##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935202.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 785762 Sequences flagged as poor quality 0 Sequence length 51 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838167282205045 33.0 31.0 34.0 30.0 34.0 2 31.979288894092612 33.0 31.0 34.0 30.0 34.0 3 31.916081459780443 33.0 31.0 34.0 30.0 34.0 4 35.684616970533064 37.0 35.0 37.0 33.0 37.0 5 33.21568490204413 37.0 35.0 37.0 30.0 37.0 6 34.22747982213444 37.0 35.0 37.0 28.0 37.0 7 35.52590988110904 37.0 35.0 37.0 32.0 37.0 8 35.504403368959046 37.0 35.0 37.0 33.0 37.0 9 37.634827085046105 39.0 37.0 39.0 35.0 39.0 10 37.089444386468166 39.0 37.0 39.0 33.0 39.0 11 36.95055118470987 39.0 37.0 39.0 33.0 39.0 12 37.29635182154393 39.0 37.0 39.0 34.0 39.0 13 37.28920843716036 39.0 37.0 39.0 34.0 39.0 14 38.37895062372576 40.0 38.0 41.0 34.0 41.0 15 38.50072668314324 40.0 38.0 41.0 34.0 41.0 16 38.46256245529817 40.0 38.0 41.0 34.0 41.0 17 38.3870968563 40.0 38.0 41.0 34.0 41.0 18 38.10204616665097 39.0 38.0 40.0 34.0 41.0 19 37.69129329237097 39.0 37.0 40.0 34.0 41.0 20 37.291408848989896 39.0 35.0 40.0 34.0 41.0 21 37.18482059453117 38.0 35.0 40.0 33.0 41.0 22 37.12777660411168 38.0 35.0 40.0 33.0 41.0 23 36.89921884743727 38.0 35.0 40.0 33.0 41.0 24 36.571329486536634 38.0 35.0 40.0 32.0 41.0 25 36.3797956633179 38.0 35.0 40.0 32.0 41.0 26 36.60802253099539 38.0 35.0 40.0 33.0 41.0 27 36.536631957259324 38.0 35.0 40.0 32.0 41.0 28 36.520076053563294 38.0 35.0 40.0 32.0 41.0 29 36.49335040381184 38.0 35.0 40.0 32.0 41.0 30 36.11042529417305 38.0 35.0 40.0 31.0 41.0 31 35.721586180039246 38.0 35.0 40.0 30.0 41.0 32 35.15017778920335 38.0 35.0 40.0 24.0 41.0 33 34.587503595236214 38.0 35.0 40.0 20.0 41.0 34 34.08786120988289 38.0 34.0 40.0 15.0 41.0 35 33.666770599749036 38.0 33.0 40.0 12.0 41.0 36 33.40510612628251 38.0 33.0 40.0 10.0 41.0 37 33.33749277771132 38.0 33.0 40.0 10.0 41.0 38 33.261337402419564 38.0 33.0 40.0 10.0 41.0 39 33.147974832073835 38.0 33.0 40.0 10.0 41.0 40 33.02984364222245 38.0 33.0 40.0 10.0 41.0 41 32.881614280150984 38.0 33.0 40.0 10.0 41.0 42 32.873983470821955 38.0 33.0 40.0 10.0 41.0 43 32.77234582481719 38.0 33.0 40.0 10.0 41.0 44 32.64189792838035 38.0 32.0 40.0 9.0 41.0 45 32.59324451933283 38.0 32.0 40.0 8.0 41.0 46 32.45663954225325 38.0 31.0 40.0 8.0 41.0 47 32.35644762663504 37.0 31.0 40.0 8.0 41.0 48 32.2856348359936 37.0 31.0 40.0 8.0 41.0 49 32.24660520615657 37.0 31.0 40.0 8.0 41.0 50 32.155480921704026 37.0 31.0 40.0 8.0 41.0 51 31.365841056197677 36.0 28.0 40.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 6.0 10 15.0 11 21.0 12 17.0 13 38.0 14 68.0 15 78.0 16 166.0 17 323.0 18 533.0 19 1032.0 20 1798.0 21 2805.0 22 4685.0 23 7397.0 24 12219.0 25 20015.0 26 26526.0 27 27101.0 28 23062.0 29 19632.0 30 17849.0 31 18912.0 32 21271.0 33 26416.0 34 37994.0 35 57031.0 36 76584.0 37 94362.0 38 147809.0 39 139953.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.336951901466346 20.46815702464614 21.33254089660737 29.862350177280145 2 46.03811332184555 21.04339481929643 21.246000697412192 11.672491161445832 3 23.65678156999193 20.02832918873654 44.91563603228458 11.399253208986945 4 21.231619752545935 21.654139548616502 43.96865717609148 13.145583522746074 5 17.048164711452067 29.815134862719244 40.474469368587435 12.662231057241252 6 19.283956210659206 29.054217434795778 41.23449594151919 10.427330413025828 7 60.27104899447925 6.35701395587977 29.72146782359035 3.6504692260506366 8 60.366752273589206 5.895932865167825 29.521279980452096 4.216034880790875 9 55.20017511663837 8.993053876364598 31.123928110547467 4.682842896449562 10 22.20048818853546 32.03870892204 35.847877601614734 9.912925287809795 11 17.64974636085736 23.201045609230274 43.79061344274729 15.35859458716507 12 17.708033730315286 19.773162866109587 47.106248456911885 15.41255494666324 13 18.548873577495474 20.543370638946655 47.77197166572066 13.135784117837208 14 14.410215815984992 23.73428595427114 46.239446549973145 15.61605167977072 15 12.965376284422 24.57652571643831 47.109302816883485 15.348795182256206 16 14.208500792860942 24.85319982386524 45.412478587663955 15.525820795609865 17 14.138250513514269 24.599560681224087 43.838592347301095 17.423596457960553 18 13.500143809448662 24.369974623359237 47.23834952568335 14.891532041508752 19 13.834341696340621 25.677367956200474 45.85943326350727 14.62885708395163 20 14.033129624491893 27.060229433339867 45.213817924511496 13.692823017656746 21 14.364018621414626 25.4437094183735 46.2963340044441 13.895937955767776 22 13.078005808374545 24.42978917280296 44.118702609696065 18.373502409126427 23 12.475278773979907 24.533637411837173 44.62343559500205 18.36764821918087 24 13.24854090678857 25.069677586851995 44.09490405491739 17.58687745144204 25 12.182823806699739 26.383688699631698 42.818563381787364 18.614924111881205 26 12.194404921592033 27.94293946513066 43.65291271402791 16.20974289924939 27 13.933735660416257 25.761744650415775 44.47860802634895 15.825911662819022 28 11.297186679936164 25.799287825066624 44.7059033142351 18.197622180762114 29 13.184399347385087 24.940249083055683 44.4457736566543 17.429577912904925 30 16.31015498331556 26.12063194707812 41.4274296797249 16.141783389881414 31 14.667927438588274 28.334661131487653 39.986789893122854 17.01062153680122 32 13.79374416171818 28.453526640382204 39.43344167826899 18.319287519630624 33 16.140765269890885 25.884682639272448 38.39139586796002 19.583156222876646 34 15.146189304140439 26.8490968003034 37.79299584352514 20.21171805203102 35 15.829093287789433 27.911123215426553 35.577439479129815 20.6823440176542 36 17.88289583868907 27.732952217083547 36.11322512414701 18.27092682008038 37 17.22009972485307 26.875949715053665 39.144550130955686 16.759400429137575 38 15.181314443813775 26.470865223820955 38.09537748071299 20.25244285165228 39 17.84611625403112 24.32746811375455 36.75845866814633 21.067956964068 40 19.514051328519322 25.080367846752576 34.80875888627854 20.596821938449555 41 17.994761772648715 26.367271514784374 33.625957987278596 22.012008725288318 42 17.317202918950013 26.111341602164522 37.35685869258121 19.21459678630425 43 17.897276783555327 24.673883440532883 38.68054194527096 18.74829783064083 44 17.074763096204705 24.899524283434424 35.90451052608805 22.12120209427282 45 19.406766934517066 23.22013535905274 35.09370521862854 22.27939248780165 46 19.460854559014052 24.706336015231077 35.54969570938783 20.283113716367044 47 16.600446445615848 25.739600540621716 37.99483813164801 19.66511488211443 48 16.204652299296736 23.89934865773606 38.94639852779849 20.949600515168715 49 18.33914085944599 22.00882710031791 38.80755241408976 20.844479626146338 50 17.020548206708902 21.577526019329007 37.15807076442994 24.243855009532147 51 16.984659477042666 21.185168027977937 34.83064846607497 26.999524028904425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4958.0 1 6008.5 2 7059.0 3 31862.0 4 56665.0 5 36740.5 6 16816.0 7 16366.5 8 15917.0 9 16539.5 10 17162.0 11 17553.0 12 17944.0 13 17475.5 14 17007.0 15 16156.0 16 15305.0 17 14279.0 18 13253.0 19 12569.5 20 11886.0 21 11391.5 22 10897.0 23 10874.0 24 10851.0 25 11098.0 26 11519.5 27 11694.0 28 11762.0 29 11830.0 30 12735.0 31 13640.0 32 14177.0 33 14714.0 34 16105.0 35 17496.0 36 18678.5 37 19861.0 38 20651.0 39 21441.0 40 24103.0 41 26765.0 42 32298.0 43 37831.0 44 41921.5 45 46012.0 46 55221.0 47 64430.0 48 72550.0 49 80670.0 50 77031.0 51 73392.0 52 58363.0 53 43334.0 54 33733.0 55 24132.0 56 19618.0 57 15104.0 58 13122.5 59 11141.0 60 10110.0 61 9079.0 62 7780.5 63 6482.0 64 5312.5 65 4143.0 66 3179.0 67 2215.0 68 1817.0 69 1419.0 70 1057.0 71 695.0 72 597.5 73 500.0 74 395.0 75 233.0 76 176.0 77 146.5 78 117.0 79 80.5 80 44.0 81 37.5 82 31.0 83 19.0 84 7.0 85 7.5 86 8.0 87 5.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 785762.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.927122814978055 #Duplication Level Percentage of deduplicated Percentage of total 1 76.05663116611801 34.17005609293671 2 10.07217800564668 9.050279565480182 3 4.139812727790545 5.579696245573654 4 2.3512923601358313 4.225472025509685 5 1.5546809060816291 3.492367000281535 6 1.05484929718208 2.843480635547556 7 0.8375621380850857 2.634047993004698 8 0.6823094350995231 2.452335983082766 9 0.5004695871548486 2.023619274654052 >10 2.623996922908012 18.878420501474537 >50 0.08201230590155408 2.497280062097439 >100 0.03722538770209824 2.990382457577819 >500 0.0031990567556342314 1.1102057824078997 >1k 0.0031990567556342314 2.4606926562419646 >5k 2.9082334142129375E-4 0.7093453210182991 >10k+ 2.9082334142129375E-4 4.882318403111214 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37367 4.755511210773745 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC 5429 0.6909216785744284 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3684 0.46884425563974846 No Hit CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC 2608 0.3319071169132638 No Hit GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC 1878 0.2390036677772659 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG 1871 0.23811281278555085 No Hit CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT 1623 0.20655109307907482 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 1306 0.16620808845426477 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT 1292 0.1644263784708347 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.1544997085631527 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC 1185 0.15080902359747608 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC 1156 0.14711833863179946 No Hit CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG 1016 0.1293012387974985 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 0.12141080887087949 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT 950 0.12090174887561372 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 0.12090174887561372 No Hit TCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC 917 0.11670200391467137 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 913 0.11619294391940563 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.21978665295598412 0.0 2 0.0 0.0 0.0 1.04968171023796 0.0 3 0.0 0.0 0.0 1.5135626309238674 0.0 4 0.0 0.0 0.0 2.1021632504498817 0.0 5 0.0 0.0 0.0 3.6722315408482467 0.0 6 0.0 0.0 0.0 4.721276926092124 0.0 7 0.0 0.0 0.0 5.772358551317065 0.0 8 0.0 0.0 0.0 7.3499354766456 0.0 9 0.0 0.0 0.0 8.061728615025924 0.0 10 0.0 0.0 0.0 9.97808496720381 0.0 11 0.0 0.0 0.0 13.479781409638033 0.0 12 0.0 0.0 0.0 15.774114808300732 0.0 13 0.0 0.0 0.0 16.69105912477315 0.0 14 0.0 0.0 0.0 17.071581471234293 0.0 15 0.0 0.0 0.0 17.71719681023007 0.0 16 0.0 0.0 0.0 19.067605712671266 0.0 17 0.0 0.0 0.0 20.83404389624339 0.0 18 0.0 0.0 0.0 22.532268040449907 0.0 19 0.0 0.0 0.0 23.663399349930387 0.0 20 0.0 0.0 0.0 24.814765794222676 0.0 21 1.2726499881643552E-4 0.0 0.0 26.172047006599964 0.0 22 1.2726499881643552E-4 0.0 0.0 27.577561653528676 0.0 23 1.2726499881643552E-4 0.0 0.0 28.84957531669895 0.0 24 1.2726499881643552E-4 0.0 0.0 29.71574089864361 0.0 25 1.2726499881643552E-4 0.0 0.0 30.355120252697382 0.0 26 1.2726499881643552E-4 0.0 0.0 30.926540097383178 0.0 27 1.2726499881643552E-4 0.0 0.0 31.448199327531746 0.0 28 1.2726499881643552E-4 0.0 0.0 31.97507642263179 0.0 29 1.2726499881643552E-4 0.0 0.0 32.474591542986296 0.0 30 1.2726499881643552E-4 0.0 0.0 32.99230555817156 0.0 31 1.2726499881643552E-4 0.0 0.0 33.4471506639415 0.0 32 1.2726499881643552E-4 0.0 0.0 33.87005225500852 0.0 33 1.2726499881643552E-4 0.0 0.0 34.24268417154304 0.0 34 1.2726499881643552E-4 0.0 0.0 34.60997095812727 0.0 35 1.2726499881643552E-4 0.0 0.0 34.97496697473281 0.0 36 1.2726499881643552E-4 0.0 0.0 35.30203802169105 0.0 37 1.2726499881643552E-4 0.0 0.0 35.64285368852146 0.0 38 2.5452999763287104E-4 0.0 0.0 35.97234277045721 0.0 39 2.5452999763287104E-4 0.0 0.0 36.28757817252552 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTCA 25 3.889676E-5 45.0 6 CAACGTA 20 7.0319226E-4 45.0 33 ACGTTAG 20 7.0319226E-4 45.0 1 CGACCTC 20 7.0319226E-4 45.0 12 GCTTGCG 25 3.889676E-5 45.0 1 TGTTACG 20 7.0319226E-4 45.0 1 TATAGCG 25 3.889676E-5 45.0 1 GTCGTAG 30 2.1646229E-6 44.999996 1 CGTTATT 1120 0.0 44.598213 1 CGTTTTT 15730 0.0 44.2562 1 TCGTAGG 75 0.0 42.0 2 TAGCACG 60 3.6379788E-12 41.249996 1 ACCTGGT 50 1.0804797E-9 40.5 11 GGATTAC 50 1.0804797E-9 40.5 8 ATAGGGT 90 0.0 40.0 4 ACAACGG 40 3.4563527E-7 39.375 2 TTTGGCG 40 3.4563527E-7 39.375 1 TACGGGA 325 0.0 38.76923 4 GTGTACG 35 6.2457675E-6 38.571426 1 ATTTGCG 35 6.2457675E-6 38.571426 1 >>END_MODULE