##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563169
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734935694258738	31.0	31.0	34.0	30.0	34.0
2	31.838345150390026	33.0	31.0	34.0	30.0	34.0
3	31.82566512006165	33.0	31.0	34.0	30.0	34.0
4	35.63344750865193	37.0	35.0	37.0	33.0	37.0
5	33.120731077172216	37.0	35.0	37.0	28.0	37.0
6	34.11958399698847	37.0	35.0	37.0	28.0	37.0
7	35.41458425446003	37.0	35.0	37.0	32.0	37.0
8	35.2749352325856	37.0	35.0	37.0	33.0	37.0
9	37.351372323405585	39.0	37.0	39.0	34.0	39.0
10	36.90061065150958	39.0	37.0	39.0	33.0	39.0
11	36.86672384310926	39.0	37.0	39.0	33.0	39.0
12	37.170371593606895	39.0	37.0	39.0	34.0	39.0
13	37.10475896222981	39.0	37.0	39.0	33.0	39.0
14	38.105305867332895	40.0	37.0	41.0	33.0	41.0
15	38.18943869424631	40.0	37.0	41.0	33.0	41.0
16	38.23395463883843	40.0	38.0	41.0	33.0	41.0
17	38.089678231578795	40.0	37.0	41.0	33.0	41.0
18	37.95492116931152	39.0	37.0	40.0	33.0	41.0
19	37.74578501302451	39.0	37.0	41.0	33.0	41.0
20	37.40167516322809	39.0	35.0	40.0	33.0	41.0
21	37.41385623143319	39.0	35.0	40.0	33.0	41.0
22	37.402298421965696	39.0	35.0	40.0	33.0	41.0
23	37.05862005898762	39.0	35.0	40.0	32.0	41.0
24	36.82889860770035	39.0	35.0	40.0	32.0	41.0
25	36.638682171781475	38.0	35.0	40.0	31.0	41.0
26	36.88921265197481	39.0	35.0	40.0	32.0	41.0
27	36.812795093479934	39.0	35.0	40.0	32.0	41.0
28	36.88289305696869	39.0	35.0	40.0	32.0	41.0
29	36.903755355852326	39.0	35.0	40.0	32.0	41.0
30	36.622504079592446	39.0	35.0	40.0	31.0	41.0
31	36.422136161614006	39.0	35.0	40.0	31.0	41.0
32	36.061370565496325	39.0	35.0	40.0	30.0	41.0
33	35.88320379850453	39.0	35.0	40.0	28.0	41.0
34	35.49746523690047	39.0	35.0	40.0	25.0	41.0
35	35.284616163176594	39.0	35.0	40.0	24.0	41.0
36	35.16647400691444	39.0	35.0	40.0	23.0	41.0
37	35.10482466186882	39.0	35.0	40.0	22.0	41.0
38	35.11390009038139	39.0	35.0	40.0	22.0	41.0
39	34.891149903492554	39.0	35.0	40.0	21.0	41.0
40	34.66690105456799	38.0	35.0	40.0	20.0	41.0
41	34.48064080231689	38.0	34.0	40.0	19.0	41.0
42	34.65931008276379	38.0	35.0	40.0	20.0	41.0
43	34.66846719190865	39.0	35.0	40.0	18.0	41.0
44	34.65177415660308	39.0	35.0	40.0	18.0	41.0
45	34.61928834861294	38.0	35.0	40.0	20.0	41.0
46	34.41509209491289	38.0	34.0	40.0	20.0	41.0
47	34.27617287173122	38.0	34.0	40.0	18.0	41.0
48	34.264437495671814	38.0	34.0	40.0	18.0	41.0
49	34.30206385649778	38.0	34.0	40.0	18.0	41.0
50	34.1756684050436	38.0	34.0	40.0	18.0	41.0
51	33.27901216153588	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	11.0
11	14.0
12	18.0
13	19.0
14	31.0
15	39.0
16	71.0
17	137.0
18	268.0
19	538.0
20	1031.0
21	1777.0
22	2917.0
23	4267.0
24	5711.0
25	7773.0
26	10238.0
27	11663.0
28	11447.0
29	11069.0
30	11890.0
31	13129.0
32	16072.0
33	20764.0
34	29814.0
35	45452.0
36	59177.0
37	76722.0
38	116770.0
39	104300.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.11841028181594	21.798607522786234	22.319765470045404	20.763216725352425
2	36.03554172903693	28.234508646605192	23.732485275290365	11.997464349067508
3	24.59936537700051	27.57023202626565	36.483187107244895	11.347215489488946
4	22.502126359938135	23.303661955824985	40.36532550619796	13.828886178038918
5	21.899465346991757	30.825382789180512	32.70687839707086	14.568273466756871
6	19.768311110874357	35.2965095735028	33.33795006472302	11.597229250899819
7	62.0889999271977	6.999675053136803	26.408591381983026	4.502733637682472
8	60.07699287425267	11.108921123144206	22.96379949890708	5.850286503696048
9	53.94526332237748	10.450681766929643	24.678915210176697	10.925139700516185
10	21.882951653944023	37.29129266703246	29.435036374516354	11.390719304507172
11	21.49514621721011	23.667140769467068	37.67394867260094	17.16376434072188
12	20.330842074048817	20.260525703652014	40.35840040911343	19.05023181318574
13	21.03098714595441	21.235543859836035	43.06682363553392	14.666645358675638
14	14.726662866741599	27.342769222027492	39.667133666803394	18.26343424442752
15	12.460913153955563	26.265472708902653	43.613551172028295	17.66006296511349
16	14.011602201115473	26.620428326133005	38.6399109325975	20.72805854015402
17	13.83208237669332	26.96721588013545	39.516557196862756	19.68414454630848
18	12.462333686690851	26.46736592390561	41.91388375425494	19.156416635148595
19	13.597516908778715	26.451740063817432	40.18420758244861	19.766535444955245
20	14.940985743178336	28.16650064190323	42.072983420607315	14.81953019431112
21	15.534235726753426	28.080558411418245	40.67002977791746	15.715176083910867
22	12.970351706148598	25.09513130161639	40.079088160037216	21.855428832197795
23	13.786270195980247	26.272752939171014	40.17674978558834	19.7642270792604
24	15.411359645150924	25.12354195632217	38.424167523425474	21.040930875101434
25	12.71341284765319	30.753645886048414	36.24347220816487	20.289469058133527
26	11.9207555813619	29.289964468924957	39.11756506483844	19.6717148848747
27	15.385967622507632	27.03451361846977	39.18273200406983	18.396786754952778
28	11.032745055214331	27.46475747067044	39.66961959909015	21.83287787502508
29	16.009581493299525	25.880508337639323	37.784927792545396	20.324982376515752
30	15.976731673795966	28.612547920783992	37.824880275725405	17.58584012969464
31	15.417574475867813	29.91499887245214	32.54014336726631	22.12728328441374
32	17.547130612658012	31.74020587070666	31.707533617795015	19.005129898840313
33	18.36606773455215	26.425282641622676	32.08805882426057	23.120590799564606
34	15.911742301156492	30.43775491903851	31.275158966491407	22.375343813313588
35	16.208633642831902	30.31843016927423	31.88723100880908	21.585705179084787
36	15.011657246758967	30.80940179590851	31.9577249457978	22.221216011534725
37	16.31979032936827	28.511157396802734	34.66508277266682	20.50396950116217
38	19.419037624585158	27.547858635684847	29.035333976124395	23.997769763605596
39	23.097152009432335	26.7511173377796	29.90079354509925	20.25093710768881
40	19.625014871202072	29.979633111907795	31.861128719798142	18.53422329709199
41	15.526600363301245	32.85638946746003	28.902336598782956	22.71467357045576
42	15.691027027410955	29.574603715758503	32.67615937667024	22.058209880160305
43	19.48367186404081	24.965507689521264	31.56246171220362	23.988358734234303
44	19.50870875350028	25.036179193101894	31.55020961736175	23.904902436036075
45	20.547650882772313	23.978592571679194	30.397269736082777	25.07648680946572
46	21.195946509839853	28.23237784750226	30.978090058224083	19.593585584433804
47	15.886882978288932	27.705360202710022	36.762854489504925	19.64490232949612
48	17.139792850813876	26.63197015460723	33.02241423089694	23.20582276368195
49	18.842478900649716	23.3677634955049	36.058270252801556	21.731487351043825
50	19.081483533362096	22.80558766551426	33.349491893197246	24.763436907926394
51	17.854853516440002	22.59375072136428	30.640358400409113	28.911037361786608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4569.0
1	6812.0
2	9055.0
3	18935.5
4	28816.0
5	18587.0
6	8358.0
7	7881.5
8	7405.0
9	7601.0
10	7797.0
11	7605.0
12	7413.0
13	7147.5
14	6882.0
15	6746.0
16	6610.0
17	6014.5
18	5419.0
19	5539.5
20	5660.0
21	6367.0
22	7074.0
23	6756.0
24	6438.0
25	7510.0
26	9148.0
27	9714.0
28	9912.5
29	10111.0
30	10046.5
31	9982.0
32	10660.0
33	11338.0
34	11804.5
35	12271.0
36	13178.5
37	14086.0
38	14966.0
39	15846.0
40	18100.5
41	20355.0
42	23906.5
43	27458.0
44	31165.0
45	34872.0
46	50469.0
47	66066.0
48	64542.0
49	63018.0
50	59156.5
51	55295.0
52	43012.5
53	30730.0
54	24121.5
55	17513.0
56	14642.0
57	11771.0
58	10508.0
59	9245.0
60	8871.5
61	8498.0
62	7316.0
63	6134.0
64	4935.5
65	3737.0
66	2813.5
67	1890.0
68	1639.0
69	1388.0
70	1071.0
71	754.0
72	628.0
73	502.0
74	367.0
75	180.0
76	128.0
77	111.0
78	94.0
79	62.0
80	30.0
81	22.0
82	14.0
83	11.0
84	8.0
85	7.5
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.842708278169425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47951309041142	30.01102317573605
2	10.706928699411597	8.745999308504556
3	4.5578159667458085	5.584606437461455
4	2.5700275319697066	4.198675390204098
5	1.7855324993888917	3.6462991496865627
6	1.328860370429633	3.256455387112659
7	1.0266776070330506	2.9352605799845963
8	0.8091724422668897	2.6439035204992374
9	0.6814837285760111	2.5050277010294297
>10	2.9153888861045765	18.279766958407915
>50	0.07655954576008465	2.172476111948278
>100	0.04927970761035102	3.679857171039056
>500	0.004399973893781341	1.3736791627397835
>1k	0.007039958230050146	5.929966642083643
>5k	8.799947787562683E-4	1.8288766142337487
>10k+	4.3999738937813414E-4	3.2081266893289655
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17852	3.1699187987975193	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	5092	0.9041690860114814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	5085	0.9029261198681036	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	4354	0.7731249411810664	RNA PCR Primer, Index 30 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	3641	0.6465199611484297	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	3336	0.5923621506155345	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2803	0.4977191571269015	RNA PCR Primer, Index 30 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2793	0.49594349120779024	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2074	0.3682731116236867	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	0.3432362221642171	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	1855	0.3293860279951489	RNA PCR Primer, Index 30 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	1701	0.30204077284083464	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1413	0.25090159437042875	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1326	0.2354533008741603	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.21521070939629133	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	1208	0.21450044302864682	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.21023884482277966	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	1148	0.20384644751397893	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.18058522397362073	No Hit
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	992	0.17614605917584242	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	889	0.15785670020899586	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	887	0.15750156702517362	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	860	0.15270726904357307	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	844	0.14986620357299496	No Hit
GATAGAGGTTTTTTAGGATTAAGGATAAGCTAATTTTATTAAATGAAAATA	841	0.14933350379726157	No Hit
ACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	648	0.11506315155841318	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	585	0.10387645626801191	RNA PCR Primer, Index 30 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	566	0.10050269102170042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5513318382226296E-4	0.0	0.0	0.360637748171508	0.0
2	3.5513318382226296E-4	0.0	0.0	1.8575241179823463	0.0
3	3.5513318382226296E-4	0.0	0.0	2.4477554694949473	0.0
4	3.5513318382226296E-4	0.0	0.0	3.353877788017451	0.0
5	3.5513318382226296E-4	0.0	0.0	6.154813208823639	0.0
6	3.5513318382226296E-4	0.0	0.0	7.366350065433289	0.0
7	3.5513318382226296E-4	0.0	0.0	8.698987337726331	0.0
8	3.5513318382226296E-4	0.0	0.0	10.34059047994474	0.0
9	3.5513318382226296E-4	0.0	0.0	10.914308138409607	0.0
10	3.5513318382226296E-4	0.0	0.0	13.729271319976775	0.0
11	3.5513318382226296E-4	0.0	0.0	16.944824732895455	0.0
12	3.5513318382226296E-4	0.0	0.0	20.37008429086118	0.0
13	3.5513318382226296E-4	0.0	0.0	21.38718572932814	0.0
14	3.5513318382226296E-4	0.0	0.0	21.823821978837614	0.0
15	3.5513318382226296E-4	0.0	0.0	22.819260293091418	0.0
16	3.5513318382226296E-4	0.0	0.0	24.47027446468112	0.0
17	3.5513318382226296E-4	0.0	0.0	26.46523512480268	0.0
18	3.5513318382226296E-4	0.0	0.0	28.27463869637711	0.0
19	3.5513318382226296E-4	0.0	0.0	29.88055095362138	0.0
20	3.5513318382226296E-4	0.0	0.0	31.331270009535327	0.0
21	3.5513318382226296E-4	0.0	0.0	32.82797881275425	0.0
22	3.5513318382226296E-4	0.0	0.0	34.485207815060846	0.0
23	3.5513318382226296E-4	0.0	0.0	35.83382608062589	0.0
24	3.5513318382226296E-4	0.0	0.0	36.733023302063856	0.0
25	3.5513318382226296E-4	0.0	0.0	37.47099005804652	0.0
26	3.5513318382226296E-4	0.0	0.0	38.09780012749281	0.0
27	3.5513318382226296E-4	0.0	0.0	38.660508657259186	0.0
28	3.5513318382226296E-4	0.0	0.0	39.20954455944841	0.0
29	3.5513318382226296E-4	0.0	0.0	39.76674852486554	0.0
30	3.5513318382226296E-4	0.0	0.0	40.3578677093377	0.0
31	3.5513318382226296E-4	0.0	0.0	40.84013857296833	0.0
32	3.5513318382226296E-4	0.0	0.0	41.31015734175709	0.0
33	3.5513318382226296E-4	0.0	0.0	41.742354426468786	0.0
34	3.5513318382226296E-4	0.0	0.0	42.13601956073577	0.0
35	3.5513318382226296E-4	0.0	0.0	42.58348737235182	0.0
36	3.5513318382226296E-4	0.0	0.0	42.968629310207064	0.0
37	3.5513318382226296E-4	0.0	0.0	43.38005110366515	0.0
38	3.5513318382226296E-4	0.0	0.0	43.77282840497257	0.0
39	3.5513318382226296E-4	0.0	0.0	44.139858550452885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCG	35	1.2104465E-7	45.0	7
TTCTACG	20	7.030219E-4	45.0	1
CGGCGAA	25	3.888263E-5	45.0	31
TCCGCTA	35	1.2104465E-7	45.0	2
GATCGAC	25	3.888263E-5	45.0	10
CGACGGT	50	2.1827873E-11	45.0	28
CTATACG	20	7.030219E-4	45.0	1
GACCGTC	40	6.8048394E-9	45.0	9
CGGTTAG	20	7.030219E-4	45.0	1
GGCGTAT	40	6.8048394E-9	45.0	7
CAGCACG	20	7.030219E-4	45.0	1
ATCCGCT	35	1.2104465E-7	45.0	1
AACGGGC	25	3.888263E-5	45.0	4
CACTCGA	20	7.030219E-4	45.0	41
TGACCGG	25	3.888263E-5	45.0	2
CGCTAGT	35	1.2104465E-7	45.0	4
GCGATCG	35	1.2104465E-7	45.0	33
TCGTAAG	20	7.030219E-4	45.0	1
GGTTGCG	20	7.030219E-4	45.0	1
ACCGTCT	25	3.888263E-5	45.0	10
>>END_MODULE