##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412397
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.544599015026783	31.0	31.0	34.0	30.0	34.0
2	31.655426688360972	31.0	31.0	34.0	30.0	34.0
3	31.669207098984717	31.0	31.0	34.0	30.0	34.0
4	35.49812922984406	37.0	35.0	37.0	33.0	37.0
5	32.938311869387995	37.0	35.0	37.0	28.0	37.0
6	33.96233241269941	37.0	35.0	37.0	27.0	37.0
7	35.283202836102106	37.0	35.0	37.0	32.0	37.0
8	35.25051588639103	37.0	35.0	37.0	33.0	37.0
9	37.31479375456175	39.0	37.0	39.0	34.0	39.0
10	36.736050456235134	39.0	37.0	39.0	32.0	39.0
11	36.5845435345068	39.0	35.0	39.0	32.0	39.0
12	36.997126555236825	39.0	37.0	39.0	33.0	39.0
13	36.961878966141846	39.0	37.0	39.0	33.0	39.0
14	37.91225445383938	40.0	37.0	41.0	33.0	41.0
15	38.02234012371574	40.0	37.0	41.0	33.0	41.0
16	38.002560639383894	40.0	37.0	41.0	33.0	41.0
17	37.9888626735888	39.0	37.0	41.0	33.0	41.0
18	37.85004255608067	39.0	37.0	40.0	33.0	41.0
19	37.572719976139496	39.0	36.0	40.0	33.0	41.0
20	37.378698196155646	39.0	35.0	40.0	33.0	41.0
21	37.30278105805813	39.0	35.0	40.0	33.0	41.0
22	37.23800367121972	39.0	35.0	40.0	33.0	41.0
23	36.981559031709736	39.0	35.0	40.0	32.0	41.0
24	36.68187207957381	38.0	35.0	40.0	31.0	41.0
25	36.50199928709472	38.0	35.0	40.0	31.0	41.0
26	36.834853308826204	38.0	35.0	40.0	32.0	41.0
27	36.755403167336326	39.0	35.0	40.0	32.0	41.0
28	36.8393053295732	39.0	35.0	40.0	32.0	41.0
29	36.901665143053904	39.0	35.0	40.0	33.0	41.0
30	36.63982521696327	39.0	35.0	40.0	31.0	41.0
31	36.411363322235616	38.0	35.0	40.0	31.0	41.0
32	36.11475350208658	38.0	35.0	40.0	30.0	41.0
33	35.86781911604594	38.0	35.0	40.0	29.0	41.0
34	35.664265258961635	39.0	35.0	40.0	27.0	41.0
35	35.48234831970165	39.0	35.0	40.0	25.0	41.0
36	35.32104501245159	39.0	35.0	40.0	24.0	41.0
37	35.25005031559395	38.0	35.0	40.0	24.0	41.0
38	35.14621347875954	38.0	35.0	40.0	24.0	41.0
39	35.01893806211005	38.0	35.0	40.0	23.0	41.0
40	34.916112386850536	38.0	35.0	40.0	23.0	41.0
41	34.819370654975664	38.0	35.0	40.0	22.0	41.0
42	34.87918195331198	38.0	35.0	40.0	22.0	41.0
43	34.805134372946455	38.0	35.0	40.0	21.0	41.0
44	34.73370077861866	38.0	35.0	40.0	21.0	41.0
45	34.74074496177227	38.0	35.0	40.0	20.0	41.0
46	34.59583847603159	38.0	35.0	40.0	20.0	41.0
47	34.481749382269996	38.0	34.0	40.0	20.0	41.0
48	34.43692606881233	38.0	34.0	40.0	20.0	41.0
49	34.447753014692154	38.0	34.0	40.0	20.0	41.0
50	34.362587506698645	38.0	34.0	40.0	18.0	41.0
51	33.436909094877024	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	15.0
12	17.0
13	12.0
14	20.0
15	39.0
16	69.0
17	132.0
18	215.0
19	423.0
20	718.0
21	1157.0
22	1813.0
23	2677.0
24	3962.0
25	5863.0
26	7652.0
27	8165.0
28	7418.0
29	7410.0
30	8093.0
31	9630.0
32	12138.0
33	15948.0
34	23350.0
35	36468.0
36	45385.0
37	60344.0
38	88772.0
39	64476.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.508260244376174	23.88038710271898	22.304235966798984	18.307116686105864
2	37.1295135512625	25.63573449855358	22.714520231718467	14.52023171846546
3	25.495335805061625	24.968416356084063	36.18018559785837	13.356062240995934
4	23.73174392636222	26.064447607523817	34.9730963125338	15.230712153580168
5	17.512494028812043	33.254849089590856	33.286614597099394	15.946042284497706
6	19.412119874780853	33.845057068795356	34.221393463095026	12.521429593328758
7	62.055495069071796	9.319660424299887	22.678389997987374	5.9464545086409455
8	60.074636818405565	8.498849409670777	22.6633559409986	8.763157830925056
9	54.75840027934248	10.99717020249905	25.70678254206505	8.537646976093423
10	25.889616073831768	28.154181528963594	32.420216441923685	13.535985955280955
11	17.267584390769088	26.40562370725296	35.64671905954699	20.680072842430956
12	16.743817244063365	22.693424054976152	42.41786433945931	18.144894361501173
13	18.036018690727623	25.65877055361702	42.72557753814892	13.579633217506432
14	14.819215464709975	28.078283789649294	38.97482280423961	18.127677941401128
15	12.1433958054981	28.204375880522893	42.305351396833636	17.34687691714537
16	12.019486077735774	29.479845876667387	37.589507198160995	20.911160847435845
17	12.308285462794347	29.318108521642984	35.911027480801266	22.462578534761406
18	12.465415606806063	27.502624897853284	42.019704314046905	18.012255181293753
19	12.574291277579613	29.851817544744506	38.16928833138941	19.404602846286465
20	13.40261932070311	31.355708213202327	38.82278484082086	16.418887625273705
21	13.855580908687504	30.7618629621457	39.266532006779876	16.116024122386925
22	10.884414775083233	29.514036232077345	35.511170061857875	24.090378930981554
23	11.373021627218433	28.778337378787917	36.72407898214585	23.124562011847807
24	15.530423354195108	27.143504923653666	35.42387553740692	21.902196184744312
25	11.562644733109115	30.60570275729455	33.08753458439319	24.744117925203142
26	11.173456644931946	29.273976289837222	36.900365424578744	22.652201640652088
27	14.450638583694838	27.909756860500924	37.60575367910048	20.03385087670376
28	9.568934788565388	27.249228292155376	36.321069260930614	26.860767658348628
29	11.81701127796759	26.998499019149026	34.878527244378596	26.30596245850479
30	14.622560299905189	28.391089168931877	33.86712318469824	23.119227346464694
31	11.994025174770913	34.08075228481294	28.486143206667364	25.439079333748793
32	13.642921747733375	33.94908304376608	27.651268074210044	24.7567271342905
33	13.619400723089644	31.13577450854395	28.852537724571224	26.392287043795175
34	12.258818565605473	30.624374086135447	28.59283651432964	28.52397083392944
35	14.618923028053066	28.985904359149075	27.786574587109026	28.608598025688835
36	14.577215644148719	30.492947329878735	30.059869494685948	24.869967531286598
37	13.091996304531797	32.65227438608913	31.660996563990523	22.594732745388548
38	15.003503905217544	34.176533776918845	25.611728504329566	25.208233813534047
39	16.471264339944277	31.829038523558612	27.340402573248596	24.359294563248522
40	19.019779484331846	29.133820081135408	28.998756053026575	22.84764438150617
41	18.423024415793517	30.92360031717011	25.58893493405626	25.064440332980116
42	18.680543262923834	29.097932332194464	29.60011833257759	22.621406072304115
43	18.386409212482146	27.156356617531163	31.129469904000274	23.327764265986417
44	15.601471397706579	26.411928311796647	28.313251551296442	29.673348739200335
45	18.13155769804339	24.45507605535443	27.823674760000678	29.589691486601506
46	20.239235493953643	26.102517719576042	30.17529225479332	23.482954531677
47	14.206213915232166	28.737357449254	34.033710235525476	23.02271839998836
48	15.671549502057482	26.949517091540432	31.869048513931965	25.50988489247012
49	18.47321876735282	24.266665373414455	34.348697977919336	22.911417881313394
50	17.58184467879252	22.658021275615486	32.068855981008596	27.691278064583397
51	16.535037839751503	21.869703222865347	28.021784833546313	33.57347410383684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6969.0
1	6986.0
2	7003.0
3	10885.5
4	14768.0
5	9781.5
6	4795.0
7	4835.0
8	4875.0
9	5364.0
10	5853.0
11	6217.0
12	6581.0
13	6763.0
14	6945.0
15	6485.0
16	6025.0
17	5219.0
18	4413.0
19	4319.0
20	4225.0
21	3997.0
22	3769.0
23	3433.0
24	3097.0
25	3058.0
26	3383.5
27	3748.0
28	4047.5
29	4347.0
30	4598.5
31	4850.0
32	5046.5
33	5243.0
34	5938.0
35	6633.0
36	7037.0
37	7441.0
38	8229.0
39	9017.0
40	10429.5
41	11842.0
42	15945.0
43	20048.0
44	24107.5
45	28167.0
46	36941.5
47	45716.0
48	54542.5
49	63369.0
50	59890.5
51	56412.0
52	43364.0
53	30316.0
54	22458.0
55	14600.0
56	10844.0
57	7088.0
58	5578.0
59	4068.0
60	3372.0
61	2676.0
62	2277.5
63	1879.0
64	1490.5
65	1102.0
66	885.0
67	668.0
68	498.0
69	328.0
70	263.0
71	198.0
72	169.0
73	140.0
74	114.0
75	63.0
76	38.0
77	32.0
78	26.0
79	17.0
80	8.0
81	5.5
82	3.0
83	1.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	412397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.952823452774815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46295199504661	30.81978255367862
2	11.281169779396613	9.465538481916095
3	4.842371621864972	6.09453485234484
4	2.5920722816704957	4.349790032390141
5	1.6858767177771197	3.5363644152023483
6	1.2483603655929445	3.1423345213897336
7	0.8913443121989576	2.6176087395722503
8	0.6904499092462107	2.3173058516472542
9	0.5622184445064196	2.122798602984434
>10	2.5595475582409977	17.260769853294818
>50	0.10877861366021642	3.1370863275115632
>100	0.06199211316119859	4.825852571236113
>500	0.007017975074852671	1.7918145979834932
>1k	0.004678650049901781	4.78929494079663
>5k	0.0011696625124754452	3.7291236580516385
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8951	2.17048135655691	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6248	1.5150449688043317	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3715	0.9008309953758151	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	3574	0.8666406399658582	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3245	0.7868631440092919	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	2515	0.609849247205969	Illumina Single End Adapter 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2458	0.5960276141679014	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1662	0.40300972121523676	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	1194	0.2895268394289968	No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1157	0.2805549021937599	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	772	0.18719825798926762	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.17240668579063378	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	655	0.15882753754270762	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.158100083172283	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	637	0.15446281132015996	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	614	0.14888566114690455	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	590	0.14306602618350764	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.14039869349195072	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	569	0.13797384559053533	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	513	0.12439469734260918	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGT	510	0.12366724297218457	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	501	0.12148487986091072	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	413	0.10014621832845534	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8496958028307677E-4	0.0	0.0	0.6505866919497475	0.0
2	4.8496958028307677E-4	0.0	0.0	3.196434503645759	0.0
3	4.8496958028307677E-4	0.0	0.0	4.289798422393956	0.0
4	4.8496958028307677E-4	0.0	0.0	5.742767284922054	0.0
5	4.8496958028307677E-4	0.0	0.0	9.391920891762064	0.0
6	4.8496958028307677E-4	0.0	0.0	11.289606859409744	0.0
7	4.8496958028307677E-4	0.0	0.0	13.253733659556204	0.0
8	4.8496958028307677E-4	0.0	0.0	16.24793584822392	0.0
9	4.8496958028307677E-4	0.0	0.0	17.222967189383045	0.0
10	4.8496958028307677E-4	0.0	0.0	20.259846701115674	0.0
11	4.8496958028307677E-4	0.0	0.0	25.312502273294907	0.0
12	4.8496958028307677E-4	0.0	0.0	28.80088846427108	0.0
13	4.8496958028307677E-4	0.0	0.0	30.031741259029527	0.0
14	4.8496958028307677E-4	0.0	0.0	30.499979388792838	0.0
15	4.8496958028307677E-4	0.0	0.0	31.43839552664059	0.0
16	4.8496958028307677E-4	0.0	0.0	33.46484091785343	0.0
17	4.8496958028307677E-4	0.0	0.0	36.03930193478614	0.0
18	4.8496958028307677E-4	0.0	0.0	38.76337606723618	0.0
19	4.8496958028307677E-4	0.0	0.0	40.49399001447634	0.0
20	4.8496958028307677E-4	0.0	0.0	42.06504896980337	0.0
21	4.8496958028307677E-4	0.0	0.0	43.93921391280732	0.0
22	4.8496958028307677E-4	0.0	0.0	45.85702611803675	0.0
23	4.8496958028307677E-4	0.0	0.0	47.65141356508413	0.0
24	4.8496958028307677E-4	0.0	0.0	48.80515619657757	0.0
25	4.8496958028307677E-4	0.0	0.0	49.68925574143362	0.0
26	4.8496958028307677E-4	0.0	0.0	50.46714694820767	0.0
27	4.8496958028307677E-4	0.0	0.0	51.1182186097377	0.0
28	4.8496958028307677E-4	0.0	0.0	51.74528427704372	0.0
29	4.8496958028307677E-4	0.0	0.0	52.421816841538615	0.0
30	4.8496958028307677E-4	0.0	0.0	53.02487651462062	0.0
31	4.8496958028307677E-4	0.0	0.0	53.566830020586956	0.0
32	4.8496958028307677E-4	0.0	0.0	54.00136276452059	0.0
33	4.8496958028307677E-4	0.0	0.0	54.43662296282466	0.0
34	4.8496958028307677E-4	0.0	0.0	54.8367228665582	0.0
35	4.8496958028307677E-4	0.0	0.0	55.25694900787348	0.0
36	4.8496958028307677E-4	0.0	0.0	55.64419721772952	0.0
37	4.8496958028307677E-4	0.0	0.0	56.02950554926442	0.0
38	4.8496958028307677E-4	0.0	0.0	56.34594820039913	0.0
39	4.8496958028307677E-4	0.0	0.0	56.68639684575785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.028018E-4	45.000004	1
TCTGATT	20	7.028018E-4	45.000004	43
GGAGCGA	20	7.028018E-4	45.000004	9
CATATGG	40	6.7975634E-9	45.000004	2
GGTAATC	20	7.028018E-4	45.000004	8
GTACACG	20	7.028018E-4	45.000004	1
CCGATGA	20	7.028018E-4	45.000004	18
GGATCCG	20	7.028018E-4	45.000004	8
GTCTACG	20	7.028018E-4	45.000004	1
GAGGTGC	20	7.028018E-4	45.000004	9
TACGAAT	20	7.028018E-4	45.000004	12
CTGCAAC	20	7.028018E-4	45.000004	21
CGAATAT	20	7.028018E-4	45.000004	14
TCGTAAG	20	7.028018E-4	45.000004	1
CCTGGAC	20	7.028018E-4	45.000004	11
TAGGCAT	20	7.028018E-4	45.000004	24
GCTGTTG	20	7.028018E-4	45.000004	1
GGCGAAG	20	7.028018E-4	45.000004	1
TGTACGG	20	7.028018E-4	45.000004	2
CTAATGT	20	7.028018E-4	45.000004	16
>>END_MODULE