##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935193.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 412397 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.544599015026783 31.0 31.0 34.0 30.0 34.0 2 31.655426688360972 31.0 31.0 34.0 30.0 34.0 3 31.669207098984717 31.0 31.0 34.0 30.0 34.0 4 35.49812922984406 37.0 35.0 37.0 33.0 37.0 5 32.938311869387995 37.0 35.0 37.0 28.0 37.0 6 33.96233241269941 37.0 35.0 37.0 27.0 37.0 7 35.283202836102106 37.0 35.0 37.0 32.0 37.0 8 35.25051588639103 37.0 35.0 37.0 33.0 37.0 9 37.31479375456175 39.0 37.0 39.0 34.0 39.0 10 36.736050456235134 39.0 37.0 39.0 32.0 39.0 11 36.5845435345068 39.0 35.0 39.0 32.0 39.0 12 36.997126555236825 39.0 37.0 39.0 33.0 39.0 13 36.961878966141846 39.0 37.0 39.0 33.0 39.0 14 37.91225445383938 40.0 37.0 41.0 33.0 41.0 15 38.02234012371574 40.0 37.0 41.0 33.0 41.0 16 38.002560639383894 40.0 37.0 41.0 33.0 41.0 17 37.9888626735888 39.0 37.0 41.0 33.0 41.0 18 37.85004255608067 39.0 37.0 40.0 33.0 41.0 19 37.572719976139496 39.0 36.0 40.0 33.0 41.0 20 37.378698196155646 39.0 35.0 40.0 33.0 41.0 21 37.30278105805813 39.0 35.0 40.0 33.0 41.0 22 37.23800367121972 39.0 35.0 40.0 33.0 41.0 23 36.981559031709736 39.0 35.0 40.0 32.0 41.0 24 36.68187207957381 38.0 35.0 40.0 31.0 41.0 25 36.50199928709472 38.0 35.0 40.0 31.0 41.0 26 36.834853308826204 38.0 35.0 40.0 32.0 41.0 27 36.755403167336326 39.0 35.0 40.0 32.0 41.0 28 36.8393053295732 39.0 35.0 40.0 32.0 41.0 29 36.901665143053904 39.0 35.0 40.0 33.0 41.0 30 36.63982521696327 39.0 35.0 40.0 31.0 41.0 31 36.411363322235616 38.0 35.0 40.0 31.0 41.0 32 36.11475350208658 38.0 35.0 40.0 30.0 41.0 33 35.86781911604594 38.0 35.0 40.0 29.0 41.0 34 35.664265258961635 39.0 35.0 40.0 27.0 41.0 35 35.48234831970165 39.0 35.0 40.0 25.0 41.0 36 35.32104501245159 39.0 35.0 40.0 24.0 41.0 37 35.25005031559395 38.0 35.0 40.0 24.0 41.0 38 35.14621347875954 38.0 35.0 40.0 24.0 41.0 39 35.01893806211005 38.0 35.0 40.0 23.0 41.0 40 34.916112386850536 38.0 35.0 40.0 23.0 41.0 41 34.819370654975664 38.0 35.0 40.0 22.0 41.0 42 34.87918195331198 38.0 35.0 40.0 22.0 41.0 43 34.805134372946455 38.0 35.0 40.0 21.0 41.0 44 34.73370077861866 38.0 35.0 40.0 21.0 41.0 45 34.74074496177227 38.0 35.0 40.0 20.0 41.0 46 34.59583847603159 38.0 35.0 40.0 20.0 41.0 47 34.481749382269996 38.0 34.0 40.0 20.0 41.0 48 34.43692606881233 38.0 34.0 40.0 20.0 41.0 49 34.447753014692154 38.0 34.0 40.0 20.0 41.0 50 34.362587506698645 38.0 34.0 40.0 18.0 41.0 51 33.436909094877024 37.0 33.0 40.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 2.0 11 15.0 12 17.0 13 12.0 14 20.0 15 39.0 16 69.0 17 132.0 18 215.0 19 423.0 20 718.0 21 1157.0 22 1813.0 23 2677.0 24 3962.0 25 5863.0 26 7652.0 27 8165.0 28 7418.0 29 7410.0 30 8093.0 31 9630.0 32 12138.0 33 15948.0 34 23350.0 35 36468.0 36 45385.0 37 60344.0 38 88772.0 39 64476.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.508260244376174 23.88038710271898 22.304235966798984 18.307116686105864 2 37.1295135512625 25.63573449855358 22.714520231718467 14.52023171846546 3 25.495335805061625 24.968416356084063 36.18018559785837 13.356062240995934 4 23.73174392636222 26.064447607523817 34.9730963125338 15.230712153580168 5 17.512494028812043 33.254849089590856 33.286614597099394 15.946042284497706 6 19.412119874780853 33.845057068795356 34.221393463095026 12.521429593328758 7 62.055495069071796 9.319660424299887 22.678389997987374 5.9464545086409455 8 60.074636818405565 8.498849409670777 22.6633559409986 8.763157830925056 9 54.75840027934248 10.99717020249905 25.70678254206505 8.537646976093423 10 25.889616073831768 28.154181528963594 32.420216441923685 13.535985955280955 11 17.267584390769088 26.40562370725296 35.64671905954699 20.680072842430956 12 16.743817244063365 22.693424054976152 42.41786433945931 18.144894361501173 13 18.036018690727623 25.65877055361702 42.72557753814892 13.579633217506432 14 14.819215464709975 28.078283789649294 38.97482280423961 18.127677941401128 15 12.1433958054981 28.204375880522893 42.305351396833636 17.34687691714537 16 12.019486077735774 29.479845876667387 37.589507198160995 20.911160847435845 17 12.308285462794347 29.318108521642984 35.911027480801266 22.462578534761406 18 12.465415606806063 27.502624897853284 42.019704314046905 18.012255181293753 19 12.574291277579613 29.851817544744506 38.16928833138941 19.404602846286465 20 13.40261932070311 31.355708213202327 38.82278484082086 16.418887625273705 21 13.855580908687504 30.7618629621457 39.266532006779876 16.116024122386925 22 10.884414775083233 29.514036232077345 35.511170061857875 24.090378930981554 23 11.373021627218433 28.778337378787917 36.72407898214585 23.124562011847807 24 15.530423354195108 27.143504923653666 35.42387553740692 21.902196184744312 25 11.562644733109115 30.60570275729455 33.08753458439319 24.744117925203142 26 11.173456644931946 29.273976289837222 36.900365424578744 22.652201640652088 27 14.450638583694838 27.909756860500924 37.60575367910048 20.03385087670376 28 9.568934788565388 27.249228292155376 36.321069260930614 26.860767658348628 29 11.81701127796759 26.998499019149026 34.878527244378596 26.30596245850479 30 14.622560299905189 28.391089168931877 33.86712318469824 23.119227346464694 31 11.994025174770913 34.08075228481294 28.486143206667364 25.439079333748793 32 13.642921747733375 33.94908304376608 27.651268074210044 24.7567271342905 33 13.619400723089644 31.13577450854395 28.852537724571224 26.392287043795175 34 12.258818565605473 30.624374086135447 28.59283651432964 28.52397083392944 35 14.618923028053066 28.985904359149075 27.786574587109026 28.608598025688835 36 14.577215644148719 30.492947329878735 30.059869494685948 24.869967531286598 37 13.091996304531797 32.65227438608913 31.660996563990523 22.594732745388548 38 15.003503905217544 34.176533776918845 25.611728504329566 25.208233813534047 39 16.471264339944277 31.829038523558612 27.340402573248596 24.359294563248522 40 19.019779484331846 29.133820081135408 28.998756053026575 22.84764438150617 41 18.423024415793517 30.92360031717011 25.58893493405626 25.064440332980116 42 18.680543262923834 29.097932332194464 29.60011833257759 22.621406072304115 43 18.386409212482146 27.156356617531163 31.129469904000274 23.327764265986417 44 15.601471397706579 26.411928311796647 28.313251551296442 29.673348739200335 45 18.13155769804339 24.45507605535443 27.823674760000678 29.589691486601506 46 20.239235493953643 26.102517719576042 30.17529225479332 23.482954531677 47 14.206213915232166 28.737357449254 34.033710235525476 23.02271839998836 48 15.671549502057482 26.949517091540432 31.869048513931965 25.50988489247012 49 18.47321876735282 24.266665373414455 34.348697977919336 22.911417881313394 50 17.58184467879252 22.658021275615486 32.068855981008596 27.691278064583397 51 16.535037839751503 21.869703222865347 28.021784833546313 33.57347410383684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6969.0 1 6986.0 2 7003.0 3 10885.5 4 14768.0 5 9781.5 6 4795.0 7 4835.0 8 4875.0 9 5364.0 10 5853.0 11 6217.0 12 6581.0 13 6763.0 14 6945.0 15 6485.0 16 6025.0 17 5219.0 18 4413.0 19 4319.0 20 4225.0 21 3997.0 22 3769.0 23 3433.0 24 3097.0 25 3058.0 26 3383.5 27 3748.0 28 4047.5 29 4347.0 30 4598.5 31 4850.0 32 5046.5 33 5243.0 34 5938.0 35 6633.0 36 7037.0 37 7441.0 38 8229.0 39 9017.0 40 10429.5 41 11842.0 42 15945.0 43 20048.0 44 24107.5 45 28167.0 46 36941.5 47 45716.0 48 54542.5 49 63369.0 50 59890.5 51 56412.0 52 43364.0 53 30316.0 54 22458.0 55 14600.0 56 10844.0 57 7088.0 58 5578.0 59 4068.0 60 3372.0 61 2676.0 62 2277.5 63 1879.0 64 1490.5 65 1102.0 66 885.0 67 668.0 68 498.0 69 328.0 70 263.0 71 198.0 72 169.0 73 140.0 74 114.0 75 63.0 76 38.0 77 32.0 78 26.0 79 17.0 80 8.0 81 5.5 82 3.0 83 1.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 412397.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.952823452774815 #Duplication Level Percentage of deduplicated Percentage of total 1 73.46295199504661 30.81978255367862 2 11.281169779396613 9.465538481916095 3 4.842371621864972 6.09453485234484 4 2.5920722816704957 4.349790032390141 5 1.6858767177771197 3.5363644152023483 6 1.2483603655929445 3.1423345213897336 7 0.8913443121989576 2.6176087395722503 8 0.6904499092462107 2.3173058516472542 9 0.5622184445064196 2.122798602984434 >10 2.5595475582409977 17.260769853294818 >50 0.10877861366021642 3.1370863275115632 >100 0.06199211316119859 4.825852571236113 >500 0.007017975074852671 1.7918145979834932 >1k 0.004678650049901781 4.78929494079663 >5k 0.0011696625124754452 3.7291236580516385 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8951 2.17048135655691 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6248 1.5150449688043317 No Hit GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC 3715 0.9008309953758151 Illumina Single End Adapter 2 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC 3574 0.8666406399658582 No Hit CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC 3245 0.7868631440092919 Illumina Single End Adapter 2 (95% over 21bp) CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT 2515 0.609849247205969 Illumina Single End Adapter 2 (95% over 22bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2458 0.5960276141679014 No Hit TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC 1662 0.40300972121523676 Illumina Single End Adapter 2 (95% over 21bp) CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG 1194 0.2895268394289968 No Hit ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC 1157 0.2805549021937599 Illumina Single End Adapter 2 (95% over 21bp) CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC 772 0.18719825798926762 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.17240668579063378 No Hit ACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG 655 0.15882753754270762 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 652 0.158100083172283 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.15446281132015996 No Hit AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG 614 0.14888566114690455 No Hit CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG 590 0.14306602618350764 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 579 0.14039869349195072 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC 569 0.13797384559053533 No Hit GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG 513 0.12439469734260918 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGT 510 0.12366724297218457 No Hit GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC 501 0.12148487986091072 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG 413 0.10014621832845534 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.8496958028307677E-4 0.0 0.0 0.6505866919497475 0.0 2 4.8496958028307677E-4 0.0 0.0 3.196434503645759 0.0 3 4.8496958028307677E-4 0.0 0.0 4.289798422393956 0.0 4 4.8496958028307677E-4 0.0 0.0 5.742767284922054 0.0 5 4.8496958028307677E-4 0.0 0.0 9.391920891762064 0.0 6 4.8496958028307677E-4 0.0 0.0 11.289606859409744 0.0 7 4.8496958028307677E-4 0.0 0.0 13.253733659556204 0.0 8 4.8496958028307677E-4 0.0 0.0 16.24793584822392 0.0 9 4.8496958028307677E-4 0.0 0.0 17.222967189383045 0.0 10 4.8496958028307677E-4 0.0 0.0 20.259846701115674 0.0 11 4.8496958028307677E-4 0.0 0.0 25.312502273294907 0.0 12 4.8496958028307677E-4 0.0 0.0 28.80088846427108 0.0 13 4.8496958028307677E-4 0.0 0.0 30.031741259029527 0.0 14 4.8496958028307677E-4 0.0 0.0 30.499979388792838 0.0 15 4.8496958028307677E-4 0.0 0.0 31.43839552664059 0.0 16 4.8496958028307677E-4 0.0 0.0 33.46484091785343 0.0 17 4.8496958028307677E-4 0.0 0.0 36.03930193478614 0.0 18 4.8496958028307677E-4 0.0 0.0 38.76337606723618 0.0 19 4.8496958028307677E-4 0.0 0.0 40.49399001447634 0.0 20 4.8496958028307677E-4 0.0 0.0 42.06504896980337 0.0 21 4.8496958028307677E-4 0.0 0.0 43.93921391280732 0.0 22 4.8496958028307677E-4 0.0 0.0 45.85702611803675 0.0 23 4.8496958028307677E-4 0.0 0.0 47.65141356508413 0.0 24 4.8496958028307677E-4 0.0 0.0 48.80515619657757 0.0 25 4.8496958028307677E-4 0.0 0.0 49.68925574143362 0.0 26 4.8496958028307677E-4 0.0 0.0 50.46714694820767 0.0 27 4.8496958028307677E-4 0.0 0.0 51.1182186097377 0.0 28 4.8496958028307677E-4 0.0 0.0 51.74528427704372 0.0 29 4.8496958028307677E-4 0.0 0.0 52.421816841538615 0.0 30 4.8496958028307677E-4 0.0 0.0 53.02487651462062 0.0 31 4.8496958028307677E-4 0.0 0.0 53.566830020586956 0.0 32 4.8496958028307677E-4 0.0 0.0 54.00136276452059 0.0 33 4.8496958028307677E-4 0.0 0.0 54.43662296282466 0.0 34 4.8496958028307677E-4 0.0 0.0 54.8367228665582 0.0 35 4.8496958028307677E-4 0.0 0.0 55.25694900787348 0.0 36 4.8496958028307677E-4 0.0 0.0 55.64419721772952 0.0 37 4.8496958028307677E-4 0.0 0.0 56.02950554926442 0.0 38 4.8496958028307677E-4 0.0 0.0 56.34594820039913 0.0 39 4.8496958028307677E-4 0.0 0.0 56.68639684575785 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAGCG 20 7.028018E-4 45.000004 1 TCTGATT 20 7.028018E-4 45.000004 43 GGAGCGA 20 7.028018E-4 45.000004 9 CATATGG 40 6.7975634E-9 45.000004 2 GGTAATC 20 7.028018E-4 45.000004 8 GTACACG 20 7.028018E-4 45.000004 1 CCGATGA 20 7.028018E-4 45.000004 18 GGATCCG 20 7.028018E-4 45.000004 8 GTCTACG 20 7.028018E-4 45.000004 1 GAGGTGC 20 7.028018E-4 45.000004 9 TACGAAT 20 7.028018E-4 45.000004 12 CTGCAAC 20 7.028018E-4 45.000004 21 CGAATAT 20 7.028018E-4 45.000004 14 TCGTAAG 20 7.028018E-4 45.000004 1 CCTGGAC 20 7.028018E-4 45.000004 11 TAGGCAT 20 7.028018E-4 45.000004 24 GCTGTTG 20 7.028018E-4 45.000004 1 GGCGAAG 20 7.028018E-4 45.000004 1 TGTACGG 20 7.028018E-4 45.000004 2 CTAATGT 20 7.028018E-4 45.000004 16 >>END_MODULE