FastQCFastQC Report
Sat 14 Jan 2017
SRR2935191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335443
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84132.5080266990218902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20350.6066604460370316No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC17140.5109660955810674No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCC15950.47549061986686264No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC15510.4623736372498457No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT11700.3487924923161312No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.24683776379295438No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.22656606338483737No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.19675473925525347No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500.1937736068422951No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC6490.19347549360099928No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.16992454753862804No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC5200.15501888547383608No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG5030.14995096037180683No Hit
ACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC4990.14875850740662347No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG4920.1466717147175526No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC4710.14041133665034No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.11894718327703961No Hit
CGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTG3370.10046416231669762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATG351.208482E-745.0000041
CGTAAGG351.208482E-745.0000042
TGCTACG351.208482E-745.0000041
TCACGAC453.8380676E-1045.025
TCGTTCA207.026135E-445.016
CTCGTTC207.026135E-445.015
CGACGGT453.8380676E-1045.028
CAACGAG253.8848797E-545.014
GCGCGAC207.026135E-445.09
CGACAAG207.026135E-445.012
TAGCCAG207.026135E-445.01
ATCCGGG253.8848797E-545.03
CCCCGAT207.026135E-445.040
CTACGAA253.8848797E-545.011
CTCATGG253.8848797E-545.02
TTGCGAG207.026135E-445.01
TTAACGG207.026135E-445.02
CGGTCTA453.8380676E-1045.031
GCTACGG406.7902874E-945.02
GCTACGA253.8848797E-545.010
Produced by FastQC (version 0.11.5)