##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335443
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76636865279645	31.0	31.0	34.0	30.0	34.0
2	31.876986552111685	33.0	31.0	34.0	30.0	34.0
3	31.899750479217037	33.0	31.0	34.0	30.0	34.0
4	35.66104226351422	37.0	35.0	37.0	33.0	37.0
5	33.04008430642464	37.0	35.0	37.0	28.0	37.0
6	34.12047650420489	37.0	35.0	37.0	28.0	37.0
7	35.43202272815352	37.0	35.0	37.0	32.0	37.0
8	35.402250158745304	37.0	35.0	37.0	33.0	37.0
9	37.461064920120556	39.0	37.0	39.0	35.0	39.0
10	36.95472852317681	39.0	37.0	39.0	33.0	39.0
11	36.81394752610727	39.0	37.0	39.0	33.0	39.0
12	37.09153567073989	39.0	37.0	39.0	33.0	39.0
13	37.03552913609764	39.0	37.0	39.0	33.0	39.0
14	37.94232105007408	40.0	37.0	41.0	33.0	41.0
15	38.10813759714765	40.0	37.0	41.0	33.0	41.0
16	38.1140611072522	40.0	37.0	41.0	33.0	41.0
17	38.081146424280725	40.0	37.0	41.0	33.0	41.0
18	37.97970445053258	39.0	37.0	41.0	33.0	41.0
19	37.77722593704445	39.0	37.0	41.0	33.0	41.0
20	37.576735838875756	39.0	35.0	41.0	33.0	41.0
21	37.510220812477826	39.0	35.0	41.0	33.0	41.0
22	37.50926386897327	39.0	35.0	41.0	33.0	41.0
23	37.2985633922902	39.0	35.0	40.0	33.0	41.0
24	37.06967204562325	39.0	35.0	40.0	32.0	41.0
25	36.855212360967435	39.0	35.0	40.0	32.0	41.0
26	37.09001529320928	39.0	35.0	40.0	33.0	41.0
27	36.97456497825264	39.0	35.0	40.0	32.0	41.0
28	36.994794942806976	39.0	35.0	40.0	32.0	41.0
29	37.04582894858441	39.0	35.0	40.0	33.0	41.0
30	36.835271566257155	39.0	35.0	40.0	32.0	41.0
31	36.603852219304024	39.0	35.0	40.0	31.0	41.0
32	36.33563973611016	39.0	35.0	40.0	30.0	41.0
33	36.22170085528689	39.0	35.0	40.0	30.0	41.0
34	36.003559472101074	39.0	35.0	40.0	30.0	41.0
35	35.7874542023533	39.0	35.0	40.0	27.0	41.0
36	35.628419731519216	39.0	35.0	40.0	26.0	41.0
37	35.46584367537853	39.0	35.0	40.0	25.0	41.0
38	35.392829780320355	38.0	35.0	40.0	25.0	41.0
39	35.37861872210778	39.0	35.0	40.0	25.0	41.0
40	35.26727342648378	38.0	35.0	40.0	24.0	41.0
41	35.14067367630268	38.0	35.0	40.0	23.0	41.0
42	35.17388945364786	38.0	35.0	40.0	23.0	41.0
43	35.063456384542235	38.0	35.0	40.0	23.0	41.0
44	34.98913675348718	38.0	35.0	40.0	23.0	41.0
45	35.00920871802363	38.0	35.0	40.0	23.0	41.0
46	34.93526769078502	38.0	35.0	40.0	23.0	41.0
47	34.852919870141875	38.0	35.0	40.0	23.0	41.0
48	34.81104092200463	38.0	34.0	40.0	23.0	41.0
49	34.82183560247196	38.0	34.0	40.0	23.0	41.0
50	34.73758283821692	38.0	34.0	40.0	22.0	41.0
51	33.762663105207146	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	9.0
12	16.0
13	13.0
14	23.0
15	45.0
16	52.0
17	79.0
18	141.0
19	315.0
20	489.0
21	822.0
22	1268.0
23	1763.0
24	2779.0
25	4030.0
26	5129.0
27	5946.0
28	5896.0
29	5933.0
30	6529.0
31	7684.0
32	9931.0
33	13277.0
34	19614.0
35	27196.0
36	34722.0
37	46800.0
38	69387.0
39	65517.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.54749092990463	22.815798809335714	23.11599884332062	17.520711417439028
2	36.94487588055198	23.173832812132016	24.66141788619825	15.219873421117747
3	27.626154070885367	23.640976261242596	34.978520940964636	13.754348726907404
4	24.94879905080744	24.607459389523704	35.01101528426588	15.432726275402974
5	19.561892780591634	32.63475463789675	32.049856458474316	15.753496123037298
6	21.93636474751299	32.232599875388665	33.238732064762125	12.592303312336222
7	67.70837370283476	7.046800797751033	20.35815324809282	4.886672251321387
8	66.33287920749575	6.808310204714363	20.332813622582673	6.525996965207204
9	59.93298414335669	9.68838222887346	22.656606338483737	7.722027289286109
10	25.886961421165445	33.57202266853086	28.531225871459533	12.009790038844155
11	21.698172267717617	23.57121776277937	36.02847577680858	18.702134192694437
12	21.531228852591948	20.61095327671169	40.15764228199724	17.700175588699125
13	21.51811186997493	21.602477917261652	41.61899339082944	15.260416821933982
14	16.25373014193171	26.130519939304143	39.23378934722144	18.381960571542706
15	13.913243084518085	25.74446329182603	42.38633687392493	17.95595674973095
16	16.378639590034673	26.812006808906432	37.2233136479223	19.5860399531366
17	15.92371878381722	26.222935044105856	36.699230569724214	21.15411560235271
18	15.847103680804189	25.502395339893813	40.545189495681825	18.105311483620167
19	15.132526241418065	26.24887089609859	38.83878930250445	19.779813559978894
20	14.98466207373533	28.969750449405712	39.28208369231136	16.7635037845476
21	15.562405535366663	26.915153990394792	40.686495172056055	16.835945302182488
22	14.20658651395319	25.039425476161377	38.26372886004478	22.490259149840657
23	14.006552529043685	25.336644377733325	39.30921199726928	21.347591095953707
24	15.90016783775485	25.31219909194707	37.197675909170854	21.589957161127227
25	13.76448457711146	28.420923972180073	35.391407780159376	22.423183670549097
26	13.630035505287038	28.964384411062387	36.30244184555945	21.10313823809112
27	16.62517924058633	27.356957813995226	37.017317398186876	19.00054554723157
28	12.394952346598378	28.270973011808266	36.657196602701504	22.676878038891854
29	15.2732356913097	28.831127792203148	35.1961436071106	20.699492909376556
30	20.570707989136753	28.235795649335355	32.86668673962491	18.326809621902974
31	15.898975384789665	33.2974603732974	30.2957581466896	20.507806095223334
32	17.22617553503874	34.141418959405925	28.992407055744195	19.639998449811145
33	21.47458733674574	28.58250134896242	28.93248629424373	21.010425020048114
34	16.189933908294403	29.442259936859617	30.641867619834066	23.72593853501191
35	17.72760200689834	32.80974711053741	27.843478623790034	21.61917225877422
36	23.23673470604544	31.418750726651023	26.89428606350408	18.450228503799455
37	18.594813425827937	33.30849056322535	29.579690141096997	18.51700586984972
38	18.932873841457415	34.14827556395573	27.201640815280093	19.717209779306767
39	22.986915809839527	27.407338951774218	28.493067376573666	21.112677861812585
40	21.46415337330038	27.549538967872333	29.51798070014876	21.468326958678524
41	19.882662628225958	30.635309128525563	26.227108629483997	23.254919613764486
42	21.99568928253086	29.01446743560009	29.140867449909525	19.848975831959528
43	23.059953553957005	27.53552764553143	30.103475106053786	19.301043694457775
44	21.100157105678164	26.763712463816507	28.638248525084737	23.49788190542059
45	21.67134207600099	25.457678353699436	27.985380526646853	24.88559904365272
46	23.14968563958705	27.284814409601633	29.432422199896852	20.133077750914463
47	18.46274925993388	28.678195699418378	32.91736599064521	19.941689050002534
48	19.058975742525554	27.61422954123353	31.808682846265985	21.51811186997493
49	22.395757252349878	23.90927817840885	33.25870565192894	20.43625891731233
50	20.60111553974893	22.969625241844366	31.986954564560893	24.44230465384581
51	19.456658806414204	22.413047820345035	29.288433504350962	28.841859868889795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2740.0
1	3874.0
2	5008.0
3	9349.0
4	13690.0
5	8859.5
6	4029.0
7	3829.0
8	3629.0
9	3700.5
10	3772.0
11	3957.0
12	4142.0
13	3932.5
14	3723.0
15	3588.5
16	3454.0
17	3081.0
18	2708.0
19	2915.0
20	3122.0
21	3082.0
22	3042.0
23	2827.0
24	2612.0
25	3006.5
26	3610.5
27	3820.0
28	4132.5
29	4445.0
30	4730.0
31	5015.0
32	5458.0
33	5901.0
34	6737.5
35	7574.0
36	7786.0
37	7998.0
38	8604.5
39	9211.0
40	10375.5
41	11540.0
42	13809.0
43	16078.0
44	18680.0
45	21282.0
46	27145.5
47	33009.0
48	38078.5
49	43148.0
50	41825.5
51	40503.0
52	32082.5
53	23662.0
54	18604.0
55	13546.0
56	11070.5
57	8595.0
58	7410.5
59	6226.0
60	5345.0
61	4464.0
62	4054.5
63	3645.0
64	3038.0
65	2431.0
66	2038.5
67	1646.0
68	1283.0
69	920.0
70	826.0
71	732.0
72	592.0
73	452.0
74	316.0
75	176.0
76	172.0
77	120.0
78	68.0
79	60.0
80	52.0
81	40.0
82	28.0
83	22.0
84	16.0
85	13.5
86	11.0
87	5.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.50685609697904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65741013870414	32.7825975351947
2	10.948085722768127	9.745297516012636
3	4.436138029602216	5.92315670729526
4	2.5931195261255153	4.616463903661392
5	1.6746942993702738	3.726768909425196
6	1.2854263101613779	3.432617028577392
7	0.9080654303429723	2.8290596204441205
8	0.7927789203493715	2.8227277859766304
9	0.6502759173985642	2.6047563011090036
>10	2.9363645779292855	19.69657763108823
>50	0.07004067113620598	2.1799845031861405
>100	0.038080364889587714	3.097315202777587
>500	0.00544005212708396	1.555618540382724
>1k	0.003400032579427475	2.440868390697796
>5k	6.80006515885495E-4	2.546190424171179
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8413	2.5080266990218902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2035	0.6066604460370316	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1714	0.5109660955810674	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCC	1595	0.47549061986686264	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1551	0.4623736372498457	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	1170	0.3487924923161312	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.24683776379295438	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	760	0.22656606338483737	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	660	0.19675473925525347	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	650	0.1937736068422951	No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	649	0.19347549360099928	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	570	0.16992454753862804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	520	0.15501888547383608	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	503	0.14995096037180683	No Hit
ACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	499	0.14875850740662347	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	492	0.1466717147175526	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	471	0.14041133665034	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.11894718327703961	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTG	337	0.10046416231669762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4197434437445408	0.0
2	0.0	0.0	0.0	2.0182266435728278	0.0
3	0.0	0.0	0.0	2.739958800750053	0.0
4	0.0	0.0	0.0	3.7064419290311617	0.0
5	0.0	0.0	0.0	6.291083731066083	0.0
6	0.0	0.0	0.0	7.614110295937015	0.0
7	0.0	0.0	0.0	9.066518007530341	0.0
8	0.0	0.0	0.0	11.18342013397209	0.0
9	0.0	0.0	0.0	11.914691914870783	0.0
10	0.0	0.0	0.0	14.222386515741869	0.0
11	0.0	0.0	0.0	17.995307697582003	0.0
12	0.0	0.0	0.0	20.925760859520096	0.0
13	0.0	0.0	0.0	22.0430892878969	0.0
14	0.0	0.0	0.0	22.503376132457674	0.0
15	0.0	0.0	0.0	23.388772459106317	0.0
16	0.0	0.0	0.0	25.188482096809295	0.0
17	0.0	0.0	0.0	27.353380455099675	0.0
18	0.0	0.0	0.0	29.45120333409849	0.0
19	0.0	0.0	0.0	30.89615821465942	0.0
20	0.0	0.0	0.0	32.38881121382769	0.0
21	0.0	0.0	0.0	34.04602272219125	0.0
22	0.0	0.0	0.0	35.75957763315973	0.0
23	0.0	0.0	0.0	37.06352495058773	0.0
24	0.0	0.0	0.0	38.010332604943315	0.0
25	0.0	0.0	0.0	38.763366652456604	0.0
26	0.0	0.0	0.0	39.468404468121264	0.0
27	2.9811324129583864E-4	0.0	0.0	40.14124605372597	0.0
28	2.9811324129583864E-4	0.0	0.0	40.71034423135973	0.0
29	2.9811324129583864E-4	0.0	0.0	41.22280089314727	0.0
30	2.9811324129583864E-4	0.0	0.0	41.80948775201748	0.0
31	2.9811324129583864E-4	0.0	0.0	42.29660478829488	0.0
32	2.9811324129583864E-4	0.0	0.0	42.71306898638517	0.0
33	2.9811324129583864E-4	0.0	0.0	43.07974827317905	0.0
34	2.9811324129583864E-4	0.0	0.0	43.453582277764035	0.0
35	2.9811324129583864E-4	0.0	0.0	43.821752130764395	0.0
36	5.962264825916773E-4	0.0	0.0	44.14997480943111	0.0
37	5.962264825916773E-4	0.0	0.0	44.5300691920833	0.0
38	5.962264825916773E-4	0.0	0.0	44.90360508342699	0.0
39	5.962264825916773E-4	0.0	0.0	45.36329570150517	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATG	35	1.208482E-7	45.000004	1
CGTAAGG	35	1.208482E-7	45.000004	2
TGCTACG	35	1.208482E-7	45.000004	1
TCACGAC	45	3.8380676E-10	45.0	25
TCGTTCA	20	7.026135E-4	45.0	16
CTCGTTC	20	7.026135E-4	45.0	15
CGACGGT	45	3.8380676E-10	45.0	28
CAACGAG	25	3.8848797E-5	45.0	14
GCGCGAC	20	7.026135E-4	45.0	9
CGACAAG	20	7.026135E-4	45.0	12
TAGCCAG	20	7.026135E-4	45.0	1
ATCCGGG	25	3.8848797E-5	45.0	3
CCCCGAT	20	7.026135E-4	45.0	40
CTACGAA	25	3.8848797E-5	45.0	11
CTCATGG	25	3.8848797E-5	45.0	2
TTGCGAG	20	7.026135E-4	45.0	1
TTAACGG	20	7.026135E-4	45.0	2
CGGTCTA	45	3.8380676E-10	45.0	31
GCTACGG	40	6.7902874E-9	45.0	2
GCTACGA	25	3.8848797E-5	45.0	10
>>END_MODULE