20	1798.0
21	2805.0
22	4685.0
23	7397.0
24	12219.0
25	20015.0
26	26526.0
27	27101.0
28	23062.0
29	19632.0
30	17849.0
31	18912.0
32	21271.0
33	26416.0
34	37994.0
35	57031.0
36	76584.0
37	94362.0
38	147809.0
39	139953.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.336951901466346	20.46815702464614	21.33254089660737	29.862350177280145
2	46.03811332184555	21.04339481929643	21.246000697412192	11.672491161445832
3	23.65678156999193	20.02832918873654	44.91563603228458	11.399253208986945
4	21.231619752545935	21.654139548616502	43.96865717609148	13.145583522746074
5	17.048164711452067	29.815134862719244	40.474469368587435	12.662231057241252
6	19.283956210659206	29.054217434795778	41.23449594151919	10.427330413025828
7	60.27104899447925	6.35701395587977	29.72146782359035	3.6504692260506366
8	60.366752273589206	5.895932865167825	29.521279980452096	4.216034880790875
9	55.20017511663837	8.993053876364598	31.123928110547467	4.682842896449562
10	22.20048818853546	32.03870892204	35.847877601614734	9.912925287809795
11	17.64974636085736	23.201045609230274	43.79061344274729	15.35859458716507
12	17.708033730315286	19.773162866109587	47.106248456911885	15.41255494666324
13	18.548873577495474	20.543370638946655	47.77197166572066	13.135784117837208
14	14.410215815984992	23.73428595427114	46.239446549973145	15.61605167977072
15	12.965376284422	24.57652571643831	47.109302816883485	15.348795182256206
16	14.208500792860942	24.85319982386524	45.412478587663955	15.525820795609865
17	14.138250513514269	24.599560681224087	43.838592347301095	17.423596457960553
18	13.500143809448662	24.369974623359237	47.23834952568335	14.891532041508752
19	13.834341696340621	25.677367956200474	45.85943326350727	14.62885708395163
20	14.033129624491893	27.060229433339867	45.213817924511496	13.692823017656746
21	14.364018621414626	25.4437094183735	46.2963340044441	13.895937955767776
22	13.078005808374545	24.42978917280296	44.118702609696065	18.373502409126427
23	12.475278773979907	24.533637411837173	44.62343559500205	18.36764821918087
24	13.24854090678857	25.069677586851995	44.09490405491739	17.58687745144204
25	12.182823806699739	26.383688699631698	42.818563381787364	18.614924111881205
26	12.194404921592033	27.94293946513066	43.65291271402791	16.20974289924939
27	13.933735660416257	25.761744650415775	44.47860802634895	15.825911662819022
28	11.297186679936164	25.799287825066624	44.7059033142351	18.197622180762114
29	13.184399347385087	24.940249083055683	44.4457736566543	17.429577912904925
30	16.31015498331556	26.12063194707812	41.4274296797249	16.141783389881414
31	14.667927438588274	28.334661131487653	39.986789893122854	17.01062153680122
32	13.79374416171818	28.453526640382204	39.43344167826899	18.319287519630624
33	16.140765269890885	25.884682639272448	38.39139586796002	19.583156222876646
34	15.146189304140439	26.8490968003034	37.79299584352514	20.21171805203102
35	15.829093287789433	27.911123215426553	35.577439479129815	20.6823440176542
36	17.88289583868907	27.732952217083547	36.11322512414701	18.27092682008038
37	17.22009972485307	26.875949715053665	39.144550130955686	16.759400429137575
38	15.181314443813775	26.470865223820955	38.09537748071299	20.25244285165228
39	17.84611625403112	24.32746811375455	36.75845866814633	21.067956964068
40	19.514051328519322	25.080367846752576	34.80875888627854	20.596821938449555
41	17.994761772648715	26.367271514784374	33.625957987278596	22.012008725288318
42	17.317202918950013	26.111341602164522	37.35685869258121	19.21459678630425
43	17.897276783555327	24.673883440532883	38.68054194527096	18.74829783064083
44	17.074763096204705	24.899524283434424	35.90451052608805	22.12120209427282
45	19.406766934517066	23.22013535905274	35.09370521862854	22.27939248780165
46	19.460854559014052	24.706336015231077	35.54969570938783	20.283113716367044
47	16.600446445615848	25.739600540621716	37.99483813164801	19.66511488211443
48	16.204652299296736	23.89934865773606	38.94639852779849	20.949600515168715
49	18.33914085944599	22.00882710031791	38.80755241408976	20.844479626146338
50	17.020548206708902	21.577526019329007	37.15807076442994	24.243855009532147
51	16.984659477042666	21.185168027977937	34.83064846607497	26.999524028904425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4958.0
1	6008.5
2	7059.0
3	31862.0
4	56665.0
5	36740.5
6	16816.0
7	16366.5
8	15917.0
9	16539.5
10	17162.0
11	17553.0
12	17944.0
13	17475.5
14	17007.0
15	16156.0
16	15305.0
17	14279.0
18	13253.0
19	12569.5
20	11886.0
21	11391.5
22	10897.0
23	10874.0
24	10851.0
25	11098.0
26	11519.5
27	11694.0
28	11762.0
29	11830.0
30	12735.0
31	13640.0
32	14177.0
33	14714.0
34	16105.0
35	17496.0
36	18678.5
37	19861.0
38	20651.0
39	21441.0
40	24103.0
41	26765.0
42	32298.0
43	37831.0
44	41921.5
45	46012.0
46	55221.0
47	64430.0
48	72550.0
49	80670.0
50	77031.0
51	73392.0
52	58363.0
53	43334.0
54	33733.0
55	24132.0
56	19618.0
57	15104.0
58	13122.5
59	11141.0
60	10110.0
61	9079.0
62	7780.5
63	6482.0
64	5312.5
65	4143.0
66	3179.0
67	2215.0
68	1817.0
69	1419.0
70	1057.0
71	695.0
72	597.5
73	500.0
74	395.0
75	233.0
76	176.0
77	146.5
78	117.0
79	80.5
80	44.0
81	37.5
82	31.0
83	19.0
84	7.0
85	7.5
86	8.0
87	5.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	785762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.927122814978055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05663116611801	34.17005609293671
2	10.07217800564668	9.050279565480182
3	4.139812727790545	5.579696245573654
4	2.3512923601358313	4.225472025509685
5	1.5546809060816291	3.492367000281535
6	1.05484929718208	2.843480635547556
7	0.8375621380850857	2.634047993004698
8	0.6823094350995231	2.452335983082766
9	0.5004695871548486	2.023619274654052
>10	2.623996922908012	18.878420501474537
>50	0.08201230590155408	2.497280062097439
>100	0.03722538770209824	2.990382457577819
>500	0.0031990567556342314	1.1102057824078997
>1k	0.0031990567556342314	2.4606926562419646
>5k	2.9082334142129375E-4	0.7093453210182991
>10k+	2.9082334142129375E-4	4.882318403111214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37367	4.755511210773745	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	5429	0.6909216785744284	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3684	0.46884425563974846	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	2608	0.3319071169132638	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	1878	0.2390036677772659	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	1871	0.23811281278555085	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	1623	0.20655109307907482	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1306	0.16620808845426477	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	1292	0.1644263784708347	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1214	0.1544997085631527	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	1185	0.15080902359747608	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC	1156	0.14711833863179946	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1016	0.1293012387974985	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	954	0.12141080887087949	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	950	0.12090174887561372	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	950	0.12090174887561372	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	917	0.11670200391467137	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	913	0.11619294391940563	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21978665295598412	0.0
2	0.0	0.0	0.0	1.04968171023796	0.0
3	0.0	0.0	0.0	1.5135626309238674	0.0
4	0.0	0.0	0.0	2.1021632504498817	0.0
5	0.0	0.0	0.0	3.6722315408482467	0.0
6	0.0	0.0	0.0	4.721276926092124	0.0
7	0.0	0.0	0.0	5.772358551317065	0.0
8	0.0	0.0	0.0	7.3499354766456	0.0
9	0.0	0.0	0.0	8.061728615025924	0.0
10	0.0	0.0	0.0	9.97808496720381	0.0
11	0.0	0.0	0.0	13.479781409638033	0.0
12	0.0	0.0	0.0	15.774114808300732	0.0
13	0.0	0.0	0.0	16.69105912477315	0.0
14	0.0	0.0	0.0	17.071581471234293	0.0
15	0.0	0.0	0.0	17.71719681023007	0.0
16	0.0	0.0	0.0	19.067605712671266	0.0
17	0.0	0.0	0.0	20.83404389624339	0.0
18	0.0	0.0	0.0	22.532268040449907	0.0
19	0.0	0.0	0.0	23.663399349930387	0.0
20	0.0	0.0	0.0	24.814765794222676	0.0
21	1.2726499881643552E-4	0.0	0.0	26.172047006599964	0.0
22	1.2726499881643552E-4	0.0	0.0	27.577561653528676	0.0
23	1.2726499881643552E-4	0.0	0.0	28.84957531669895	0.0
24	1.2726499881643552E-4	0.0	0.0	29.71574089864361	0.0
25	1.2726499881643552E-4	0.0	0.0	30.355120252697382	0.0
26	1.2726499881643552E-4	0.0	0.0	30.926540097383178	0.0
27	1.2726499881643552E-4	0.0	0.0	31.448199327531746	0.0
28	1.2726499881643552E-4	0.0	0.0	31.97507642263179	0.0
29	1.2726499881643552E-4	0.0	0.0	32.474591542986296	0.0
30	1.2726499881643552E-4	0.0	0.0	32.99230555817156	0.0
31	1.2726499881643552E-4	0.0	0.0	33.4471506639415	0.0
32	1.2726499881643552E-4	0.0	0.0	33.87005225500852	0.0
33	1.2726499881643552E-4	0.0	0.0	34.24268417154304	0.0
34	1.2726499881643552E-4	0.0	0.0	34.60997095812727	0.0
35	1.2726499881643552E-4	0.0	0.0	34.97496697473281	0.0
36	1.2726499881643552E-4	0.0	0.0	35.30203802169105	0.0
37	1.2726499881643552E-4	0.0	0.0	35.64285368852146	0.0
38	2.5452999763287104E-4	0.0	0.0	35.97234277045721	0.0
39	2.5452999763287104E-4	0.0	0.0	36.28757817252552	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	25	3.889676E-5	45.0	6
CAACGTA	20	7.0319226E-4	45.0	33
ACGTTAG	20	7.0319226E-4	45.0	1
CGACCTC	20	7.0319226E-4	45.0	12
GCTTGCG	25	3.889676E-5	45.0	1
TGTTACG	20	7.0319226E-4	45.0	1
TATAGCG	25	3.889676E-5	45.0	1
GTCGTAG	30	2.1646229E-6	44.999996	1
CGTTATT	1120	0.0	44.598213	1
CGTTTTT	15730	0.0	44.2562	1
TCGTAGG	75	0.0	42.0	2
TAGCACG	60	3.6379788E-12	41.249996	1
ACCTGGT	50	1.0804797E-9	40.5	11
GGATTAC	50	1.0804797E-9	40.5	8
ATAGGGT	90	0.0	40.0	4
ACAACGG	40	3.4563527E-7	39.375	2
TTTGGCG	40	3.4563527E-7	39.375	1
TACGGGA	325	0.0	38.76923	4
GTGTACG	35	6.2457675E-6	38.571426	1
ATTTGCG	35	6.2457675E-6	38.571426	1
>>END_MODULE