##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935194.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631308
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6831451526038	31.0	31.0	34.0	30.0	34.0
2	31.790235511034233	33.0	31.0	34.0	30.0	34.0
3	31.786311911143216	33.0	31.0	34.0	30.0	34.0
4	35.58657105564954	37.0	35.0	37.0	33.0	37.0
5	33.04383280427303	37.0	35.0	37.0	28.0	37.0
6	34.07166549449714	37.0	35.0	37.0	28.0	37.0
7	35.417417488769345	37.0	35.0	37.0	32.0	37.0
8	35.399191836631246	37.0	35.0	37.0	33.0	37.0
9	37.473228598402045	39.0	37.0	39.0	35.0	39.0
10	36.86888967033524	39.0	37.0	39.0	32.0	39.0
11	36.775051163615856	39.0	37.0	39.0	32.0	39.0
12	37.093995323993994	39.0	37.0	39.0	33.0	39.0
13	37.035774930778636	39.0	37.0	39.0	33.0	39.0
14	37.938036584361356	40.0	37.0	41.0	33.0	41.0
15	38.10956775456671	40.0	37.0	41.0	33.0	41.0
16	38.112572626990314	40.0	37.0	41.0	33.0	41.0
17	38.080607247175706	40.0	37.0	41.0	33.0	41.0
18	37.9398233508842	39.0	37.0	40.0	33.0	41.0
19	37.734483009877906	39.0	37.0	41.0	33.0	41.0
20	37.52837917466593	39.0	35.0	40.0	33.0	41.0
21	37.45282492856102	39.0	35.0	40.0	33.0	41.0
22	37.437285762258675	39.0	35.0	40.0	33.0	41.0
23	37.19426809101105	39.0	35.0	40.0	33.0	41.0
24	36.91502246130256	39.0	35.0	40.0	32.0	41.0
25	36.74338833026035	38.0	35.0	40.0	32.0	41.0
26	37.05179722100781	39.0	35.0	40.0	33.0	41.0
27	36.993315465668104	39.0	35.0	40.0	33.0	41.0
28	37.06518846585185	39.0	35.0	40.0	33.0	41.0
29	37.10121050263896	39.0	35.0	40.0	33.0	41.0
30	36.83443580629423	39.0	35.0	40.0	32.0	41.0
31	36.685093488439875	39.0	35.0	40.0	31.0	41.0
32	36.418711627288104	39.0	35.0	40.0	31.0	41.0
33	36.214397726624725	39.0	35.0	40.0	30.0	41.0
34	36.06681524707433	39.0	35.0	40.0	30.0	41.0
35	35.80622612100591	39.0	35.0	40.0	27.0	41.0
36	35.66718305486387	39.0	35.0	40.0	26.0	41.0
37	35.649223516888746	39.0	35.0	40.0	26.0	41.0
38	35.48596564592877	39.0	35.0	40.0	25.0	41.0
39	35.349157621953154	39.0	35.0	40.0	24.0	41.0
40	35.253641645599295	39.0	35.0	40.0	24.0	41.0
41	35.161467936411384	39.0	35.0	40.0	23.0	41.0
42	35.250300962446225	39.0	35.0	40.0	23.0	41.0
43	35.15749998415987	39.0	35.0	40.0	23.0	41.0
44	35.06499521628112	39.0	35.0	40.0	22.0	41.0
45	35.021719984540034	39.0	35.0	40.0	23.0	41.0
46	34.88583227204471	38.0	35.0	40.0	22.0	41.0
47	34.82750099792811	38.0	35.0	40.0	22.0	41.0
48	34.77941195106034	38.0	35.0	40.0	22.0	41.0
49	34.796864604915505	38.0	35.0	40.0	22.0	41.0
50	34.707087507207255	38.0	35.0	40.0	20.0	41.0
51	33.761781887763185	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	10.0
10	21.0
11	9.0
12	30.0
13	26.0
14	43.0
15	61.0
16	98.0
17	181.0
18	295.0
19	557.0
20	959.0
21	1572.0
22	2336.0
23	3634.0
24	5300.0
25	7837.0
26	10420.0
27	11669.0
28	11377.0
29	11490.0
30	12048.0
31	14117.0
32	17478.0
33	24250.0
34	35104.0
35	49855.0
36	65333.0
37	86902.0
38	136541.0
39	121728.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.482693075329315	22.91401344510128	23.383831663783763	19.21946181578564
2	37.114372065616145	23.240953702471696	24.686999055928325	14.95767517598383
3	26.44477814315675	23.000025344205998	37.15634840679985	13.398848105837406
4	23.83448332668048	24.653576384268852	35.00272450214476	16.509215786905916
5	17.714332782096854	33.958701616326735	32.525803569731416	15.801162031844996
6	21.07259847808043	33.04963662744651	33.81788287175198	12.05988202272108
7	67.1070222458768	6.719857819004353	20.496651396782553	5.676468538336279
8	65.70738846965348	6.087678280649065	21.955052050663067	6.249881199034386
9	59.531797474449874	9.815335779049212	24.303192736350564	6.3496740101503555
10	23.614463938362892	37.816089769177644	27.79704993442187	10.772396358037598
11	19.20251287802467	24.919215343382312	38.08457995146585	17.79369182712717
12	19.987708060091112	21.143245452299038	40.31978051917606	18.549265968433794
13	20.557635892464535	21.784612265328494	42.54706102251199	15.110690819694984
14	15.178644972026333	25.48708395901842	40.709447686390796	18.624823382564454
15	13.19403524111844	25.81671703827609	43.6066072345036	17.38264048610187
16	15.318038105013718	26.28606005309611	39.74715986491538	18.64874197697479
17	14.98175217168165	26.296831340645138	37.43656028436199	21.28485620331122
18	14.042274135604174	26.105482585362456	42.10448782527704	17.747755453756326
19	14.438118953030852	27.223795675011246	40.07235770812345	18.265727663834454
20	14.29524099171878	28.989336425326467	40.61614932806174	16.099273254893014
21	14.63105172118839	27.14839666216807	41.8573818167994	16.363169799844133
22	13.602393760256485	25.560423755124283	38.83112521938578	22.00605726523345
23	12.512117698492652	26.04845812186761	40.45014477877677	20.98927940086297
24	14.73844779410367	26.243608508049952	38.22809151792786	20.78985217991852
25	12.989380777686963	28.013742895702254	36.41503038136694	22.581845945243842
26	12.124192945440262	27.76045923701268	38.35845577752856	21.756892040018503
27	14.218099564713263	25.869781469583785	39.761574382076574	20.150544583626377
28	10.982278063956104	25.934725997452908	39.27544083078307	23.807555107807914
29	12.611752108321136	25.818776255013397	37.28354464065084	24.285926996014624
30	15.306158008452291	27.85502480564162	36.84112984470338	19.99768734120271
31	14.488648963738775	29.701033409999557	33.26316156297718	22.547156063284483
32	14.03688849182966	30.14297300208456	32.64317892375829	23.176959582327488
33	14.465205573190898	28.332763088698385	33.313216369822655	23.888814968288063
34	15.701686023304	25.38507352987765	32.742021327149345	26.171219119669004
35	13.962439886711401	27.027219677241536	33.533235758140236	25.47710467790682
36	14.602381088153486	26.861848733106502	33.54717507143898	24.98859510730103
37	15.395496334594208	27.291591426055113	34.49995881566525	22.81295342368543
38	14.182300873741502	30.700228731459127	31.534528312646128	23.582942082153245
39	18.2961407110317	28.333555095135814	30.117945598661827	23.252358595170662
40	19.896944122361827	25.75858376576885	34.07829458837842	20.26617752349091
41	17.173233984045822	24.358949989545515	32.92323240003295	25.544583626375715
42	16.436192793375024	23.31445189986504	34.53274788217479	25.71660742458515
43	17.088172492666022	23.64630259714751	34.010340436047066	25.25518447413941
44	17.185747685757192	25.058450075082213	30.30755193978217	27.448250299378437
45	18.44598832899314	24.088875794382457	31.320844975827967	26.14429090079644
46	19.830257180330364	24.29226304751405	33.42948291483713	22.447996857318454
47	15.62818782591065	24.59987834781121	37.20846243038263	22.563471395895508
48	14.615845197589767	24.008883144202194	35.81785752754598	25.557414130662053
49	18.730477041317393	22.17269541966837	35.99906860042958	23.097758938584654
50	17.54341779289982	21.202487533818676	35.40078693759623	25.853307735685277
51	17.584918930221065	20.610541922484746	31.771338237437195	30.033200909856994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5839.0
1	7208.5
2	8578.0
3	18303.0
4	28028.0
5	17776.0
6	7524.0
7	7439.5
8	7355.0
9	7511.5
10	7668.0
11	7721.5
12	7775.0
13	7744.5
14	7714.0
15	7664.0
16	7614.0
17	6962.0
18	6310.0
19	6022.0
20	5734.0
21	6391.0
22	7048.0
23	6597.0
24	6146.0
25	7069.5
26	8264.5
27	8536.0
28	9416.0
29	10296.0
30	10989.0
31	11682.0
32	11769.0
33	11856.0
34	13028.0
35	14200.0
36	15241.5
37	16283.0
38	17383.0
39	18483.0
40	20831.0
41	23179.0
42	27451.5
43	31724.0
44	36309.5
45	40895.0
46	51318.5
47	61742.0
48	71327.5
49	80913.0
50	76389.0
51	71865.0
52	56366.0
53	40867.0
54	31294.5
55	21722.0
56	17287.5
57	12853.0
58	11238.5
59	9624.0
60	8580.0
61	7536.0
62	6504.0
63	5472.0
64	4772.5
65	4073.0
66	3322.0
67	2571.0
68	2044.0
69	1517.0
70	1208.5
71	900.0
72	726.0
73	552.0
74	438.0
75	235.0
76	146.0
77	119.0
78	92.0
79	66.5
80	41.0
81	30.5
82	20.0
83	15.5
84	11.0
85	7.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	631308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.69545376577323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.07879688831429	30.053581431492677
2	11.334297019741618	9.45177314708356
3	4.892703903356249	6.120105281760262
4	2.764841546968854	4.611252915653152
5	1.8922489208001616	3.9449088695278722
6	1.3654747177663564	3.4160452777775623
7	1.048710569217236	3.060852413673249
8	0.8215071187820542	2.7402489671544576
9	0.6832685819822936	2.5640274212683347
>10	2.96927341908084	19.117517883676264
>50	0.0890919361842087	2.503857485525374
>100	0.049625771119904113	3.790815447045606
>500	0.003907541033063316	1.06602937508142
>1k	0.005861311549594973	4.66784985420796
>5k	0.0	0.0
>10k+	3.907541033063315E-4	2.8911342290722604
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17745	2.8108308464331198	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3898	0.6174482186191209	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	3892	0.616497810894207	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2906	0.46031414143334154	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2778	0.44003877663517643	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	1943	0.3077737015846465	Illumina PCR Primer Index 4 (95% over 23bp)
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	1674	0.2651637552510027	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATG	1671	0.2646885513885457	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.23459230676626938	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1364	0.2160593561304466	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1340	0.21225772523079067	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	1263	0.2000608260943945	No Hit
GTTACAGGGAAGTAATACAATTATCTTTATAAATTTTAATTATAAAAATAT	1247	0.19752640549462386	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.17392461365925982	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1055	0.16711335829737622	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1040	0.16473733898509127	No Hit
ACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	889	0.1408187445747559	RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	845	0.13384908792538666	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	791	0.12529541840116076	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGT	670	0.10612886261539534	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	648	0.10264403429071073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5840128748566469E-4	0.0	0.0	0.33501872303218083	0.0
2	1.5840128748566469E-4	0.0	0.0	1.6923593554968415	0.0
3	1.5840128748566469E-4	0.0	0.0	2.324222091277158	0.0
4	1.5840128748566469E-4	0.0	0.0	3.230752659557617	0.0
5	1.5840128748566469E-4	0.0	0.0	5.674567722886452	0.0
6	1.5840128748566469E-4	0.0	0.0	6.999119288841579	0.0
7	1.5840128748566469E-4	0.0	0.0	8.336501359083046	0.0
8	1.5840128748566469E-4	0.0	0.0	10.586433246529428	0.0
9	1.5840128748566469E-4	0.0	0.0	11.405367902830314	0.0
10	1.5840128748566469E-4	0.0	0.0	13.816710702224588	0.0
11	1.5840128748566469E-4	0.0	0.0	17.664911580401327	0.0
12	1.5840128748566469E-4	0.0	0.0	20.65172625723102	0.0
13	1.5840128748566469E-4	0.0	0.0	21.99259315579717	0.0
14	3.1680257497132937E-4	0.0	0.0	22.42011823072098	0.0
15	3.1680257497132937E-4	0.0	0.0	23.578190043528675	0.0
16	3.1680257497132937E-4	0.0	0.0	25.309991319609445	0.0
17	3.1680257497132937E-4	0.0	0.0	27.511452413085212	0.0
18	3.1680257497132937E-4	0.0	0.0	29.659849075253284	0.0
19	3.1680257497132937E-4	0.0	0.0	31.076748591812553	0.0
20	3.1680257497132937E-4	0.0	0.0	32.66979034005588	0.0
21	3.1680257497132937E-4	0.0	0.0	34.43960792513322	0.0
22	3.1680257497132937E-4	0.0	0.0	36.38382532773226	0.0
23	3.1680257497132937E-4	0.0	0.0	37.907804114631844	0.0
24	3.1680257497132937E-4	0.0	0.0	38.96085587383654	0.0
25	3.1680257497132937E-4	0.0	0.0	39.895106667426994	0.0
26	3.1680257497132937E-4	0.0	0.0	40.645136763671616	0.0
27	3.1680257497132937E-4	0.0	0.0	41.34970569040785	0.0
28	3.1680257497132937E-4	0.0	0.0	41.945136130066466	0.0
29	3.1680257497132937E-4	0.0	0.0	42.57779087228421	0.0
30	3.1680257497132937E-4	0.0	0.0	43.24830352221103	0.0
31	4.7520386245699406E-4	0.0	0.0	43.811420099222566	0.0
32	4.7520386245699406E-4	0.0	0.0	44.310859358664864	0.0
33	4.7520386245699406E-4	0.0	0.0	44.749472523712676	0.0
34	4.7520386245699406E-4	0.0	0.0	45.245426954830286	0.0
35	4.7520386245699406E-4	0.0	0.0	45.73187730869876	0.0
36	4.7520386245699406E-4	0.0	0.0	46.16066959392246	0.0
37	4.7520386245699406E-4	0.0	0.0	46.55017835984971	0.0
38	4.7520386245699406E-4	0.0	0.0	46.968674561386834	0.0
39	4.7520386245699406E-4	0.0	0.0	47.44942246890583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	30	2.1639407E-6	45.000004	2
AACGGGC	45	3.8380676E-10	45.000004	4
CGACCCG	20	7.0308684E-4	45.0	32
CCGCTGA	20	7.0308684E-4	45.0	36
CCGTCAT	20	7.0308684E-4	45.0	22
TAGCACG	35	1.2107557E-7	45.0	1
TCCGTCA	20	7.0308684E-4	45.0	21
ATTGCGG	35	1.2107557E-7	45.0	2
TGCGAAG	25	3.888802E-5	44.999996	1
CGGGCCC	25	3.888802E-5	44.999996	6
CGTTTTT	5835	0.0	43.3419	1
CGTTATT	390	0.0	42.692307	1
TTGACCC	485	0.0	42.680412	8
ACTTGAC	670	0.0	42.64925	6
GCTCAAC	775	0.0	42.387093	1
CTCAACT	740	0.0	41.959457	2
ACGGGTA	70	0.0	41.785713	5
CTTGACC	685	0.0	41.71533	7
GTAATAC	205	0.0	41.707317	12
TTAACGG	65	0.0	41.53846	2
>>END_MODULE