SRR2935195.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935195.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267841
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7129	2.661653742332205	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5907	2.205412912884883	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2773	1.035315728361229	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2394	0.8938138671823956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	2231	0.8329568662004696	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2118	0.7907676569307911	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1513	0.5648873772126	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	746	0.2785234523467281	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	648	0.24193458059072362	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	639	0.2385743780825191	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	632	0.2359608872428045	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	560	0.20907926717716854	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	507	0.18929140796218652	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	495	0.1848111379512472	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	424	0.15830287371985619	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.1515824687034472	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	393	0.14672884285826293	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	371	0.13851501450487416	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	369	0.13776830283638428	No Hit
GATTTAGGGAAGTATACTTTTAAATAATAAATTTTCAGAATTAATGTTTTC	352	0.13142125365422022	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT	342	0.1276876953117708	No Hit
GATAAGGGGATAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	325	0.12134064612960675	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.11611366445017754	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	306	0.1142468852789528	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	304	0.11350017361046291	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	300	0.11200675027348314	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	293	0.10939325943376853	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	45	3.8198777E-10	45.000004	2
GTTATGG	55	1.8189894E-12	45.000004	2
ACAACGA	20	7.0235884E-4	45.0	13
GTTTGCG	20	7.0235884E-4	45.0	1
GATACTC	20	7.0235884E-4	45.0	9
ACTATGC	20	7.0235884E-4	45.0	33
GGTACAC	25	3.8827682E-5	45.0	8
ATTAGTG	25	3.8827682E-5	45.0	14
ATTAGAC	20	7.0235884E-4	45.0	18
GTACACT	25	3.8827682E-5	45.0	9
CACGGGA	70	0.0	45.0	4
CGACGGT	20	7.0235884E-4	45.0	28
CAACGAG	20	7.0235884E-4	45.0	14
ACGTTGG	20	7.0235884E-4	45.0	2
ACTTGCG	20	7.0235884E-4	45.0	1
CGCATTA	20	7.0235884E-4	45.0	15
TAGGTGC	20	7.0235884E-4	45.0	11
ATGGACC	25	3.8827682E-5	45.0	10
GGACTAT	20	7.0235884E-4	45.0	8
AAGGTCT	20	7.0235884E-4	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content