##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400709
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.527767032934126	31.0	31.0	34.0	30.0	34.0
2	31.643207414857166	31.0	31.0	34.0	30.0	34.0
3	31.650025330102395	31.0	31.0	34.0	30.0	34.0
4	35.47746369559955	37.0	35.0	37.0	33.0	37.0
5	32.88766161977894	37.0	35.0	37.0	28.0	37.0
6	33.93590361084977	36.0	35.0	37.0	26.0	37.0
7	35.261893294136144	37.0	35.0	37.0	32.0	37.0
8	35.24485100159966	37.0	35.0	37.0	33.0	37.0
9	37.32589984252912	39.0	37.0	39.0	34.0	39.0
10	36.73178541036014	39.0	37.0	39.0	32.0	39.0
11	36.55161226725629	39.0	35.0	39.0	32.0	39.0
12	36.95880302164414	39.0	37.0	39.0	33.0	39.0
13	36.9288860494773	39.0	37.0	39.0	33.0	39.0
14	37.85374423833755	39.0	37.0	41.0	33.0	41.0
15	37.977277775143556	39.0	37.0	41.0	33.0	41.0
16	37.97125844440729	40.0	37.0	41.0	33.0	41.0
17	37.94691908592021	39.0	37.0	41.0	33.0	41.0
18	37.85697601002224	39.0	37.0	40.0	33.0	41.0
19	37.62277612931079	39.0	36.0	40.0	33.0	41.0
20	37.45384555874712	39.0	35.0	40.0	33.0	41.0
21	37.37518997576795	39.0	35.0	40.0	33.0	41.0
22	37.32394081490558	39.0	35.0	40.0	33.0	41.0
23	37.05972913011687	39.0	35.0	40.0	32.0	41.0
24	36.75986064700319	38.0	35.0	40.0	32.0	41.0
25	36.58533249814703	38.0	35.0	40.0	31.0	41.0
26	36.89728206753529	38.0	35.0	40.0	32.0	41.0
27	36.747667259782034	39.0	35.0	40.0	32.0	41.0
28	36.842057453164266	39.0	35.0	40.0	32.0	41.0
29	36.87973566852754	39.0	35.0	40.0	32.0	41.0
30	36.639918244910895	39.0	35.0	40.0	31.0	41.0
31	36.49285142085653	38.0	35.0	40.0	31.0	41.0
32	36.18129615256957	38.0	35.0	40.0	30.0	41.0
33	36.0650746551737	38.0	35.0	40.0	30.0	41.0
34	35.966080122982014	39.0	35.0	40.0	30.0	41.0
35	35.72282629040026	39.0	35.0	40.0	27.0	41.0
36	35.633499621920144	39.0	35.0	40.0	27.0	41.0
37	35.5357279222578	38.0	35.0	40.0	27.0	41.0
38	35.331340199496395	38.0	35.0	40.0	25.0	41.0
39	35.287705042811616	38.0	35.0	40.0	25.0	41.0
40	35.24614870142672	38.0	35.0	40.0	24.0	41.0
41	35.13764851800184	38.0	35.0	40.0	24.0	41.0
42	35.225565185708334	38.0	35.0	40.0	24.0	41.0
43	35.09236877634393	38.0	35.0	40.0	23.0	41.0
44	34.93985660416911	38.0	35.0	40.0	23.0	41.0
45	34.893533711496374	38.0	35.0	40.0	23.0	41.0
46	34.76094871839664	38.0	34.0	40.0	23.0	41.0
47	34.651812162941184	38.0	34.0	40.0	22.0	41.0
48	34.59850415139166	38.0	34.0	40.0	22.0	41.0
49	34.602125233024466	38.0	34.0	40.0	22.0	41.0
50	34.50584339258664	38.0	34.0	40.0	21.0	41.0
51	33.593819954131305	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	13.0
11	6.0
12	14.0
13	22.0
14	21.0
15	42.0
16	45.0
17	108.0
18	208.0
19	340.0
20	588.0
21	967.0
22	1568.0
23	2328.0
24	3431.0
25	4734.0
26	6304.0
27	6899.0
28	7082.0
29	7159.0
30	7908.0
31	9419.0
32	12170.0
33	16186.0
34	24017.0
35	36094.0
36	46919.0
37	59029.0
38	86699.0
39	60369.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.71823442947376	24.89362604783022	23.91810515860637	17.470034364089653
2	35.62360715631543	24.74713570196826	24.783820677848514	14.845436463867795
3	25.97221425024145	24.74414101005967	35.8409718773474	13.442672862351483
4	23.641844830038757	26.37225517769753	34.83550406903763	15.150395923226082
5	17.93520984055761	33.88044690785583	32.74670646279469	15.437636788791867
6	21.367376325463116	33.37184839871328	32.77690293953967	12.483872336283937
7	65.21790126999892	8.028269891617109	21.12655318448051	5.627275653903456
8	63.254631166257816	7.741029026051324	20.82583620532606	8.17850360236481
9	58.26048329336252	10.00950814680978	23.609402334362343	8.120606225465362
10	27.044563511176438	31.236383510228123	29.156569979710962	12.562482998884478
11	16.921506629499213	26.37300385067468	36.94351761502737	19.761971904798745
12	17.611034441452524	22.40653441774455	42.314747110746204	17.667684030056723
13	18.843350161838142	25.0892792525249	41.83509729005338	14.232273295583578
14	15.400452697593517	28.1293906550639	38.06078725459124	18.409369392751348
15	12.110284520687083	27.753307262876554	42.40883034820781	17.727577868228565
16	12.687760943727245	29.04376991782066	37.384236440908495	20.884232697543602
17	12.45267762890277	28.938456585701843	36.384508458756855	22.224357326638533
18	12.414744864727272	28.63224933804831	41.080684486747245	17.87232131047718
19	12.51307058239271	29.34049397443032	38.89555762411126	19.250877819065707
20	13.07931691077565	31.937390974497703	38.13515543698794	16.848136677738708
21	13.832731483445592	30.21669091535254	39.35798796632968	16.59258963487219
22	12.255527078253795	29.10116817940201	34.83874831860527	23.804556423738923
23	11.449954954842543	28.48925279941304	36.3665403073053	23.694251938439116
24	14.80925060330564	27.89380822492133	35.057360827932484	22.239580343840544
25	11.395800942828837	31.183477286509664	32.999258813752625	24.42146295690888
26	11.840263133595702	30.405356505593833	34.77635890384294	22.978021456967525
27	16.724855194168338	28.31031995787467	35.26723881919298	19.697586028764018
28	13.162918726557177	27.465068166674566	35.21508126845167	24.156931838316584
29	14.643793875355932	26.73685891756861	34.35485601770861	24.264491189366847
30	20.32023238809211	28.448574900988	31.99528835139715	19.235904359522745
31	17.280869658530253	32.92214549710638	29.084447816245707	20.712537028117662
32	15.39820667866207	34.352360441118115	27.351020316489024	22.898412563730787
33	17.931716033330922	28.221976546571202	30.587284039040803	23.259023381057077
34	15.283659713158427	26.93301123758139	32.597221425024145	25.186107624236044
35	15.270682714887862	29.245662063991574	28.25691461883811	27.226740602282458
36	21.263061223980493	26.742099628408646	29.350226723133243	22.644612424477614
37	20.778420250106684	29.39265152517163	31.51688631899957	18.312041905722108
38	16.943717261154603	33.742691080060595	28.393422658338096	20.92016900044671
39	17.022327923755142	27.265921154753197	34.56099064408336	21.150760277408292
40	16.962434085583304	26.231005542675607	36.32486417824406	20.481696193497026
41	16.218253146298185	29.584311807321523	27.991135711950566	26.206299334429723
42	19.66164972586091	26.045833759661	28.58633072878323	25.706185785694856
43	22.63737525236518	24.59016393442623	29.299316960687182	23.473143852521407
44	19.779940056250297	24.45839749044818	29.60477553536357	26.156886917937953
45	20.93888582487541	22.772635503569923	31.12707725556451	25.16140141599016
46	21.829806667681535	25.024643818831123	33.387071415915294	19.758478097572056
47	15.622309456488374	26.645021699038455	36.263472994117926	21.469195850355245
48	16.707386158034883	25.154413801536773	33.572742314247996	24.565457726180345
49	20.10037209046964	21.52759234257279	35.74364439031817	22.628391176639408
50	18.34074103651278	20.58950510220634	34.38330559084024	26.686448270440643
51	17.225467858221304	20.173742042230145	30.67862214225286	31.922167957295695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5208.0
1	5509.0
2	5810.0
3	10397.0
4	14984.0
5	9590.0
6	4196.0
7	4324.5
8	4453.0
9	4853.5
10	5254.0
11	5901.0
12	6548.0
13	6432.0
14	6316.0
15	5953.5
16	5591.0
17	4986.0
18	4381.0
19	3936.5
20	3492.0
21	3412.5
22	3333.0
23	3376.5
24	3420.0
25	3897.0
26	4012.5
27	3651.0
28	3769.0
29	3887.0
30	4283.5
31	4680.0
32	5055.0
33	5430.0
34	5990.0
35	6550.0
36	7032.5
37	7515.0
38	8467.5
39	9420.0
40	10895.0
41	12370.0
42	16333.0
43	20296.0
44	24028.0
45	27760.0
46	36199.5
47	44639.0
48	52669.0
49	60699.0
50	57132.0
51	53565.0
52	40932.0
53	28299.0
54	21074.0
55	13849.0
56	10327.5
57	6806.0
58	5408.5
59	4011.0
60	3589.0
61	3167.0
62	2801.5
63	2436.0
64	2007.5
65	1579.0
66	1290.0
67	1001.0
68	850.0
69	699.0
70	538.0
71	377.0
72	323.0
73	269.0
74	236.0
75	156.0
76	109.0
77	73.5
78	38.0
79	29.5
80	21.0
81	15.0
82	9.0
83	9.0
84	9.0
85	5.0
86	1.0
87	1.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.03228533500282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20235257626759	29.62627532767888
2	11.465640063759603	9.409228292892479
3	4.982550244112159	6.133362699371941
4	2.719534826924792	4.463549159874223
5	1.8699426574123725	3.836401033951895
6	1.306885214512905	3.2174692213193925
7	0.9994936848897561	2.8708057048250915
8	0.7961562500867785	2.613448833984525
9	0.6129932518401717	2.263726261614345
>10	2.8701812309891133	18.861656296346876
>50	0.0997587981313217	2.7667888637120153
>100	0.0634268901699144	4.930695697395093
>500	0.00492636040154675	1.3869318788050458
>1k	0.005542155451740093	5.25336139410379
>5k	6.157950501933438E-4	2.3662993341244767
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9365	2.337107476997023	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4278	1.0676076654130553	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	3175	0.7923455674816388	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	3046	0.7601526294642746	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	2855	0.7124871165858516	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	2161	0.539294101205613	TruSeq Adapter, Index 27 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1620	0.4042834076599228	No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1599	0.39904269681988674	TruSeq Adapter, Index 20 (95% over 21bp)
AAAAGGTGGGAGAGTTTATTTGTAACCTTAATTATATTAAAAGTTATTCTG	1044	0.2605381960475058	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	1013	0.25280190861697643	No Hit
ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	967	0.24132225630070697	TruSeq Adapter, Index 27 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	833	0.20788152998809611	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	696	0.1736921306983372	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.16920009283544918	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.15647265222393308	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	622	0.15522486392868642	TruSeq Adapter, Index 27 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.13675759715903563	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC	518	0.12927086738755555	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	493	0.12303192591132218	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	483	0.12053634932082884	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGT	468	0.11679298443508881	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	443	0.11055404295885543	No Hit
AATGTAGGGATTTTTGGATACTAATATTTGACATGGTTAATTCTTATTAAT	441	0.11005492764075675	No Hit
AGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	423	0.10556288977786872	TruSeq Adapter, Index 27 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	420	0.10481421680072073	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5807206726078027	0.0
2	0.0	0.0	0.0	2.87490423224834	0.0
3	0.0	0.0	0.0	3.84318794935976	0.0
4	0.0	0.0	0.0	5.1047019158541485	0.0
5	0.0	0.0	0.0	8.507171039332782	0.0
6	0.0	0.0	0.0	10.22013481104742	0.0
7	0.0	0.0	0.0	12.177165973312304	0.0
8	0.0	0.0	0.0	15.154388845770871	0.0
9	0.0	0.0	0.0	16.247950507725058	0.0
10	0.0	0.0	0.0	19.440042524625103	0.0
11	0.0	0.0	0.0	24.75587022003499	0.0
12	0.0	0.0	0.0	28.389679293452357	0.0
13	0.0	0.0	0.0	29.696612753893724	0.0
14	0.0	0.0	0.0	30.22018472257923	0.0
15	0.0	0.0	0.0	31.205188802846955	0.0
16	0.0	0.0	0.0	33.386073185279095	0.0
17	0.0	0.0	0.0	36.218552615489045	0.0
18	0.0	0.0	0.0	39.04678956549516	0.0
19	0.0	0.0	0.0	40.81665248347304	0.0
20	0.0	0.0	0.0	42.4457648817471	0.0
21	0.0	0.0	0.0	44.34864203199828	0.0
22	0.0	0.0	0.0	46.242285548864636	0.0
23	0.0	0.0	0.0	48.02886882999883	0.0
24	0.0	0.0	0.0	49.22874205470803	0.0
25	0.0	0.0	0.0	50.116169090287464	0.0
26	0.0	0.0	0.0	50.86633941338977	0.0
27	0.0	0.0	0.0	51.5124441926685	0.0
28	0.0	0.0	0.0	52.214449887574276	0.0
29	0.0	0.0	0.0	52.86903962726068	0.0
30	0.0	0.0	0.0	53.53386123096811	0.0
31	2.4955765904933504E-4	0.0	0.0	54.089626137670976	0.0
32	2.4955765904933504E-4	0.0	0.0	54.578260034089574	0.0
33	2.4955765904933504E-4	0.0	0.0	54.99327442108862	0.0
34	2.4955765904933504E-4	0.0	0.0	55.43898440015073	0.0
35	2.4955765904933504E-4	0.0	0.0	55.91813510552546	0.0
36	2.4955765904933504E-4	0.0	0.0	56.27026096244407	0.0
37	2.4955765904933504E-4	0.0	0.0	56.65557798801624	0.0
38	2.4955765904933504E-4	0.0	0.0	57.00670561429865	0.0
39	2.4955765904933504E-4	0.0	0.0	57.33537305126663	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	30	2.161949E-6	45.000004	2
TTCGGGA	30	2.161949E-6	45.000004	4
TCCGGAT	35	1.2092823E-7	45.0	27
TCACGAC	35	1.2092823E-7	45.0	25
GTCATAG	20	7.027781E-4	45.0	1
CTCCGGA	35	1.2092823E-7	45.0	26
CGAAAGG	20	7.027781E-4	45.0	2
GGATCGT	35	1.2092823E-7	45.0	8
TCGGGAT	45	3.8380676E-10	45.0	5
GATCATC	20	7.027781E-4	45.0	7
GATTTCC	50	2.1827873E-11	45.0	9
ACGGGTC	20	7.027781E-4	45.0	5
ATCGTGC	20	7.027781E-4	45.0	10
CGTTATT	210	0.0	45.0	1
CGTTAGG	55	1.8189894E-12	45.0	2
CTAACGG	25	3.8862418E-5	45.0	2
TACGCGG	20	7.027781E-4	45.0	2
AGGTTTA	35	1.2092823E-7	45.0	6
GTGCTAG	40	6.7957444E-9	45.0	1
CAGATAT	25	3.8862418E-5	45.0	18
>>END_MODULE