FastQCFastQC Report
Sat 14 Jan 2017
SRR2935196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671613
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2052377.682138094102702No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC94000.3518473671149227No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88590.33159742822032984No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG70810.2650458730362519No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC64880.24284954445123602No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC52140.1951629970358731No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT51480.1926925793518747No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT45350.16974763934746537No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGT40490.15155638185620446No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC40140.15024631187226595No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC38500.1441076982332396No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG38210.1430222116751191No Hit
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG35630.13336512436494358No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC34470.12902317813246156No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC33770.12640303816458448No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA31010.11607220057695482No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT28110.10521733499574977No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT27230.10192344475041858Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT740900.044.3683361
CGTTATT54800.044.219891
CGGTCTA5150.041.0679631
TCACGAC5250.040.71428325
GTTATTT61250.040.224492
GTTTTTT840950.039.3679772
CTCACGA5600.038.16964324
ACCACCG12550.037.47012314
TACGGCT6300.037.1428577
CGACCAC17500.037.0285712
CGTAAGG3100.037.016132
TACGGGA16350.036.7431224
AGTACGG3250.036.6923072
ATCGCCA1300.036.34615324
CCGATGA9850.036.09137318
GTCGATA250.002107606736.00000411
CCGTACC250.002107606736.00000420
ACGACGT250.002107606736.00000435
TCTCACG6000.035.62523
TATGGGA23150.035.4751634
Produced by FastQC (version 0.11.5)