SRR2935190.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935190.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453062
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9643	2.1284062666919756	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5251	1.159002520626316	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	3397	0.7497870048690908	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2861	0.6314809010687279	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2792	0.6162511974078603	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	2084	0.459981194626784	TruSeq Adapter, Index 14 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1687	0.37235521849106745	No Hit
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1587	0.35028318419995497	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG	1080	0.23837797034401473	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.23219780074250326	No Hit
ACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	994	0.219396020853658	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	832	0.1836393253020558	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	710	0.15671144346689858	No Hit
ACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	668	0.14744118906463133	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	662	0.14611686700716459	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.13331508711831935	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	577	0.12735563785971898	TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	545	0.120292586886563	TruSeq Adapter, Index 14 (95% over 21bp)
TGTTTTTTTTGTTTTTGTTTTTGTTTTTTGTTTTATTTTGATTTGTTTTTT	504	0.11124305282720687	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	40	6.7993824E-9	45.0	2
TTGTGCG	40	6.7993824E-9	45.0	1
CGACGGT	20	7.0287567E-4	45.0	28
TGAAGCG	20	7.0287567E-4	45.0	1
GGCACCG	20	7.0287567E-4	45.0	8
GGATCGC	20	7.0287567E-4	45.0	9
TTCGTAG	40	6.7993824E-9	45.0	1
TACGGGT	35	1.2097553E-7	45.0	4
ACGGGTC	20	7.0287567E-4	45.0	5
GCACCGA	20	7.0287567E-4	45.0	9
TATTGCG	25	3.8870516E-5	45.0	1
CGGGGCG	20	7.0287567E-4	45.0	6
TCTACGG	20	7.0287567E-4	45.0	2
TACGATG	40	6.7993824E-9	45.0	1
CGGGCTC	20	7.0287567E-4	45.0	6
CGGGCAT	20	7.0287567E-4	45.0	6
ACTACGG	20	7.0287567E-4	45.0	2
GACGGTC	20	7.0287567E-4	45.0	29
ATGACGG	20	7.0287567E-4	45.0	2
GCTAGCG	25	3.8870516E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content