##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1542409
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938308191925746	33.0	31.0	34.0	30.0	34.0
2	32.06998143812698	33.0	31.0	34.0	30.0	34.0
3	32.01214723202471	33.0	31.0	34.0	30.0	34.0
4	35.7644489885627	37.0	35.0	37.0	35.0	37.0
5	33.11875838380092	37.0	35.0	37.0	28.0	37.0
6	34.22795574973953	37.0	35.0	37.0	28.0	37.0
7	35.58548024551205	37.0	35.0	37.0	32.0	37.0
8	35.607311679327594	37.0	35.0	37.0	33.0	37.0
9	37.70121089801732	39.0	37.0	39.0	35.0	39.0
10	36.9742344605095	39.0	37.0	39.0	32.0	39.0
11	36.97376506490821	39.0	37.0	39.0	33.0	39.0
12	37.28674819713837	39.0	37.0	39.0	34.0	39.0
13	37.274130272839436	39.0	37.0	39.0	34.0	39.0
14	38.29645703571491	40.0	38.0	41.0	33.0	41.0
15	38.427751653420074	40.0	38.0	41.0	34.0	41.0
16	38.41762139614071	40.0	38.0	41.0	34.0	41.0
17	38.369436381660115	40.0	38.0	41.0	34.0	41.0
18	38.1378512443846	39.0	38.0	41.0	34.0	41.0
19	37.77741442120735	39.0	37.0	41.0	34.0	41.0
20	37.43212533121889	39.0	35.0	41.0	34.0	41.0
21	37.32997992101965	39.0	35.0	40.0	33.0	41.0
22	37.34087910534755	39.0	35.0	40.0	33.0	41.0
23	37.11365662415092	39.0	35.0	40.0	33.0	41.0
24	36.84577112815084	38.0	35.0	40.0	33.0	41.0
25	36.64156912984818	38.0	35.0	40.0	32.0	41.0
26	36.90920825799123	38.0	35.0	40.0	33.0	41.0
27	36.82555210712593	38.0	35.0	40.0	33.0	41.0
28	36.842126828876125	39.0	35.0	40.0	33.0	41.0
29	36.84504369463612	39.0	35.0	40.0	33.0	41.0
30	36.50238166400741	39.0	35.0	40.0	31.0	41.0
31	36.2056698320614	39.0	35.0	40.0	31.0	41.0
32	35.76967587715061	39.0	35.0	40.0	28.0	41.0
33	35.30345453119114	39.0	35.0	41.0	23.0	41.0
34	34.89299206630667	39.0	35.0	41.0	20.0	41.0
35	34.575530874106676	39.0	35.0	41.0	18.0	41.0
36	34.36491034479182	39.0	35.0	41.0	15.0	41.0
37	34.301735791220096	39.0	35.0	41.0	15.0	41.0
38	34.220672337881844	39.0	34.0	41.0	15.0	41.0
39	34.0726279475807	39.0	34.0	40.0	15.0	41.0
40	33.914024101259784	38.0	34.0	40.0	12.0	41.0
41	33.79225873293011	38.0	34.0	40.0	12.0	41.0
42	33.8492604750102	38.0	34.0	40.0	12.0	41.0
43	33.77404307158477	38.0	34.0	40.0	10.0	41.0
44	33.68110079751869	38.0	33.0	40.0	10.0	41.0
45	33.6376564192766	38.0	33.0	40.0	10.0	41.0
46	33.54042734449812	38.0	33.0	40.0	10.0	41.0
47	33.48217885139415	38.0	33.0	40.0	10.0	41.0
48	33.43748383210938	38.0	33.0	40.0	10.0	41.0
49	33.40234075397641	38.0	33.0	40.0	10.0	41.0
50	33.302163693287575	38.0	33.0	40.0	10.0	41.0
51	32.453574246519565	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	22.0
10	31.0
11	30.0
12	37.0
13	59.0
14	102.0
15	149.0
16	253.0
17	492.0
18	891.0
19	1599.0
20	2765.0
21	4555.0
22	7214.0
23	11518.0
24	19332.0
25	31764.0
26	42953.0
27	44828.0
28	39762.0
29	34449.0
30	32430.0
31	34537.0
32	40648.0
33	53151.0
34	77878.0
35	107978.0
36	141249.0
37	179996.0
38	299063.0
39	332577.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.069969119734132	19.127157582716386	21.27833797650299	28.52453532104649
2	44.48262425854621	19.878968548549704	22.024119413203632	13.614287779700456
3	24.602683205297687	20.67155987808681	42.50480903573566	12.220947880879844
4	22.469072729736407	21.74228755148602	41.64355887446196	14.145080844315613
5	17.94874122233467	30.518429288210847	37.96483293341779	13.567996556036693
6	20.707607385589686	28.291782529795924	39.533483012612095	11.467127072002302
7	65.17130021933222	5.078289869937222	26.179437490315472	3.5709724204150777
8	65.59563643625005	4.298016933251816	26.101377779823636	4.004968850674497
9	59.87497479591989	7.595845200592061	27.990954409628056	4.538225593859995
10	22.263290735466406	32.03488828190188	35.65176292410119	10.05005805853052
11	20.73302217505214	21.843881875689263	41.95994706981093	15.463148879447669
12	20.140507478885304	19.528737189681856	44.071578939178906	16.259176392253934
13	20.336305091580765	19.51246394438829	45.682111554068996	14.46911940996195
14	16.10046362540675	22.29123403714579	45.099127403950575	16.509174933496887
15	14.563582033040523	23.469909732113855	46.135622911951366	15.830885322894254
16	17.35512435417584	23.796022974451006	43.55478994222673	15.294062729146422
17	16.843781383537053	23.274695622237683	42.691789272495164	17.1897337217301
18	16.903363504751333	22.878886209818535	44.84251583075566	15.375234454674475
19	16.00308348823172	24.321823848278893	43.66241379556266	16.01267886792673
20	16.132491446821174	25.75354526587954	43.25519366134404	14.858769625955242
21	16.093461591575256	24.202594772203742	45.18898683812141	14.514956798099595
22	15.820511939440188	21.637970214126085	44.31729845974706	18.22421938668667
23	13.946300883877106	23.034681462569267	45.00369227617318	18.01532537738045
24	15.19421891340105	23.102108455020684	43.5442868914795	18.15938574009877
25	15.152271544058676	25.01269118631958	42.053891023716794	17.781146245904942
26	14.420494175021023	24.713743241902762	42.84985370287647	18.015908880199742
27	15.005228833597315	23.558926328879046	43.42142713119542	18.014417706328217
28	12.918428250872498	23.385950159782524	43.626884957232484	20.068736632112493
29	14.78622077542338	22.61455943267966	43.99760374842211	18.60161604347485
30	16.817718257608718	22.97464550582887	41.89984627942394	18.307789957138475
31	15.107795662499376	25.353586500078773	40.05280052178119	19.485817315640663
32	15.66802320266544	24.449027462884356	40.36082517672031	19.522124157729888
33	16.792238634499668	23.89372727985897	38.45692031101997	20.85711377462139
34	15.405835935863962	24.496161523953763	39.1679509131495	20.93005162703278
35	15.351181171790362	24.892619272838786	37.925738244525284	21.830461310845568
36	17.18876121703128	25.315140147652148	36.47320522636992	21.022893408946654
37	15.894098128317458	25.98279704021437	36.61091189172262	21.512192939745553
38	17.26228257226196	25.422764000988064	35.978719003843985	21.336234422905985
39	19.461310197230436	24.141845645350877	36.21840899527946	20.178435162139223
40	19.4856228147009	24.843540202371745	36.04899867674528	19.62183830618208
41	16.82588729707879	25.261393054630776	35.26943891017233	22.6432807381181
42	17.263125409667605	23.901636984742698	37.340031081250174	21.495206524339526
43	17.88111972894349	23.125254066852566	37.48175743269133	21.511868771512617
44	18.947114546141783	23.27197260908099	35.302957905458285	22.47795493931895
45	19.023553415468918	22.467257387631946	35.45531697493985	23.053872221959285
46	19.043133176738465	23.50783741536778	35.94267149634111	21.50635791155264
47	16.136770467495975	24.743437052040022	37.83140528873989	21.288387191724116
48	16.422751682595212	23.64878576304988	37.70653568541159	22.221926868943324
49	18.78217774922216	21.92271958994015	38.03200059128285	21.263102069554833
50	17.550532964991774	22.092518910353867	37.32038648633404	23.036561638320315
51	17.215602346718672	21.79324679770411	35.58524360270201	25.40590725287521
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5312.0
1	7778.0
2	10244.0
3	59903.0
4	109562.0
5	68378.5
6	27195.0
7	25826.5
8	24458.0
9	24814.5
10	25171.0
11	25010.5
12	24850.0
13	24130.5
14	23411.0
15	22243.5
16	21076.0
17	20569.5
18	20063.0
19	19541.0
20	19019.0
21	18059.0
22	17099.0
23	17383.5
24	17668.0
25	17833.0
26	20079.5
27	22161.0
28	23180.5
29	24200.0
30	26850.0
31	29500.0
32	32118.5
33	34737.0
34	37167.5
35	39598.0
36	41754.5
37	43911.0
38	46984.5
39	50058.0
40	54289.0
41	58520.0
42	65604.5
43	72689.0
44	81559.5
45	90430.0
46	106016.0
47	121602.0
48	136116.5
49	150631.0
50	143513.0
51	136395.0
52	114451.0
53	92507.0
54	77211.0
55	61915.0
56	52146.5
57	42378.0
58	38227.0
59	34076.0
60	29763.0
61	25450.0
62	21753.5
63	18057.0
64	15191.0
65	12325.0
66	10026.0
67	7727.0
68	6152.5
69	4578.0
70	3551.0
71	2524.0
72	2043.0
73	1562.0
74	1155.5
75	651.0
76	553.0
77	383.5
78	214.0
79	161.0
80	108.0
81	89.5
82	71.0
83	53.0
84	35.0
85	22.0
86	9.0
87	8.0
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1542409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.73610912710351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76702591787233	26.361464881829583
2	9.880152936061863	7.061564470311578
3	4.219236138212953	4.5233724920459055
4	2.325570050687367	3.324273004563496
5	1.511640144709385	2.701006858612256
6	1.1035460671748512	2.366186561000783
7	0.8576518962472379	2.1454399230150987
8	0.7028678761894916	2.009421050035452
9	0.5902745658442716	1.8984704669968082
>10	4.797529985126064	31.543627767427417
>50	0.17863726171469813	4.31230003975206
>100	0.05986218461580059	3.6814383073986074
>500	0.0024395213146398862	0.6152154963434635
>1k	0.0031901432576060054	2.12716759799593
>5k	1.876554857415297E-4	0.43247490036178293
>10k+	1.876554857415297E-4	4.896576182309862
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73017	4.733958372908872	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	6449	0.41811218684538276	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3652	0.23677247733901968	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	3257	0.21116318693679823	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2939	0.19054608732184525	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	2485	0.16111161177093752	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	2402	0.15573041910414165	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1819	0.11793240314339451	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGT	1786	0.11579289280599374	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTC	1706	0.11060620107896155	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2966729317580487E-4	0.0	0.0	0.17206849804429306	0.0
2	1.2966729317580487E-4	0.0	0.0	0.7762532505969558	0.0
3	1.2966729317580487E-4	0.0	0.0	1.1279757833363264	0.0
4	1.2966729317580487E-4	0.0	0.0	1.6144226336853584	0.0
5	1.2966729317580487E-4	0.0	0.0	2.95706262087423	0.0
6	1.2966729317580487E-4	0.0	0.0	3.9787760574529845	0.0
7	1.2966729317580487E-4	0.0	0.0	4.915168415122059	0.0
8	1.2966729317580487E-4	0.0	0.0	6.330746254722321	0.0
9	1.2966729317580487E-4	0.0	0.0	6.911850229089691	0.0
10	1.2966729317580487E-4	0.0	0.0	8.399587917342288	0.0
11	1.945009397637073E-4	0.0	0.0	10.698653858995895	0.0
12	1.945009397637073E-4	0.0	0.0	12.317031345123116	0.0
13	1.945009397637073E-4	0.0	0.0	12.982030058175232	0.0
14	1.945009397637073E-4	0.0	0.0	13.261138906736152	0.0
15	1.945009397637073E-4	0.0	0.0	13.75199444505316	0.0
16	2.5933458635160974E-4	0.0	0.0	14.758796142916697	0.0
17	2.5933458635160974E-4	0.0	0.0	16.047818704377374	0.0
18	2.5933458635160974E-4	0.0	0.0	17.286011686913135	0.0
19	3.2416823293951214E-4	0.0	0.0	18.07004497510064	0.0
20	3.890018795274146E-4	0.0	0.0	18.991525594054494	0.0
21	3.890018795274146E-4	0.0	0.0	20.02011139717157	0.0
22	3.890018795274146E-4	0.0	0.0	21.129220589350815	0.0
23	3.890018795274146E-4	0.0	0.0	22.06593711525283	0.0
24	3.890018795274146E-4	0.0	0.0	22.73132483018447	0.0
25	3.890018795274146E-4	0.0	0.0	23.276381297048967	0.0
26	4.5383552611531704E-4	0.0	0.0	23.75193609477123	0.0
27	5.186691727032195E-4	0.0	0.0	24.223082204525518	0.0
28	5.186691727032195E-4	0.0	0.0	24.686513110335845	0.0
29	5.186691727032195E-4	0.0	0.0	25.137106954121766	0.0
30	5.186691727032195E-4	0.0	0.0	25.579077922911498	0.0
31	5.835028192911219E-4	0.0	0.0	25.981176199049667	0.0
32	5.835028192911219E-4	0.0	0.0	26.340095266560297	0.0
33	5.835028192911219E-4	0.0	0.0	26.678008232576445	0.0
34	5.835028192911219E-4	0.0	0.0	27.025451744641014	0.0
35	5.835028192911219E-4	0.0	0.0	27.367125062159257	0.0
36	5.835028192911219E-4	0.0	0.0	27.680855077998118	0.0
37	5.835028192911219E-4	0.0	0.0	28.001522294021886	0.0
38	6.483364658790243E-4	0.0	0.0	28.352596490295376	0.0
39	6.483364658790243E-4	0.0	0.0	28.849287056805295	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAA	20	7.0340384E-4	45.0	9
TCGTAAT	20	7.0340384E-4	45.0	28
CGCGTCA	35	1.2122837E-7	45.0	39
CGTTTTT	27740	0.0	44.12401	1
CGTTATT	2100	0.0	43.071426	1
TCGTTGG	85	0.0	42.35294	2
CGGTCTA	315	0.0	42.142857	31
TACGGGA	940	0.0	40.45213	4
CGACGGT	335	0.0	40.298508	28
CGCCGCA	90	0.0	40.0	18
TCACGAC	355	0.0	39.929577	25
TAGTTCG	40	3.459245E-7	39.375	1
ACGGTCT	340	0.0	39.044117	30
GTTTGCG	35	6.24968E-6	38.571426	1
TTTAGCG	70	0.0	38.571426	1
CACGACG	350	0.0	38.571426	26
TATAGCG	70	0.0	38.571426	1
AGATGCG	35	6.24968E-6	38.571426	1
CTCACGA	370	0.0	38.31081	24
TAAGCGG	130	0.0	38.07692	2
>>END_MODULE