##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935186.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1389982
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52331109323718	31.0	31.0	34.0	30.0	34.0
2	31.626189403891562	31.0	31.0	34.0	30.0	34.0
3	31.66028337057602	31.0	31.0	34.0	30.0	34.0
4	35.49741579387359	37.0	35.0	37.0	33.0	37.0
5	33.02975506157634	37.0	35.0	37.0	28.0	37.0
6	33.99384236630402	36.0	35.0	37.0	28.0	37.0
7	35.351474335638876	37.0	35.0	37.0	32.0	37.0
8	35.35734419582412	37.0	35.0	37.0	33.0	37.0
9	37.33072946268369	39.0	37.0	39.0	34.0	39.0
10	36.71295959228249	39.0	35.0	39.0	32.0	39.0
11	36.64751557933844	39.0	37.0	39.0	32.0	39.0
12	37.042387599263876	39.0	37.0	39.0	33.0	39.0
13	37.033629212464625	39.0	37.0	39.0	33.0	39.0
14	37.87670631706022	39.0	37.0	41.0	33.0	41.0
15	37.99934891243196	39.0	37.0	41.0	33.0	41.0
16	37.96282110128045	39.0	37.0	41.0	33.0	41.0
17	37.981005509423866	39.0	37.0	41.0	33.0	41.0
18	37.94604606390586	39.0	37.0	40.0	33.0	41.0
19	37.811886772634466	39.0	37.0	40.0	33.0	41.0
20	37.66992450261946	39.0	36.0	40.0	33.0	41.0
21	37.59262781820196	39.0	36.0	40.0	33.0	41.0
22	37.60700714109967	39.0	36.0	40.0	33.0	41.0
23	37.337215877615684	39.0	35.0	40.0	32.0	41.0
24	37.07364555800003	39.0	35.0	40.0	32.0	41.0
25	36.8688508196509	39.0	35.0	40.0	31.0	41.0
26	37.22990513546218	39.0	35.0	40.0	32.0	41.0
27	37.185841255498275	39.0	35.0	40.0	33.0	41.0
28	37.31589977424168	39.0	36.0	40.0	33.0	41.0
29	37.486203418461535	39.0	36.0	40.0	33.0	41.0
30	37.26547178308784	39.0	36.0	40.0	33.0	41.0
31	37.15602576148468	39.0	36.0	40.0	32.0	41.0
32	37.02328663248877	39.0	36.0	40.0	32.0	41.0
33	36.87335951113036	39.0	36.0	40.0	31.0	41.0
34	36.831161122949794	39.0	36.0	41.0	31.0	41.0
35	36.680631116086396	39.0	36.0	41.0	31.0	41.0
36	36.51940600669649	39.0	35.0	41.0	30.0	41.0
37	36.528365115519485	39.0	35.0	41.0	30.0	41.0
38	36.424329955351936	39.0	35.0	40.0	30.0	41.0
39	36.3358856445623	39.0	35.0	40.0	30.0	41.0
40	36.27823597715654	39.0	35.0	40.0	30.0	41.0
41	36.1005977055818	39.0	35.0	40.0	29.0	41.0
42	36.1334261882528	39.0	35.0	40.0	29.0	41.0
43	36.030089598282565	39.0	35.0	40.0	29.0	41.0
44	36.0155505610864	39.0	35.0	40.0	29.0	41.0
45	36.064437525090256	39.0	35.0	40.0	29.0	41.0
46	35.91725072698783	39.0	35.0	40.0	28.0	41.0
47	35.78172379210666	39.0	35.0	40.0	27.0	41.0
48	35.74092182488694	39.0	35.0	40.0	27.0	41.0
49	35.76168684198788	39.0	35.0	40.0	27.0	41.0
50	35.690316853023994	39.0	35.0	40.0	27.0	41.0
51	34.66930938674026	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	12.0
10	23.0
11	33.0
12	32.0
13	44.0
14	46.0
15	82.0
16	136.0
17	220.0
18	433.0
19	798.0
20	1294.0
21	2243.0
22	3639.0
23	5571.0
24	8644.0
25	13167.0
26	17631.0
27	18852.0
28	19152.0
29	19835.0
30	22873.0
31	28010.0
32	36644.0
33	50379.0
34	74061.0
35	103622.0
36	147868.0
37	214778.0
38	339992.0
39	259825.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.67082163653918	22.588206178209504	24.25851557789957	17.48245660735175
2	33.73727141790325	24.312473111162593	25.378170364796095	16.572085106138065
3	26.451925276730204	23.879230090749378	34.578649219917956	15.090195412602464
4	24.70111123741171	24.681542638681652	33.33079133398849	17.286554789918142
5	18.294985114915157	31.231555516546255	32.00782456175691	18.46563480678167
6	21.08948173429584	31.29249155744463	33.037190409659985	14.58083629859955
7	69.05190139152882	5.302586652201251	19.02564205867414	6.619869897595796
8	65.642216949572	4.4390502898598685	20.168678443317972	9.75005431725015
9	59.866170928832176	7.226712288360569	23.10490351673619	9.802213266071073
10	25.0524826940205	32.74193478764473	28.27230856226915	13.933273956065618
11	16.09567605911443	20.587676674949748	39.78166623740451	23.534981028531305
12	17.096624272832308	18.113400029640673	43.18868877438701	21.601286923140012
13	18.897510903018887	20.38458051974774	45.390012244762886	15.327896332470491
14	15.213002758309099	24.734421021279413	39.38137328397058	20.671202936440903
15	11.239498065442573	24.818450886414357	44.38568269229386	19.556368355849212
16	12.042314217018637	26.974737802360032	37.96250598928619	23.02044199133514
17	11.922096832908629	26.346024624779314	36.25269967524759	25.479178867064466
18	12.16915039187558	24.65952796511034	42.39306696057935	20.778254682434735
19	11.924902624638305	26.852290173541814	38.69942200690369	22.523385194916195
20	12.521241282261208	29.390956141878092	38.72611300002446	19.361689575836234
21	13.000024460748413	28.493318618514486	39.875336515149115	18.631320405587985
22	10.877119272048127	25.489898430339387	35.760103368245055	27.872878929367428
23	10.735606648143644	25.902925361623385	37.21616538919209	26.145302601040875
24	15.497538817049431	23.341885002827375	35.68988663162544	25.47068954849775
25	11.881089107628732	28.183098773940955	33.173954770637316	26.761857347792994
26	11.787131056373392	26.651712036558745	38.29279803623356	23.268358870834298
27	15.475092483212013	24.364128456339724	37.93171422363743	22.229064836810835
28	9.872789719579103	23.332964023994553	37.20472639214033	29.58951986428601
29	12.655919285285707	19.480827809280985	38.31502853993793	29.54822436549538
30	16.200857277288485	22.448204365236386	35.84981676021704	25.50112159725809
31	12.098501994989864	25.66860578050651	30.50255327047401	31.73033895402962
32	11.732885749599635	24.565713800610368	32.54826321491933	31.153137234870666
33	12.767647350829003	22.392807964419685	30.712915706822102	34.126628977929215
34	11.616121647618458	18.87218683407411	32.29962690164333	37.2120646166641
35	10.429775349608844	19.35003474865142	37.574947013702335	32.6452428880374
36	12.225194283091435	23.58901050517201	35.844277120135374	28.341518091601188
37	11.174317365260846	21.9179816717051	35.245852104559624	31.66184885847443
38	11.77029630599533	22.82705819212047	29.418654342286448	35.98399115959775
39	16.259706960234016	20.600554539555187	28.0545359580196	35.0852025421912
40	16.06646704777472	17.562961246980176	35.032252216215745	31.338319489029352
41	13.608521549199917	23.000873392605083	33.71496897082121	29.675636087373793
42	15.707901253397527	22.868137860778052	37.73336633136257	23.690594554461857
43	17.55555107907872	20.927896908017512	34.94937344512375	26.567178567780015
44	16.47086077373664	18.598586168741754	29.789450510869926	35.14110254665168
45	18.627507406570732	14.141118374194775	30.58284207996938	36.648532139265114
46	20.927177474240676	16.940938803524077	33.18042967462888	28.951454047606372
47	12.530953638248555	19.973352172905837	39.409934804911146	28.085759383934466
48	13.875575367163028	19.266796260671	36.17938937338757	30.6782389987784
49	18.839380653850192	15.619410898846173	37.61041509890056	27.93079334840307
50	17.1507256928507	13.492117164107162	35.509956244037696	33.84720089900445
51	16.106899226033143	13.619241112474839	30.21449198622716	40.05936767526486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4489.0
1	5978.5
2	7468.0
3	21477.0
4	35486.0
5	22542.0
6	9598.0
7	9455.5
8	9313.0
9	9711.0
10	10109.0
11	10325.5
12	10542.0
13	10770.5
14	10999.0
15	10503.5
16	10008.0
17	9242.5
18	8477.0
19	8428.0
20	8379.0
21	8393.0
22	8407.0
23	8241.0
24	8075.0
25	8862.5
26	10162.0
27	10674.0
28	11789.5
29	12905.0
30	14757.5
31	16610.0
32	17979.0
33	19348.0
34	21395.5
35	23443.0
36	24841.0
37	26239.0
38	29023.5
39	31808.0
40	36988.0
41	42168.0
42	53569.0
43	64970.0
44	83398.0
45	101826.0
46	146025.0
47	190224.0
48	223182.5
49	256141.0
50	239219.0
51	222297.0
52	166822.0
53	111347.0
54	80149.5
55	48952.0
56	35521.0
57	22090.0
58	17237.5
59	12385.0
60	10517.5
61	8650.0
62	7527.0
63	6404.0
64	5364.5
65	4325.0
66	3468.5
67	2612.0
68	2035.5
69	1459.0
70	1145.5
71	832.0
72	693.5
73	555.0
74	432.0
75	253.5
76	198.0
77	157.5
78	117.0
79	91.5
80	66.0
81	41.0
82	16.0
83	12.5
84	9.0
85	4.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1389982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.91575303231235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.65873524273238	21.941224666960746
2	12.748848737956878	8.392759130097913
3	5.963630676703011	5.888921835908343
4	3.4687096229821996	4.567007571635495
5	2.2863387663795356	3.762828109117524
6	1.7017102532664667	3.3607844657443633
7	1.2152432705329137	2.800045315692865
8	0.9632566900074413	2.5365055452006073
9	0.7793136813546157	2.308652700315362
>10	3.8659635732896116	21.588675677520456
>50	0.21593600697006324	4.9523640475334245
>100	0.11366828705731992	7.118049151769027
>500	0.009210274957538614	2.0688296044594625
>1k	0.008087070694424147	4.004119544683113
>5k	6.73922557868679E-4	1.4474191181705556
>10k+	6.73922557868679E-4	3.2618135151906884
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22799	1.6402370678181444	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	10812	0.777851799519706	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	10502	0.7555493524376575	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	7893	0.5678490800600295	TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	6101	0.43892654725025215	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	5581	0.4015159908545578	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3152	0.22676552646005488	No Hit
ACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	3052	0.2195711886916521	TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	2505	0.18021816109848904	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	2344	0.1686352772913606	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	2020	0.14532562292173568	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1950	0.14028958648385376	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC	1937	0.1393543225739614	No Hit
GCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	1921	0.13820322853101694	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	1793	0.12899447618746143	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1712	0.12316706259505518	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	1580	0.11367053674076355	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	1528	0.10992948110119412	No Hit
GACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	1493	0.10741146288225314	No Hit
AGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	1436	0.10331069035426359	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	1418	0.10201570955595109	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.194337768402757E-5	0.0	0.0	0.6014466374384704	0.0
2	7.194337768402757E-5	0.0	0.0	2.9352898095083244	0.0
3	7.194337768402757E-5	0.0	0.0	4.0571028977353665	0.0
4	7.194337768402757E-5	0.0	0.0	5.654245882320779	0.0
5	7.194337768402757E-5	0.0	0.0	10.258190393832438	0.0
6	7.194337768402757E-5	0.0	0.0	13.022254964452777	0.0
7	7.194337768402757E-5	0.0	0.0	15.803226228828862	0.0
8	7.194337768402757E-5	0.0	0.0	19.97860403947677	0.0
9	7.194337768402757E-5	0.0	0.0	21.32135524057146	0.0
10	7.194337768402757E-5	0.0	0.0	25.273348863510464	0.0
11	7.194337768402757E-5	0.0	0.0	31.360981652999822	0.0
12	7.194337768402757E-5	0.0	0.0	36.07694200356551	0.0
13	7.194337768402757E-5	0.0	0.0	37.840202247223345	0.0
14	7.194337768402757E-5	0.0	0.0	38.50905982955175	0.0
15	7.194337768402757E-5	0.0	0.0	39.76605452444708	0.0
16	7.194337768402757E-5	0.0	0.0	42.24155420717678	0.0
17	7.194337768402757E-5	0.0	0.0	45.51728007988593	0.0
18	7.194337768402757E-5	0.0	0.0	48.511275685584415	0.0
19	7.194337768402757E-5	0.0	0.0	50.427919210464594	0.0
20	7.194337768402757E-5	0.0	0.0	52.223482030702556	0.0
21	7.194337768402757E-5	0.0	0.0	54.223867647206944	0.0
22	7.194337768402757E-5	0.0	0.0	56.349434740881534	0.0
23	7.194337768402757E-5	0.0	0.0	58.25053849618197	0.0
24	7.194337768402757E-5	0.0	0.0	59.54321710640857	0.0
25	7.194337768402757E-5	0.0	0.0	60.55711512810957	0.0
26	7.194337768402757E-5	0.0	0.0	61.370794729715925	0.0
27	7.194337768402757E-5	0.0	0.0	62.105984106269005	0.0
28	7.194337768402757E-5	0.0	0.0	62.768726501494264	0.0
29	7.194337768402757E-5	0.0	0.0	63.38326683367123	0.0
30	7.194337768402757E-5	0.0	0.0	64.04198039974618	0.0
31	7.194337768402757E-5	0.0	0.0	64.567526773728	0.0
32	7.194337768402757E-5	0.0	0.0	64.99371934312819	0.0
33	7.194337768402757E-5	0.0	0.0	65.37840058360467	0.0
34	1.4388675536805513E-4	0.0	0.0	65.74200241441976	0.0
35	1.4388675536805513E-4	0.0	0.0	66.10315817039357	0.0
36	2.158301330520827E-4	0.0	0.0	66.43776682000198	0.0
37	2.158301330520827E-4	0.0	0.0	66.7399290062749	0.0
38	2.158301330520827E-4	0.0	0.0	67.04835026640633	0.0
39	2.158301330520827E-4	0.0	0.0	67.36375003417311	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	20	7.0337974E-4	45.0	25
TTAGACG	25	3.8912287E-5	45.0	1
TAACGCG	25	3.8912287E-5	45.0	1
TCGCTAG	20	7.0337974E-4	45.0	1
ACTAACG	25	3.8912287E-5	45.0	1
ATAACGT	35	1.2121745E-7	45.0	31
TAGGACG	25	3.8912287E-5	45.0	1
TTACCGG	20	7.0337974E-4	45.0	2
CGATAGA	20	7.0337974E-4	45.0	12
CGCCCAA	20	7.0337974E-4	45.0	37
TAGTACG	45	3.8562575E-10	45.0	1
AGTCACG	30	2.1658325E-6	44.999996	1
TGTATCG	30	2.1658325E-6	44.999996	1
TAACGAG	30	2.1658325E-6	44.999996	1
GTTCGCG	30	2.1658325E-6	44.999996	1
CGTTATT	665	0.0	43.646614	1
CGTTTTT	9125	0.0	43.569866	1
CTACGAA	80	0.0	42.1875	11
ACGTAAG	75	0.0	42.0	1
AGTACGG	135	0.0	41.666668	2
>>END_MODULE