SRR2935185.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277527
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7182	2.587856316682701	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4956	1.7857721951377703	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2078	0.7487559768959416	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	2075	0.7476750009908946	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	1784	0.6428203382013281	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	1762	0.6348931815643163	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	1176	0.42374255477845396	TruSeq Adapter, Index 19 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1145	0.4125724704263009	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1111	0.40032141016910067	No Hit
TCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	964	0.3473535908217939	TruSeq Adapter, Index 13 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.28826024134588707	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.25150706057428646	No Hit
ACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	687	0.24754348225578052	TruSeq Adapter, Index 19 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.239976650920451	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCG	545	0.19637728941688556	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.1826849279529559	No Hit
ACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	372	0.13404101222583747	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGC	358	0.12899645800228446	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.12791548209723738	No Hit
GCCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	307	0.11061986761648417	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	294	0.10593563869461349	TruSeq Adapter, Index 19 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	25	3.8831335E-5	45.000004	6
CCCTCGT	25	3.8831335E-5	45.000004	14
ATATGAG	25	3.8831335E-5	45.000004	37
AATATGG	35	1.2074815E-7	45.000004	2
CGGGACG	25	3.8831335E-5	45.000004	6
CAAGCGG	35	1.2074815E-7	45.000004	2
TTAAACG	25	3.8831335E-5	45.000004	37
TTACGCG	35	1.2074815E-7	45.000004	1
ACGTAAG	25	3.8831335E-5	45.000004	1
AGTCGTG	25	3.8831335E-5	45.000004	1
ATCGAGG	25	3.8831335E-5	45.000004	2
TCACGAC	20	7.0240296E-4	45.0	25
TATCAGG	20	7.0240296E-4	45.0	2
ATGGGCG	20	7.0240296E-4	45.0	5
CGTGGGT	20	7.0240296E-4	45.0	4
CTCACGA	20	7.0240296E-4	45.0	24
CAAACGG	20	7.0240296E-4	45.0	2
TTGTGCG	20	7.0240296E-4	45.0	1
GATCGAC	20	7.0240296E-4	45.0	10
CGCGGGT	20	7.0240296E-4	45.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content