FastQCFastQC Report
Sat 14 Jan 2017
SRR2935200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087938
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233742.1484680193172774No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC65660.6035270392246617TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC54890.5045324273993554TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC52820.48550560785633007No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT41980.3858675770126606TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36900.3391737396800185No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC32550.2991898435388781TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC19210.1765725620393809TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG16860.1549720664229028No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.14173601804514596No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.1323604837775682No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13190.12123852646014753No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC13020.11967593741555124No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.11746992935259178No Hit
ACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG12540.11526392128963232TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG12140.11158724118469987TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC12020.11048423715322013No Hit
AGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG11170.10267129193023868TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC11080.10184403890662888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC207.0331205E-445.030
AGTCACG253.890668E-545.01
TTAGCGT207.0331205E-445.030
CTATACG207.0331205E-445.01
TAGCGCG207.0331205E-445.01
TAGCGAA207.0331205E-445.01
AATATCG253.890668E-545.017
TGCGAAG502.1827873E-1145.01
TTACGCG253.890668E-545.01
GGTTACG253.890668E-545.01
CGCATCG302.165394E-644.99999621
CGGTCTA1350.043.33333631
CGTTATT5800.043.0603451
CGTTTTT80100.043.033711
ACGCATG1100.042.9545441
CGTTAGG1550.042.0967752
CGACGGT1450.041.8965528
CGTTTTC1350.041.6666681
GCGTTAG650.041.5384641
ACGTTAG603.6379788E-1241.2499961
Produced by FastQC (version 0.11.5)