FastQCFastQC Report
Sat 14 Jan 2017
SRR2935186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389982
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227991.6402370678181444No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC108120.777851799519706TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC105020.7555493524376575TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT78930.5678490800600295TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC61010.43892654725025215TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC55810.4015159908545578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31520.22676552646005488No Hit
ACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC30520.2195711886916521TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG25050.18021816109848904TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG23440.1686352772913606TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT20200.14532562292173568No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC19500.14028958648385376No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC19370.1393543225739614No Hit
GCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG19210.13820322853101694No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG17930.12899447618746143No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC17120.12316706259505518No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT15800.11367053674076355TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG15280.10992948110119412No Hit
GACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT14930.10741146288225314No Hit
AGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG14360.10331069035426359TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC14180.10201570955595109No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG207.0337974E-445.025
TTAGACG253.8912287E-545.01
TAACGCG253.8912287E-545.01
TCGCTAG207.0337974E-445.01
ACTAACG253.8912287E-545.01
ATAACGT351.2121745E-745.031
TAGGACG253.8912287E-545.01
TTACCGG207.0337974E-445.02
CGATAGA207.0337974E-445.012
CGCCCAA207.0337974E-445.037
TAGTACG453.8562575E-1045.01
AGTCACG302.1658325E-644.9999961
TGTATCG302.1658325E-644.9999961
TAACGAG302.1658325E-644.9999961
GTTCGCG302.1658325E-644.9999961
CGTTATT6650.043.6466141
CGTTTTT91250.043.5698661
CTACGAA800.042.187511
ACGTAAG750.042.01
AGTACGG1350.041.6666682
Produced by FastQC (version 0.11.5)