SRR2935193.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412397
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8951	2.17048135655691	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6248	1.5150449688043317	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3715	0.9008309953758151	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	3574	0.8666406399658582	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3245	0.7868631440092919	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	2515	0.609849247205969	Illumina Single End Adapter 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2458	0.5960276141679014	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1662	0.40300972121523676	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	1194	0.2895268394289968	No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1157	0.2805549021937599	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	772	0.18719825798926762	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.17240668579063378	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	655	0.15882753754270762	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.158100083172283	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	637	0.15446281132015996	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	614	0.14888566114690455	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	590	0.14306602618350764	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.14039869349195072	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	569	0.13797384559053533	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	513	0.12439469734260918	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGT	510	0.12366724297218457	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	501	0.12148487986091072	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	413	0.10014621832845534	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.028018E-4	45.000004	1
TCTGATT	20	7.028018E-4	45.000004	43
GGAGCGA	20	7.028018E-4	45.000004	9
CATATGG	40	6.7975634E-9	45.000004	2
GGTAATC	20	7.028018E-4	45.000004	8
GTACACG	20	7.028018E-4	45.000004	1
CCGATGA	20	7.028018E-4	45.000004	18
GGATCCG	20	7.028018E-4	45.000004	8
GTCTACG	20	7.028018E-4	45.000004	1
GAGGTGC	20	7.028018E-4	45.000004	9
TACGAAT	20	7.028018E-4	45.000004	12
CTGCAAC	20	7.028018E-4	45.000004	21
CGAATAT	20	7.028018E-4	45.000004	14
TCGTAAG	20	7.028018E-4	45.000004	1
CCTGGAC	20	7.028018E-4	45.000004	11
TAGGCAT	20	7.028018E-4	45.000004	24
GCTGTTG	20	7.028018E-4	45.000004	1
GGCGAAG	20	7.028018E-4	45.000004	1
TGTACGG	20	7.028018E-4	45.000004	2
CTAATGT	20	7.028018E-4	45.000004	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content