SRR2935194.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935194.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631308
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17745	2.8108308464331198	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3898	0.6174482186191209	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	3892	0.616497810894207	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2906	0.46031414143334154	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2778	0.44003877663517643	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	1943	0.3077737015846465	Illumina PCR Primer Index 4 (95% over 23bp)
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	1674	0.2651637552510027	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATG	1671	0.2646885513885457	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.23459230676626938	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1364	0.2160593561304466	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1340	0.21225772523079067	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	1263	0.2000608260943945	No Hit
GTTACAGGGAAGTAATACAATTATCTTTATAAATTTTAATTATAAAAATAT	1247	0.19752640549462386	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.17392461365925982	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1055	0.16711335829737622	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1040	0.16473733898509127	No Hit
ACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	889	0.1408187445747559	RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	845	0.13384908792538666	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	791	0.12529541840116076	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGT	670	0.10612886261539534	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	648	0.10264403429071073	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	30	2.1639407E-6	45.000004	2
AACGGGC	45	3.8380676E-10	45.000004	4
CGACCCG	20	7.0308684E-4	45.0	32
CCGCTGA	20	7.0308684E-4	45.0	36
CCGTCAT	20	7.0308684E-4	45.0	22
TAGCACG	35	1.2107557E-7	45.0	1
TCCGTCA	20	7.0308684E-4	45.0	21
ATTGCGG	35	1.2107557E-7	45.0	2
TGCGAAG	25	3.888802E-5	44.999996	1
CGGGCCC	25	3.888802E-5	44.999996	6
CGTTTTT	5835	0.0	43.3419	1
CGTTATT	390	0.0	42.692307	1
TTGACCC	485	0.0	42.680412	8
ACTTGAC	670	0.0	42.64925	6
GCTCAAC	775	0.0	42.387093	1
CTCAACT	740	0.0	41.959457	2
ACGGGTA	70	0.0	41.785713	5
CTTGACC	685	0.0	41.71533	7
GTAATAC	205	0.0	41.707317	12
TTAACGG	65	0.0	41.53846	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content