##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087938
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.544690046675456	31.0	31.0	34.0	30.0	34.0
2	31.65234691682798	31.0	31.0	34.0	30.0	34.0
3	31.664654603479242	31.0	31.0	34.0	30.0	34.0
4	35.490302756223244	37.0	35.0	37.0	33.0	37.0
5	32.859913892151944	37.0	35.0	37.0	28.0	37.0
6	33.90634760436716	36.0	35.0	37.0	26.0	37.0
7	35.3167239309593	37.0	35.0	37.0	32.0	37.0
8	35.30545306809763	37.0	35.0	37.0	33.0	37.0
9	37.34175752662376	39.0	37.0	39.0	34.0	39.0
10	36.68194786835279	39.0	35.0	39.0	32.0	39.0
11	36.64816469320862	39.0	37.0	39.0	32.0	39.0
12	36.98708658030145	39.0	37.0	39.0	33.0	39.0
13	36.93713060854571	39.0	37.0	39.0	33.0	39.0
14	37.79794528732336	39.0	37.0	41.0	33.0	41.0
15	37.94554836764595	39.0	37.0	41.0	33.0	41.0
16	37.936894381848965	40.0	37.0	41.0	33.0	41.0
17	37.914286475883735	39.0	37.0	41.0	33.0	41.0
18	37.84908515007289	39.0	37.0	40.0	33.0	41.0
19	37.6893692471446	39.0	37.0	40.0	33.0	41.0
20	37.52938402739862	39.0	35.0	40.0	33.0	41.0
21	37.451101073774424	39.0	35.0	40.0	33.0	41.0
22	37.473601436846586	39.0	35.0	40.0	33.0	41.0
23	37.18893631806224	39.0	35.0	40.0	32.0	41.0
24	36.93428761565457	39.0	35.0	40.0	32.0	41.0
25	36.74801780983843	39.0	35.0	40.0	31.0	41.0
26	37.09689246997531	39.0	35.0	40.0	32.0	41.0
27	37.01870970588398	39.0	35.0	40.0	32.0	41.0
28	37.11076090733112	39.0	35.0	40.0	32.0	41.0
29	37.210646194911845	39.0	35.0	40.0	33.0	41.0
30	36.957608797560155	39.0	35.0	40.0	32.0	41.0
31	36.82383463028224	39.0	35.0	40.0	31.0	41.0
32	36.62279376214453	39.0	35.0	40.0	31.0	41.0
33	36.53427493110821	39.0	35.0	40.0	31.0	41.0
34	36.44497756305966	39.0	35.0	40.0	30.0	41.0
35	36.28655125567817	39.0	35.0	40.0	30.0	41.0
36	36.19675937415551	39.0	35.0	40.0	30.0	41.0
37	36.11250641121093	39.0	35.0	40.0	29.0	41.0
38	36.00164439517693	39.0	35.0	40.0	29.0	41.0
39	35.93450453978076	39.0	35.0	40.0	28.0	41.0
40	35.86439668436988	39.0	35.0	40.0	27.0	41.0
41	35.78727831916893	39.0	35.0	40.0	27.0	41.0
42	35.88978416049444	39.0	35.0	40.0	27.0	41.0
43	35.79174640466644	39.0	35.0	40.0	26.0	41.0
44	35.682003937724396	39.0	35.0	40.0	26.0	41.0
45	35.66299090573176	39.0	35.0	40.0	25.0	41.0
46	35.575432607372846	39.0	35.0	40.0	25.0	41.0
47	35.50345240261853	39.0	35.0	40.0	24.0	41.0
48	35.469623268973045	39.0	35.0	40.0	24.0	41.0
49	35.49270914335192	39.0	35.0	40.0	24.0	41.0
50	35.424210754656976	39.0	35.0	40.0	24.0	41.0
51	34.57646299697225	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	18.0
11	30.0
12	25.0
13	33.0
14	46.0
15	80.0
16	133.0
17	254.0
18	465.0
19	757.0
20	1314.0
21	2239.0
22	3522.0
23	5248.0
24	7998.0
25	11513.0
26	14978.0
27	16708.0
28	17279.0
29	17782.0
30	20140.0
31	24422.0
32	30480.0
33	41448.0
34	61059.0
35	82787.0
36	112804.0
37	157229.0
38	247056.0
39	210025.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.067991006840465	22.86196456048047	24.046682807292328	18.02336162538674
2	35.49568082004673	23.465675433710377	25.552559061270035	15.486084684972855
3	27.47886368524677	23.771483301438135	34.95098066250099	13.798672350814108
4	24.62971235493199	25.200700775228	33.828490226465114	16.341096643374897
5	19.251188946428933	32.77576479542033	31.76385051354029	16.209195744610447
6	21.520527824195863	32.77962530953051	32.80618932328864	12.893657542984988
7	69.48199254001607	6.457261351290239	18.756307804305024	5.304438304388669
8	67.1916046686484	6.0538376267765255	19.21396256036649	7.540595144208585
9	61.177199435997274	9.326634422182147	21.916506271497088	7.579659870323492
10	23.003792495528238	37.51969321781205	26.849324134279712	12.627190152380008
11	19.378585912064842	23.280738424432272	37.50425116137133	19.836424502131557
12	19.965751724822553	20.115668356101175	40.75213844906603	19.16644147001024
13	20.44417972347689	21.665389020330203	42.73469627864823	15.155734977544677
14	15.75236824157259	25.111449365680766	39.8092538361561	19.32692855659054
15	12.94844007654848	25.64420031288548	43.18720368256279	18.22015592800325
16	14.363778082942227	26.804836305010028	38.24887079962277	20.582514812424975
17	14.244469813537169	26.542689013528342	36.879858962551175	22.332982210383314
18	13.949875820129456	25.334531931047543	41.70927019738257	19.00632205144043
19	14.357527726763841	27.163404532243568	38.67260818171624	19.806459559276355
20	14.171120045443766	28.914423432217646	38.896426083104004	18.01803043923459
21	14.701021565567155	27.37619239331653	40.136294531489845	17.78649150962647
22	12.891359617919404	24.420233505953465	37.6350490561043	25.053357820022832
23	12.380117249328547	25.538128091858177	39.05792425671316	23.02383040210012
24	15.280925935117626	24.209008233925093	37.073344253073245	23.436721577884036
25	13.050743700468226	27.643119368934627	34.960080445760696	24.34605648483645
26	12.09931080631433	26.82864280868947	37.40295862448043	23.669087760515765
27	15.15555114353943	25.31366677145205	38.28416692863013	21.246615156378397
28	12.038185999569828	25.500993622798358	37.58651687871919	24.874303498912624
29	13.924230976397553	24.001735393009525	35.706262673056735	26.367770957536184
30	16.780459915914324	25.318630289593706	35.210646194911845	22.690263599580124
31	16.11553231893729	28.515871308842968	30.895510589757873	24.47308578246187
32	14.497793072767015	29.275381501519387	31.324762992008736	24.90206243370486
33	15.235702769826956	25.024128213188618	32.87117464414332	26.868994372841097
34	15.201693478856331	23.08192194775805	31.487915671665114	30.2284689017205
35	12.5404205019036	24.437973487459764	33.28884550406365	29.732760506572987
36	15.837942971014893	23.106371870455853	35.6387036761286	25.41698148240065
37	17.181769549367704	25.60853651586763	35.214966294035136	21.99472764072953
38	16.315359882640372	27.486400879461883	32.13850421623291	24.05973502166484
39	17.64328481953935	22.89873136152979	31.568710716971005	27.889273101959855
40	17.049684816598003	20.60494256106506	33.9818077868408	28.363564835496142
41	15.320634080250898	21.499570747597748	34.443231140009814	28.736564032141537
42	17.77316354424609	20.73950905290559	38.64797442501319	22.839352977835134
43	19.669319391362375	20.99963417032956	36.51972814627304	22.811318292035025
44	18.98996082497348	21.754180844864322	30.563322542277227	28.69253578788497
45	19.178298763348646	19.24061849112725	30.444014272872167	31.137068472651936
46	20.575345286220355	19.925859745684036	33.34417953964288	26.154615428452725
47	15.07668635528863	21.118574771724123	39.16059554864339	24.64414332434385
48	15.896402184683318	20.59382060374764	37.84848033619563	25.661296875373413
49	19.39329263248457	17.971336601901946	38.31891155562174	24.316459209991745
50	17.609091694563478	16.751781811095853	36.15968924699753	29.47943724734314
51	16.58881296544472	16.396798346964626	32.76785993319472	34.24652875439593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5242.0
1	7238.5
2	9235.0
3	23651.0
4	38067.0
5	24197.0
6	10327.0
7	10017.5
8	9708.0
9	10047.5
10	10387.0
11	10392.5
12	10398.0
13	10208.5
14	10019.0
15	9392.5
16	8766.0
17	8724.0
18	8682.0
19	8696.5
20	8711.0
21	8584.0
22	8457.0
23	9113.5
24	9770.0
25	10707.5
26	12421.0
27	13197.0
28	14052.5
29	14908.0
30	16168.0
31	17428.0
32	18431.5
33	19435.0
34	20607.0
35	21779.0
36	23293.5
37	24808.0
38	26600.0
39	28392.0
40	32339.5
41	36287.0
42	44962.0
43	53637.0
44	65277.5
45	76918.0
46	100019.5
47	123121.0
48	142627.0
49	162133.0
50	153120.0
51	144107.0
52	112283.5
53	80460.0
54	60557.0
55	40654.0
56	31305.5
57	21957.0
58	18561.5
59	15166.0
60	13155.0
61	11144.0
62	9861.5
63	8579.0
64	7109.0
65	5639.0
66	4427.5
67	3216.0
68	2716.5
69	2217.0
70	1800.0
71	1383.0
72	1100.0
73	817.0
74	664.5
75	414.0
76	316.0
77	239.0
78	162.0
79	121.5
80	81.0
81	60.5
82	40.0
83	25.0
84	10.0
85	11.0
86	12.0
87	7.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1087938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.698710556141755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.88128372601336	24.911555836403004
2	12.227071484373988	8.97435514708591
3	5.70639209420979	6.2825169535578205
4	3.328883708741747	4.886629588086763
5	2.2200557995490593	4.07365926030674
6	1.6058193627250492	3.5358899998856765
7	1.2529969725228602	3.2188361255636853
8	0.990949450750654	2.909325365509272
9	0.8003447386598013	2.643445791828627
>10	3.7636290329933644	22.208473531751537
>50	0.1412660773827765	3.5507746596831438
>100	0.06947164266011978	4.583186579520167
>500	0.006432559465956177	1.5849514073728483
>1k	0.0043741404368502	2.7921961939836244
>5k	7.719071359147413E-4	1.6370749210380144
>10k+	2.573023786382471E-4	2.2071286384231734
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23374	2.1484680193172774	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	6566	0.6035270392246617	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	5489	0.5045324273993554	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	5282	0.48550560785633007	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	4198	0.3858675770126606	TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3690	0.3391737396800185	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3255	0.2991898435388781	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1921	0.1765725620393809	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	1686	0.1549720664229028	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1542	0.14173601804514596	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1440	0.1323604837775682	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1319	0.12123852646014753	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	1302	0.11967593741555124	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1278	0.11746992935259178	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1254	0.11526392128963232	TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1214	0.11158724118469987	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	1202	0.11048423715322013	No Hit
AGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1117	0.10267129193023868	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1108	0.10184403890662888	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.191700262331126E-5	0.0	0.0	0.4155567688599902	0.0
2	9.191700262331126E-5	0.0	0.0	2.1003954269452856	0.0
3	9.191700262331126E-5	0.0	0.0	2.871303327946997	0.0
4	9.191700262331126E-5	0.0	0.0	3.9919554239304076	0.0
5	9.191700262331126E-5	0.0	0.0	7.06161564353851	0.0
6	9.191700262331126E-5	0.0	0.0	8.860523301879335	0.0
7	9.191700262331126E-5	0.0	0.0	10.692245330156682	0.0
8	9.191700262331126E-5	0.0	0.0	13.625500717871791	0.0
9	9.191700262331126E-5	0.0	0.0	14.603129957773328	0.0
10	9.191700262331126E-5	0.0	0.0	17.623798414983206	0.0
11	9.191700262331126E-5	0.0	0.0	22.120653934323464	0.0
12	1.8383400524662252E-4	0.0	0.0	25.71672282795527	0.0
13	1.8383400524662252E-4	0.0	0.0	27.084263992984894	0.0
14	1.8383400524662252E-4	0.0	0.0	27.61664727217911	0.0
15	1.8383400524662252E-4	0.0	0.0	28.71137877342275	0.0
16	1.8383400524662252E-4	0.0	0.0	30.835856455055342	0.0
17	1.8383400524662252E-4	0.0	0.0	33.64759756530243	0.0
18	2.757510078699338E-4	0.0	0.0	36.41558618230083	0.0
19	2.757510078699338E-4	0.0	0.0	38.16154964713063	0.0
20	2.757510078699338E-4	0.0	0.0	40.12167972807274	0.0
21	2.757510078699338E-4	0.0	0.0	42.364822260092026	0.0
22	2.757510078699338E-4	0.0	0.0	44.71679452321732	0.0
23	2.757510078699338E-4	0.0	0.0	46.5983355669165	0.0
24	2.757510078699338E-4	0.0	0.0	47.841880695407276	0.0
25	2.757510078699338E-4	0.0	0.0	48.78421380630146	0.0
26	2.757510078699338E-4	0.0	0.0	49.588395662252815	0.0
27	2.757510078699338E-4	0.0	0.0	50.330349707428184	0.0
28	2.757510078699338E-4	0.0	0.0	51.05741319817857	0.0
29	2.757510078699338E-4	0.0	0.0	51.74054036167502	0.0
30	2.757510078699338E-4	0.0	0.0	52.48010456478218	0.0
31	2.757510078699338E-4	0.0	0.0	53.092547553261305	0.0
32	2.757510078699338E-4	0.0	0.0	53.59340330055573	0.0
33	2.757510078699338E-4	0.0	0.0	54.0372705062237	0.0
34	2.757510078699338E-4	0.0	0.0	54.475163106721155	0.0
35	2.757510078699338E-4	0.0	0.0	54.92923309968031	0.0
36	2.757510078699338E-4	0.0	0.0	55.341297022440614	0.0
37	2.757510078699338E-4	0.0	0.0	55.72753226746377	0.0
38	2.757510078699338E-4	0.0	0.0	56.132150913011586	0.0
39	2.757510078699338E-4	0.0	0.0	56.5820846408527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	20	7.0331205E-4	45.0	30
AGTCACG	25	3.890668E-5	45.0	1
TTAGCGT	20	7.0331205E-4	45.0	30
CTATACG	20	7.0331205E-4	45.0	1
TAGCGCG	20	7.0331205E-4	45.0	1
TAGCGAA	20	7.0331205E-4	45.0	1
AATATCG	25	3.890668E-5	45.0	17
TGCGAAG	50	2.1827873E-11	45.0	1
TTACGCG	25	3.890668E-5	45.0	1
GGTTACG	25	3.890668E-5	45.0	1
CGCATCG	30	2.165394E-6	44.999996	21
CGGTCTA	135	0.0	43.333336	31
CGTTATT	580	0.0	43.060345	1
CGTTTTT	8010	0.0	43.03371	1
ACGCATG	110	0.0	42.954544	1
CGTTAGG	155	0.0	42.096775	2
CGACGGT	145	0.0	41.89655	28
CGTTTTC	135	0.0	41.666668	1
GCGTTAG	65	0.0	41.538464	1
ACGTTAG	60	3.6379788E-12	41.249996	1
>>END_MODULE