SRR2935197.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400709
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9365	2.337107476997023	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4278	1.0676076654130553	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	3175	0.7923455674816388	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	3046	0.7601526294642746	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	2855	0.7124871165858516	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	2161	0.539294101205613	TruSeq Adapter, Index 27 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1620	0.4042834076599228	No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1599	0.39904269681988674	TruSeq Adapter, Index 20 (95% over 21bp)
AAAAGGTGGGAGAGTTTATTTGTAACCTTAATTATATTAAAAGTTATTCTG	1044	0.2605381960475058	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	1013	0.25280190861697643	No Hit
ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	967	0.24132225630070697	TruSeq Adapter, Index 27 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	833	0.20788152998809611	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	696	0.1736921306983372	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.16920009283544918	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.15647265222393308	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	622	0.15522486392868642	TruSeq Adapter, Index 27 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.13675759715903563	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC	518	0.12927086738755555	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	493	0.12303192591132218	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	483	0.12053634932082884	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGT	468	0.11679298443508881	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	443	0.11055404295885543	No Hit
AATGTAGGGATTTTTGGATACTAATATTTGACATGGTTAATTCTTATTAAT	441	0.11005492764075675	No Hit
AGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	423	0.10556288977786872	TruSeq Adapter, Index 27 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	420	0.10481421680072073	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	30	2.161949E-6	45.000004	2
TTCGGGA	30	2.161949E-6	45.000004	4
TCCGGAT	35	1.2092823E-7	45.0	27
TCACGAC	35	1.2092823E-7	45.0	25
GTCATAG	20	7.027781E-4	45.0	1
CTCCGGA	35	1.2092823E-7	45.0	26
CGAAAGG	20	7.027781E-4	45.0	2
GGATCGT	35	1.2092823E-7	45.0	8
TCGGGAT	45	3.8380676E-10	45.0	5
GATCATC	20	7.027781E-4	45.0	7
GATTTCC	50	2.1827873E-11	45.0	9
ACGGGTC	20	7.027781E-4	45.0	5
ATCGTGC	20	7.027781E-4	45.0	10
CGTTATT	210	0.0	45.0	1
CGTTAGG	55	1.8189894E-12	45.0	2
CTAACGG	25	3.8862418E-5	45.0	2
TACGCGG	20	7.027781E-4	45.0	2
AGGTTTA	35	1.2092823E-7	45.0	6
GTGCTAG	40	6.7957444E-9	45.0	1
CAGATAT	25	3.8862418E-5	45.0	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content