##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671613
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.141333718618675	33.0	31.0	34.0	30.0	34.0
2	32.28690046050831	34.0	31.0	34.0	30.0	34.0
3	32.18183209918502	33.0	31.0	34.0	30.0	34.0
4	35.88920476131835	37.0	35.0	37.0	35.0	37.0
5	33.36832729890145	37.0	35.0	37.0	30.0	37.0
6	34.42986540340985	37.0	35.0	37.0	29.0	37.0
7	35.7057620246645	37.0	35.0	37.0	32.0	37.0
8	35.70087284348444	37.0	35.0	37.0	35.0	37.0
9	37.828928441357334	39.0	38.0	39.0	35.0	39.0
10	37.43451053726719	39.0	37.0	39.0	34.0	39.0
11	37.332710239095256	39.0	37.0	39.0	34.0	39.0
12	37.49778280012861	39.0	37.0	39.0	35.0	39.0
13	37.44993904431517	39.0	37.0	39.0	35.0	39.0
14	38.59115186218962	40.0	38.0	41.0	34.0	41.0
15	38.697947270057455	40.0	38.0	41.0	34.0	41.0
16	38.70397733504067	40.0	38.0	41.0	34.0	41.0
17	38.614907922666944	40.0	38.0	41.0	34.0	41.0
18	38.20774940083014	39.0	38.0	40.0	34.0	41.0
19	37.67505323562956	38.0	37.0	40.0	34.0	41.0
20	37.10241378523012	38.0	35.0	40.0	34.0	41.0
21	37.0049370923109	38.0	35.0	40.0	33.0	41.0
22	36.982900592263924	38.0	35.0	40.0	33.0	41.0
23	36.7814975447417	38.0	35.0	40.0	33.0	41.0
24	36.51581273185899	37.0	35.0	40.0	33.0	41.0
25	36.33098768421923	37.0	35.0	40.0	32.0	41.0
26	36.55447252277931	38.0	35.0	40.0	33.0	41.0
27	36.45590922038484	38.0	35.0	40.0	33.0	41.0
28	36.42260911292167	38.0	35.0	40.0	33.0	41.0
29	36.36764755973264	38.0	35.0	40.0	33.0	41.0
30	35.95801113409764	37.0	35.0	40.0	31.0	41.0
31	35.5502028175488	37.0	35.0	40.0	30.0	41.0
32	34.85089831498799	37.0	35.0	40.0	22.0	41.0
33	34.102875304170176	38.0	34.0	40.0	18.0	41.0
34	33.41688710153753	38.0	33.0	40.0	12.0	41.0
35	32.938391900323886	38.0	33.0	40.0	10.0	41.0
36	32.58878175843582	37.0	33.0	40.0	9.0	41.0
37	32.48224724164765	37.0	32.0	40.0	8.0	41.0
38	32.37880860738438	37.0	32.0	40.0	8.0	41.0
39	32.221971894881484	37.0	31.0	40.0	8.0	41.0
40	32.10660713209585	37.0	31.0	40.0	8.0	41.0
41	31.905059228263973	37.0	30.0	40.0	8.0	41.0
42	31.845057274388168	37.0	30.0	40.0	7.0	41.0
43	31.71717797450454	36.0	30.0	40.0	7.0	41.0
44	31.633643794965813	36.0	30.0	40.0	7.0	41.0
45	31.642660819512407	36.0	29.0	40.0	7.0	41.0
46	31.581570758938515	36.0	29.0	40.0	7.0	41.0
47	31.51274267642806	36.0	29.0	40.0	7.0	41.0
48	31.458781268095343	36.0	29.0	40.0	7.0	41.0
49	31.43671370067446	35.0	29.0	40.0	7.0	41.0
50	31.34749456601686	35.0	28.0	40.0	7.0	41.0
51	30.655744301289147	35.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	21.0
9	42.0
10	54.0
11	60.0
12	69.0
13	112.0
14	172.0
15	254.0
16	483.0
17	895.0
18	1782.0
19	3299.0
20	5823.0
21	9598.0
22	15278.0
23	24343.0
24	41430.0
25	71367.0
26	103006.0
27	112103.0
28	99377.0
29	83393.0
30	71438.0
31	69751.0
32	74741.0
33	92406.0
34	134208.0
35	189645.0
36	238764.0
37	258659.0
38	429299.0
39	539562.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.82366832321897	13.889399400287392	17.315868727993163	38.971063548500474
2	51.68098822696251	18.27079745457145	19.12174405499599	10.926470263470046
3	21.663803851830334	18.6245912113768	49.100150358603585	10.61145457818928
4	18.913442927549763	18.33727414861359	50.334498297470475	12.414784626366169
5	16.89234181747132	26.88417072382864	44.549865568104366	11.673621890595681
6	18.150308446620077	24.856594124972442	46.72577951971337	10.26731790869411
7	56.27929644001583	4.321059973880947	36.332694892561165	3.066948693542066
8	57.29407664957462	5.022471443281643	34.8219596176542	2.8614922894895334
9	52.139512721341006	7.074939371832673	35.66287482505887	5.122673081767457
10	20.888841310474234	30.58538044245181	39.92741463677561	8.598363610298348
11	19.881322631683556	19.172649631514744	48.12089176089501	12.82513597590669
12	18.831694560551995	17.045732297305037	49.626087311298456	14.496485830844513
13	18.14746372322638	17.36729833250549	51.440384516769456	13.044853427498667
14	13.747724689167182	20.237100208750295	51.48900682845906	14.526168273623464
15	12.572966219284007	20.714789155465258	52.53096163254184	14.1812829927089
16	16.03454542255933	21.344670803742908	49.039250819635924	13.581532954061833
17	15.743335580415277	21.317496209218927	48.940770987414716	13.998397222951079
18	15.292783797653328	20.83400552400366	50.01854684791547	13.854663830427535
19	14.807496445031523	21.816071414534967	48.889902841466935	14.48652929896658
20	15.249663779896263	23.010069197896552	49.15274031081597	12.58752671139121
21	15.641037829955163	21.544961789001626	50.53171997590968	12.282280405133527
22	15.228290923872581	19.120621137866898	50.69925921156994	14.95182872669058
23	13.885356898622666	20.459175786313363	51.04384504791675	14.611622267147226
24	14.081867396213449	21.183345042863618	49.546771931413716	15.188015629509213
25	14.558545717512231	22.942357295012414	47.84858435709064	14.650512630384716
26	14.112335880982762	21.94344764754476	48.37905040887284	15.565166062599637
27	14.431206915073403	21.380267276735065	49.02162102070921	15.166904787482318
28	12.833782437800684	21.7214843616946	49.524650463970644	15.920082736534072
29	14.536311958356244	20.96512481411043	49.50417594165023	14.994387285883098
30	16.14432928721338	21.384758945251427	48.298836695284834	14.17207507225036
31	15.82976276878425	21.94097722986076	47.24722480389188	14.982035197463105
32	16.877332158512477	23.12883639958332	45.635838723647474	14.357992718256723
33	17.87182499860571	22.47076953136551	43.86024472855911	15.797160741469666
34	17.381634241186877	23.68408148934745	43.50371854007298	15.430565729392692
35	18.46992809213011	24.640844313903248	41.02210911535465	15.867118478611985
36	18.984823026388927	24.91775567793689	40.95641846330288	15.141002832371306
37	18.426022032382683	26.207762875835684	40.35416057640085	15.012054515380783
38	18.816572609880247	26.02727266261992	39.00841925832821	16.14773546917162
39	19.755817927222243	25.32795730519353	38.50261995281502	16.413604814769204
40	20.62409488200574	24.458145697000276	38.90342650675828	16.01433291423571
41	18.915015011530485	24.965591947636128	38.91525456718469	17.20413847364869
42	18.374292983302595	25.14638160541965	39.86314634642068	16.61617906485707
43	18.38649534943871	24.91423720426574	39.63362208523465	17.065645361060902
44	18.682009707244276	24.823018902812645	38.71941781987137	17.775553570071715
45	18.324547754483902	24.331892381119573	38.491540503808	18.85201936058853
46	18.8985829908748	24.469898896284754	39.06871990816035	17.562798204680092
47	17.02499576098784	24.907686854346046	41.04505405535907	17.02226332930705
48	17.470868722378576	24.791614653769088	40.089039842222654	17.648476781629675
49	17.849516378307786	23.694300035222167	41.02877175698726	17.42741182948279
50	17.32447775931619	23.66764946869176	40.483370907388164	18.52450186460389
51	17.15499213396551	23.414394225510957	39.644738964812646	19.78587467571089
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11268.0
1	12043.5
2	12819.0
3	161033.0
4	309247.0
5	192525.5
6	75804.0
7	71346.5
8	66889.0
9	66622.0
10	66355.0
11	63816.5
12	61278.0
13	58843.0
14	56408.0
15	53418.5
16	50429.0
17	46884.0
18	43339.0
19	40311.0
20	37283.0
21	35837.5
22	34392.0
23	33576.5
24	32761.0
25	33225.0
26	34773.5
27	35858.0
28	39758.0
29	43658.0
30	47060.5
31	50463.0
32	54693.0
33	58923.0
34	63193.5
35	67464.0
36	71633.5
37	75803.0
38	80402.5
39	85002.0
40	88881.5
41	92761.0
42	99081.0
43	105401.0
44	114623.5
45	123846.0
46	151368.0
47	178890.0
48	175081.0
49	171272.0
50	166539.0
51	161806.0
52	139840.5
53	117875.0
54	104480.0
55	91085.0
56	84538.0
57	77991.0
58	71810.0
59	65629.0
60	58104.0
61	50579.0
62	44603.5
63	38628.0
64	34616.0
65	30604.0
66	25346.5
67	20089.0
68	16873.5
69	13658.0
70	11425.5
71	9193.0
72	7464.5
73	5736.0
74	4726.5
75	2807.5
76	1898.0
77	1410.0
78	922.0
79	658.5
80	395.0
81	306.5
82	218.0
83	197.0
84	176.0
85	116.0
86	56.0
87	38.5
88	21.0
89	20.0
90	19.0
91	15.0
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2671613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.207650357440684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95012859501286	23.546902299156873
2	7.959800832580151	4.80893760930893
3	3.112342860175614	2.820496947379856
4	1.7025437014833211	2.0571937941068414
5	1.2067872465818137	1.822710360028099
6	0.8749089561303692	1.5857366304827765
7	0.6899316713322939	1.4588850288691433
8	0.5537121721357232	1.3381074955627952
9	0.49008653430842475	1.3323926405950879
>10	4.930149011082453	33.0837318375044
>50	0.4393933223632669	8.6173200035739
>100	0.08262333516709452	4.585523399367374
>500	0.002702152450497334	0.5346870500329007
>1k	0.0039888917126389214	2.790043359360397
>5k	7.720435572849525E-4	1.6398987089915735
>10k+	1.2867392621415875E-4	7.977432835679155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205237	7.682138094102702	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	9400	0.3518473671149227	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8859	0.33159742822032984	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	7081	0.2650458730362519	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC	6488	0.24284954445123602	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	5214	0.1951629970358731	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT	5148	0.1926925793518747	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	4535	0.16974763934746537	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGT	4049	0.15155638185620446	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	4014	0.15024631187226595	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	3850	0.1441076982332396	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	3821	0.1430222116751191	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	3563	0.13336512436494358	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	3447	0.12902317813246156	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	3377	0.12640303816458448	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	3101	0.11607220057695482	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	2811	0.10521733499574977	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	2723	0.10192344475041858	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.486114193934526E-5	0.0	0.0	0.1092598366604744	0.0
2	7.486114193934526E-5	0.0	0.0	0.4644010940207283	0.0
3	7.486114193934526E-5	0.0	0.0	0.7193032823241989	0.0
4	7.486114193934526E-5	0.0	0.0	1.0910637131949874	0.0
5	7.486114193934526E-5	0.0	0.0	2.13466546240043	0.0
6	7.486114193934526E-5	0.0	0.0	2.9307388457834276	0.0
7	7.486114193934526E-5	0.0	0.0	3.622081491593281	0.0
8	7.486114193934526E-5	0.0	0.0	4.409620704795193	0.0
9	7.486114193934526E-5	0.0	0.0	4.87529443822889	0.0
10	7.486114193934526E-5	0.0	0.0	5.959845232075154	0.0
11	7.486114193934526E-5	0.0	0.0	6.999479340757812	0.0
12	7.486114193934526E-5	0.0	0.0	7.93269833617369	0.0
13	7.486114193934526E-5	0.0	0.0	8.267814238065169	0.0
14	7.486114193934526E-5	0.0	0.0	8.45979563656862	0.0
15	7.486114193934526E-5	0.0	0.0	8.72390574533063	0.0
16	7.486114193934526E-5	0.0	0.0	9.114306600544316	0.0
17	7.486114193934526E-5	0.0	0.0	9.59162124154958	0.0
18	7.486114193934526E-5	0.0	0.0	10.06493081146109	0.0
19	7.486114193934526E-5	0.0	0.0	10.47427153558543	0.0
20	7.486114193934526E-5	0.0	0.0	10.85958183314724	0.0
21	7.486114193934526E-5	0.0	0.0	11.281162353978663	0.0
22	7.486114193934526E-5	0.0	0.0	11.744365669728362	0.0
23	7.486114193934526E-5	0.0	0.0	12.117660754008908	0.0
24	7.486114193934526E-5	0.0	0.0	12.388807810113216	0.0
25	7.486114193934526E-5	0.0	0.0	12.624770129506032	0.0
26	7.486114193934526E-5	0.0	0.0	12.846359109646494	0.0
27	7.486114193934526E-5	0.0	0.0	13.074273856280831	0.0
28	1.1229171290901788E-4	0.0	0.0	13.277297273220336	0.0
29	1.1229171290901788E-4	0.0	0.0	13.49402027913474	0.0
30	1.1229171290901788E-4	0.0	0.0	13.721148983778713	0.0
31	1.1229171290901788E-4	0.0	0.0	13.91698573109204	0.0
32	1.1229171290901788E-4	0.0	0.0	14.110501783005247	0.0
33	1.1229171290901788E-4	0.0	0.0	14.285826577427194	0.0
34	1.1229171290901788E-4	0.0	0.0	14.466541374068774	0.0
35	1.4972228387869052E-4	0.0	0.0	14.651336102946049	0.0
36	1.8715285484836313E-4	0.0	0.0	14.82254353456133	0.0
37	1.8715285484836313E-4	0.0	0.0	15.005167290322364	0.0
38	1.8715285484836313E-4	0.0	0.0	15.226905992746705	0.0
39	2.2458342581803577E-4	0.0	0.0	15.582234402961806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	74090	0.0	44.368336	1
CGTTATT	5480	0.0	44.21989	1
CGGTCTA	515	0.0	41.06796	31
TCACGAC	525	0.0	40.714283	25
GTTATTT	6125	0.0	40.22449	2
GTTTTTT	84095	0.0	39.367977	2
CTCACGA	560	0.0	38.169643	24
ACCACCG	1255	0.0	37.470123	14
TACGGCT	630	0.0	37.142857	7
CGACCAC	1750	0.0	37.02857	12
CGTAAGG	310	0.0	37.01613	2
TACGGGA	1635	0.0	36.743122	4
AGTACGG	325	0.0	36.692307	2
ATCGCCA	130	0.0	36.346153	24
CCGATGA	985	0.0	36.091373	18
GTCGATA	25	0.0021076067	36.000004	11
CCGTACC	25	0.0021076067	36.000004	20
ACGACGT	25	0.0021076067	36.000004	35
TCTCACG	600	0.0	35.625	23
TATGGGA	2315	0.0	35.475163	4
>>END_MODULE