FastQCFastQC Report
Sat 14 Jan 2017
SRR2935201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563169
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178523.1699187987975193No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC50920.9041690860114814No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG50850.9029261198681036No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC43540.7731249411810664RNA PCR Primer, Index 30 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC36410.6465199611484297No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC33360.5923621506155345Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC28030.4977191571269015RNA PCR Primer, Index 30 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27930.49594349120779024No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA20740.3682731116236867No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.3432362221642171No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT18550.3293860279951489RNA PCR Primer, Index 30 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT17010.30204077284083464No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14130.25090159437042875No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.2354533008741603No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.21521070939629133No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG12080.21450044302864682No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.21023884482277966No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT11480.20384644751397893No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.18058522397362073No Hit
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC9920.17614605917584242Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT8890.15785670020899586No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC8870.15750156702517362No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA8600.15270726904357307No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8440.14986620357299496No Hit
GATAGAGGTTTTTTAGGATTAAGGATAAGCTAATTTTATTAAATGAAAATA8410.14933350379726157No Hit
ACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC6480.11506315155841318Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG5850.10387645626801191RNA PCR Primer, Index 30 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC5660.10050269102170042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCG351.2104465E-745.07
TTCTACG207.030219E-445.01
CGGCGAA253.888263E-545.031
TCCGCTA351.2104465E-745.02
GATCGAC253.888263E-545.010
CGACGGT502.1827873E-1145.028
CTATACG207.030219E-445.01
GACCGTC406.8048394E-945.09
CGGTTAG207.030219E-445.01
GGCGTAT406.8048394E-945.07
CAGCACG207.030219E-445.01
ATCCGCT351.2104465E-745.01
AACGGGC253.888263E-545.04
CACTCGA207.030219E-445.041
TGACCGG253.888263E-545.02
CGCTAGT351.2104465E-745.04
GCGATCG351.2104465E-745.033
TCGTAAG207.030219E-445.01
GGTTGCG207.030219E-445.01
ACCGTCT253.888263E-545.010
Produced by FastQC (version 0.11.5)