##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935199.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1820186
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07764700970121	33.0	31.0	34.0	30.0	34.0
2	32.21506153766703	34.0	31.0	34.0	30.0	34.0
3	32.12999990110901	33.0	31.0	34.0	30.0	34.0
4	35.83716609181699	37.0	35.0	37.0	35.0	37.0
5	33.30378708549566	37.0	35.0	37.0	30.0	37.0
6	34.370259413049	37.0	35.0	37.0	28.0	37.0
7	35.66339483986801	37.0	35.0	37.0	32.0	37.0
8	35.665666585722555	37.0	35.0	37.0	35.0	37.0
9	37.7987974855317	39.0	38.0	39.0	35.0	39.0
10	37.365047308352004	39.0	37.0	39.0	34.0	39.0
11	37.259111431469094	39.0	37.0	39.0	34.0	39.0
12	37.439931413602785	39.0	37.0	39.0	35.0	39.0
13	37.39361746546781	39.0	37.0	39.0	35.0	39.0
14	38.48594154663315	40.0	38.0	41.0	34.0	41.0
15	38.6348038057649	40.0	38.0	41.0	34.0	41.0
16	38.61506461427568	40.0	38.0	41.0	34.0	41.0
17	38.54176935763708	40.0	38.0	41.0	34.0	41.0
18	38.18283296322464	39.0	38.0	41.0	34.0	41.0
19	37.720196177753266	39.0	37.0	41.0	34.0	41.0
20	37.254038872950346	39.0	35.0	40.0	34.0	41.0
21	37.13073993536924	38.0	35.0	40.0	33.0	41.0
22	37.101824209174225	38.0	35.0	40.0	33.0	41.0
23	36.912133155622556	38.0	35.0	40.0	33.0	41.0
24	36.62972080875251	38.0	35.0	40.0	33.0	41.0
25	36.42075699955939	38.0	35.0	40.0	32.0	41.0
26	36.655306106079266	38.0	35.0	40.0	33.0	41.0
27	36.563752275866314	38.0	35.0	40.0	33.0	41.0
28	36.531939043592246	38.0	35.0	40.0	33.0	41.0
29	36.470564546700174	38.0	35.0	40.0	33.0	41.0
30	36.08867390475479	38.0	35.0	40.0	31.0	41.0
31	35.72031759391623	38.0	35.0	40.0	30.0	41.0
32	35.08855468616944	38.0	35.0	40.0	24.0	41.0
33	34.43001759160877	38.0	34.0	40.0	18.0	41.0
34	33.83138701209656	38.0	34.0	40.0	15.0	41.0
35	33.37396343011099	38.0	33.0	40.0	10.0	41.0
36	33.09620610201375	38.0	33.0	40.0	10.0	41.0
37	33.00184321822056	38.0	33.0	40.0	10.0	41.0
38	32.86105705680628	38.0	33.0	40.0	10.0	41.0
39	32.69992901824319	38.0	32.0	40.0	10.0	41.0
40	32.600074388002106	37.0	32.0	40.0	10.0	41.0
41	32.470112395106874	37.0	31.0	40.0	8.0	41.0
42	32.4522587252072	37.0	32.0	40.0	8.0	41.0
43	32.33674360752143	37.0	31.0	40.0	8.0	41.0
44	32.20922202456232	37.0	31.0	40.0	8.0	41.0
45	32.163838201150874	37.0	31.0	40.0	8.0	41.0
46	32.07055322917548	36.0	31.0	40.0	8.0	41.0
47	31.98259024077759	36.0	31.0	40.0	8.0	41.0
48	31.874962229134823	36.0	30.0	40.0	8.0	41.0
49	31.80798445873114	36.0	30.0	40.0	8.0	41.0
50	31.695433323847123	36.0	30.0	40.0	8.0	41.0
51	30.9218590847309	35.0	27.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	37.0
10	44.0
11	58.0
12	66.0
13	96.0
14	136.0
15	194.0
16	365.0
17	691.0
18	1278.0
19	2281.0
20	3901.0
21	6233.0
22	9519.0
23	15510.0
24	26605.0
25	45036.0
26	64814.0
27	69470.0
28	61863.0
29	52504.0
30	46661.0
31	46594.0
32	51184.0
33	64212.0
34	93184.0
35	129606.0
36	163007.0
37	188241.0
38	300872.0
39	375763.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.085339630125713	15.130376785669156	18.60178025762202	35.18250332658311
2	49.882319718973775	17.880425407073783	20.05729084829792	12.179964025654522
3	23.440241821440228	18.170450712179964	46.901745206259136	11.487562260120669
4	20.65255968346092	20.126789240220504	46.39278623173676	12.827864844581818
5	17.13764417482609	28.470881547270444	42.39929325904056	11.992181018862908
6	19.535915560277907	26.091948844788387	43.881943933202436	10.490191661731274
7	59.885693000605436	5.2071052079293	31.891575915867936	3.015625875597329
8	60.87658074504474	4.6265052033143865	31.530294156751015	2.9666198948898628
9	55.25391361102656	8.125708032036288	32.79126418948393	3.829114167453216
10	20.737660876415926	33.927246995636715	36.913370391817104	8.421721736130264
11	20.37769766386512	20.849023121812827	45.8023520673162	12.970927147005856
12	20.021580212132168	19.026242373032208	46.86493578128829	14.08724163354734
13	19.107332986848597	18.49882374658414	49.12184798696397	13.271995279603294
14	14.456379732620734	21.238763510981844	49.504061672818054	14.800795083579372
15	13.597676281434975	22.344914201076154	49.86770582786594	14.18970368962293
16	17.049026857694763	22.77937529461275	47.23506279028627	12.936535057406221
17	16.80537044016381	22.277778205084537	46.80384312372472	14.113008231026939
18	15.983036898426864	21.784037455512788	48.63915006488348	13.593775581176867
19	15.847830935959292	22.682901637524957	47.82505743918479	13.644209987330965
20	15.648840283355659	24.357785413139098	47.2952214773655	12.698152826139747
21	16.379260141545974	22.14636306399456	49.15882223025559	12.315554564203877
22	16.0475907407265	20.189310323230703	48.861105403513704	14.901993532529092
23	13.934564929078677	21.846173962441203	49.160305595142475	15.058955513337649
24	14.142785407645153	22.292721732833897	48.060692698438515	15.50380016108244
25	14.982864388584463	23.82608151035114	46.179895900748605	15.01115820031579
26	14.578674926628377	23.791689420751506	46.12451694497155	15.50511870764856
27	14.554446633475921	22.521544501496003	47.85104379442541	15.072965070602676
28	13.606686349636796	22.972322608788332	47.39971629273052	16.02127474884435
29	15.502756311717594	22.694109283336978	46.60919268690123	15.193941718044199
30	16.93848870390169	21.985500382927896	46.077983239075564	14.998027674094846
31	16.688239553540132	23.56385556201399	44.48210237854813	15.26580250589775
32	17.38761862798637	24.05979388919594	43.558515448421204	14.994072034396485
33	17.621715582912955	23.618685123388488	42.03586886175369	16.723730431944865
34	16.670933629859803	25.18775553707149	41.58256354020963	16.55874729285908
35	17.44772237562535	26.472019892472527	39.44217788731481	16.63807984458731
36	19.08947766876572	26.673867395969424	38.372342167229064	15.864312768035793
37	18.498549049382866	27.421703056720574	38.3332252857675	15.74652260812906
38	17.869602337343547	27.3736859859377	38.21999509940193	16.536716577316824
39	17.577214636306397	26.428068340268524	37.635549333969166	18.359167689455912
40	18.648204084637506	26.394500342272714	38.128795628578615	16.828499944511165
41	17.91157606969837	27.154642437640987	37.43397652767354	17.499804964987096
42	18.331313393246624	27.11508604065738	37.73553911523328	16.818061450862714
43	18.482726490589425	26.16001881126434	38.058473145052204	17.298781553094024
44	19.02574791806991	25.349497249182228	37.02440300057247	18.600351832175395
45	18.74396352900198	24.703409431783346	37.19185841446973	19.360768624744942
46	18.630623463755903	25.398942745411734	37.648075526347306	18.322358264485057
47	17.017546558428645	26.471360619189465	39.15198776388787	17.359105058494023
48	17.04391748975105	26.103541066682194	38.99508072251957	17.85746072104719
49	18.25544202625446	24.85531698408844	38.994421449236505	17.894819540420595
50	16.572042637400795	24.80241030312287	39.211651996004804	19.413895063471536
51	16.716368546950697	24.581608692737994	38.17532933447461	20.5266934258367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8903.0
1	10107.0
2	11311.0
3	96638.0
4	181965.0
5	114003.0
6	46041.0
7	43584.0
8	41127.0
9	40485.0
10	39843.0
11	39106.0
12	38369.0
13	36686.0
14	35003.0
15	33538.5
16	32074.0
17	29878.0
18	27682.0
19	26584.5
20	25487.0
21	24368.5
22	23250.0
23	23372.5
24	23495.0
25	24949.5
26	26642.5
27	26881.0
28	30599.5
29	34318.0
30	36112.0
31	37906.0
32	42419.0
33	46932.0
34	50507.0
35	54082.0
36	54717.5
37	55353.0
38	58241.0
39	61129.0
40	65381.5
41	69634.0
42	74747.0
43	79860.0
44	83647.5
45	87435.0
46	96503.5
47	105572.0
48	114237.5
49	122903.0
50	121965.0
51	121027.0
52	106263.0
53	91499.0
54	78422.0
55	65345.0
56	59852.5
57	54360.0
58	48492.0
59	42624.0
60	37405.5
61	32187.0
62	28100.5
63	24014.0
64	20696.5
65	17379.0
66	14167.5
67	10956.0
68	9030.0
69	7104.0
70	5702.0
71	4300.0
72	3665.0
73	3030.0
74	2274.5
75	1175.0
76	831.0
77	645.5
78	460.0
79	359.5
80	259.0
81	203.0
82	147.0
83	112.0
84	77.0
85	56.0
86	35.0
87	45.5
88	56.0
89	32.5
90	9.0
91	7.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1820186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.68113683490883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33785064276928	25.274888793740846
2	8.185643342025838	5.350322602850137
3	3.2793766801839306	3.215212740549003
4	1.8746705379747826	2.4506545748770407
5	1.3038663807594835	2.1305917802019008
6	0.9415703696267947	1.846295405368138
7	0.7590244703477984	1.7364047803536422
8	0.6103599557515599	1.5957805785972505
9	0.5163710819322895	1.5188034587597166
>10	4.699703265959052	31.8161997253031
>50	0.4073594023517834	8.876641780336923
>100	0.07845082921037884	4.319190972579152
>500	0.0012203420530462836	0.27199636953521433
>1k	0.004009695317152074	2.0759806696176377
>5k	3.486691580132239E-4	0.6998180576039039
>10k+	1.7433457900661194E-4	6.821217709726435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119724	6.577569545090446	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6657	0.365731853777581	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCC	5626	0.30908929087466885	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	2614	0.14361169682658806	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2319	0.12740456195136102	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGT	2174	0.119438343114385	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTC	1974	0.10845045506338363	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	1960	0.10768130289981354	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTT	1945	0.10685721129598844	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	1916	0.10526396752859324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10213241943405783	0.0
2	0.0	0.0	0.0	0.40852967773623133	0.0
3	0.0	0.0	0.0	0.6477909400467864	0.0
4	0.0	0.0	0.0	0.9092477362203643	0.0
5	0.0	0.0	0.0	1.5712130518529426	0.0
6	0.0	0.0	0.0	2.1672510391795123	0.0
7	0.0	0.0	0.0	2.713019438672751	0.0
8	0.0	0.0	0.0	3.478270901984742	0.0
9	0.0	0.0	0.0	3.9119079039175118	0.0
10	0.0	0.0	0.0	4.7878623393433415	0.0
11	0.0	0.0	0.0	6.076302092203764	0.0
12	0.0	0.0	0.0	6.988681376518663	0.0
13	0.0	0.0	0.0	7.383695951952163	0.0
14	5.4939440255006906E-5	0.0	0.0	7.5999375887958704	0.0
15	5.4939440255006906E-5	0.0	0.0	7.890841924946132	0.0
16	5.4939440255006906E-5	0.0	0.0	8.395185986487096	0.0
17	5.4939440255006906E-5	0.0	0.0	8.99578394735483	0.0
18	5.4939440255006906E-5	0.0	0.0	9.607314856833312	0.0
19	5.4939440255006906E-5	0.0	0.0	10.029469515752785	0.0
20	5.4939440255006906E-5	0.0	0.0	10.523649780846572	0.0
21	5.4939440255006906E-5	0.0	0.0	11.038707033237262	0.0
22	5.4939440255006906E-5	0.0	0.0	11.598045474473487	0.0
23	5.4939440255006906E-5	0.0	0.0	12.062393623508806	0.0
24	5.4939440255006906E-5	0.0	0.0	12.388953656384567	0.0
25	5.4939440255006906E-5	0.0	0.0	12.663925554860876	0.0
26	5.4939440255006906E-5	0.0	0.0	12.927030534242105	0.0
27	5.4939440255006906E-5	0.0	0.0	13.210133469876155	0.0
28	5.4939440255006906E-5	0.0	0.0	13.462909834489443	0.0
29	5.4939440255006906E-5	0.0	0.0	13.719037504958285	0.0
30	5.4939440255006906E-5	0.0	0.0	14.009007870624211	0.0
31	5.4939440255006906E-5	0.0	0.0	14.245741918683036	0.0
32	5.4939440255006906E-5	0.0	0.0	14.487090879723281	0.0
33	5.4939440255006906E-5	0.0	0.0	14.708606702831469	0.0
34	5.4939440255006906E-5	0.0	0.0	14.934407802279548	0.0
35	5.4939440255006906E-5	0.0	0.0	15.157132293073346	0.0
36	5.4939440255006906E-5	0.0	0.0	15.375022113124702	0.0
37	1.0987888051001381E-4	0.0	0.0	15.59653793623289	0.0
38	1.6481832076502073E-4	0.0	0.0	15.861950372104829	0.0
39	1.6481832076502073E-4	0.0	0.0	16.2835006971815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAA	20	7.0343725E-4	45.000004	37
TATCGCG	40	6.8193913E-9	45.000004	1
CCCGATC	25	3.8917064E-5	45.0	41
CGTTTTT	43820	0.0	44.173325	1
CGTTATT	3415	0.0	44.143486	1
TCGATAG	80	0.0	42.187504	1
GATCGAC	115	0.0	41.086956	9
ACTACGG	105	0.0	40.714287	2
CGACGGT	205	0.0	39.512196	28
ACGGGTA	145	0.0	38.793106	5
TCACGAC	210	0.0	38.57143	25
CTTAACG	35	6.2503004E-6	38.571426	1
TATCACG	35	6.2503004E-6	38.571426	1
CGTAAGG	275	0.0	38.45454	2
GTTTTTT	51390	0.0	38.34939	2
GCGTACG	95	0.0	37.894737	1
GTTATTT	4110	0.0	37.883213	2
TAACGGG	455	0.0	37.582417	3
CACGACG	210	0.0	37.5	26
TCGCTAT	60	1.5643309E-10	37.499996	23
>>END_MODULE