##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935195.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267841
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.689812239350957	31.0	31.0	34.0	30.0	34.0
2	31.794919373807595	33.0	31.0	34.0	30.0	34.0
3	31.793313943720342	33.0	31.0	34.0	30.0	34.0
4	35.60998876198939	37.0	35.0	37.0	33.0	37.0
5	33.05027609663942	37.0	35.0	37.0	28.0	37.0
6	34.092760257018156	37.0	35.0	37.0	28.0	37.0
7	35.35580811003543	37.0	35.0	37.0	32.0	37.0
8	35.29595170268928	37.0	35.0	37.0	33.0	37.0
9	37.396694307443596	39.0	37.0	39.0	34.0	39.0
10	36.9273860237977	39.0	37.0	39.0	33.0	39.0
11	36.858330128695755	39.0	37.0	39.0	33.0	39.0
12	37.169835835439685	39.0	37.0	39.0	34.0	39.0
13	37.09593378160924	39.0	37.0	39.0	33.0	39.0
14	38.06413879876494	40.0	37.0	41.0	33.0	41.0
15	38.22147094731576	40.0	38.0	41.0	33.0	41.0
16	38.19041147546492	40.0	37.0	41.0	33.0	41.0
17	38.103792921920096	40.0	37.0	41.0	33.0	41.0
18	37.93747036488066	39.0	37.0	40.0	34.0	41.0
19	37.62812638841701	39.0	36.0	40.0	33.0	41.0
20	37.41979009934999	39.0	35.0	40.0	33.0	41.0
21	37.303713770483235	39.0	35.0	40.0	33.0	41.0
22	37.26025515137712	39.0	35.0	40.0	33.0	41.0
23	36.985651935289965	39.0	35.0	40.0	33.0	41.0
24	36.67361232970307	38.0	35.0	40.0	32.0	41.0
25	36.487330169764896	38.0	35.0	40.0	31.0	41.0
26	36.7680414873003	38.0	35.0	40.0	32.0	41.0
27	36.67381020829522	38.0	35.0	40.0	32.0	41.0
28	36.742884771188876	38.0	35.0	40.0	32.0	41.0
29	36.788669397142336	39.0	35.0	40.0	33.0	41.0
30	36.4381032030197	38.0	35.0	40.0	31.0	41.0
31	36.274599482528814	38.0	35.0	40.0	31.0	41.0
32	35.916021072203286	38.0	35.0	40.0	30.0	41.0
33	35.597324532091804	38.0	35.0	40.0	28.0	41.0
34	35.4744456599251	38.0	35.0	40.0	26.0	41.0
35	35.17112017950949	38.0	35.0	40.0	24.0	41.0
36	34.95124719516429	38.0	35.0	40.0	23.0	41.0
37	34.9642474453127	38.0	35.0	40.0	23.0	41.0
38	34.819400315859035	38.0	35.0	40.0	23.0	41.0
39	34.6309489585239	38.0	35.0	40.0	21.0	41.0
40	34.540708853386896	38.0	35.0	40.0	21.0	41.0
41	34.386415821326835	38.0	34.0	40.0	21.0	41.0
42	34.44591380707211	38.0	35.0	40.0	21.0	41.0
43	34.36927505497665	38.0	34.0	40.0	20.0	41.0
44	34.29395051541773	38.0	34.0	40.0	18.0	41.0
45	34.26948450759966	38.0	34.0	40.0	20.0	41.0
46	34.05445394842462	38.0	34.0	40.0	18.0	41.0
47	33.91870923420985	38.0	33.0	40.0	18.0	41.0
48	33.863097882699066	37.0	33.0	40.0	18.0	41.0
49	33.850541925993404	37.0	33.0	40.0	18.0	41.0
50	33.74354187745715	37.0	33.0	40.0	16.0	41.0
51	32.826016181241854	36.0	32.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	14.0
11	6.0
12	9.0
13	19.0
14	17.0
15	35.0
16	45.0
17	77.0
18	138.0
19	277.0
20	473.0
21	835.0
22	1254.0
23	1914.0
24	2646.0
25	3995.0
26	5056.0
27	5355.0
28	5155.0
29	4978.0
30	5507.0
31	6385.0
32	8082.0
33	10604.0
34	15789.0
35	26646.0
36	30908.0
37	37226.0
38	53805.0
39	40572.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.306853693049234	26.611310441642615	21.41270380561602	19.66913205969213
2	36.41675471641758	26.148349207178885	23.03568161707879	14.39921445932475
3	26.03932930357936	25.331819997685194	35.85858774422139	12.770262954514058
4	23.815995310650724	26.637445350039762	34.753081119022106	14.79347822028741
5	16.73828876086932	34.63808752207466	33.35710365478026	15.266520062275754
6	19.603048077030778	33.854413625994525	34.485384985868485	12.057153311106216
7	61.13253758759861	10.616746502589223	22.727289697992465	5.523426211819699
8	58.34020930328067	10.235550195825136	23.09168499221553	8.332555508678656
9	52.737631654601046	13.260852520711916	26.145362360504926	7.856153464182109
10	21.834595898312806	35.95939381946752	30.786175380169578	11.419834902050097
11	16.780851325973245	27.36287573597769	37.54727618251126	18.3089967555378
12	17.488360631867415	24.513424009020277	41.56943858483205	16.428776774280262
13	17.52569621529191	27.48085617959909	41.99469088003704	12.998756725071964
14	12.887123330632727	30.934771002199067	38.75508230629366	17.42302336087455
15	11.352630851886007	29.4406009535508	43.00760525834357	16.199162936219622
16	11.423941816226792	31.41527996087231	38.81220574893314	18.348572473967764
17	10.981888508480777	31.1095015326257	36.26703902688536	21.641570932008168
18	11.549762732367338	30.054024589215246	41.88193741809506	16.514275260322357
19	11.52810809398113	31.390265119977894	38.795404736392115	18.28622204964886
20	12.084034931171853	33.62890670211058	38.857755160711015	15.429303206006548
21	12.598892626595628	32.1545245126773	40.214530262357144	15.032052598369926
22	10.627947177616571	31.603077945497514	35.97134120616336	21.797633670722554
23	10.795957303026796	30.537520394562446	37.15151899821162	21.515003304199134
24	14.614267419849835	29.427906855186475	35.953793481953845	20.004032243009846
25	10.927751912515262	32.49539838934293	34.44431584410154	22.132533854040272
26	10.611892876744038	32.31618758890536	37.99231633693124	19.079603197419363
27	13.582311893996813	30.47367654690656	35.902643732662284	20.04136782643434
28	9.838299588188516	29.43052034602619	36.44886331816264	24.282316747622655
29	13.7824306211521	27.541713180581016	37.25157836178927	21.42427783647761
30	15.775777420185857	31.07403272837243	32.342322497302504	20.80786735413921
31	15.047733543408215	33.090154233295124	29.59293013392274	22.26918208937392
32	15.831034083654108	34.16952595009726	29.306566209056868	20.69287375719177
33	15.393087690084789	34.547362054353144	27.997954010028337	22.06159624553373
34	16.487766996090965	30.37473725083165	30.48114366359146	22.656352089485924
35	15.151899821162557	33.35486351977479	29.388331136756506	22.104905522306144
36	15.470745703607738	34.922211311935065	28.081959072733447	21.525083911723748
37	16.928700236334244	31.782288745935087	29.50892507121762	21.780085946513044
38	17.56527193372187	35.35978434967014	25.410598078710876	21.66434563789711
39	20.808240709973454	33.745393722395	28.283571223225717	17.162794344405825
40	21.226772600162036	30.990774377335807	30.755560201761494	17.026892820740663
41	16.406748780059814	34.816551610843746	27.11310068286782	21.66359892622862
42	16.839468191949702	32.29453295051915	30.122722062716313	20.743276794814836
43	18.869030506905215	29.507431647880644	30.23136861048159	21.392169234732545
44	17.573859117909507	28.230181338928695	28.697622843403366	25.49833669975844
45	20.16756209840913	26.041196082750588	28.521025533805506	25.27021628503478
46	21.37574157802577	28.706210027590995	30.339641802412626	19.57840659197061
47	15.061174353441034	30.460609092707987	34.053038929812836	20.42517762403814
48	15.325883639920699	29.01236181167185	32.18289955607992	23.478854992327538
49	19.324151268849803	26.019914800198624	34.74188044399476	19.914053486956814
50	18.117091856735897	25.01969452025642	32.87846147527824	23.984752147729438
51	16.23313831713591	25.111913411314923	28.69836955507185	29.95657871647731
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6793.0
1	6825.0
2	6857.0
3	9785.5
4	12714.0
5	8206.0
6	3698.0
7	3670.0
8	3642.0
9	4117.5
10	4593.0
11	4709.0
12	4825.0
13	4717.0
14	4609.0
15	4260.0
16	3911.0
17	3885.0
18	3859.0
19	3625.0
20	3391.0
21	3169.0
22	2947.0
23	3016.5
24	3086.0
25	3087.0
26	3027.5
27	2967.0
28	3317.0
29	3667.0
30	3616.0
31	3565.0
32	3947.0
33	4329.0
34	4525.5
35	4722.0
36	4841.0
37	4960.0
38	5208.0
39	5456.0
40	6452.5
41	7449.0
42	9912.5
43	12376.0
44	14239.0
45	16102.0
46	20773.5
47	25445.0
48	30799.5
49	36154.0
50	34741.5
51	33329.0
52	25023.0
53	16717.0
54	12722.5
55	8728.0
56	6463.5
57	4199.0
58	3493.5
59	2788.0
60	2396.0
61	2004.0
62	1989.0
63	1974.0
64	1537.5
65	1101.0
66	902.0
67	703.0
68	608.5
69	514.0
70	377.0
71	240.0
72	206.5
73	173.0
74	130.0
75	62.0
76	37.0
77	27.0
78	17.0
79	14.0
80	11.0
81	8.0
82	5.0
83	3.0
84	1.0
85	2.0
86	3.0
87	4.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	267841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.5243248095102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25316788357937	27.835045080630817
2	11.437585984317575	8.812505549931034
3	4.651949296523482	5.376396170899293
4	2.6614766167197796	4.101263586217162
5	1.7242498481051243	3.321278060057521
6	1.2397339585695903	2.865594821838885
7	0.9829955806293631	2.6508468724135055
8	0.8021802940141666	2.4722763361912112
9	0.6493364739020959	2.251372430813974
>10	3.3995261839963224	21.02320029349614
>50	0.10289485658203264	2.790162412855136
>100	0.08191629358957939	5.802383268774743
>500	0.00599387514070093	1.43626015514143
>1k	0.004994895950584108	4.244510517431626
>5k	0.0019979583802336434	5.016904443307524
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7129	2.661653742332205	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5907	2.205412912884883	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2773	1.035315728361229	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2394	0.8938138671823956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	2231	0.8329568662004696	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2118	0.7907676569307911	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1513	0.5648873772126	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	746	0.2785234523467281	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	648	0.24193458059072362	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	639	0.2385743780825191	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	632	0.2359608872428045	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	560	0.20907926717716854	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	507	0.18929140796218652	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	495	0.1848111379512472	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	424	0.15830287371985619	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.1515824687034472	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	393	0.14672884285826293	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	371	0.13851501450487416	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	369	0.13776830283638428	No Hit
GATTTAGGGAAGTATACTTTTAAATAATAAATTTTCAGAATTAATGTTTTC	352	0.13142125365422022	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT	342	0.1276876953117708	No Hit
GATAAGGGGATAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	325	0.12134064612960675	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.11611366445017754	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	306	0.1142468852789528	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	304	0.11350017361046291	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	300	0.11200675027348314	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	293	0.10939325943376853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.733558342449438E-4	0.0	0.0	0.598489402294645	0.0
2	3.733558342449438E-4	0.0	0.0	2.9566048513857104	0.0
3	3.733558342449438E-4	0.0	0.0	3.9131424987212564	0.0
4	3.733558342449438E-4	0.0	0.0	5.161271052602103	0.0
5	3.733558342449438E-4	0.0	0.0	8.640574071930734	0.0
6	3.733558342449438E-4	0.0	0.0	10.423721536284587	0.0
7	3.733558342449438E-4	0.0	0.0	12.188201208926191	0.0
8	3.733558342449438E-4	0.0	0.0	14.940580418979918	0.0
9	3.733558342449438E-4	0.0	0.0	15.88405061211689	0.0
10	3.733558342449438E-4	0.0	0.0	18.510982261864314	0.0
11	3.733558342449438E-4	0.0	0.0	23.052109273785568	0.0
12	3.733558342449438E-4	0.0	0.0	26.164776863885663	0.0
13	3.733558342449438E-4	0.0	0.0	27.332633913403846	0.0
14	3.733558342449438E-4	0.0	0.0	27.769460239470433	0.0
15	3.733558342449438E-4	0.0	0.0	28.69575606423214	0.0
16	3.733558342449438E-4	0.0	0.0	30.425140288454717	0.0
17	3.733558342449438E-4	0.0	0.0	32.60180480210274	0.0
18	3.733558342449438E-4	0.0	0.0	34.788549923275376	0.0
19	3.733558342449438E-4	0.0	0.0	36.09193514062447	0.0
20	3.733558342449438E-4	0.0	0.0	37.39382693463659	0.0
21	3.733558342449438E-4	0.0	0.0	38.99178990520495	0.0
22	3.733558342449438E-4	0.0	0.0	40.59871341579519	0.0
23	3.733558342449438E-4	0.0	0.0	41.983863560843936	0.0
24	3.733558342449438E-4	0.0	0.0	42.93853442900826	0.0
25	3.733558342449438E-4	0.0	0.0	43.63782990654904	0.0
26	3.733558342449438E-4	0.0	0.0	44.258347303064134	0.0
27	3.733558342449438E-4	0.0	0.0	44.788512587691955	0.0
28	3.733558342449438E-4	0.0	0.0	45.28582255890622	0.0
29	3.733558342449438E-4	0.0	0.0	45.82756187439563	0.0
30	3.733558342449438E-4	0.0	0.0	46.37602159490145	0.0
31	3.733558342449438E-4	0.0	0.0	46.86063746775139	0.0
32	3.733558342449438E-4	0.0	0.0	47.261994989564705	0.0
33	3.733558342449438E-4	0.0	0.0	47.69434104562035	0.0
34	3.733558342449438E-4	0.0	0.0	48.032228075612025	0.0
35	3.733558342449438E-4	0.0	0.0	48.38056906896256	0.0
36	3.733558342449438E-4	0.0	0.0	48.71136233810358	0.0
37	3.733558342449438E-4	0.0	0.0	49.04439574225007	0.0
38	3.733558342449438E-4	0.0	0.0	49.36846860637468	0.0
39	3.733558342449438E-4	0.0	0.0	49.70299543385815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	45	3.8198777E-10	45.000004	2
GTTATGG	55	1.8189894E-12	45.000004	2
ACAACGA	20	7.0235884E-4	45.0	13
GTTTGCG	20	7.0235884E-4	45.0	1
GATACTC	20	7.0235884E-4	45.0	9
ACTATGC	20	7.0235884E-4	45.0	33
GGTACAC	25	3.8827682E-5	45.0	8
ATTAGTG	25	3.8827682E-5	45.0	14
ATTAGAC	20	7.0235884E-4	45.0	18
GTACACT	25	3.8827682E-5	45.0	9
CACGGGA	70	0.0	45.0	4
CGACGGT	20	7.0235884E-4	45.0	28
CAACGAG	20	7.0235884E-4	45.0	14
ACGTTGG	20	7.0235884E-4	45.0	2
ACTTGCG	20	7.0235884E-4	45.0	1
CGCATTA	20	7.0235884E-4	45.0	15
TAGGTGC	20	7.0235884E-4	45.0	11
ATGGACC	25	3.8827682E-5	45.0	10
GGACTAT	20	7.0235884E-4	45.0	8
AAGGTCT	20	7.0235884E-4	45.0	34
>>END_MODULE